Restriction map for
pF1KE1911
(Circular) (Six-base) MAPSORT of: pF1KE1911.seq Check: 1344 from: 1 to: 4448
pF1KE1911 4448 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 829
Size: 4448
AccI GT'mk_AC
Cuts at: 1486 1486
Size: 4448
Acc65I G'GTAC_C
Cuts at: 550 1464 550
Size: 914 3534
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 2273 2705 3452 3720 726
Size: 1547 432 747 268 1454
Fragments arranged by size:
1547 1454 747 432 268
AflIII A'CryG_T
Cuts at: 251 602 1800 2904 3881 251
Size: 351 1198 1104 977 818
Fragments arranged by size:
1198 1104 977 818 351
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AhdI GACnn_n'nnGTC
Cuts at: 1362 1362
Size: 4448
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 4448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1396 1428 1396
Size: 32 4416
AlwNI CAG_nnn'CTG
Cuts at: 1236 1753 3320 1236
Size: 517 1567 2364
Fragments arranged by size:
2364 1567 517
ApaI G_GGCC'C
Cuts at: 347 347
Size: 4448
ApaLI G'TGCA_C
Cuts at: 121 3218 121
Size: 3097 1351
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 339 967 1181 1468 339
Size: 628 214 287 3319
Fragments arranged by size:
3319 628 287 214
BamHI G'GATC_C
Cuts at: 1139 1473 1139
Size: 334 4114
BanI G'GyrC_C
Cuts at: 157 208 319 550 615 1464 1878 2126 2161
Size: 51 111 231 65 849 414 248 35
Cuts at: 2161 157
Size: 2444
Fragments arranged by size:
2444 849 414 248 231 111 65 51
35
BanII G_rGCy'C
Cuts at: 317 347 653 974 1462 2492 317
Size: 30 306 321 488 1030 2273
Fragments arranged by size:
2273 1030 488 321 306 30
BbeI G_GCGC'C
Cuts at: 161 1882 2130 161
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
BbvCI CC'TCA_GC
Cuts at: 1427 1427
Size: 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 169 135
Size: 34 4414
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 176 1968 176
Size: 1792 2656
BglI GCCn_nnn'nGGC
Cuts at: 351 351
Size: 4448
BglII A'GATC_T
Cuts at: 356 1963 4000 356
Size: 1607 2037 804
Fragments arranged by size:
2037 1607 804
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 125 211 290 347 422 968 2073 2166 3222
Size: 86 79 57 75 546 1105 93 1056
Cuts at: 3222 125
Size: 1351
Fragments arranged by size:
1351 1105 1056 546 93 86 79 75
57
BmrI ACTGGGnnnn_n'
Cuts at: 417 545 1660 1741 2076 4220 417
Size: 128 1115 81 335 2144 645
Fragments arranged by size:
2144 1115 645 335 128 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 4448
Bpu10I CC'TnA_GC
Cuts at: 1427 1427
Size: 4448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 2995 3293 1624
Size: 879 492 298 2779
Fragments arranged by size:
2779 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 694 4152 694
Size: 3458 990
BsaAI yAC'GTr
Cuts at: 285 1801 285
Size: 1516 2932
BsaBI GATnn'nnATC
Cuts at: 6 1962 3999 6
Size: 1956 2037 455
Fragments arranged by size:
2037 1956 455
BsaHI Gr'CG_yC
Cuts at: 158 1879 2127 2829 4325 158
Size: 1721 248 702 1496 281
Fragments arranged by size:
1721 1496 702 281 248
BsaWI w'CCGG_w
Cuts at: 1637 2158 2836 3110 3257 3668 1637
Size: 521 678 274 147 411 2417
Fragments arranged by size:
2417 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1396 1426 1396
Size: 30 4418
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 592 1414 1417 1432 1435 1444 1447 547
Size: 45 822 3 15 3 9 3 3548
Fragments arranged by size:
3548 822 45 15 9 3 3 3
BseYI C'CCAG_C
Cuts at: 397 977 1873 2631 3208 3704 397
Size: 580 896 758 577 496 1141
Fragments arranged by size:
1141 896 758 580 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1276 880
Size: 396 4052
BsiEI CG_ry'CG
Cuts at: 104 367 1990 2036 3244 104
Size: 263 1623 46 1208 1308
Fragments arranged by size:
1623 1308 1208 263 46
BsiHKAI G_wGCw'C
Cuts at: 125 191 302 974 1462 2240 2430 3222 125
Size: 66 111 672 488 778 190 792 1351
Fragments arranged by size:
1351 792 778 672 488 190 111 66
Bsp1286I G_dGCh'C
Cuts at: 125 191 211 290 302 317 347 422 653
Size: 66 20 79 12 15 30 75 231
Cuts at: 653 968 974 1462 2073 2166 2240 2430 2492
Size: 315 6 488 611 93 74 190 62
Cuts at: 2492 3222 125
Size: 730 1351
Fragments arranged by size:
1351 730 611 488 315 231 190 93
79 75 74 66 62 30 20 15
12 6
BspEI T'CCGG_A
Cuts at: 3668 3668
Size: 4448
BspHI T'CATG_A
Cuts at: 596 3624 596
Size: 3028 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 829 863 1498 1712 2014 2395 239
Size: 590 34 635 214 302 381 2292
Fragments arranged by size:
2292 635 590 381 302 214 34
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 271 2446 2627 2836 271
Size: 2175 181 209 1883
Fragments arranged by size:
2175 1883 209 181
BssSI C'ACGA_G
Cuts at: 301 2719 3077 301
Size: 2418 358 1672
Fragments arranged by size:
2418 1672 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstEII G'GTnAC_C
Cuts at: 775 813 775
Size: 38 4410
BstYI r'GATC_y
Cuts at: 1 306 356 569 1139 1473 1940 1963 2298
Size: 305 50 213 570 334 467 23 335
Cuts at: 2298 2544 3556 3642 3654 4000 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 570 467 449 346 335 334 305
246 213 86 50 23 12
BtgI C'CryG_G
Cuts at: 203 216 323 1026 203
Size: 13 107 703 3625
Fragments arranged by size:
3625 703 107 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 197 2398 2579 2742 197
Size: 2201 181 163 1903
Fragments arranged by size:
2201 1903 181 163
BtsI GCAGTG_nn'
Cuts at: 145 1691 145
Size: 1546 2902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1191 1226 1191
Size: 35 4413
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DraIII CAC_nnn'GTG
Cuts at: 792 792
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 364 1029 1223 1265 1439 2033 2207 2598 2625
Size: 665 194 42 174 594 174 391 27
Cuts at: 2625 3970 364
Size: 1345 842
Fragments arranged by size:
1345 842 665 594 391 194 174 174
42 27
EagI C'GGCC_G
Cuts at: 364 2033 364
Size: 1669 2779
EarI CTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1188 2656 2966 3112 4258 1188
Size: 1468 310 146 1146 1378
Fragments arranged by size:
1468 1378 1146 310 146
EcoICRI GAG'CTC
Cuts at: 972 1460 972
Size: 488 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 726 2273 2705 3452 3720 331
Size: 395 1547 432 747 268 1059
Fragments arranged by size:
1547 1059 747 432 395 268
EcoO109I rG'GnC_Cy
Cuts at: 343 344 1587 343
Size: 1 1243 3204
Fragments arranged by size:
3204 1243 1
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 161 958 1882 2130 3152 4131 161
Size: 797 924 248 1022 979 478
Fragments arranged by size:
1022 979 924 797 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1380 2290 2320 2322 2352 1348
Size: 32 910 30 2 30 3444
Fragments arranged by size:
3444 910 32 30 30 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 157 1878 2126 157
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
KpnI G_GTAC'C
Cuts at: 554 1468 554
Size: 914 3534
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3303 3119
Size: 184 4264
MscI TGG'CCA
Cuts at: 2209 2209
Size: 4448
MslI CAynn'nnrTG
Cuts at: 250 643 1072 1267 2437 2564 250
Size: 393 429 195 1170 127 2134
Fragments arranged by size:
2134 1170 429 393 195 127
MspA1I CmG'CkG
Cuts at: 237 510 1559 1873 2233 3246 3491 237
Size: 273 1049 314 360 1013 245 1194
Fragments arranged by size:
1194 1049 1013 360 314 273 245
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 158 1879 2127 158
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
NcoI C'CATG_G
Cuts at: 323 1026 323
Size: 703 3745
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NspI r_CATG'y
Cuts at: 1071 1501 2001 2908 1071
Size: 430 500 907 2611
Fragments arranged by size:
2611 907 500 430
PasI CC'CwG_GG
Cuts at: 522 522
Size: 4448
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PflMI CCAn_nnn'nTGG
Cuts at: 167 522 167
Size: 355 4093
PfoI T'CCnGG_A
Cuts at: 572 1229 572
Size: 657 3791
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1312 1344 1396 1428 3587 3619 1312
Size: 32 52 32 2159 32 2141
Fragments arranged by size:
2159 2141 52 32 32 32
PspOMI G'GGCC_C
Cuts at: 343 343
Size: 4448
PstI C_TGCA'G
Cuts at: 236 500 1495 2180 236
Size: 264 995 685 2504
Fragments arranged by size:
2504 995 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 237 510 1873 2233 237
Size: 273 1363 360 2452
Fragments arranged by size:
2452 1363 360 273
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 974 1462 974
Size: 488 3960
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 500 1495 500
Size: 995 3453
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SexAI A'CCwGG_T
Cuts at: 788 1358 788
Size: 570 3878
SfcI C'TryA_G
Cuts at: 33 232 496 1491 2176 3169 3360 33
Size: 199 264 995 685 993 191 1121
Fragments arranged by size:
1121 995 993 685 264 199 191
SfoI GGC'GCC
Cuts at: 159 1880 2128 159
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SgrAI Cr'CCGG_yG
Cuts at: 271 271
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1603 2518 3010 3272 1603
Size: 915 492 262 2779
Fragments arranged by size:
2779 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StuI AGG'CCT
Cuts at: 1285 1285
Size: 4448
StyI C'CwwG_G
Cuts at: 323 413 1026 1582 1936 323
Size: 90 613 556 354 2835
Fragments arranged by size:
2835 613 556 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 373 514 991 2031 2164 2623 2832 373
Size: 141 477 1040 133 459 209 1989
Fragments arranged by size:
1989 1040 477 459 209 141 133
TatI w'GTAC_w
Cuts at: 254 3963 254
Size: 3709 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 537 2245 537
Size: 1708 2740
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 523 1002 1080 1943 523
Size: 479 78 863 3028
Fragments arranged by size:
3028 863 479 78
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BbsI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI