Restriction map for
pF1KE1843
(Circular) (Six-base) MAPSORT of: pF1KE1843.seq Check: 9082 from: 1 to: 4187
pF1KE1843 4187 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 617 617
Size: 4187
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 2012 2444 3191 3459 179
Size: 1833 432 747 268 907
Fragments arranged by size:
1833 907 747 432 268
AfeI AGC'GCT
Cuts at: 201 201
Size: 4187
AflIII A'CryG_T
Cuts at: 1091 1539 2643 3620 1091
Size: 448 1104 977 1658
Fragments arranged by size:
1658 1104 977 448
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1031 1063 1031
Size: 32 4155
AlwNI CAG_nnn'CTG
Cuts at: 707 1492 3059 707
Size: 785 1567 1835
Fragments arranged by size:
1835 1567 785
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 1207 1207
Size: 4187
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 1203 1617 1865 1900 1203
Size: 414 248 35 3490
Fragments arranged by size:
3490 414 248 35
BanII G_rGCy'C
Cuts at: 414 748 1201 2231 414
Size: 334 453 1030 2370
Fragments arranged by size:
2370 1030 453 334
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BbsI GAAGACnn'nnnn_
Cuts at: 401 401
Size: 4187
BbvCI CC'TCA_GC
Cuts at: 196 196
Size: 4187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4153
BciVI GTATCCnnnnn_n'
Cuts at: 436 2085 2846 4187 436
Size: 1649 761 1341 436
Fragments arranged by size:
1649 1341 761 436
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4187
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 1812 1905 2961 1812
Size: 93 1056 3038
Fragments arranged by size:
3038 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 717 837 1399 1480 1815 3959 717
Size: 120 562 81 335 2144 945
Fragments arranged by size:
2144 945 562 335 120 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 986 2554 986
Size: 1568 2619
Bpu10I CC'TnA_GC
Cuts at: 196 196
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 1363 2242 2734 3032 398
Size: 965 879 492 298 1553
Fragments arranged by size:
1553 965 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 215 526 3891 215
Size: 311 3365 511
Fragments arranged by size:
3365 511 311
BsaAI yAC'GTr
Cuts at: 1540 1540
Size: 4187
BsaBI GATnn'nnATC
Cuts at: 6 317 1701 3738 6
Size: 311 1384 2037 455
Fragments arranged by size:
2037 1384 455 311
BsaHI Gr'CG_yC
Cuts at: 1618 1866 2568 4064 1618
Size: 248 702 1496 1741
Fragments arranged by size:
1741 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 790 910 145
Size: 645 120 3422
Fragments arranged by size:
3422 645 120
BseYI C'CCAG_C
Cuts at: 1612 2370 2947 3443 1612
Size: 758 577 496 2356
Fragments arranged by size:
2356 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 867 867
Size: 4187
BsiEI CG_ry'CG
Cuts at: 104 1729 1775 2983 104
Size: 1625 46 1208 1308
Fragments arranged by size:
1625 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 414 748 1201 1979 2169 2961 414
Size: 334 453 778 190 792 1640
Fragments arranged by size:
1640 792 778 453 334 190
Bsp1286I G_dGCh'C
Cuts at: 414 748 1201 1812 1905 1979 2169 2231 2961
Size: 334 453 611 93 74 190 62 730
Cuts at: 2961 414
Size: 1640
Fragments arranged by size:
1640 730 611 453 334 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 617 1237 1451 1753 2134 617
Size: 620 214 302 381 2670
Fragments arranged by size:
2670 620 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 2185 2366 2575 2185
Size: 181 209 3797
Fragments arranged by size:
3797 209 181
BssSI C'ACGA_G
Cuts at: 2458 2816 2458
Size: 358 3829
BstAPI GCAn_nnn'nTGC
Cuts at: 1564 1564
Size: 4187
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstEII G'GTnAC_C
Cuts at: 1024 1057 1024
Size: 33 4154
BstYI r'GATC_y
Cuts at: 1 134 738 1212 1679 1702 2037 2283 3295
Size: 133 604 474 467 23 335 246 1012
Cuts at: 3295 3381 3393 3739 1
Size: 86 12 346 449
Fragments arranged by size:
1012 604 474 467 449 346 335 246
133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 128 128
Size: 4187
BtgI C'CryG_G
Cuts at: 1020 1020
Size: 4187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 513 2137 2318 2481 513
Size: 1624 181 163 2219
Fragments arranged by size:
2219 1624 181 163
BtsI GCAGTG_nn'
Cuts at: 1157 1430 1157
Size: 273 3914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1146 1181 1146
Size: 35 4152
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 1772 1946 2337 2364 3709 1772
Size: 174 391 27 1345 2250
Fragments arranged by size:
2250 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 155 529 541 609 761 788 932 947 2210
Size: 374 12 68 152 27 144 15 1263
Cuts at: 2210 2420 155
Size: 210 1922
Fragments arranged by size:
1922 1263 374 210 152 144 68 27
15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 2705 2851 3997 2395
Size: 310 146 1146 2585
Fragments arranged by size:
2585 1146 310 146
EcoICRI GAG'CTC
Cuts at: 412 746 1199 412
Size: 334 453 3400
Fragments arranged by size:
3400 453 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 2012 2444 3191 3459 179
Size: 1833 432 747 268 907
Fragments arranged by size:
1833 907 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 127 577 127
Size: 450 3737
EcoO109I rG'GnC_Cy
Cuts at: 566 1005 1326 566
Size: 439 321 3427
Fragments arranged by size:
3427 439 321
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 203 1621 1869 2891 3870 203
Size: 1418 248 1022 979 520
Fragments arranged by size:
1418 1022 979 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 842 874 1027 1059 2029 2059 2061
Size: 32 80 32 153 32 970 30 2
Cuts at: 2061 2091 730
Size: 30 2826
Fragments arranged by size:
2826 970 153 80 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1226 1226
Size: 4187
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1074 2858 3042 1074
Size: 1784 184 2219
Fragments arranged by size:
2219 1784 184
MscI TGG'CCA
Cuts at: 1948 1948
Size: 4187
MslI CAynn'nnrTG
Cuts at: 2176 2303 2176
Size: 127 4060
MspA1I CmG'CkG
Cuts at: 326 1298 1612 1972 2985 3230 326
Size: 972 314 360 1013 245 1283
Fragments arranged by size:
1283 1013 972 360 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 1020 1020
Size: 4187
NdeI CA'TA_TG
Cuts at: 762 801 762
Size: 39 4148
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NspI r_CATG'y
Cuts at: 1095 1240 1740 2647 1095
Size: 145 500 907 2635
Fragments arranged by size:
2635 907 500 145
PciI A'CATG_T
Cuts at: 1091 2643 1091
Size: 1552 2635
PflMI CCAn_nnn'nTGG
Cuts at: 707 835 707
Size: 128 4059
PfoI T'CCnGG_A
Cuts at: 146 146
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PpuMI rG'GwC_Cy
Cuts at: 566 566
Size: 4187
PstI C_TGCA'G
Cuts at: 1234 1919 1234
Size: 685 3502
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 1612 1972 1612
Size: 360 3827
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 414 748 1201 414
Size: 334 453 3400
Fragments arranged by size:
3400 453 334
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 541 932 2210 2420 541
Size: 391 1278 210 2308
Fragments arranged by size:
2308 1278 391 210
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 890 1230 1915 2908 3099 33
Size: 857 340 685 993 191 1121
Fragments arranged by size:
1121 993 857 685 340 191
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 413 1342 2257 2749 3011 413
Size: 929 915 492 262 1589
Fragments arranged by size:
1589 929 915 492 262
SpeI A'CTAG_T
Cuts at: 67 685 67
Size: 618 3569
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StyI C'CwwG_G
Cuts at: 282 1020 1321 1675 282
Size: 738 301 354 2794
Fragments arranged by size:
2794 738 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 699 1095 1770 1903 2362 2571 699
Size: 396 675 133 459 209 2315
Fragments arranged by size:
2315 675 459 396 209 133
TatI w'GTAC_w
Cuts at: 3702 3702
Size: 4187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4155
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 948 1218 948
Size: 270 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 1682 708
Size: 974 3213
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
XmnI GAAnn'nnTTC
Cuts at: 164 618 164
Size: 454 3733
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BpmI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI