Restriction map for
pF1KE1824
(Circular) (Six-base) MAPSORT of: pF1KE1824.seq Check: 6658 from: 1 to: 4136
pF1KE1824 4136 bp
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 1174 1174
Size: 4136
Acc65I G'GTAC_C
Cuts at: 645 1152 645
Size: 507 3629
AclI AA'CG_TT
Cuts at: 1130 3976 1130
Size: 2846 1290
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1961 2393 3140 3408 694
Size: 1267 432 747 268 1422
Fragments arranged by size:
1422 1267 747 432 268
AflIII A'CryG_T
Cuts at: 272 561 1488 2592 3569 272
Size: 289 927 1104 977 839
Fragments arranged by size:
1104 977 927 839 289
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 329 361 329
Size: 32 4104
AlwNI CAG_nnn'CTG
Cuts at: 1441 3008 1441
Size: 1567 2569
ApaI G_GGCC'C
Cuts at: 944 944
Size: 4136
ApaLI G'TGCA_C
Cuts at: 2906 2906
Size: 4136
ApoI r'AATT_y
Cuts at: 1140 3489 3745 1140
Size: 2349 256 1531
Fragments arranged by size:
2349 1531 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 229 1156 229
Size: 927 3209
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 645 712 990 1152 1566 1814 1849 645
Size: 67 278 162 414 248 35 2932
Fragments arranged by size:
2932 414 278 248 162 67 35
BanII G_rGCy'C
Cuts at: 944 1150 2180 944
Size: 206 1030 2900
Fragments arranged by size:
2900 1030 206
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BbsI GAAGACnn'nnnn_
Cuts at: 671 671
Size: 4136
BciVI GTATCCnnnnn_n'
Cuts at: 1025 2034 2795 4136 1025
Size: 1009 761 1341 1025
Fragments arranged by size:
1341 1025 1009 761
BclI T'GATC_A
Cuts at: 869 1656 869
Size: 787 3349
BglII A'GATC_T
Cuts at: 1651 3688 1651
Size: 2037 2099
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 944 1761 1854 2910 944
Size: 817 93 1056 2170
Fragments arranged by size:
2170 1056 817 93
BmrI ACTGGGnnnn_n'
Cuts at: 806 1348 1429 1764 3908 806
Size: 542 81 335 2144 1034
Fragments arranged by size:
2144 1034 542 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 4136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 920
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1312 2191 2683 2981 1030
Size: 282 879 492 298 2185
Fragments arranged by size:
2185 879 492 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 1650 3687 6
Size: 1644 2037 455
Fragments arranged by size:
2037 1644 455
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 457 1325 1846 2524 2798 2945 3356 457
Size: 868 521 678 274 147 411 1237
Fragments arranged by size:
1237 868 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 526 899 526
Size: 373 3763
BseYI C'CCAG_C
Cuts at: 1561 2319 2896 3392 1561
Size: 758 577 496 2305
Fragments arranged by size:
2305 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 4136
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1150 1928 2118 2910 1150
Size: 778 190 792 2376
Fragments arranged by size:
2376 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 944 1150 1761 1854 1928 2118 2180 2910 944
Size: 206 611 93 74 190 62 730 2170
Fragments arranged by size:
2170 730 611 206 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3356 3356
Size: 4136
BspHI T'CATG_A
Cuts at: 188 3312 188
Size: 3124 1012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 657 1186 1400 1702 2083 657
Size: 529 214 302 381 2710
Fragments arranged by size:
2710 529 381 302 214
BsrBI CCG'CTC
Cuts at: 50 234 2428 2482 3966 4131 50
Size: 184 2194 54 1484 165 55
Fragments arranged by size:
2194 1484 184 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 807 1123 2134 2315 2524 807
Size: 316 1011 181 209 2419
Fragments arranged by size:
2419 1011 316 209 181
BsrGI T'GTAC_A
Cuts at: 275 275
Size: 4136
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 1074 1513 1074
Size: 439 3697
BstBI TT'CG_AA
Cuts at: 1062 2497 1062
Size: 1435 2701
BstYI r'GATC_y
Cuts at: 1 215 1161 1628 1651 1986 2232 3244 3330
Size: 214 946 467 23 335 246 1012 86
Cuts at: 3330 3342 3688 1
Size: 12 346 449
Fragments arranged by size:
1012 946 467 449 346 335 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1379
Size: 4136
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 1721 1895 2286 2313 3658 1721
Size: 174 391 27 1345 2199
Fragments arranged by size:
2199 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 607 2159 2369 607
Size: 1552 210 2374
Fragments arranged by size:
2374 1552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 2654 2800 3946 2344
Size: 310 146 1146 2534
Fragments arranged by size:
2534 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 920 1961 2393 3140 3408 694
Size: 226 1041 432 747 268 1422
Fragments arranged by size:
1422 1041 747 432 268 226
EcoO109I rG'GnC_Cy
Cuts at: 337 940 1275 337
Size: 603 335 3198
Fragments arranged by size:
3198 603 335
EcoRI G'AATT_C
Cuts at: 1140 1140
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 379 2155 379
Size: 1776 2360
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1570 1818 2840 3819 1570
Size: 248 1022 979 1887
Fragments arranged by size:
1887 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 452 484 587 619 1978 2008 2010 2040 452
Size: 32 103 32 1359 30 2 30 2548
Fragments arranged by size:
2548 1359 103 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 649 1156 649
Size: 507 3629
MfeI C'AATT_G
Cuts at: 534 534
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2991 2807
Size: 184 3952
MscI TGG'CCA
Cuts at: 1897 1897
Size: 4136
MslI CAynn'nnrTG
Cuts at: 548 2125 2252 548
Size: 1577 127 2432
Fragments arranged by size:
2432 1577 127
MspA1I CmG'CkG
Cuts at: 168 687 823 847 889 1247 1561 1921 2934
Size: 519 136 24 42 358 314 360 1013
Cuts at: 2934 3179 168
Size: 245 1125
Fragments arranged by size:
1125 1013 519 360 358 314 245 136
42 24
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 107 107
Size: 4136
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 276 565 1189 1689 2596 276
Size: 289 624 500 907 1816
Fragments arranged by size:
1816 907 624 500 289
PciI A'CATG_T
Cuts at: 272 561 2592 272
Size: 289 2031 1816
Fragments arranged by size:
2031 1816 289
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 4104
PpuMI rG'GwC_Cy
Cuts at: 337 337
Size: 4136
PshAI GACnn'nnGTC
Cuts at: 889 889
Size: 4136
PspOMI G'GGCC_C
Cuts at: 940 940
Size: 4136
PspXI vC'TCGA_Gb
Cuts at: 229 229
Size: 4136
PstI C_TGCA'G
Cuts at: 654 1183 1868 654
Size: 529 685 2922
Fragments arranged by size:
2922 685 529
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 168 687 823 847 1561 1921 168
Size: 519 136 24 714 360 2383
Fragments arranged by size:
2383 714 519 360 136 24
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SfcI C'TryA_G
Cuts at: 33 650 717 1179 1864 2857 3048 33
Size: 617 67 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 617 462 191 67
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 229 1009 1291 2206 2698 2960 229
Size: 780 282 915 492 262 1405
Fragments arranged by size:
1405 915 780 492 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 1189 1689 1189
Size: 500 3636
StyI C'CwwG_G
Cuts at: 107 414 784 1270 1624 107
Size: 307 370 486 354 2619
Fragments arranged by size:
2619 486 370 354 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 929 1127 1719 1852 2311 2520 929
Size: 198 592 133 459 209 2545
Fragments arranged by size:
2545 592 459 209 198 133
TatI w'GTAC_w
Cuts at: 275 298 564 3651 275
Size: 23 266 3087 760
Fragments arranged by size:
3087 760 266 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1631 1631
Size: 4136
XhoI C'TCGA_G
Cuts at: 229 229
Size: 4136
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PsiI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI