Restriction map for
pF1KE1947
(Circular) (Six-base) MAPSORT of: pF1KE1947.seq Check: 7879 from: 1 to: 4616
pF1KE1947 4616 bp
With 182 enzymes: SgfI *
April 22, 2018 20:12 ..
AccI GT'mk_AC
Cuts at: 859 1654 859
Size: 795 3821
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1627 2441 2873 3620 3888 803
Size: 824 814 432 747 268 1531
Fragments arranged by size:
1531 824 814 747 432 268
AfeI AGC'GCT
Cuts at: 1362 1362
Size: 4616
AflIII A'CryG_T
Cuts at: 1968 3072 4049 1968
Size: 1104 977 2535
Fragments arranged by size:
2535 1104 977
AgeI A'CCGG_T
Cuts at: 3004 3004
Size: 4616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 4616
AlwNI CAG_nnn'CTG
Cuts at: 854 927 1921 3488 854
Size: 73 994 1567 1982
Fragments arranged by size:
1982 1567 994 73
ApaI G_GGCC'C
Cuts at: 1335 1335
Size: 4616
ApaLI G'TGCA_C
Cuts at: 1372 3386 1372
Size: 2014 2602
ApoI r'AATT_y
Cuts at: 1620 3969 4225 1620
Size: 2349 256 2011
Fragments arranged by size:
2349 2011 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 239 259 1636 239
Size: 20 1377 3219
Fragments arranged by size:
3219 1377 20
BamHI G'GATC_C
Cuts at: 1641 1641
Size: 4616
BanI G'GyrC_C
Cuts at: 169 214 532 979 1108 1632 2046 2294 2329
Size: 45 318 447 129 524 414 248 35
Cuts at: 2329 169
Size: 2456
Fragments arranged by size:
2456 524 447 414 318 248 129 45
35
BanII G_rGCy'C
Cuts at: 260 269 1335 1630 2660 260
Size: 9 1066 295 1030 2216
Fragments arranged by size:
2216 1066 1030 295 9
BbeI G_GCGC'C
Cuts at: 218 2050 2298 218
Size: 1832 248 2536
Fragments arranged by size:
2536 1832 248
BbsI GAAGACnn'nnnn_
Cuts at: 1436 1436
Size: 4616
BciVI GTATCCnnnnn_n'
Cuts at: 378 2514 3275 4616 378
Size: 2136 761 1341 378
Fragments arranged by size:
2136 1341 761 378
BclI T'GATC_A
Cuts at: 812 893 2136 812
Size: 81 1243 3292
Fragments arranged by size:
3292 1243 81
BglI GCCn_nnn'nGGC
Cuts at: 212 278 212
Size: 66 4550
BglII A'GATC_T
Cuts at: 2131 4168 2131
Size: 2037 2579
BlpI GC'TnA_GC
Cuts at: 1728 1728
Size: 4616
Bme1580I G_kGCm'C
Cuts at: 500 535 1282 1335 1376 2241 2334 3390 500
Size: 35 747 53 41 865 93 1056 1726
Fragments arranged by size:
1726 1056 865 747 93 53 41 35
BmgBI CAC'GTC
Cuts at: 1232 1232
Size: 4616
BmrI ACTGGGnnnn_n'
Cuts at: 642 1126 1828 1909 2244 4388 642
Size: 484 702 81 335 2144 870
Fragments arranged by size:
2144 870 702 484 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 709
Size: 4616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1792 2671 3163 3461 1792
Size: 879 492 298 2947
Fragments arranged by size:
2947 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 4320 238
Size: 4082 534
BsaAI yAC'GTr
Cuts at: 336 1969 336
Size: 1633 2983
BsaBI GATnn'nnATC
Cuts at: 6 2130 4167 6
Size: 2124 2037 455
Fragments arranged by size:
2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 215 2047 2295 2997 4493 215
Size: 1832 248 702 1496 338
Fragments arranged by size:
1832 1496 702 338 248
BsaWI w'CCGG_w
Cuts at: 479 1805 2326 3004 3278 3425 3836 479
Size: 1326 521 678 274 147 411 1259
Fragments arranged by size:
1326 1259 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 724 754 724
Size: 30 4586
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 1035 277
Size: 758 3858
BseYI C'CCAG_C
Cuts at: 173 528 2041 2799 3376 3872 173
Size: 355 1513 758 577 496 917
Fragments arranged by size:
1513 917 758 577 496 355
BsiEI CG_ry'CG
Cuts at: 104 273 2158 2204 3412 104
Size: 169 1885 46 1208 1308
Fragments arranged by size:
1885 1308 1208 169 46
BsiHKAI G_wGCw'C
Cuts at: 1376 1630 2408 2598 3390 1376
Size: 254 778 190 792 2602
Fragments arranged by size:
2602 792 778 254 190
Bsp1286I G_dGCh'C
Cuts at: 260 269 500 535 1282 1335 1376 1630 2241
Size: 9 231 35 747 53 41 254 611
Cuts at: 2241 2334 2408 2598 2660 3390 260
Size: 93 74 190 62 730 1486
Fragments arranged by size:
1486 747 730 611 254 231 190 93
74 62 53 41 35 9
BspEI T'CCGG_A
Cuts at: 479 3836 479
Size: 3357 1259
BspHI T'CATG_A
Cuts at: 1472 3792 1472
Size: 2320 2296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1214 1666 1880 2182 2563 1214
Size: 452 214 302 381 3267
Fragments arranged by size:
3267 452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2908 2962 4446 4611 50
Size: 2858 54 1484 165 55
Fragments arranged by size:
2858 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 584 2528 584
Size: 1944 2672
BsrFI r'CCGG_y
Cuts at: 2614 2795 3004 2614
Size: 181 209 4226
Fragments arranged by size:
4226 209 181
BsrGI T'GTAC_A
Cuts at: 839 839
Size: 4616
BssSI C'ACGA_G
Cuts at: 2887 3245 2887
Size: 358 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 412 1206 1993 412
Size: 794 787 3035
Fragments arranged by size:
3035 794 787
BstBI TT'CG_AA
Cuts at: 2977 2977
Size: 4616
BstEII G'GTnAC_C
Cuts at: 1161 1161
Size: 4616
BstYI r'GATC_y
Cuts at: 1 924 1641 2108 2131 2466 2712 3724 3810
Size: 923 717 467 23 335 246 1012 86
Cuts at: 3810 3822 4168 1
Size: 12 346 449
Fragments arranged by size:
1012 923 717 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 860 860
Size: 4616
Bsu36I CC'TnA_GG
Cuts at: 307 307
Size: 4616
BtgI C'CryG_G
Cuts at: 107 165 347 555 1049 1274 107
Size: 58 182 208 494 225 3449
Fragments arranged by size:
3449 494 225 208 182 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 421 2566 2747 2910 421
Size: 2145 181 163 2127
Fragments arranged by size:
2145 2127 181 163
BtsI GCAGTG_nn'
Cuts at: 413 1859 413
Size: 1446 3170
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4616
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2322 3180 1461
Size: 861 858 2897
Fragments arranged by size:
2897 861 858
EaeI y'GGCC_r
Cuts at: 270 1210 1226 2201 2375 2766 2793 4138 270
Size: 940 16 975 174 391 27 1345 748
Fragments arranged by size:
1345 975 940 748 391 174 27 16
EagI C'GGCC_G
Cuts at: 270 2201 270
Size: 1931 2685
EarI CTCTTCn'nnn_
Cuts at: 248 742 1516 2639 2849 248
Size: 494 774 1123 210 2015
Fragments arranged by size:
2015 1123 774 494 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2824 3134 3280 4426 2824
Size: 310 146 1146 3014
Fragments arranged by size:
3014 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1628 1628
Size: 4616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 803 1627 2441 2873 3620 3888 709
Size: 94 824 814 432 747 268 1437
Fragments arranged by size:
1437 824 814 747 432 268 94
EcoNI CCTnn'n_nnAGG
Cuts at: 481 1606 481
Size: 1125 3491
EcoO109I rG'GnC_Cy
Cuts at: 474 1405 1755 474
Size: 931 350 3335
Fragments arranged by size:
3335 931 350
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4616
FspI TGC'GCA
Cuts at: 199 2397 199
Size: 2198 2418
HaeII r_GCGC'y
Cuts at: 218 1364 1551 2050 2298 3320 4299 218
Size: 1146 187 499 248 1022 979 535
Fragments arranged by size:
1146 1022 979 535 499 248 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1283 1315 1582 1614 2458 2488 2490 2520 1283
Size: 32 267 32 844 30 2 30 3379
Fragments arranged by size:
3379 844 267 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1466 1655 1466
Size: 189 4427
KasI G'GCGC_C
Cuts at: 214 2046 2294 214
Size: 1832 248 2536
Fragments arranged by size:
2536 1832 248
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 653 780 3287 3471 653
Size: 127 2507 184 1798
Fragments arranged by size:
2507 1798 184 127
MscI TGG'CCA
Cuts at: 1228 2377 1228
Size: 1149 3467
MslI CAynn'nnrTG
Cuts at: 379 1285 2605 2732 379
Size: 906 1320 127 2263
Fragments arranged by size:
2263 1320 906 127
MspA1I CmG'CkG
Cuts at: 203 456 1089 1396 1559 1727 2041 2401 3414
Size: 253 633 307 163 168 314 360 1013
Cuts at: 3414 3659 203
Size: 245 1160
Fragments arranged by size:
1160 1013 633 360 314 307 253 245
168 163
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 215 2047 2295 215
Size: 1832 248 2536
Fragments arranged by size:
2536 1832 248
NcoI C'CATG_G
Cuts at: 107 555 107
Size: 448 4168
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NspI r_CATG'y
Cuts at: 1669 2169 3076 1669
Size: 500 907 3209
Fragments arranged by size:
3209 907 500
PasI CC'CwG_GG
Cuts at: 222 507 222
Size: 285 4331
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PflMI CCAn_nnn'nTGG
Cuts at: 1118 1118
Size: 4616
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4584
PpuMI rG'GwC_Cy
Cuts at: 474 474
Size: 4616
PspOMI G'GGCC_C
Cuts at: 1331 1331
Size: 4616
PstI C_TGCA'G
Cuts at: 749 1023 1663 2348 749
Size: 274 640 685 3017
Fragments arranged by size:
3017 685 640 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 456 1089 2041 2401 456
Size: 633 952 360 2671
Fragments arranged by size:
2671 952 633 360
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 1630 1630
Size: 4616
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 248 2639 2849 248
Size: 2391 210 2015
Fragments arranged by size:
2391 2015 210
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4616
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SexAI A'CCwGG_T
Cuts at: 539 539
Size: 4616
SfcI C'TryA_G
Cuts at: 33 745 1019 1659 2344 3337 3528 33
Size: 712 274 640 685 993 191 1121
Fragments arranged by size:
1121 993 712 685 640 274 191
SfoI GGC'GCC
Cuts at: 216 2048 2296 216
Size: 1832 248 2536
Fragments arranged by size:
2536 1832 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 241 1638 241
Size: 1397 3219
SmlI C'TyrA_G
Cuts at: 1771 2686 3178 3440 1771
Size: 915 492 262 2947
Fragments arranged by size:
2947 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
StuI AGG'CCT
Cuts at: 675 675
Size: 4616
StyI C'CwwG_G
Cuts at: 107 555 1117 1418 1750 2104 107
Size: 448 562 301 332 354 2619
Fragments arranged by size:
2619 562 448 354 332 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 262 400 520 879 1144 2199 2332 2791
Size: 75 138 120 359 265 1055 133 459
Cuts at: 2791 3000 187
Size: 209 1803
Fragments arranged by size:
1803 1055 459 359 265 209 138 133
120 75
TatI w'GTAC_w
Cuts at: 839 947 4131 839
Size: 108 3184 1324
Fragments arranged by size:
3184 1324 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1176 1208 4133 4165 1176
Size: 32 2925 32 1627
Fragments arranged by size:
2925 1627 32 32
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4616
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 4616
XmaI C'CCGG_G
Cuts at: 239 1636 239
Size: 1397 3219
XmnI GAAnn'nnTTC
Cuts at: 1126 1126
Size: 4616
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BssHII BstXI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI