Restriction map for
pF1KE1864
(Circular) (Six-base) MAPSORT of: pF1KE1864.seq Check: 6856 from: 1 to: 4268
pF1KE1864 4268 bp
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 958 958
Size: 4268
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 2093 2525 3272 3540 1000
Size: 1093 432 747 268 1728
Fragments arranged by size:
1728 1093 747 432 268
AflIII A'CryG_T
Cuts at: 1620 2724 3701 1620
Size: 1104 977 2187
Fragments arranged by size:
2187 1104 977
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AhdI GACnn_n'nnGTC
Cuts at: 824 824
Size: 4268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 928 960 928
Size: 32 4236
AlwNI CAG_nnn'CTG
Cuts at: 1158 1221 1573 3140 1158
Size: 63 352 1567 2286
Fragments arranged by size:
2286 1567 352 63
ApaI G_GGCC'C
Cuts at: 121 121
Size: 4268
ApaLI G'TGCA_C
Cuts at: 3038 3038
Size: 4268
ApoI r'AATT_y
Cuts at: 606 1272 3621 3877 606
Size: 666 2349 256 997
Fragments arranged by size:
2349 997 666 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 1288 1288
Size: 4268
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 1258 1284 1698 1946 1981 1258
Size: 26 414 248 35 3545
Fragments arranged by size:
3545 414 248 35 26
BanII G_rGCy'C
Cuts at: 121 182 496 524 582 1142 1282 2312 121
Size: 61 314 28 58 560 140 1030 2077
Fragments arranged by size:
2077 1030 560 314 140 61 58 28
BbeI G_GCGC'C
Cuts at: 1262 1702 1950 1262
Size: 440 248 3580
Fragments arranged by size:
3580 440 248
BbsI GAAGACnn'nnnn_
Cuts at: 117 181 482 637 117
Size: 64 301 155 3748
Fragments arranged by size:
3748 301 155 64
BbvCI CC'TCA_GC
Cuts at: 248 248
Size: 4268
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 1788 1788
Size: 4268
BfrBI ATG'CAT
Cuts at: 657 657
Size: 4268
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 176 1380 176
Size: 1204 3064
Bme1580I G_kGCm'C
Cuts at: 121 566 725 995 1893 1986 3042 121
Size: 445 159 270 898 93 1056 1347
Fragments arranged by size:
1347 1056 898 445 270 159 93
BmrI ACTGGGnnnn_n'
Cuts at: 561 1165 1480 1561 1896 4040 561
Size: 604 315 81 335 2144 789
Fragments arranged by size:
2144 789 604 335 315 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 558 430
Size: 128 4140
Bpu10I CC'TnA_GC
Cuts at: 248 248
Size: 4268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 2815 3113 1444
Size: 879 492 298 2599
Fragments arranged by size:
2599 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 355 3972 355
Size: 3617 651
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 825 1259 1699 1947 2649 4145 825
Size: 434 440 248 702 1496 948
Fragments arranged by size:
1496 948 702 440 434 248
BsaWI w'CCGG_w
Cuts at: 1457 1978 2656 2930 3077 3488 1457
Size: 521 678 274 147 411 2237
Fragments arranged by size:
2237 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 928 958 928
Size: 30 4238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 726 949 726
Size: 223 4045
BseYI C'CCAG_C
Cuts at: 114 497 857 1693 2451 3028 3524 114
Size: 383 360 836 758 577 496 858
Fragments arranged by size:
858 836 758 577 496 383 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 250
Size: 4268
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 496 524 582 1142 1282 2060 2250 3042 496
Size: 28 58 560 140 778 190 792 1722
Fragments arranged by size:
1722 792 778 560 190 140 58 28
BsmI GAATG_Cn'
Cuts at: 438 438
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 121 182 496 524 566 582 725 995 1142
Size: 61 314 28 42 16 143 270 147
Cuts at: 1142 1282 1893 1986 2060 2250 2312 3042 121
Size: 140 611 93 74 190 62 730 1347
Fragments arranged by size:
1347 730 611 314 270 190 147 143
140 93 74 62 61 42 28 16
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 958 1143 1318 1532 1834 2215 958
Size: 185 175 214 302 381 3011
Fragments arranged by size:
3011 381 302 214 185 175
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 2266 2447 2656 2266
Size: 181 209 3878
Fragments arranged by size:
3878 209 181
BssSI C'ACGA_G
Cuts at: 1141 2539 2897 1141
Size: 1398 358 2512
Fragments arranged by size:
2512 1398 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4268
BstYI r'GATC_y
Cuts at: 1 223 467 1293 1760 1783 2118 2364 3376
Size: 222 244 826 467 23 335 246 1012
Cuts at: 3376 3462 3474 3820 1
Size: 86 12 346 449
Fragments arranged by size:
1012 826 467 449 346 335 246 244
222 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 394 709 394
Size: 315 3953
BtgI C'CryG_G
Cuts at: 107 728 107
Size: 621 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 868 1511 868
Size: 643 3625
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DraIII CAC_nnn'GTG
Cuts at: 990 990
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 590 911 1853 2027 2418 2445 3790 590
Size: 321 942 174 391 27 1345 1068
Fragments arranged by size:
1345 1068 942 391 321 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 529 711 2291 2501 529
Size: 182 1580 210 2296
Fragments arranged by size:
2296 1580 210 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2476 2786 2932 4078 2476
Size: 310 146 1146 2666
Fragments arranged by size:
2666 1146 310 146
EcoICRI GAG'CTC
Cuts at: 494 522 580 1140 1280 494
Size: 28 58 560 140 3482
Fragments arranged by size:
3482 560 140 58 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 558 1000 2093 2525 3272 3540 430
Size: 128 442 1093 432 747 268 1158
Fragments arranged by size:
1158 1093 747 442 432 268 128
EcoO109I rG'GnC_Cy
Cuts at: 390 1155 1407 390
Size: 765 252 3251
Fragments arranged by size:
3251 765 252
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
EcoRV GAT'ATC
Cuts at: 688 688
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 4268
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 1262 1702 1950 2972 3951 1262
Size: 440 248 1022 979 1579
Fragments arranged by size:
1579 1022 979 440 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 393 425 630 662 810 823 842 855 1215
Size: 32 205 32 148 13 19 13 360
Cuts at: 1215 1247 2110 2140 2142 2172 393
Size: 32 863 30 2 30 2489
Fragments arranged by size:
2489 863 360 205 148 32 32 32
30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 1258 1698 1946 1258
Size: 440 248 3580
Fragments arranged by size:
3580 440 248
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3123 2939
Size: 184 4084
MscI TGG'CCA
Cuts at: 592 913 2029 592
Size: 321 1116 2831
Fragments arranged by size:
2831 1116 321
MslI CAynn'nnrTG
Cuts at: 260 772 1193 2257 2384 260
Size: 512 421 1064 127 2144
Fragments arranged by size:
2144 1064 512 421 127
MspA1I CmG'CkG
Cuts at: 968 1123 1379 1693 2053 3066 3311 968
Size: 155 256 314 360 1013 245 1925
Fragments arranged by size:
1925 1013 360 314 256 245 155
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 4268
NarI GG'CG_CC
Cuts at: 1259 1699 1947 1259
Size: 440 248 3580
Fragments arranged by size:
3580 440 248
NcoI C'CATG_G
Cuts at: 107 728 107
Size: 621 3647
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 4268
NsiI A_TGCA'T
Cuts at: 659 659
Size: 4268
NspI r_CATG'y
Cuts at: 661 782 1321 1821 2728 661
Size: 121 539 500 907 2201
Fragments arranged by size:
2201 907 539 500 121
PasI CC'CwG_GG
Cuts at: 312 312
Size: 4268
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 4268
PfoI T'CCnGG_A
Cuts at: 162 446 1157 162
Size: 284 711 3273
Fragments arranged by size:
3273 711 284
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 960 3407 3439 928
Size: 32 2447 32 1757
Fragments arranged by size:
2447 1757 32 32
PpuMI rG'GwC_Cy
Cuts at: 390 1155 390
Size: 765 3503
PsiI TTA'TAA
Cuts at: 197 197
Size: 4268
PspOMI G'GGCC_C
Cuts at: 117 117
Size: 4268
PstI C_TGCA'G
Cuts at: 674 1128 1154 1315 2000 674
Size: 454 26 161 685 2942
Fragments arranged by size:
2942 685 454 161 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 1123 1693 2053 1123
Size: 570 360 3338
Fragments arranged by size:
3338 570 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 496 524 582 1142 1282 496
Size: 28 58 560 140 3482
Fragments arranged by size:
3482 560 140 58 28
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 529 2291 2501 529
Size: 1762 210 2296
Fragments arranged by size:
2296 1762 210
SbfI CC_TGCA'GG
Cuts at: 674 1154 1315 674
Size: 480 161 3627
Fragments arranged by size:
3627 480 161
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 670 1124 1150 1311 1996 2989 3180 33
Size: 637 454 26 161 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 454 191 161 26
SfoI GGC'GCC
Cuts at: 1260 1700 1948 1260
Size: 440 248 3580
Fragments arranged by size:
3580 440 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 1423 2338 2830 3092 1423
Size: 915 492 262 2599
Fragments arranged by size:
2599 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 661 1321 1821 661
Size: 660 500 3108
Fragments arranged by size:
3108 660 500
StuI AGG'CCT
Cuts at: 754 754
Size: 4268
StyI C'CwwG_G
Cuts at: 107 587 728 927 1402 1756 107
Size: 480 141 199 475 354 2619
Fragments arranged by size:
2619 480 475 354 199 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 871 897 1184 1851 1984 2443 2652 871
Size: 26 287 667 133 459 209 2487
Fragments arranged by size:
2487 667 459 287 209 133 26
TatI w'GTAC_w
Cuts at: 3783 3783
Size: 4268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4236
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 690 1299 690
Size: 609 3659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1763 1763
Size: 4268
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI