Restriction map for
pF1KE1861
(Circular) (Six-base) MAPSORT of: pF1KE1861.seq Check: 2518 from: 1 to: 4253
pF1KE1861 4253 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 598 598
Size: 4253
AatII G_ACGT'C
Cuts at: 1093 1093
Size: 4253
AccI GT'mk_AC
Cuts at: 1007 1291 1007
Size: 284 3969
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 925 2078 2510 3257 3525 874
Size: 51 1153 432 747 268 1602
Fragments arranged by size:
1602 1153 747 432 268 51
AfeI AGC'GCT
Cuts at: 1168 1168
Size: 4253
AflIII A'CryG_T
Cuts at: 1605 2709 3686 1605
Size: 1104 977 2172
Fragments arranged by size:
2172 1104 977
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 4253
AlwNI CAG_nnn'CTG
Cuts at: 1558 3125 1558
Size: 1567 2686
ApaLI G'TGCA_C
Cuts at: 1215 3023 1215
Size: 1808 2445
ApoI r'AATT_y
Cuts at: 1257 3606 3862 1257
Size: 2349 256 1648
Fragments arranged by size:
2349 1648 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 1273 1273
Size: 4253
BamHI G'GATC_C
Cuts at: 1278 1278
Size: 4253
BanI G'GyrC_C
Cuts at: 1269 1683 1931 1966 1269
Size: 414 248 35 3556
Fragments arranged by size:
3556 414 248 35
BanII G_rGCy'C
Cuts at: 736 1118 1267 2297 736
Size: 382 149 1030 2692
Fragments arranged by size:
2692 1030 382 149
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbsI GAAGACnn'nnnn_
Cuts at: 164 406 862 164
Size: 242 456 3555
Fragments arranged by size:
3555 456 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1206 1240 1206
Size: 34 4219
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglI GCCn_nnn'nGGC
Cuts at: 575 575
Size: 4253
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 1365 1365
Size: 4253
Bme1580I G_kGCm'C
Cuts at: 1066 1219 1878 1971 3027 1066
Size: 153 659 93 1056 2292
Fragments arranged by size:
2292 1056 659 153 93
BmrI ACTGGGnnnn_n'
Cuts at: 1465 1546 1881 4025 1465
Size: 81 335 2144 1693
Fragments arranged by size:
2144 1693 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 1429 2308 2800 3098 320
Size: 1109 879 492 298 1475
Fragments arranged by size:
1475 1109 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 1606 1606
Size: 4253
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 701 1090 1684 1932 2634 4130 701
Size: 389 594 248 702 1496 824
Fragments arranged by size:
1496 824 702 594 389 248
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 2915 3062 3473 1442
Size: 521 678 274 147 411 2222
Fragments arranged by size:
2222 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1018 1059 1678 2436 3013 3509 1018
Size: 41 619 758 577 496 1762
Fragments arranged by size:
1762 758 619 577 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1177 751
Size: 426 3827
BsiEI CG_ry'CG
Cuts at: 104 786 1131 1795 1841 3049 104
Size: 682 345 664 46 1208 1308
Fragments arranged by size:
1308 1208 682 664 345 46
BsiHKAI G_wGCw'C
Cuts at: 303 750 1051 1118 1219 1267 2045 2235 3027
Size: 447 301 67 101 48 778 190 792
Cuts at: 3027 303
Size: 1529
Fragments arranged by size:
1529 792 778 447 301 190 101 67
48
BsmI GAATG_Cn'
Cuts at: 421 421
Size: 4253
BsmBI CGTCTCn'nnnn_
Cuts at: 502 502
Size: 4253
Bsp1286I G_dGCh'C
Cuts at: 303 736 750 1051 1066 1118 1219 1267 1878
Size: 433 14 301 15 52 101 48 611
Cuts at: 1878 1971 2045 2235 2297 3027 303
Size: 93 74 190 62 730 1529
Fragments arranged by size:
1529 730 611 433 301 190 101 93
74 62 52 48 15 14
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 758 3429 758
Size: 2671 1582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 598 632 773 1303 1517 1819 2200 598
Size: 34 141 530 214 302 381 2651
Fragments arranged by size:
2651 530 381 302 214 141 34
BsrBI CCG'CTC
Cuts at: 50 711 2545 2599 4083 4248 50
Size: 661 1834 54 1484 165 55
Fragments arranged by size:
1834 1484 661 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 1047 2165 173
Size: 874 1118 2261
Fragments arranged by size:
2261 1118 874
BsrFI r'CCGG_y
Cuts at: 781 1051 2251 2432 2641 781
Size: 270 1200 181 209 2393
Fragments arranged by size:
2393 1200 270 209 181
BsrGI T'GTAC_A
Cuts at: 797 797
Size: 4253
BssHII G'CGCG_C
Cuts at: 610 610
Size: 4253
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 1214 1630 1214
Size: 416 3837
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstYI r'GATC_y
Cuts at: 1 1278 1745 1768 2103 2349 3361 3447 3459
Size: 1277 467 23 335 246 1012 86 12
Cuts at: 3459 3805 1
Size: 346 449
Fragments arranged by size:
1277 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 628 828 1131 628
Size: 200 303 3750
Fragments arranged by size:
3750 303 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2203 2384 2547 2203
Size: 181 163 3909
Fragments arranged by size:
3909 181 163
BtsI GCAGTG_nn'
Cuts at: 1173 1496 1173
Size: 323 3930
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 2817 1959
Size: 858 3395
EaeI y'GGCC_r
Cuts at: 197 783 929 1128 1838 2012 2403 2430 3775
Size: 586 146 199 710 174 391 27 1345
Cuts at: 3775 197
Size: 675
Fragments arranged by size:
1345 710 675 586 391 199 174 146
27
EagI C'GGCC_G
Cuts at: 783 1128 1838 783
Size: 345 710 3198
Fragments arranged by size:
3198 710 345
EarI CTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1116 1265 1116
Size: 149 4104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 925 2078 2510 3257 3525 874
Size: 51 1153 432 747 268 1602
Fragments arranged by size:
1602 1153 747 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 621 1392 621
Size: 771 3482
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FseI GG_CCGG'CC
Cuts at: 785 785
Size: 4253
FspI TGC'GCA
Cuts at: 1039 2034 1039
Size: 995 3258
HaeII r_GCGC'y
Cuts at: 1170 1687 1935 2957 3936 1170
Size: 517 248 1022 979 1487
Fragments arranged by size:
1487 1022 979 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 2095 2125 2127 2157 454
Size: 32 1609 30 2 30 2550
Fragments arranged by size:
2550 1609 32 30 30 2
HincII GTy'rAC
Cuts at: 969 1292 969
Size: 323 3930
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 456 1073 2924 3108 456
Size: 617 1851 184 1601
Fragments arranged by size:
1851 1601 617 184
MscI TGG'CCA
Cuts at: 199 2014 199
Size: 1815 2438
MslI CAynn'nnrTG
Cuts at: 757 2242 2369 757
Size: 1485 127 2641
Fragments arranged by size:
2641 1485 127
MspA1I CmG'CkG
Cuts at: 630 873 1364 1678 2038 3051 3296 630
Size: 243 491 314 360 1013 245 1587
Fragments arranged by size:
1587 1013 491 360 314 245 243
NaeI GCC'GGC
Cuts at: 783 2434 783
Size: 1651 2602
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NcoI C'CATG_G
Cuts at: 828 828
Size: 4253
NgoMIV G'CCGG_C
Cuts at: 781 2432 781
Size: 1651 2602
NspI r_CATG'y
Cuts at: 378 863 1306 1806 2713 378
Size: 485 443 500 907 1918
Fragments arranged by size:
1918 907 500 485 443
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PflMI CCAn_nnn'nTGG
Cuts at: 242 242
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PpuMI rG'GwC_Cy
Cuts at: 621 621
Size: 4253
PstI C_TGCA'G
Cuts at: 316 454 601 872 1300 1985 316
Size: 138 147 271 428 685 2584
Fragments arranged by size:
2584 685 428 271 147 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 873 1678 2038 873
Size: 805 360 3088
Fragments arranged by size:
3088 805 360
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1118 1267 1118
Size: 149 4104
SacII CC_GC'GG
Cuts at: 631 631
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SexAI A'CCwGG_T
Cuts at: 1001 1001
Size: 4253
SfcI C'TryA_G
Cuts at: 33 312 450 597 868 1296 1981 2974 3165
Size: 279 138 147 271 428 685 993 191
Cuts at: 3165 33
Size: 1121
Fragments arranged by size:
1121 993 685 428 279 271 191 147
138
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 335 1408 2323 2815 3077 335
Size: 1073 915 492 262 1511
Fragments arranged by size:
1511 1073 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
SspI AAT'ATT
Cuts at: 128 128
Size: 4253
StyI C'CwwG_G
Cuts at: 617 686 728 828 1387 1741 617
Size: 69 42 100 559 354 3129
Fragments arranged by size:
3129 559 354 100 69 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 883 1836 1969 2428 2637 883
Size: 953 133 459 209 2499
Fragments arranged by size:
2499 953 459 209 133
TatI w'GTAC_w
Cuts at: 380 797 3768 380
Size: 417 2971 865
Fragments arranged by size:
2971 865 417
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4221
Tth111I GACn'n_nGTC
Cuts at: 1003 2050 1003
Size: 1047 3206
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 999 1748 693
Size: 306 749 3198
Fragments arranged by size:
3198 749 306
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
ZraI GAC'GTC
Cuts at: 1091 1091
Size: 4253
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI BseRI
BsiWI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI