(Circular) (Six-base) MAPSORT of: pF1KE1779.seq  Check: 4847  from: 1  to: 3977



pF1KE1779  3977 bp

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:   1015    1015 
   Size:       3977
 
Acc65I G'GTAC_C

Cuts at:    993     993 
   Size:       3977
 
AclI AA'CG_TT

Cuts at:   3817    3817 
   Size:       3977
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    484     524    1802    2234    2981    3249     484 
   Size:         40    1278     432     747     268    1212

  Fragments arranged by size: 

                1278    1212     747     432     268      40
 
AflIII A'CryG_T

Cuts at:   1329    2433    3410    1329 
   Size:       1104     977    1896

  Fragments arranged by size: 

                1896    1104     977
 
AgeI A'CCGG_T

Cuts at:   2365    2365 
   Size:       3977
 
AlwNI CAG_nnn'CTG

Cuts at:   1282    2849    1282 
   Size:       1567    2410
 
ApaLI G'TGCA_C

Cuts at:   2747    2747 
   Size:       3977
 
ApoI r'AATT_y

Cuts at:    560     875     981    3330    3586     560 
   Size:        315     106    2349     256     951

  Fragments arranged by size: 

                2349     951     315     256     106
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3977
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3977
 
AvaI C'yCGr_G

Cuts at:    361     997     361 
   Size:        636    3341
 
BamHI G'GATC_C

Cuts at:   1002    1002 
   Size:       3977
 
BanI G'GyrC_C

Cuts at:    993    1407    1655    1690     993 
   Size:        414     248      35    3280

  Fragments arranged by size: 

                3280     414     248      35
 
BanII G_rGCy'C

Cuts at:    698     991    2021     698 
   Size:        293    1030    2654

  Fragments arranged by size: 

                2654    1030     293
 
BbeI G_GCGC'C

Cuts at:   1411    1659    1411 
   Size:        248    3729
 
BciVI GTATCCnnnnn_n'

Cuts at:   1875    2636    3977    1875 
   Size:        761    1341    1875

  Fragments arranged by size: 

                1875    1341     761
 
BclI T'GATC_A

Cuts at:   1497    1497 
   Size:       3977
 
BglII A'GATC_T

Cuts at:   1492    3529    1492 
   Size:       2037    1940
 
BlpI GC'TnA_GC

Cuts at:   1089    1089 
   Size:       3977
 
Bme1580I G_kGCm'C

Cuts at:    445    1602    1695    2751     445 
   Size:       1157      93    1056    1671

  Fragments arranged by size: 

                1671    1157    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1189    1270    1605    3749    1189 
   Size:         81     335    2144    1417

  Fragments arranged by size: 

                2144    1417     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2344    2344 
   Size:       3977
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    623     623 
   Size:       3977
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1153    2032    2524    2822    1153 
   Size:        879     492     298    2308

  Fragments arranged by size: 

                2308     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3681    3681 
   Size:       3977
 
BsaAI yAC'GTr

Cuts at:    219    1330     219 
   Size:       1111    2866
 
BsaBI GATnn'nnATC

Cuts at:      6    1491    3528       6 
   Size:       1485    2037     455

  Fragments arranged by size: 

                2037    1485     455
 
BsaHI Gr'CG_yC

Cuts at:   1408    1656    2358    3854    1408 
   Size:        248     702    1496    1531

  Fragments arranged by size: 

                1531    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    374    1166    1687    2365    2639    2786    3197     374 
   Size:        792     521     678     274     147     411    1154

  Fragments arranged by size: 

                1154     792     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1402    2160    2737    3233    1402 
   Size:        758     577     496    2146

  Fragments arranged by size: 

                2146     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1519    1565    2773     104 
   Size:       1415      46    1208    1308

  Fragments arranged by size: 

                1415    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    698     991    1769    1959    2751     698 
   Size:        293     778     190     792    1924

  Fragments arranged by size: 

                1924     792     778     293     190
 
Bsp1286I G_dGCh'C

Cuts at:    445     698     991    1602    1695    1769    1959    2021    2751 
   Size:        253     293     611      93      74     190      62     730

Cuts at:   2751     445 
   Size:       1671

  Fragments arranged by size: 

                1671     730     611     293     253     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3197    3197 
   Size:       3977
 
BspHI T'CATG_A

Cuts at:   3153    3153 
   Size:       3977
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    842    1027    1241    1543    1924     842 
   Size:        185     214     302     381    2895

  Fragments arranged by size: 

                2895     381     302     214     185
 
BsrBI CCG'CTC

Cuts at:     50    2269    2323    3807    3972      50 
   Size:       2219      54    1484     165      55

  Fragments arranged by size: 

                2219    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1889    1889 
   Size:       3977
 
BsrFI r'CCGG_y

Cuts at:   1975    2156    2365    1975 
   Size:        181     209    3587

  Fragments arranged by size: 

                3587     209     181
 
BssSI C'ACGA_G

Cuts at:   2248    2606    2248 
   Size:        358    3619
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1354    1354 
   Size:       3977
 
BstBI TT'CG_AA

Cuts at:   2338    2338 
   Size:       3977
 
BstYI r'GATC_y

Cuts at:      1     681    1002    1469    1492    1827    2073    3085    3171 
   Size:        680     321     467      23     335     246    1012      86

Cuts at:   3171    3183    3529       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     680     467     449     346     335     321     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3977
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1927    2108    2271    1927 
   Size:        181     163    3633

  Fragments arranged by size: 

                3633     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1220    1220 
   Size:       3977
 
DraI TTT'AAA

Cuts at:    976     976 
   Size:       3977
 
DrdI GACnn_nn'nnGTC

Cuts at:    336    1683    2541     336 
   Size:       1347     858    1772

  Fragments arranged by size: 

                1772    1347     858
 
EaeI y'GGCC_r

Cuts at:   1562    1736    2127    2154    3499    1562 
   Size:        174     391      27    1345    2040

  Fragments arranged by size: 

                2040    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1562    1562 
   Size:       3977
 
EarI CTCTTCn'nnn_

Cuts at:    272    2000    2210     272 
   Size:       1728     210    2039

  Fragments arranged by size: 

                2039    1728     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2185    2495    2641    3787    2185 
   Size:        310     146    1146    2375

  Fragments arranged by size: 

                2375    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    696     989     696 
   Size:        293    3684
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    484     524     623    1802    2234    2981    3249     484 
   Size:         40      99    1179     432     747     268    1212

  Fragments arranged by size: 

                1212    1179     747     432     268      99      40
 
EcoO109I rG'GnC_Cy

Cuts at:   1116    1116 
   Size:       3977
 
EcoRI G'AATT_C

Cuts at:    981     981 
   Size:       3977
 
EcoRV GAT'ATC

Cuts at:    390     390 
   Size:       3977
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1996    1996 
   Size:       3977
 
FspI TGC'GCA

Cuts at:   1758    1758 
   Size:       3977
 
HaeII r_GCGC'y

Cuts at:   1411    1659    2681    3660    1411 
   Size:        248    1022     979    1728

  Fragments arranged by size: 

                1728    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    405     437    1819    1849    1851    1881     405 
   Size:         32    1382      30       2      30    2501

  Fragments arranged by size: 

                2501    1382      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1016    1016 
   Size:       3977
 
KasI G'GCGC_C

Cuts at:   1407    1655    1407 
   Size:        248    3729
 
KpnI G_GTAC'C

Cuts at:    997     997 
   Size:       3977
 
MluI A'CGCG_T

Cuts at:   3410    3410 
   Size:       3977
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    589     614    2648    2832     589 
   Size:         25    2034     184    1734

  Fragments arranged by size: 

                2034    1734     184      25
 
MscI TGG'CCA

Cuts at:   1738    1738 
   Size:       3977
 
MslI CAynn'nnrTG

Cuts at:    144     450    1966    2093     144 
   Size:        306    1516     127    2028

  Fragments arranged by size: 

                2028    1516     306     127
 
MspA1I CmG'CkG

Cuts at:   1088    1402    1762    2775    3020    1088 
   Size:        314     360    1013     245    2045

  Fragments arranged by size: 

                2045    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2158    2158 
   Size:       3977
 
NarI GG'CG_CC

Cuts at:   1408    1656    1408 
   Size:        248    3729
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3977
 
NgoMIV G'CCGG_C

Cuts at:   2156    2156 
   Size:       3977
 
NspI r_CATG'y

Cuts at:   1030    1530    2437    1030 
   Size:        500     907    2570

  Fragments arranged by size: 

                2570     907     500
 
PciI A'CATG_T

Cuts at:   2433    2433 
   Size:       3977
 
PmeI GTTT'AAAC

Cuts at:    976     976 
   Size:       3977
 
PmlI CAC'GTG

Cuts at:    219     219 
   Size:       3977
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3116    3148    3116 
   Size:         32    3945
 
PstI C_TGCA'G

Cuts at:   1024    1709    1024 
   Size:        685    3292
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3977
 
PvuII CAG'CTG

Cuts at:   1402    1762    1402 
   Size:        360    3617
 
RsrII CG'GwC_CG

Cuts at:   2172    2172 
   Size:       3977
 
SacI G_AGCT'C

Cuts at:    698     991     698 
   Size:        293    3684
 
SalI G'TCGA_C

Cuts at:   1014    1014 
   Size:       3977
 
SapI GCTCTTCn'nnn_

Cuts at:    272    2000    2210     272 
   Size:       1728     210    2039

  Fragments arranged by size: 

                2039    1728     210
 
SbfI CC_TGCA'GG

Cuts at:   1024    1024 
   Size:       3977
 
ScaI AGT'ACT

Cuts at:   3494    3494 
   Size:       3977
 
SfcI C'TryA_G

Cuts at:     33     968    1020    1705    2698    2889      33 
   Size:        935      52     685     993     191    1121

  Fragments arranged by size: 

                1121     993     935     685     191      52
 
SfoI GGC'GCC

Cuts at:   1409    1657    1409 
   Size:        248    3729
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3977
 
SmaI CCC'GGG

Cuts at:    999     999 
   Size:       3977
 
SmlI C'TyrA_G

Cuts at:   1132    2047    2539    2801    1132 
   Size:        915     492     262    2308

  Fragments arranged by size: 

                2308     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3977
 
SphI G_CATG'C

Cuts at:   1030    1530    1030 
   Size:        500    3477
 
SspI AAT'ATT

Cuts at:    576     576 
   Size:       3977
 
StyI C'CwwG_G

Cuts at:    107     224    1111    1465     107 
   Size:        117     887     354    2619

  Fragments arranged by size: 

                2619     887     354     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1560    1693    2152    2361    1560 
   Size:        133     459     209    3176

  Fragments arranged by size: 

                3176     459     209     133
 
TatI w'GTAC_w

Cuts at:    365    3492     365 
   Size:       3127     850
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    866     898    3494    3526     866 
   Size:         32    2596      32    1317

  Fragments arranged by size: 

                2596    1317      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1774    1774 
   Size:       3977
 
XbaI T'CTAG_A

Cuts at:   1008    1008 
   Size:       3977
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    414    1472     414 
   Size:       1058    2919
 
XmaI C'CCGG_G

Cuts at:    997     997 
   Size:       3977
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PmeI     PmlI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     XmnI     ZraI