Restriction map for
pF1KE1962
(Circular) (Six-base) MAPSORT of: pF1KE1962.seq Check: 7032 from: 1 to: 4694
pF1KE1962 4694 bp
With 182 enzymes: SgfI *
December 11, 2009 15:37 ..
AccI GT'mk_AC
Cuts at: 1732 1732
Size: 4694
Acc65I G'GTAC_C
Cuts at: 1710 1710
Size: 4694
AclI AA'CG_TT
Cuts at: 1424 4534 1424
Size: 3110 1584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1144 1528 2519 2951 3698 3966 655
Size: 489 384 991 432 747 268 1383
Fragments arranged by size:
1383 991 747 489 432 384 268
AflIII A'CryG_T
Cuts at: 2046 3150 4127 2046
Size: 1104 977 2613
Fragments arranged by size:
2613 1104 977
AgeI A'CCGG_T
Cuts at: 3082 3082
Size: 4694
AleI CACnn'nnGTG
Cuts at: 1642 1642
Size: 4694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 816 816
Size: 4694
AlwNI CAG_nnn'CTG
Cuts at: 1999 3566 1999
Size: 1567 3127
ApaI G_GGCC'C
Cuts at: 245 254 245
Size: 9 4685
ApaLI G'TGCA_C
Cuts at: 3464 3464
Size: 4694
ApoI r'AATT_y
Cuts at: 311 511 805 1520 1698 4047 4303 311
Size: 200 294 715 178 2349 256 702
Fragments arranged by size:
2349 715 702 294 256 200 178
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1250 1714 1250
Size: 464 4230
BamHI G'GATC_C
Cuts at: 123 576 639 1719 123
Size: 453 63 1080 3098
Fragments arranged by size:
3098 1080 453 63
BanI G'GyrC_C
Cuts at: 1710 2124 2372 2407 1710
Size: 414 248 35 3997
Fragments arranged by size:
3997 414 248 35
BanII G_rGCy'C
Cuts at: 245 254 1708 2738 245
Size: 9 1454 1030 2201
Fragments arranged by size:
2201 1454 1030 9
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 430 430
Size: 4694
BbvCI CC'TCA_GC
Cuts at: 1088 1088
Size: 4694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1269 1303 1269
Size: 34 4660
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3353 4694 2592
Size: 761 1341 2592
Fragments arranged by size:
2592 1341 761
BclI T'GATC_A
Cuts at: 2214 2214
Size: 4694
BglI GCCn_nnn'nGGC
Cuts at: 249 249
Size: 4694
BglII A'GATC_T
Cuts at: 2209 4246 2209
Size: 2037 2657
BlpI GC'TnA_GC
Cuts at: 1806 1806
Size: 4694
Bme1580I G_kGCm'C
Cuts at: 245 254 2319 2412 3468 245
Size: 9 2065 93 1056 1471
Fragments arranged by size:
2065 1471 1056 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 202 543 1036 1906 1987 2322 4466 202
Size: 341 493 870 81 335 2144 430
Fragments arranged by size:
2144 870 493 430 341 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 638 3061 638
Size: 2423 2271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 479 684 1384 1406 215
Size: 264 205 700 22 3503
Fragments arranged by size:
3503 700 264 205 22
Bpu10I CC'TnA_GC
Cuts at: 1088 1088
Size: 4694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2749 3241 3539 1870
Size: 879 492 298 3025
Fragments arranged by size:
3025 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4398 4398
Size: 4694
BsaAI yAC'GTr
Cuts at: 2047 2047
Size: 4694
BsaBI GATnn'nnATC
Cuts at: 6 731 753 2208 4245 6
Size: 725 22 1455 2037 455
Fragments arranged by size:
2037 1455 725 455 22
BsaHI Gr'CG_yC
Cuts at: 2125 2373 3075 4571 2125
Size: 248 702 1496 2248
Fragments arranged by size:
2248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 642 1064 1341 1651 1883 2404 3082 3356 3503
Size: 422 277 310 232 521 678 274 147
Cuts at: 3503 3914 642
Size: 411 1422
Fragments arranged by size:
1422 678 521 422 411 310 277 274
232 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 657 687 657
Size: 30 4664
BseYI C'CCAG_C
Cuts at: 2119 2877 3454 3950 2119
Size: 758 577 496 2863
Fragments arranged by size:
2863 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1666 1666
Size: 4694
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3490 104
Size: 2132 46 1208 1308
Fragments arranged by size:
2132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1251 1311 1341 1370 1708 2486 2676 3468 1251
Size: 60 30 29 338 778 190 792 2477
Fragments arranged by size:
2477 792 778 338 190 60 30 29
BsmI GAATG_Cn'
Cuts at: 1441 1632 1441
Size: 191 4503
Bsp1286I G_dGCh'C
Cuts at: 245 254 1251 1311 1341 1370 1708 2319 2412
Size: 9 997 60 30 29 338 611 93
Cuts at: 2412 2486 2676 2738 3468 245
Size: 74 190 62 730 1471
Fragments arranged by size:
1471 997 730 611 338 190 93 74
62 60 30 29 9
BspEI T'CCGG_A
Cuts at: 642 1341 1651 3914 642
Size: 699 310 2263 1422
Fragments arranged by size:
2263 1422 699 310
BspHI T'CATG_A
Cuts at: 956 3870 956
Size: 2914 1780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1958 2260 2641 1744
Size: 214 302 381 3797
Fragments arranged by size:
3797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 911 1493 2606 911
Size: 582 1113 2999
Fragments arranged by size:
2999 1113 582
BsrFI r'CCGG_y
Cuts at: 1498 2692 2873 3082 1498
Size: 1194 181 209 3110
Fragments arranged by size:
3110 1194 209 181
BssSI C'ACGA_G
Cuts at: 494 2965 3323 494
Size: 2471 358 1865
Fragments arranged by size:
2471 1865 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2071 2071
Size: 4694
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstEII G'GTnAC_C
Cuts at: 202 202
Size: 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 745 1384 745
Size: 639 4055
BstYI r'GATC_y
Cuts at: 1 123 537 576 639 876 897 1654 1719
Size: 122 414 39 63 237 21 757 65
Cuts at: 1719 2186 2209 2544 2790 3802 3888 3900 4246
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4246 1
Size: 449
Fragments arranged by size:
1012 757 467 449 414 346 335 246
237 122 86 65 63 39 23 21
12
BtgI C'CryG_G
Cuts at: 107 246 107
Size: 139 4555
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 417 718 1183 2644 2825 2988 417
Size: 301 465 1461 181 163 2123
Fragments arranged by size:
2123 1461 465 301 181 163
BtsI GCAGTG_nn'
Cuts at: 512 1406 1937 512
Size: 894 531 3269
Fragments arranged by size:
3269 894 531
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1284 1319 1284
Size: 35 4659
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3258 2400
Size: 858 3836
EaeI y'GGCC_r
Cuts at: 1256 1500 2279 2453 2844 2871 4216 1256
Size: 244 779 174 391 27 1345 1734
Fragments arranged by size:
1734 1345 779 391 244 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 1598 2717 2927 1598
Size: 1119 210 3365
Fragments arranged by size:
3365 1119 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3212 3358 4504 2902
Size: 310 146 1146 3092
Fragments arranged by size:
3092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1706 1706
Size: 4694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 479 655 684 1144 1384 1406 1528 2519
Size: 264 176 29 460 240 22 122 991
Cuts at: 2519 2951 3698 3966 215
Size: 432 747 268 943
Fragments arranged by size:
991 943 747 460 432 268 264 240
176 122 29 22
EcoO109I rG'GnC_Cy
Cuts at: 241 242 251 347 489 1207 1833 241
Size: 1 9 96 142 718 626 3102
Fragments arranged by size:
3102 718 626 142 96 9 1
EcoRI G'AATT_C
Cuts at: 311 1698 311
Size: 1387 3307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4694
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 2128 2376 3398 4377 2128
Size: 248 1022 979 2445
Fragments arranged by size:
2445 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 657 689 951 983 1464 1496 2536 2566 2568
Size: 32 262 32 481 32 1040 30 2
Cuts at: 2568 2598 657
Size: 30 2753
Fragments arranged by size:
2753 1040 481 262 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1733 1733
Size: 4694
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1714 1714
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 525 3365 3549 525
Size: 2840 184 1670
Fragments arranged by size:
2840 1670 184
MscI TGG'CCA
Cuts at: 1258 2455 1258
Size: 1197 3497
MslI CAynn'nnrTG
Cuts at: 743 1642 2683 2810 743
Size: 899 1041 127 2627
Fragments arranged by size:
2627 1041 899 127
MspA1I CmG'CkG
Cuts at: 162 475 1805 2119 2479 3492 3737 162
Size: 313 1330 314 360 1013 245 1119
Fragments arranged by size:
1330 1119 1013 360 314 313 245
NaeI GCC'GGC
Cuts at: 1500 2875 1500
Size: 1375 3319
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NcoI C'CATG_G
Cuts at: 107 107
Size: 4694
NgoMIV G'CCGG_C
Cuts at: 1498 2873 1498
Size: 1375 3319
NspI r_CATG'y
Cuts at: 334 1125 1290 1747 2247 3154 334
Size: 791 165 457 500 907 1874
Fragments arranged by size:
1874 907 791 500 457 165
PciI A'CATG_T
Cuts at: 3150 3150
Size: 4694
PflMI CCAn_nnn'nTGG
Cuts at: 1013 1203 1013
Size: 190 4504
PfoI T'CCnGG_A
Cuts at: 893 934 893
Size: 41 4653
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 4662
PpuMI rG'GwC_Cy
Cuts at: 489 1207 489
Size: 718 3976
PsiI TTA'TAA
Cuts at: 815 815
Size: 4694
PspOMI G'GGCC_C
Cuts at: 241 250 241
Size: 9 4685
PspXI vC'TCGA_Gb
Cuts at: 1250 1250
Size: 4694
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1225 1257 1225
Size: 32 4662
PstI C_TGCA'G
Cuts at: 1741 2426 1741
Size: 685 4009
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 475 2119 2479 475
Size: 1644 360 2690
Fragments arranged by size:
2690 1644 360
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1708 1708
Size: 4694
SalI G'TCGA_C
Cuts at: 1731 1731
Size: 4694
SanDI GG'GwC_CC
Cuts at: 489 1207 489
Size: 718 3976
SapI GCTCTTCn'nnn_
Cuts at: 1598 2717 2927 1598
Size: 1119 210 3365
Fragments arranged by size:
3365 1119 210
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SfcI C'TryA_G
Cuts at: 33 1737 2422 3415 3606 33
Size: 1704 685 993 191 1121
Fragments arranged by size:
1704 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 249 249
Size: 4694
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 1250 1849 2764 3256 3518 1250
Size: 599 915 492 262 2426
Fragments arranged by size:
2426 915 599 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 334 1290 1747 2247 334
Size: 956 457 500 2781
Fragments arranged by size:
2781 956 500 457
SspI AAT'ATT
Cuts at: 1381 1381
Size: 4694
StyI C'CwwG_G
Cuts at: 107 392 1007 1828 2182 107
Size: 285 615 821 354 2619
Fragments arranged by size:
2619 821 615 354 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 317 2277 2410 2869 3078 317
Size: 1960 133 459 209 1933
Fragments arranged by size:
1960 1933 459 209 133
TatI w'GTAC_w
Cuts at: 1604 4209 1604
Size: 2605 2089
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 4662
Tth111I GACn'n_nGTC
Cuts at: 649 2491 649
Size: 1842 2852
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 4694
XhoI C'TCGA_G
Cuts at: 1250 1250
Size: 4694
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
XmnI GAAnn'nnTTC
Cuts at: 980 980
Size: 4694
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BseRI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI SacII SexAI SgrAI
SnaBI SrfI StuI SwaI ZraI