Restriction map for
pF1KE1731
(Circular) (Six-base) MAPSORT of: pF1KE1731.seq Check: 4032 from: 1 to: 3830
pF1KE1731 3830 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 212 868 212
Size: 656 3174
Acc65I G'GTAC_C
Cuts at: 392 846 392
Size: 454 3376
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1655 2087 2834 3102 1655
Size: 432 747 268 2383
Fragments arranged by size:
2383 747 432 268
AflIII A'CryG_T
Cuts at: 569 1182 2286 3263 569
Size: 613 1104 977 1136
Fragments arranged by size:
1136 1104 977 613
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2702 1135
Size: 1567 2263
ApaI G_GGCC'C
Cuts at: 169 537 169
Size: 368 3462
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 834 3183 3439 834
Size: 2349 256 1225
Fragments arranged by size:
2349 1225 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 285 850 285
Size: 565 3265
BamHI G'GATC_C
Cuts at: 467 855 467
Size: 388 3442
BanI G'GyrC_C
Cuts at: 231 239 392 846 1260 1508 1543 231
Size: 8 153 454 414 248 35 2518
Fragments arranged by size:
2518 454 414 248 153 35 8
BanII G_rGCy'C
Cuts at: 149 169 177 537 757 773 802 844 1874
Size: 20 8 360 220 16 29 42 1030
Cuts at: 1874 149
Size: 2105
Fragments arranged by size:
2105 1030 360 220 42 29 20 16
8
BbeI G_GCGC'C
Cuts at: 235 243 1264 1512 235
Size: 8 1021 248 2553
Fragments arranged by size:
2553 1021 248 8
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglI GCCn_nnn'nGGC
Cuts at: 153 206 735 153
Size: 53 529 3248
Fragments arranged by size:
3248 529 53
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 133 942 133
Size: 809 3021
Bme1580I G_kGCm'C
Cuts at: 169 537 1455 1548 2604 169
Size: 368 918 93 1056 1395
Fragments arranged by size:
1395 1056 918 368 93
BmrI ACTGGGnnnn_n'
Cuts at: 805 1042 1123 1458 3602 805
Size: 237 81 335 2144 1033
Fragments arranged by size:
2144 1033 335 237 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 259 331 787 829 163
Size: 96 72 456 42 3164
Fragments arranged by size:
3164 456 96 72 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2377 2675 1006
Size: 879 492 298 2161
Fragments arranged by size:
2161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 382 3534 382
Size: 3152 678
BsaAI yAC'GTr
Cuts at: 491 570 1183 491
Size: 79 613 3138
Fragments arranged by size:
3138 613 79
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 209 232 240 1261 1509 2211 3707 209
Size: 23 8 1021 248 702 1496 332
Fragments arranged by size:
1496 1021 702 332 248 23 8
BsaWI w'CCGG_w
Cuts at: 509 1019 1540 2218 2492 2639 3050 509
Size: 510 521 678 274 147 411 1289
Fragments arranged by size:
1289 678 521 510 411 274 147
BseYI C'CCAG_C
Cuts at: 477 547 803 1255 2013 2590 3086 477
Size: 70 256 452 758 577 496 1221
Fragments arranged by size:
1221 758 577 496 452 256 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 265
Size: 3830
BsiEI CG_ry'CG
Cuts at: 104 201 294 1372 1418 2626 104
Size: 97 93 1078 46 1208 1308
Fragments arranged by size:
1308 1208 1078 97 93 46
BsiHKAI G_wGCw'C
Cuts at: 221 437 844 1622 1812 2604 221
Size: 216 407 778 190 792 1447
Fragments arranged by size:
1447 792 778 407 216 190
Bsp1286I G_dGCh'C
Cuts at: 149 169 177 221 437 537 757 773 802
Size: 20 8 44 216 100 220 16 29
Cuts at: 802 844 1455 1548 1622 1812 1874 2604 149
Size: 42 611 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 220 216 190 100 93
74 62 44 42 29 20 16 8
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 3006 3006
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1094 1396 1777 880
Size: 214 302 381 2933
Fragments arranged by size:
2933 381 302 214
BsrBI CCG'CTC
Cuts at: 50 290 465 2122 2176 3660 3825 50
Size: 240 175 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 240 175 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 539 590 1828 2009 2218 539
Size: 51 1238 181 209 2151
Fragments arranged by size:
2151 1238 209 181 51
BssSI C'ACGA_G
Cuts at: 472 670 2101 2459 472
Size: 198 1431 358 1843
Fragments arranged by size:
1843 1431 358 198
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstYI r'GATC_y
Cuts at: 1 467 855 1322 1345 1680 1926 2938 3024
Size: 466 388 467 23 335 246 1012 86
Cuts at: 3024 3036 3382 1
Size: 12 346 449
Fragments arranged by size:
1012 467 466 449 388 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 201 107
Size: 94 3736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 203 1780 1961 2124 203
Size: 1577 181 163 1909
Fragments arranged by size:
1909 1577 181 163
BtsI GCAGTG_nn'
Cuts at: 353 1073 353
Size: 720 3110
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2394 1536
Size: 858 2972
EaeI y'GGCC_r
Cuts at: 198 291 365 398 1415 1589 1980 2007 3352
Size: 93 74 33 1017 174 391 27 1345
Cuts at: 3352 198
Size: 676
Fragments arranged by size:
1345 1017 676 391 174 93 74 33
27
EagI C'GGCC_G
Cuts at: 198 291 1415 198
Size: 93 1124 2613
Fragments arranged by size:
2613 1124 93
EarI CTCTTCn'nnn_
Cuts at: 385 1853 2063 385
Size: 1468 210 2152
Fragments arranged by size:
2152 1468 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2348 2494 3640 2038
Size: 310 146 1146 2228
Fragments arranged by size:
2228 1146 310 146
EcoICRI GAG'CTC
Cuts at: 842 842
Size: 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 259 331 787 829 1655 2087 2834 3102
Size: 96 72 456 42 826 432 747 268
Cuts at: 3102 163
Size: 891
Fragments arranged by size:
891 826 747 456 432 268 96 72
42
EcoO109I rG'GnC_Cy
Cuts at: 166 533 969 166
Size: 367 436 3027
Fragments arranged by size:
3027 436 367
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FseI GG_CCGG'CC
Cuts at: 594 594
Size: 3830
FspI TGC'GCA
Cuts at: 821 1611 821
Size: 790 3040
FspAI rTGC'GCAy
Cuts at: 821 821
Size: 3830
HaeII r_GCGC'y
Cuts at: 235 243 323 446 554 1264 1512 2534 3513
Size: 8 80 123 108 710 248 1022 979
Cuts at: 3513 235
Size: 552
Fragments arranged by size:
1022 979 710 552 248 123 108 80
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1672 1702 1704 1734 1672
Size: 30 2 30 3768
Fragments arranged by size:
3768 30 30 2
HincII GTy'rAC
Cuts at: 300 869 300
Size: 569 3261
KasI G'GCGC_C
Cuts at: 231 239 1260 1508 231
Size: 8 1021 248 2553
Fragments arranged by size:
2553 1021 248 8
KpnI G_GTAC'C
Cuts at: 396 850 396
Size: 454 3376
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2685 2501
Size: 184 3646
MscI TGG'CCA
Cuts at: 367 400 1591 367
Size: 33 1191 2606
Fragments arranged by size:
2606 1191 33
MslI CAynn'nnrTG
Cuts at: 1819 1946 1819
Size: 127 3703
MspA1I CmG'CkG
Cuts at: 163 279 807 941 1255 1615 2628 2873 163
Size: 116 528 134 314 360 1013 245 1120
Fragments arranged by size:
1120 1013 528 360 314 245 134 116
NaeI GCC'GGC
Cuts at: 541 592 2011 541
Size: 51 1419 2360
Fragments arranged by size:
2360 1419 51
NarI GG'CG_CC
Cuts at: 232 240 1261 1509 232
Size: 8 1021 248 2553
Fragments arranged by size:
2553 1021 248 8
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 539 590 2009 539
Size: 51 1419 2360
Fragments arranged by size:
2360 1419 51
NotI GC'GGCC_GC
Cuts at: 291 291
Size: 3830
NspI r_CATG'y
Cuts at: 883 1383 2290 883
Size: 500 907 2423
Fragments arranged by size:
2423 907 500
PasI CC'CwG_GG
Cuts at: 169 169
Size: 3830
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PflMI CCAn_nnn'nTGG
Cuts at: 477 675 477
Size: 198 3632
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PspOMI G'GGCC_C
Cuts at: 165 533 165
Size: 368 3462
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 307 339 307
Size: 32 3798
PstI C_TGCA'G
Cuts at: 877 1562 877
Size: 685 3145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 279 807 1255 1615 279
Size: 528 448 360 2494
Fragments arranged by size:
2494 528 448 360
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 844 844
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 324 438 688 873 1558 2551 2742 33
Size: 291 114 250 185 685 993 191 1121
Fragments arranged by size:
1121 993 685 291 250 191 185 114
SfoI GGC'GCC
Cuts at: 233 241 1262 1510 233
Size: 8 1021 248 2553
Fragments arranged by size:
2553 1021 248 8
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 985 1900 2392 2654 985
Size: 915 492 262 2161
Fragments arranged by size:
2161 915 492 262
SnaBI TAC'GTA
Cuts at: 491 491
Size: 3830
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StyI C'CwwG_G
Cuts at: 107 222 964 1318 107
Size: 115 742 354 2619
Fragments arranged by size:
2619 742 354 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 3345 3345
Size: 3830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 3798
Tth111I GACn'n_nGTC
Cuts at: 516 1627 516
Size: 1111 2719
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PmeI PpiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI