Restriction map for
pF1KE1780
(Circular) (Six-base) MAPSORT of: pF1KE1780.seq Check: 3765 from: 1 to: 3977
pF1KE1780 3977 bp
With 182 enzymes: SgfI *
June 10, 2016 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 274 274
Size: 3977
AccI GT'mk_AC
Cuts at: 1015 1015
Size: 3977
Acc65I G'GTAC_C
Cuts at: 308 993 308
Size: 685 3292
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1802 2234 2981 3249 754
Size: 1048 432 747 268 1482
Fragments arranged by size:
1482 1048 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 200 732 936 1282 2849 200
Size: 532 204 346 1567 1328
Fragments arranged by size:
1567 1328 532 346 204
ApaI G_GGCC'C
Cuts at: 447 447
Size: 3977
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 355 686 997 355
Size: 331 311 3335
Fragments arranged by size:
3335 331 311
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 123 308 463 880 993 1407 1655 1690 123
Size: 185 155 417 113 414 248 35 2410
Fragments arranged by size:
2410 417 414 248 185 155 113 35
BanII G_rGCy'C
Cuts at: 322 447 533 818 907 991 2021 322
Size: 125 86 285 89 84 1030 2278
Fragments arranged by size:
2278 1030 285 125 89 86 84
BbeI G_GCGC'C
Cuts at: 127 1411 1659 127
Size: 1284 248 2445
Fragments arranged by size:
2445 1284 248
BbsI GAAGACnn'nnnn_
Cuts at: 811 811
Size: 3977
BbvCI CC'TCA_GC
Cuts at: 747 747
Size: 3977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3943
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 1497 1497
Size: 3977
BglI GCCn_nnn'nGGC
Cuts at: 374 414 499 641 939 374
Size: 40 85 142 298 3412
Fragments arranged by size:
3412 298 142 85 40
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 906 1089 906
Size: 183 3794
Bme1580I G_kGCm'C
Cuts at: 447 563 827 883 930 1602 1695 2751 447
Size: 116 264 56 47 672 93 1056 1673
Fragments arranged by size:
1673 1056 672 264 116 93 56 47
BmrI ACTGGGnnnn_n'
Cuts at: 166 1189 1270 1605 3749 166
Size: 1023 81 335 2144 394
Fragments arranged by size:
2144 1023 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 762 2344 762
Size: 1582 2395
Bpu10I CC'TnA_GC
Cuts at: 532 747 836 532
Size: 215 89 3673
Fragments arranged by size:
3673 215 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 124 1408 1656 2358 3854 124
Size: 1284 248 702 1496 247
Fragments arranged by size:
1496 1284 702 248 247
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 222 322 574 639 929 1402 2160 2737 3233
Size: 100 252 65 290 473 758 577 496
Cuts at: 3233 222
Size: 966
Fragments arranged by size:
966 758 577 496 473 290 252 100
65
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 533 991 1769 1959 2751 533
Size: 458 778 190 792 1759
Fragments arranged by size:
1759 792 778 458 190
Bsp1286I G_dGCh'C
Cuts at: 322 447 533 563 818 827 883 907 930
Size: 125 86 30 255 9 56 24 23
Cuts at: 930 991 1602 1695 1769 1959 2021 2751 322
Size: 61 611 93 74 190 62 730 1548
Fragments arranged by size:
1548 730 611 255 190 125 93 86
74 62 61 56 30 24 23 9
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 274 1027 1241 1543 1924 274
Size: 753 214 302 381 2327
Fragments arranged by size:
2327 753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2269 2323 3807 3972 50
Size: 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 459 1889 459
Size: 1430 2547
BsrFI r'CCGG_y
Cuts at: 595 721 1975 2156 2365 595
Size: 126 1254 181 209 2207
Fragments arranged by size:
2207 1254 209 181 126
BssSI C'ACGA_G
Cuts at: 232 695 2248 2606 232
Size: 463 1553 358 1603
Fragments arranged by size:
1603 1553 463 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstEII G'GTnAC_C
Cuts at: 237 237
Size: 3977
BstXI CCAn_nnnn'nTGG
Cuts at: 140 489 140
Size: 349 3628
BstYI r'GATC_y
Cuts at: 1 113 1002 1469 1492 1827 2073 3085 3171
Size: 112 889 467 23 335 246 1012 86
Cuts at: 3171 3183 3529 1
Size: 12 346 449
Fragments arranged by size:
1012 889 467 449 346 335 246 112
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 944 944
Size: 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 954 1220 954
Size: 266 3711
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 130 166 344 375 597 671 723 1562 1736
Size: 36 178 31 222 74 52 839 174
Cuts at: 1736 2127 2154 3499 130
Size: 391 27 1345 608
Fragments arranged by size:
1345 839 608 391 222 178 174 74
52 36 31 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 531 989 531
Size: 458 3519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1802 2234 2981 3249 754
Size: 1048 432 747 268 1482
Fragments arranged by size:
1482 1048 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 585 736 585
Size: 151 3826
EcoO109I rG'GnC_Cy
Cuts at: 197 443 666 741 1116 197
Size: 246 223 75 375 3058
Fragments arranged by size:
3058 375 246 223 75
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 127 1411 1659 2681 3660 127
Size: 1284 248 1022 979 444
Fragments arranged by size:
1284 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1819 1849 1851 1881 1819
Size: 30 2 30 3915
Fragments arranged by size:
3915 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 123 1407 1655 123
Size: 1284 248 2445
Fragments arranged by size:
2445 1284 248
KpnI G_GTAC'C
Cuts at: 312 997 312
Size: 685 3292
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 2648 2832 302
Size: 2346 184 1447
Fragments arranged by size:
2346 1447 184
MscI TGG'CCA
Cuts at: 132 1738 132
Size: 1606 2371
MslI CAynn'nnrTG
Cuts at: 433 1966 2093 433
Size: 1533 127 2317
Fragments arranged by size:
2317 1533 127
MspA1I CmG'CkG
Cuts at: 461 910 1088 1402 1762 2775 3020 461
Size: 449 178 314 360 1013 245 1418
Fragments arranged by size:
1418 1013 449 360 314 245 178
NaeI GCC'GGC
Cuts at: 597 2158 597
Size: 1561 2416
NarI GG'CG_CC
Cuts at: 124 1408 1656 124
Size: 1284 248 2445
Fragments arranged by size:
2445 1284 248
NgoMIV G'CCGG_C
Cuts at: 595 2156 595
Size: 1561 2416
NspI r_CATG'y
Cuts at: 1030 1530 2437 1030
Size: 500 907 2570
Fragments arranged by size:
2570 907 500
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 612 612
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PpuMI rG'GwC_Cy
Cuts at: 666 741 666
Size: 75 3902
PspOMI G'GGCC_C
Cuts at: 443 443
Size: 3977
PstI C_TGCA'G
Cuts at: 271 1024 1709 271
Size: 753 685 2539
Fragments arranged by size:
2539 753 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 910 1402 1762 910
Size: 492 360 3125
Fragments arranged by size:
3125 492 360
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 533 991 533
Size: 458 3519
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 267 1020 1705 2698 2889 33
Size: 234 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 234 191
SfoI GGC'GCC
Cuts at: 125 1409 1657 125
Size: 1284 248 2445
Fragments arranged by size:
2445 1284 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 688 999 688
Size: 311 3666
SmlI C'TyrA_G
Cuts at: 1132 2047 2539 2801 1132
Size: 915 492 262 2308
Fragments arranged by size:
2308 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StuI AGG'CCT
Cuts at: 942 942
Size: 3977
StyI C'CwwG_G
Cuts at: 497 1111 1465 497
Size: 614 354 3009
Fragments arranged by size:
3009 614 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 600 1560 1693 2152 2361 256
Size: 344 960 133 459 209 1872
Fragments arranged by size:
1872 960 459 344 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 778 810 3494 3526 778
Size: 32 2684 32 1229
Fragments arranged by size:
2684 1229 32 32
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 125 197 1472 125
Size: 72 1275 2630
Fragments arranged by size:
2630 1275 72
XmaI C'CCGG_G
Cuts at: 686 997 686
Size: 311 3666
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BpmI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI