Restriction map for
pF1KE1772
(Circular) (Six-base) MAPSORT of: pF1KE1772.seq Check: 6000 from: 1 to: 3950
pF1KE1772 3950 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 988 988
Size: 3950
Acc65I G'GTAC_C
Cuts at: 966 966
Size: 3950
AclI AA'CG_TT
Cuts at: 3790 3790
Size: 3950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 2207 2954 3222 1775
Size: 432 747 268 2503
Fragments arranged by size:
2503 747 432 268
AflIII A'CryG_T
Cuts at: 1302 2406 3383 1302
Size: 1104 977 1869
Fragments arranged by size:
1869 1104 977
AgeI A'CCGG_T
Cuts at: 2338 2338
Size: 3950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 806 806
Size: 3950
AlwNI CAG_nnn'CTG
Cuts at: 597 1255 2822 597
Size: 658 1567 1725
Fragments arranged by size:
1725 1567 658
ApaI G_GGCC'C
Cuts at: 305 327 335 421 305
Size: 22 8 86 3834
Fragments arranged by size:
3834 86 22 8
ApaLI G'TGCA_C
Cuts at: 2720 2720
Size: 3950
ApoI r'AATT_y
Cuts at: 954 3303 3559 954
Size: 2349 256 1345
Fragments arranged by size:
2349 1345 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
AvaI C'yCGr_G
Cuts at: 216 306 327 970 216
Size: 90 21 643 3196
Fragments arranged by size:
3196 643 90 21
BamHI G'GATC_C
Cuts at: 975 975
Size: 3950
BanI G'GyrC_C
Cuts at: 312 348 378 423 631 889 966 1380 1628
Size: 36 30 45 208 258 77 414 248
Cuts at: 1628 1663 312
Size: 35 2599
Fragments arranged by size:
2599 414 258 248 208 77 45 36
35 30
BanII G_rGCy'C
Cuts at: 305 327 335 421 506 964 1994 305
Size: 22 8 86 85 458 1030 2261
Fragments arranged by size:
2261 1030 458 86 85 22 8
BbeI G_GCGC'C
Cuts at: 316 427 635 893 1384 1632 316
Size: 111 208 258 491 248 2634
Fragments arranged by size:
2634 491 258 248 208 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3916
BciVI GTATCCnnnnn_n'
Cuts at: 1848 2609 3950 1848
Size: 761 1341 1848
Fragments arranged by size:
1848 1341 761
BclI T'GATC_A
Cuts at: 1470 1470
Size: 3950
BglI GCCn_nnn'nGGC
Cuts at: 300 300
Size: 3950
BglII A'GATC_T
Cuts at: 1465 3502 1465
Size: 2037 1913
BlpI GC'TnA_GC
Cuts at: 1062 1062
Size: 3950
Bme1580I G_kGCm'C
Cuts at: 305 327 335 421 1575 1668 2724 305
Size: 22 8 86 1154 93 1056 1531
Fragments arranged by size:
1531 1154 1056 93 86 22 8
BmrI ACTGGGnnnn_n'
Cuts at: 1162 1243 1578 3722 1162
Size: 81 335 2144 1390
Fragments arranged by size:
2144 1390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 2317 640
Size: 1677 2273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2005 2497 2795 1126
Size: 879 492 298 2281
Fragments arranged by size:
2281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3654 3654
Size: 3950
BsaAI yAC'GTr
Cuts at: 790 1303 790
Size: 513 3437
BsaBI GATnn'nnATC
Cuts at: 6 1464 3501 6
Size: 1458 2037 455
Fragments arranged by size:
2037 1458 455
BsaHI Gr'CG_yC
Cuts at: 313 424 632 890 1381 1629 2331 3827 313
Size: 111 208 258 491 248 702 1496 436
Fragments arranged by size:
1496 702 491 436 258 248 208 111
BsaWI w'CCGG_w
Cuts at: 1139 1660 2338 2612 2759 3170 1139
Size: 521 678 274 147 411 1919
Fragments arranged by size:
1919 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 797 827 797
Size: 30 3920
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 160 181 184 139
Size: 21 21 3 3905
Fragments arranged by size:
3905 21 21 3
BseYI C'CCAG_C
Cuts at: 207 517 1375 2133 2710 3206 207
Size: 310 858 758 577 496 951
Fragments arranged by size:
951 858 758 577 496 310
BsiEI CG_ry'CG
Cuts at: 104 465 1492 1538 2746 104
Size: 361 1027 46 1208 1308
Fragments arranged by size:
1308 1208 1027 361 46
BsiHKAI G_wGCw'C
Cuts at: 506 731 964 1742 1932 2724 506
Size: 225 233 778 190 792 1732
Fragments arranged by size:
1732 792 778 233 225 190
BsmBI CGTCTCn'nnnn_
Cuts at: 247 247
Size: 3950
Bsp1286I G_dGCh'C
Cuts at: 305 327 335 421 506 731 964 1575 1668
Size: 22 8 86 85 225 233 611 93
Cuts at: 1668 1742 1932 1994 2724 305
Size: 74 190 62 730 1531
Fragments arranged by size:
1531 730 611 233 225 190 93 86
85 74 62 22 8
BspEI T'CCGG_A
Cuts at: 3170 3170
Size: 3950
BspHI T'CATG_A
Cuts at: 3126 3126
Size: 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1000 1214 1516 1897 1000
Size: 214 302 381 3053
Fragments arranged by size:
3053 381 302 214
BsrBI CCG'CTC
Cuts at: 50 813 2242 2296 3780 3945 50
Size: 763 1429 54 1484 165 55
Fragments arranged by size:
1484 1429 763 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1862 1862
Size: 3950
BsrFI r'CCGG_y
Cuts at: 139 230 351 611 707 1948 2129 2338 139
Size: 91 121 260 96 1241 181 209 1751
Fragments arranged by size:
1751 1241 260 209 181 121 96 91
BssHII G'CGCG_C
Cuts at: 521 521
Size: 3950
BssSI C'ACGA_G
Cuts at: 2221 2579 2221
Size: 358 3592
BstAPI GCAn_nnn'nTGC
Cuts at: 726 1327 726
Size: 601 3349
BstBI TT'CG_AA
Cuts at: 2311 2311
Size: 3950
BstYI r'GATC_y
Cuts at: 1 975 1442 1465 1800 2046 3058 3144 3156
Size: 974 467 23 335 246 1012 86 12
Cuts at: 3156 3502 1
Size: 346 449
Fragments arranged by size:
1012 974 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 542 542
Size: 3950
BtgI C'CryG_G
Cuts at: 459 459
Size: 3950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1900 2081 2244 1900
Size: 181 163 3606
Fragments arranged by size:
3606 181 163
BtsI GCAGTG_nn'
Cuts at: 532 885 1193 532
Size: 353 308 3289
Fragments arranged by size:
3289 353 308
DraI TTT'AAA
Cuts at: 949 949
Size: 3950
DrdI GACnn_nn'nnGTC
Cuts at: 1656 2514 1656
Size: 858 3092
EaeI y'GGCC_r
Cuts at: 462 497 1535 1709 2100 2127 3472 462
Size: 35 1038 174 391 27 1345 940
Fragments arranged by size:
1345 1038 940 391 174 35 27
EagI C'GGCC_G
Cuts at: 462 1535 462
Size: 1073 2877
EarI CTCTTCn'nnn_
Cuts at: 125 158 1973 2183 125
Size: 33 1815 210 1892
Fragments arranged by size:
1892 1815 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 370 421 2158 2468 2614 3760 166
Size: 204 51 1737 310 146 1146 356
Fragments arranged by size:
1737 1146 356 310 204 146 51
EcoICRI GAG'CTC
Cuts at: 504 962 504
Size: 458 3492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 2207 2954 3222 1775
Size: 432 747 268 2503
Fragments arranged by size:
2503 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 745 897 745
Size: 152 3798
EcoO109I rG'GnC_Cy
Cuts at: 302 324 331 332 368 435 514 668 820
Size: 22 7 1 36 67 79 154 152
Cuts at: 820 1089 302
Size: 269 3163
Fragments arranged by size:
3163 269 154 152 79 67 36 22
7 1
EcoRI G'AATT_C
Cuts at: 954 954
Size: 3950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3950
FspI TGC'GCA
Cuts at: 1731 1731
Size: 3950
HaeII r_GCGC'y
Cuts at: 208 316 427 635 893 1384 1632 2654 3633
Size: 108 111 208 258 491 248 1022 979
Cuts at: 3633 208
Size: 525
Fragments arranged by size:
1022 979 525 491 258 248 208 111
108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1792 1822 1824 1854 1792
Size: 30 2 30 3888
Fragments arranged by size:
3888 30 30 2
HincII GTy'rAC
Cuts at: 989 989
Size: 3950
HindIII A'AGCT_T
Cuts at: 796 796
Size: 3950
KasI G'GCGC_C
Cuts at: 312 423 631 889 1380 1628 312
Size: 111 208 258 491 248 2634
Fragments arranged by size:
2634 491 258 248 208 111
KpnI G_GTAC'C
Cuts at: 970 970
Size: 3950
MluI A'CGCG_T
Cuts at: 3383 3383
Size: 3950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2621 2805 2621
Size: 184 3766
MscI TGG'CCA
Cuts at: 1711 1711
Size: 3950
MslI CAynn'nnrTG
Cuts at: 1939 2066 1939
Size: 127 3823
MspA1I CmG'CkG
Cuts at: 264 318 384 461 597 1061 1375 1735 2748
Size: 54 66 77 136 464 314 360 1013
Cuts at: 2748 2993 264
Size: 245 1221
Fragments arranged by size:
1221 1013 464 360 314 245 136 77
66 54
NaeI GCC'GGC
Cuts at: 141 353 613 2131 141
Size: 212 260 1518 1960
Fragments arranged by size:
1960 1518 260 212
NarI GG'CG_CC
Cuts at: 313 424 632 890 1381 1629 313
Size: 111 208 258 491 248 2634
Fragments arranged by size:
2634 491 258 248 208 111
NgoMIV G'CCGG_C
Cuts at: 139 351 611 2129 139
Size: 212 260 1518 1960
Fragments arranged by size:
1960 1518 260 212
NotI GC'GGCC_GC
Cuts at: 462 462
Size: 3950
NruI TCG'CGA
Cuts at: 774 774
Size: 3950
NspI r_CATG'y
Cuts at: 1003 1503 2410 1003
Size: 500 907 2543
Fragments arranged by size:
2543 907 500
PasI CC'CwG_GG
Cuts at: 430 450 430
Size: 20 3930
PciI A'CATG_T
Cuts at: 2406 2406
Size: 3950
PfoI T'CCnGG_A
Cuts at: 564 895 564
Size: 331 3619
PmeI GTTT'AAAC
Cuts at: 949 949
Size: 3950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3918
PpuMI rG'GwC_Cy
Cuts at: 368 435 668 820 368
Size: 67 233 152 3498
Fragments arranged by size:
3498 233 152 67
PshAI GACnn'nnGTC
Cuts at: 666 825 666
Size: 159 3791
PspOMI G'GGCC_C
Cuts at: 301 323 331 417 301
Size: 22 8 86 3834
Fragments arranged by size:
3834 86 22 8
PstI C_TGCA'G
Cuts at: 194 263 656 719 785 880 997 1682 194
Size: 69 393 63 66 95 117 685 2462
Fragments arranged by size:
2462 685 393 117 95 69 66 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3950
PvuII CAG'CTG
Cuts at: 264 1375 1735 264
Size: 1111 360 2479
Fragments arranged by size:
2479 1111 360
RsrII CG'GwC_CG
Cuts at: 2145 2145
Size: 3950
SacI G_AGCT'C
Cuts at: 506 964 506
Size: 458 3492
SacII CC_GC'GG
Cuts at: 462 462
Size: 3950
SalI G'TCGA_C
Cuts at: 987 987
Size: 3950
SanDI GG'GwC_CC
Cuts at: 668 668
Size: 3950
SapI GCTCTTCn'nnn_
Cuts at: 1973 2183 1973
Size: 210 3740
SbfI CC_TGCA'GG
Cuts at: 997 997
Size: 3950
ScaI AGT'ACT
Cuts at: 3467 3467
Size: 3950
SfcI C'TryA_G
Cuts at: 33 190 259 652 715 781 876 993 1678
Size: 157 69 393 63 66 95 117 685
Cuts at: 1678 2671 2862 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 393 191 157 117 95
69 66 63
SfoI GGC'GCC
Cuts at: 314 425 633 891 1382 1630 314
Size: 111 208 258 491 248 2634
Fragments arranged by size:
2634 491 258 248 208 111
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
SmaI CCC'GGG
Cuts at: 308 329 972 308
Size: 21 643 3286
Fragments arranged by size:
3286 643 21
SmlI C'TyrA_G
Cuts at: 1105 2020 2512 2774 1105
Size: 915 492 262 2281
Fragments arranged by size:
2281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3950
SphI G_CATG'C
Cuts at: 1003 1503 1003
Size: 500 3450
StyI C'CwwG_G
Cuts at: 1084 1438 1084
Size: 354 3596
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1533 1666 2125 2334 1533
Size: 133 459 209 3149
Fragments arranged by size:
3149 459 209 133
TatI w'GTAC_w
Cuts at: 3465 3465
Size: 3950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3467 3499 3467
Size: 32 3918
Tth111I GACn'n_nGTC
Cuts at: 1747 1747
Size: 3950
XbaI T'CTAG_A
Cuts at: 981 981
Size: 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1445 1445
Size: 3950
XmaI C'CCGG_G
Cuts at: 306 327 970 306
Size: 21 643 3286
Fragments arranged by size:
3286 643 21
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NruI NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsgI BsiWI BsmI BsrGI BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI