Restriction map for
pF1KE1963
(Circular) (Six-base) MAPSORT of: pF1KE1963.seq Check: 3366 from: 1 to: 4685
pF1KE1963 4685 bp
With 182 enzymes: SgfI *
October 5, 2016 15:26 ..
AatII G_ACGT'C
Cuts at: 1013 1013
Size: 4685
AccI GT'mk_AC
Cuts at: 893 1478 1723 893
Size: 585 245 3855
Fragments arranged by size:
3855 585 245
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 954 1045 1482 2510 2942 3689 3957 925
Size: 29 91 437 1028 432 747 268 1653
Fragments arranged by size:
1653 1028 747 437 432 268 91 29
AfeI AGC'GCT
Cuts at: 967 967
Size: 4685
AflIII A'CryG_T
Cuts at: 1136 2037 3141 4118 1136
Size: 901 1104 977 1703
Fragments arranged by size:
1703 1104 977 901
AgeI A'CCGG_T
Cuts at: 3073 3073
Size: 4685
AlwNI CAG_nnn'CTG
Cuts at: 1990 3557 1990
Size: 1567 3118
ApaI G_GGCC'C
Cuts at: 212 565 671 212
Size: 353 106 4226
Fragments arranged by size:
4226 353 106
ApaLI G'TGCA_C
Cuts at: 1116 3455 1116
Size: 2339 2346
ApoI r'AATT_y
Cuts at: 1689 4038 4294 1689
Size: 2349 256 2080
Fragments arranged by size:
2349 2080 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 204 379 450 479 589 762 1076 1705 204
Size: 175 71 29 110 173 314 629 3184
Fragments arranged by size:
3184 629 314 175 173 110 71 29
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 199 1354 1701 2115 2363 2398 199
Size: 1155 347 414 248 35 2486
Fragments arranged by size:
2486 1155 414 347 248 35
BanII G_rGCy'C
Cuts at: 212 380 565 671 1385 1699 2729 212
Size: 168 185 106 714 314 1030 2168
Fragments arranged by size:
2168 1030 714 314 185 168 106
BbeI G_GCGC'C
Cuts at: 203 2119 2367 203
Size: 1916 248 2521
Fragments arranged by size:
2521 1916 248
BbsI GAAGACnn'nnnn_
Cuts at: 992 992
Size: 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 353 387 856 890 90
Size: 34 229 34 469 34 3885
Fragments arranged by size:
3885 469 229 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2583 3344 4685 2583
Size: 761 1341 2583
Fragments arranged by size:
2583 1341 761
BclI T'GATC_A
Cuts at: 2205 2205
Size: 4685
BglI GCCn_nnn'nGGC
Cuts at: 181 221 1096 181
Size: 40 875 3770
Fragments arranged by size:
3770 875 40
BglII A'GATC_T
Cuts at: 2200 4237 2200
Size: 2037 2648
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 212 565 671 1120 1193 1199 2310 2403 3459
Size: 353 106 449 73 6 1111 93 1056
Cuts at: 3459 212
Size: 1438
Fragments arranged by size:
1438 1111 1056 449 353 106 93 73
6
BmgBI CAC'GTC
Cuts at: 651 1590 651
Size: 939 3746
BmrI ACTGGGnnnn_n'
Cuts at: 1116 1897 1978 2313 4457 1116
Size: 781 81 335 2144 1344
Fragments arranged by size:
2144 1344 781 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1029 3052 1029
Size: 2023 2662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 592 964 982 1024 1645 412
Size: 180 372 18 42 621 3452
Fragments arranged by size:
3452 621 372 180 42 18
Bpu10I CC'TnA_GC
Cuts at: 1160 1519 1160
Size: 359 4326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3232 3530 1861
Size: 879 492 298 3016
Fragments arranged by size:
3016 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 523 4389 523
Size: 3866 819
BsaAI yAC'GTr
Cuts at: 1194 2038 1194
Size: 844 3841
BsaBI GATnn'nnATC
Cuts at: 6 2199 4236 6
Size: 2193 2037 455
Fragments arranged by size:
2193 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 173 200 320 1010 1249 1505 2116 2364
Size: 51 27 120 690 239 256 611 248
Cuts at: 2364 3066 4562 122
Size: 702 1496 245
Fragments arranged by size:
1496 702 690 611 256 248 245 239
120 51 27
BsaWI w'CCGG_w
Cuts at: 1874 2395 3073 3347 3494 3905 1874
Size: 521 678 274 147 411 2654
Fragments arranged by size:
2654 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 210 240 210
Size: 30 4655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 883 1417 424
Size: 459 534 3692
Fragments arranged by size:
3692 534 459
BseYI C'CCAG_C
Cuts at: 211 249 556 998 1185 2110 2868 3445 3941
Size: 38 307 442 187 925 758 577 496
Cuts at: 3941 211
Size: 955
Fragments arranged by size:
955 925 758 577 496 442 307 187
38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 1465 1372
Size: 93 4592
BsiEI CG_ry'CG
Cuts at: 104 359 1212 1442 2227 2273 3481 104
Size: 255 853 230 785 46 1208 1308
Fragments arranged by size:
1308 1208 853 785 255 230 46
BsiHKAI G_wGCw'C
Cuts at: 941 1120 1385 1699 2477 2667 3459 941
Size: 179 265 314 778 190 792 2167
Fragments arranged by size:
2167 792 778 314 265 190 179
BsmBI CGTCTCn'nnnn_
Cuts at: 493 1017 493
Size: 524 4161
Bsp1286I G_dGCh'C
Cuts at: 212 380 565 671 941 1120 1193 1199 1385
Size: 168 185 106 270 179 73 6 186
Cuts at: 1385 1699 2310 2403 2477 2667 2729 3459 212
Size: 314 611 93 74 190 62 730 1438
Fragments arranged by size:
1438 730 611 314 270 190 186 185
179 168 106 93 74 73 62 6
BspEI T'CCGG_A
Cuts at: 3905 3905
Size: 4685
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 446 1735 1949 2251 2632 446
Size: 1289 214 302 381 2499
Fragments arranged by size:
2499 1289 381 302 214
BsrBI CCG'CTC
Cuts at: 50 333 2977 3031 4515 4680 50
Size: 283 2644 54 1484 165 55
Fragments arranged by size:
2644 1484 283 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2597 2597
Size: 4685
BsrFI r'CCGG_y
Cuts at: 1060 2683 2864 3073 1060
Size: 1623 181 209 2672
Fragments arranged by size:
2672 1623 209 181
BssSI C'ACGA_G
Cuts at: 1177 2956 3314 1177
Size: 1779 358 2548
Fragments arranged by size:
2548 1779 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2062 2062
Size: 4685
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstEII G'GTnAC_C
Cuts at: 726 726
Size: 4685
BstYI r'GATC_y
Cuts at: 1 1710 2177 2200 2535 2781 3793 3879 3891
Size: 1709 467 23 335 246 1012 86 12
Cuts at: 3891 4237 1
Size: 346 449
Fragments arranged by size:
1709 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1430 1430
Size: 4685
BtgI C'CryG_G
Cuts at: 229 283 662 229
Size: 54 379 4252
Fragments arranged by size:
4252 379 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4341
Fragments arranged by size:
4341 181 163
BtsI GCAGTG_nn'
Cuts at: 1389 1928 1389
Size: 539 4146
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2391 3249 1470
Size: 921 858 2906
Fragments arranged by size:
2906 921 858
EaeI y'GGCC_r
Cuts at: 356 611 1058 1088 1205 1439 1580 2270 2444
Size: 255 447 30 117 234 141 690 174
Cuts at: 2444 2835 2862 4207 356
Size: 391 27 1345 834
Fragments arranged by size:
1345 834 690 447 391 255 234 174
141 117 30 27
EagI C'GGCC_G
Cuts at: 356 1439 2270 356
Size: 1083 831 2771
Fragments arranged by size:
2771 1083 831
EarI CTCTTCn'nnn_
Cuts at: 448 2708 2918 448
Size: 2260 210 2215
Fragments arranged by size:
2260 2215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1062 1120 2893 3203 3349 4495 1062
Size: 58 1773 310 146 1146 1252
Fragments arranged by size:
1773 1252 1146 310 146 58
EcoICRI GAG'CTC
Cuts at: 1383 1697 1383
Size: 314 4371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 592 925 954 964 982 1024 1045 1482
Size: 180 333 29 10 18 42 21 437
Cuts at: 1482 1645 2510 2942 3689 3957 412
Size: 163 865 432 747 268 1140
Fragments arranged by size:
1140 865 747 437 432 333 268 180
163 42 29 21 18 10
EcoNI CCTnn'n_nnAGG
Cuts at: 313 1399 313
Size: 1086 3599
EcoO109I rG'GnC_Cy
Cuts at: 208 278 384 561 562 606 667 675 909
Size: 70 106 177 1 44 61 8 234
Cuts at: 909 1259 1824 208
Size: 350 565 3069
Fragments arranged by size:
3069 565 350 234 177 106 70 61
44 8 1
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FseI GG_CCGG'CC
Cuts at: 1064 1064
Size: 4685
FspI TGC'GCA
Cuts at: 655 2466 655
Size: 1811 2874
HaeII r_GCGC'y
Cuts at: 203 475 969 1548 1620 2119 2367 3389 4368
Size: 272 494 579 72 499 248 1022 979
Cuts at: 4368 203
Size: 520
Fragments arranged by size:
1022 979 579 520 499 494 272 248
72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1270 1302 2527 2557 2559 2589 1270
Size: 32 1225 30 2 30 3366
Fragments arranged by size:
3366 1225 32 30 30 2
HincII GTy'rAC
Cuts at: 1724 1724
Size: 4685
KasI G'GCGC_C
Cuts at: 199 2115 2363 199
Size: 1916 248 2521
Fragments arranged by size:
2521 1916 248
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 1136 4118 1136
Size: 2982 1703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3356 3540 3356
Size: 184 4501
MscI TGG'CCA
Cuts at: 1090 1582 2446 1090
Size: 492 864 3329
Fragments arranged by size:
3329 864 492
MslI CAynn'nnrTG
Cuts at: 1588 2674 2801 1588
Size: 1086 127 3472
Fragments arranged by size:
3472 1086 127
MspA1I CmG'CkG
Cuts at: 253 285 355 361 664 974 1002 1047 1796
Size: 32 70 6 303 310 28 45 749
Cuts at: 1796 2110 2470 3483 3728 253
Size: 314 360 1013 245 1210
Fragments arranged by size:
1210 1013 749 360 314 310 303 245
70 45 32 28 6
NaeI GCC'GGC
Cuts at: 1062 2866 1062
Size: 1804 2881
NarI GG'CG_CC
Cuts at: 200 2116 2364 200
Size: 1916 248 2521
Fragments arranged by size:
2521 1916 248
NgoMIV G'CCGG_C
Cuts at: 1060 2864 1060
Size: 1804 2881
NotI GC'GGCC_GC
Cuts at: 356 356
Size: 4685
NspI r_CATG'y
Cuts at: 1738 2238 3145 1738
Size: 500 907 3278
Fragments arranged by size:
3278 907 500
PasI CC'CwG_GG
Cuts at: 1254 1254
Size: 4685
PciI A'CATG_T
Cuts at: 3141 3141
Size: 4685
PfoI T'CCnGG_A
Cuts at: 1634 1634
Size: 4685
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PmlI CAC'GTG
Cuts at: 1194 1194
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4653
PpuMI rG'GwC_Cy
Cuts at: 278 384 606 675 278
Size: 106 222 69 4288
Fragments arranged by size:
4288 222 106 69
PshAI GACnn'nnGTC
Cuts at: 713 713
Size: 4685
PspOMI G'GGCC_C
Cuts at: 208 561 667 208
Size: 353 106 4226
Fragments arranged by size:
4226 353 106
PspXI vC'TCGA_Gb
Cuts at: 589 589
Size: 4685
PstI C_TGCA'G
Cuts at: 246 366 1732 2417 246
Size: 120 1366 685 2514
Fragments arranged by size:
2514 1366 685 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 253 1002 1047 2110 2470 253
Size: 749 45 1063 360 2468
Fragments arranged by size:
2468 1063 749 360 45
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 1385 1699 1385
Size: 314 4371
SacII CC_GC'GG
Cuts at: 286 665 286
Size: 379 4306
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SanDI GG'GwC_CC
Cuts at: 384 384
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 448 2708 2918 448
Size: 2260 210 2215
Fragments arranged by size:
2260 2215 210
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 577 4202 577
Size: 3625 1060
SexAI A'CCwGG_T
Cuts at: 722 776 722
Size: 54 4631
SfcI C'TryA_G
Cuts at: 33 242 362 1728 2413 3406 3597 33
Size: 209 120 1366 685 993 191 1121
Fragments arranged by size:
1366 1121 993 685 209 191 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 1096 1096
Size: 4685
SfoI GGC'GCC
Cuts at: 201 2117 2365 201
Size: 1916 248 2521
Fragments arranged by size:
2521 1916 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 206 381 764 1078 1707 206
Size: 175 383 314 629 3184
Fragments arranged by size:
3184 629 383 314 175
SmlI C'TyrA_G
Cuts at: 589 1840 2755 3247 3509 589
Size: 1251 915 492 262 1765
Fragments arranged by size:
1765 1251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
StuI AGG'CCT
Cuts at: 949 1348 949
Size: 399 4286
StyI C'CwwG_G
Cuts at: 140 274 671 1508 1819 2173 140
Size: 134 397 837 311 354 2652
Fragments arranged by size:
2652 837 397 354 311 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1135 2268 2401 2860 3069 1135
Size: 1133 133 459 209 2751
Fragments arranged by size:
2751 1133 459 209 133
TatI w'GTAC_w
Cuts at: 575 824 857 1295 4200 575
Size: 249 33 438 2905 1060
Fragments arranged by size:
2905 1060 438 249 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 210 242 4202 4234 210
Size: 32 3960 32 661
Fragments arranged by size:
3960 661 32 32
Tth111I GACn'n_nGTC
Cuts at: 2482 2482
Size: 4685
XbaI T'CTAG_A
Cuts at: 525 1716 525
Size: 1191 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1590 2180 1590
Size: 590 4095
XhoI C'TCGA_G
Cuts at: 589 589
Size: 4685
XmaI C'CCGG_G
Cuts at: 204 379 762 1076 1705 204
Size: 175 383 314 629 3184
Fragments arranged by size:
3184 629 383 314 175
ZraI GAC'GTC
Cuts at: 1011 1011
Size: 4685
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NruI NsiI PacI
PflMI PsiI PsrI SgrAI SnaBI SrfI SspI SwaI
XmnI