Restriction map for
pF1KE1747
(Circular) (Six-base) MAPSORT of: pF1KE1747.seq Check: 4292 from: 1 to: 3866
pF1KE1747 3866 bp
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 272 272
Size: 3866
AatII G_ACGT'C
Cuts at: 485 485
Size: 3866
AccI GT'mk_AC
Cuts at: 485 904 485
Size: 419 3447
Acc65I G'GTAC_C
Cuts at: 882 882
Size: 3866
AclI AA'CG_TT
Cuts at: 3706 3706
Size: 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1691 2123 2870 3138 706
Size: 985 432 747 268 1434
Fragments arranged by size:
1434 985 747 432 268
AflIII A'CryG_T
Cuts at: 1218 2322 3299 1218
Size: 1104 977 1785
Fragments arranged by size:
1785 1104 977
AgeI A'CCGG_T
Cuts at: 2254 2254
Size: 3866
AlwNI CAG_nnn'CTG
Cuts at: 131 155 465 579 1171 2738 131
Size: 24 310 114 592 1567 1259
Fragments arranged by size:
1567 1259 592 310 114 24
ApaI G_GGCC'C
Cuts at: 120 699 829 120
Size: 579 130 3157
Fragments arranged by size:
3157 579 130
ApaLI G'TGCA_C
Cuts at: 2636 2636
Size: 3866
ApoI r'AATT_y
Cuts at: 870 3219 3475 870
Size: 2349 256 1261
Fragments arranged by size:
2349 1261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3866
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
AvaI C'yCGr_G
Cuts at: 692 738 886 692
Size: 46 148 3672
Fragments arranged by size:
3672 148 46
BamHI G'GATC_C
Cuts at: 891 891
Size: 3866
BanI G'GyrC_C
Cuts at: 882 1296 1544 1579 882
Size: 414 248 35 3169
Fragments arranged by size:
3169 414 248 35
BanII G_rGCy'C
Cuts at: 120 699 737 829 880 1910 120
Size: 579 38 92 51 1030 2076
Fragments arranged by size:
2076 1030 579 92 51 38
BbeI G_GCGC'C
Cuts at: 1300 1548 1300
Size: 248 3618
BciVI GTATCCnnnnn_n'
Cuts at: 1764 2525 3866 1764
Size: 761 1341 1764
Fragments arranged by size:
1764 1341 761
BclI T'GATC_A
Cuts at: 329 1386 329
Size: 1057 2809
BglI GCCn_nnn'nGGC
Cuts at: 833 833
Size: 3866
BglII A'GATC_T
Cuts at: 1381 3418 1381
Size: 2037 1829
BlpI GC'TnA_GC
Cuts at: 978 978
Size: 3866
Bme1580I G_kGCm'C
Cuts at: 120 324 699 829 1491 1584 2640 120
Size: 204 375 130 662 93 1056 1346
Fragments arranged by size:
1346 1056 662 375 204 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 567 1078 1159 1494 3638 567
Size: 511 81 335 2144 795
Fragments arranged by size:
2144 795 511 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 675 2233 675
Size: 1558 2308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 192
Size: 3866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1921 2413 2711 1042
Size: 879 492 298 2197
Fragments arranged by size:
2197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3570 3570
Size: 3866
BsaAI yAC'GTr
Cuts at: 1219 1219
Size: 3866
BsaBI GATnn'nnATC
Cuts at: 6 1380 3417 6
Size: 1374 2037 455
Fragments arranged by size:
2037 1374 455
BsaHI Gr'CG_yC
Cuts at: 482 1297 1545 2247 3743 482
Size: 815 248 702 1496 605
Fragments arranged by size:
1496 815 702 605 248
BsaWI w'CCGG_w
Cuts at: 1055 1576 2254 2528 2675 3086 1055
Size: 521 678 274 147 411 1835
Fragments arranged by size:
1835 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 719 749 719
Size: 30 3836
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 485 485
Size: 3866
BseYI C'CCAG_C
Cuts at: 212 814 1291 2049 2626 3122 212
Size: 602 477 758 577 496 956
Fragments arranged by size:
956 758 602 577 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 168
Size: 3866
BsiEI CG_ry'CG
Cuts at: 104 798 1408 1454 2662 104
Size: 694 610 46 1208 1308
Fragments arranged by size:
1308 1208 694 610 46
BsiHKAI G_wGCw'C
Cuts at: 737 880 1658 1848 2640 737
Size: 143 778 190 792 1963
Fragments arranged by size:
1963 792 778 190 143
Bsp1286I G_dGCh'C
Cuts at: 120 324 699 737 829 880 1491 1584 1658
Size: 204 375 38 92 51 611 93 74
Cuts at: 1658 1848 1910 2640 120
Size: 190 62 730 1346
Fragments arranged by size:
1346 730 611 375 204 190 93 92
74 62 51 38
BspEI T'CCGG_A
Cuts at: 3086 3086
Size: 3866
BspHI T'CATG_A
Cuts at: 3042 3042
Size: 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 437 916 1130 1432 1813 272
Size: 165 479 214 302 381 2325
Fragments arranged by size:
2325 479 381 302 214 165
BsrBI CCG'CTC
Cuts at: 50 666 2158 2212 3696 3861 50
Size: 616 1492 54 1484 165 55
Fragments arranged by size:
1492 1484 616 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1778 1778
Size: 3866
BsrFI r'CCGG_y
Cuts at: 1864 2045 2254 1864
Size: 181 209 3476
Fragments arranged by size:
3476 209 181
BsrGI T'GTAC_A
Cuts at: 233 233
Size: 3866
BssSI C'ACGA_G
Cuts at: 2137 2495 2137
Size: 358 3508
BstAPI GCAn_nnn'nTGC
Cuts at: 155 1243 155
Size: 1088 2778
BstBI TT'CG_AA
Cuts at: 2227 2227
Size: 3866
BstXI CCAn_nnnn'nTGG
Cuts at: 280 292 280
Size: 12 3854
BstYI r'GATC_y
Cuts at: 1 269 596 891 1358 1381 1716 1962 2974
Size: 268 327 295 467 23 335 246 1012
Cuts at: 2974 3060 3072 3418 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 327 295 268
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 124 124
Size: 3866
BtgI C'CryG_G
Cuts at: 628 628
Size: 3866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 1997 2160 1816
Size: 181 163 3522
Fragments arranged by size:
3522 181 163
BtsI GCAGTG_nn'
Cuts at: 196 1109 196
Size: 913 2953
DraI TTT'AAA
Cuts at: 865 865
Size: 3866
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2430 1572
Size: 858 3008
EaeI y'GGCC_r
Cuts at: 282 764 1451 1625 2016 2043 3388 282
Size: 482 687 174 391 27 1345 760
Fragments arranged by size:
1345 760 687 482 391 174 27
EagI C'GGCC_G
Cuts at: 1451 1451
Size: 3866
EarI CTCTTCn'nnn_
Cuts at: 526 1889 2099 526
Size: 1363 210 2293
Fragments arranged by size:
2293 1363 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2074 2384 2530 3676 2074
Size: 310 146 1146 2264
Fragments arranged by size:
2264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 735 878 735
Size: 143 3723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 706 1691 2123 2870 3138 192
Size: 514 985 432 747 268 920
Fragments arranged by size:
985 920 747 514 432 268
EcoO109I rG'GnC_Cy
Cuts at: 116 669 1005 116
Size: 553 336 2977
Fragments arranged by size:
2977 553 336
EcoRI G'AATT_C
Cuts at: 870 870
Size: 3866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 555 1885 555
Size: 1330 2536
FspI TGC'GCA
Cuts at: 1647 1647
Size: 3866
HaeII r_GCGC'y
Cuts at: 650 746 1300 1548 2570 3549 650
Size: 96 554 248 1022 979 967
Fragments arranged by size:
1022 979 967 554 248 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1708 1738 1740 1770 1708
Size: 30 2 30 3804
Fragments arranged by size:
3804 30 30 2
HincII GTy'rAC
Cuts at: 905 905
Size: 3866
KasI G'GCGC_C
Cuts at: 1296 1544 1296
Size: 248 3618
KpnI G_GTAC'C
Cuts at: 886 886
Size: 3866
MluI A'CGCG_T
Cuts at: 3299 3299
Size: 3866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 477 2537 2721 477
Size: 2060 184 1622
Fragments arranged by size:
2060 1622 184
MscI TGG'CCA
Cuts at: 284 766 1627 284
Size: 482 861 2523
Fragments arranged by size:
2523 861 482
MslI CAynn'nnrTG
Cuts at: 278 290 1855 1982 278
Size: 12 1565 127 2162
Fragments arranged by size:
2162 1565 127 12
MspA1I CmG'CkG
Cuts at: 212 977 1291 1651 2664 2909 212
Size: 765 314 360 1013 245 1169
Fragments arranged by size:
1169 1013 765 360 314 245
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 3866
NarI GG'CG_CC
Cuts at: 1297 1545 1297
Size: 248 3618
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 3866
NspI r_CATG'y
Cuts at: 919 1419 2326 919
Size: 500 907 2459
Fragments arranged by size:
2459 907 500
PciI A'CATG_T
Cuts at: 2322 2322
Size: 3866
PflMI CCAn_nnn'nTGG
Cuts at: 465 465
Size: 3866
PfoI T'CCnGG_A
Cuts at: 583 583
Size: 3866
PmeI GTTT'AAAC
Cuts at: 865 865
Size: 3866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3834
PpuMI rG'GwC_Cy
Cuts at: 669 669
Size: 3866
PspOMI G'GGCC_C
Cuts at: 116 695 825 116
Size: 579 130 3157
Fragments arranged by size:
3157 579 130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 559 591 559
Size: 32 3834
PstI C_TGCA'G
Cuts at: 160 269 448 913 1598 160
Size: 109 179 465 685 2428
Fragments arranged by size:
2428 685 465 179 109
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3866
PvuII CAG'CTG
Cuts at: 212 1291 1651 212
Size: 1079 360 2427
Fragments arranged by size:
2427 1079 360
RsrII CG'GwC_CG
Cuts at: 2061 2061
Size: 3866
SacI G_AGCT'C
Cuts at: 737 880 737
Size: 143 3723
SalI G'TCGA_C
Cuts at: 903 903
Size: 3866
SanDI GG'GwC_CC
Cuts at: 669 669
Size: 3866
SapI GCTCTTCn'nnn_
Cuts at: 1889 2099 1889
Size: 210 3656
SbfI CC_TGCA'GG
Cuts at: 913 913
Size: 3866
ScaI AGT'ACT
Cuts at: 3383 3383
Size: 3866
SfcI C'TryA_G
Cuts at: 33 156 265 315 444 909 1594 2587 2778
Size: 123 109 50 129 465 685 993 191
Cuts at: 2778 33
Size: 1121
Fragments arranged by size:
1121 993 685 465 191 129 123 109
50
SfoI GGC'GCC
Cuts at: 1298 1546 1298
Size: 248 3618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
SmaI CCC'GGG
Cuts at: 694 888 694
Size: 194 3672
SmlI C'TyrA_G
Cuts at: 1021 1936 2428 2690 1021
Size: 915 492 262 2197
Fragments arranged by size:
2197 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3866
SphI G_CATG'C
Cuts at: 919 1419 919
Size: 500 3366
StyI C'CwwG_G
Cuts at: 1000 1354 1000
Size: 354 3512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1449 1582 2041 2250 1449
Size: 133 459 209 3065
Fragments arranged by size:
3065 459 209 133
TatI w'GTAC_w
Cuts at: 233 3381 233
Size: 3148 718
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 3383 3415 719
Size: 32 2632 32 1170
Fragments arranged by size:
2632 1170 32 32
Tth111I GACn'n_nGTC
Cuts at: 1663 1663
Size: 3866
XbaI T'CTAG_A
Cuts at: 897 897
Size: 3866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1361 1361
Size: 3866
XmaI C'CCGG_G
Cuts at: 692 886 692
Size: 194 3672
ZraI GAC'GTC
Cuts at: 483 483
Size: 3866
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI