Restriction map for
pF1KE1722
(Circular) (Six-base) MAPSORT of: pF1KE1722.seq Check: 2449 from: 1 to: 3812
pF1KE1722 3812 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 790 790
Size: 3812
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 2069 2816 3084 1637
Size: 432 747 268 2365
Fragments arranged by size:
2365 747 432 268
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AhdI GACnn_n'nnGTC
Cuts at: 483 483
Size: 3812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 435 1117 2684 435
Size: 682 1567 1563
Fragments arranged by size:
1567 1563 682
ApaI G_GGCC'C
Cuts at: 420 526 633 420
Size: 106 107 3599
Fragments arranged by size:
3599 107 106
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 816 3165 3421 816
Size: 2349 256 1207
Fragments arranged by size:
2349 1207 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 832 832
Size: 3812
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 130 250 767 828 1242 1490 1525 130
Size: 120 517 61 414 248 35 2417
Fragments arranged by size:
2417 517 414 248 120 61 35
BanII G_rGCy'C
Cuts at: 163 192 420 526 633 737 826 1856 163
Size: 29 228 106 107 104 89 1030 2119
Fragments arranged by size:
2119 1030 228 107 106 104 89 29
BbeI G_GCGC'C
Cuts at: 254 1246 1494 254
Size: 992 248 2572
Fragments arranged by size:
2572 992 248
BbsI GAAGACnn'nnnn_
Cuts at: 456 766 456
Size: 310 3502
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BglI GCCn_nnn'nGGC
Cuts at: 791 791
Size: 3812
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 420 526 633 1437 1530 2586 420
Size: 106 107 804 93 1056 1646
Fragments arranged by size:
1646 1056 804 107 106 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 605
Size: 3812
Bpu10I CC'TnA_GC
Cuts at: 191 191
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1867 2359 2657 988
Size: 879 492 298 2143
Fragments arranged by size:
2143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 637 3516 637
Size: 2879 933
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 251 461 1243 1491 2193 3689 251
Size: 210 782 248 702 1496 374
Fragments arranged by size:
1496 782 702 374 248 210
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 688
Size: 3812
BseYI C'CCAG_C
Cuts at: 456 536 623 1237 1995 2572 3068 456
Size: 80 87 614 758 577 496 1200
Fragments arranged by size:
1200 758 614 577 496 87 80
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 826 1604 1794 2586 826
Size: 778 190 792 2052
Fragments arranged by size:
2052 792 778 190
BsmI GAATG_Cn'
Cuts at: 374 374
Size: 3812
BsmBI CGTCTCn'nnnn_
Cuts at: 491 491
Size: 3812
Bsp1286I G_dGCh'C
Cuts at: 163 192 420 526 633 737 826 1437 1530
Size: 29 228 106 107 104 89 611 93
Cuts at: 1530 1604 1794 1856 2586 163
Size: 74 190 62 730 1389
Fragments arranged by size:
1389 730 611 228 190 107 106 104
93 89 74 62 29
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 862 1076 1378 1759 790
Size: 72 214 302 381 2843
Fragments arranged by size:
2843 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 550 2083 2441 550
Size: 1533 358 1921
Fragments arranged by size:
1921 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstXI CCAn_nnnn'nTGG
Cuts at: 261 261
Size: 3812
BstYI r'GATC_y
Cuts at: 1 701 837 1304 1327 1662 1908 2920 3006
Size: 700 136 467 23 335 246 1012 86
Cuts at: 3006 3018 3364 1
Size: 12 346 449
Fragments arranged by size:
1012 700 467 449 346 335 246 136
86 23 12
BtgI C'CryG_G
Cuts at: 107 722 737 107
Size: 615 15 3182
Fragments arranged by size:
3182 615 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 578 1055 578
Size: 477 3335
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DraIII CAC_nnn'GTG
Cuts at: 639 639
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 149 650 803 1397 1571 1962 1989 3334 149
Size: 501 153 594 174 391 27 1345 627
Fragments arranged by size:
1345 627 594 501 391 174 153 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1637 2069 2816 3084 605
Size: 1032 432 747 268 1333
Fragments arranged by size:
1333 1032 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 416 417 522 523 629 746 951 416
Size: 1 105 1 106 117 205 3277
Fragments arranged by size:
3277 205 117 106 105 1 1
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 254 1246 1494 2516 3495 254
Size: 992 248 1022 979 571
Fragments arranged by size:
1022 992 979 571 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 469 498 501 1654 1684 1686 1716 466
Size: 3 29 3 1153 30 2 30 2562
Fragments arranged by size:
2562 1153 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 353 851 353
Size: 498 3314
KasI G'GCGC_C
Cuts at: 250 1242 1490 250
Size: 992 248 2572
Fragments arranged by size:
2572 992 248
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 151 1573 151
Size: 1422 2390
MslI CAynn'nnrTG
Cuts at: 1801 1928 1801
Size: 127 3685
MspA1I CmG'CkG
Cuts at: 222 288 498 601 623 923 1237 1597 2610
Size: 66 210 103 22 300 314 360 1013
Cuts at: 2610 2855 222
Size: 245 1179
Fragments arranged by size:
1179 1013 360 314 300 245 210 103
66 22
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 251 1243 1491 251
Size: 992 248 2572
Fragments arranged by size:
2572 992 248
NcoI C'CATG_G
Cuts at: 107 722 737 107
Size: 615 15 3182
Fragments arranged by size:
3182 615 15
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PflMI CCAn_nnn'nTGG
Cuts at: 435 639 435
Size: 204 3608
PfoI T'CCnGG_A
Cuts at: 329 713 329
Size: 384 3428
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PpuMI rG'GwC_Cy
Cuts at: 746 746
Size: 3812
PspOMI G'GGCC_C
Cuts at: 416 522 629 416
Size: 106 107 3599
Fragments arranged by size:
3599 107 106
PstI C_TGCA'G
Cuts at: 503 609 859 1544 503
Size: 106 250 685 2771
Fragments arranged by size:
2771 685 250 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 222 288 498 623 1237 1597 222
Size: 66 210 125 614 360 2437
Fragments arranged by size:
2437 614 360 210 125 66
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 499 605 750 855 1540 2533 2724 33
Size: 466 106 145 105 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 191 145 106 105
SfoI GGC'GCC
Cuts at: 252 1244 1492 252
Size: 992 248 2572
Fragments arranged by size:
2572 992 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 967 1882 2374 2636 967
Size: 915 492 262 2143
Fragments arranged by size:
2143 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StuI AGG'CCT
Cuts at: 679 679
Size: 3812
StyI C'CwwG_G
Cuts at: 107 722 737 946 1300 107
Size: 615 15 209 354 2619
Fragments arranged by size:
2619 615 354 209 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 1528 1987 2196 1395
Size: 133 459 209 3011
Fragments arranged by size:
3011 459 209 133
TatI w'GTAC_w
Cuts at: 230 3327 230
Size: 3097 715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI