(Circular) (Six-base) MAPSORT of: pF1KE1965.seq Check: 3105 from: 1 to: 4700 pF1KE1965 4700 bp With 182 enzymes: SgfI * July 3, 2020 20:21 .. AccI GT'mk_AC Cuts at: 1738 1738 Size: 4700 Acc65I G'GTAC_C Cuts at: 1716 1716 Size: 4700 AclI AA'CG_TT Cuts at: 4540 4540 Size: 4700 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1034 1652 2525 2957 3704 3972 1034 Size: 618 873 432 747 268 1762 Fragments arranged by size: 1762 873 747 618 432 268 AflIII A'CryG_T Cuts at: 2052 3156 4133 2052 Size: 1104 977 2619 Fragments arranged by size: 2619 1104 977 AgeI A'CCGG_T Cuts at: 3088 3088 Size: 4700 AhdI GACnn_n'nnGTC Cuts at: 339 339 Size: 4700 AleI CACnn'nnGTG Cuts at: 325 325 Size: 4700 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 755 787 755 Size: 32 4668 AlwNI CAG_nnn'CTG Cuts at: 780 2005 3572 780 Size: 1225 1567 1908 Fragments arranged by size: 1908 1567 1225 ApaLI G'TGCA_C Cuts at: 3470 3470 Size: 4700 ApoI r'AATT_y Cuts at: 505 1063 1704 4053 4309 505 Size: 558 641 2349 256 896 Fragments arranged by size: 2349 896 641 558 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4700 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4700 AvaI C'yCGr_G Cuts at: 985 1720 985 Size: 735 3965 BamHI G'GATC_C Cuts at: 1725 1725 Size: 4700 BanI G'GyrC_C Cuts at: 1316 1716 2130 2378 2413 1316 Size: 400 414 248 35 3603 Fragments arranged by size: 3603 414 400 248 35 BanII G_rGCy'C Cuts at: 1714 2744 1714 Size: 1030 3670 BbeI G_GCGC'C Cuts at: 2134 2382 2134 Size: 248 4452 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 469 503 90 Size: 34 345 34 4287 Fragments arranged by size: 4287 345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2598 3359 4700 2598 Size: 761 1341 2598 Fragments arranged by size: 2598 1341 761 BclI T'GATC_A Cuts at: 2220 2220 Size: 4700 BfrBI ATG'CAT Cuts at: 645 1279 645 Size: 634 4066 BglI GCCn_nnn'nGGC Cuts at: 1582 1582 Size: 4700 BglII A'GATC_T Cuts at: 1437 2215 4252 1437 Size: 778 2037 1885 Fragments arranged by size: 2037 1885 778 BlpI GC'TnA_GC Cuts at: 1812 1812 Size: 4700 Bme1580I G_kGCm'C Cuts at: 492 2325 2418 3474 492 Size: 1833 93 1056 1718 Fragments arranged by size: 1833 1718 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1172 1912 1993 2328 4472 1172 Size: 740 81 335 2144 1400 Fragments arranged by size: 2144 1400 740 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 4700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 711 711 Size: 4700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1876 2755 3247 3545 1876 Size: 879 492 298 3031 Fragments arranged by size: 3031 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4404 4404 Size: 4700 BsaAI yAC'GTr Cuts at: 2053 2053 Size: 4700 BsaBI GATnn'nnATC Cuts at: 6 2214 4251 6 Size: 2208 2037 455 Fragments arranged by size: 2208 2037 455 BsaHI Gr'CG_yC Cuts at: 2131 2379 3081 4577 2131 Size: 248 702 1496 2254 Fragments arranged by size: 2254 1496 702 248 BsaWI w'CCGG_w Cuts at: 1889 2410 3088 3362 3509 3920 1889 Size: 521 678 274 147 411 2669 Fragments arranged by size: 2669 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 228 258 228 Size: 30 4670 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 174 906 961 1639 174 Size: 732 55 678 3235 Fragments arranged by size: 3235 732 678 55 BseYI C'CCAG_C Cuts at: 900 2125 2883 3460 3956 900 Size: 1225 758 577 496 1644 Fragments arranged by size: 1644 1225 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 426 426 Size: 4700 BsiEI CG_ry'CG Cuts at: 104 2242 2288 3496 104 Size: 2138 46 1208 1308 Fragments arranged by size: 2138 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 382 1714 2492 2682 3474 382 Size: 1332 778 190 792 1608 Fragments arranged by size: 1608 1332 792 778 190 BsmI GAATG_Cn' Cuts at: 214 214 Size: 4700 BsmBI CGTCTCn'nnnn_ Cuts at: 444 444 Size: 4700 Bsp1286I G_dGCh'C Cuts at: 382 492 1714 2325 2418 2492 2682 2744 3474 Size: 110 1222 611 93 74 190 62 730 Cuts at: 3474 382 Size: 1608 Fragments arranged by size: 1608 1222 730 611 190 110 93 74 62 BspEI T'CCGG_A Cuts at: 3920 3920 Size: 4700 BspHI T'CATG_A Cuts at: 3876 3876 Size: 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 379 1750 1964 2266 2647 379 Size: 1371 214 302 381 2432 Fragments arranged by size: 2432 1371 381 302 214 BsrBI CCG'CTC Cuts at: 50 227 2992 3046 4530 4695 50 Size: 177 2765 54 1484 165 55 Fragments arranged by size: 2765 1484 177 165 55 54 BsrDI GCAATG_nn' Cuts at: 981 2612 981 Size: 1631 3069 BsrFI r'CCGG_y Cuts at: 2698 2879 3088 2698 Size: 181 209 4310 Fragments arranged by size: 4310 209 181 BsrGI T'GTAC_A Cuts at: 1403 1403 Size: 4700 BssSI C'ACGA_G Cuts at: 2971 3329 2971 Size: 358 4342 BstAPI GCAn_nnn'nTGC Cuts at: 2077 2077 Size: 4700 BstBI TT'CG_AA Cuts at: 3061 3061 Size: 4700 BstXI CCAn_nnnn'nTGG Cuts at: 114 382 1440 114 Size: 268 1058 3374 Fragments arranged by size: 3374 1058 268 BstYI r'GATC_y Cuts at: 1 679 1243 1437 1526 1725 2192 2215 2550 Size: 678 564 194 89 199 467 23 335 Cuts at: 2550 2796 3808 3894 3906 4252 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 678 564 467 449 346 335 246 199 194 89 86 23 12 BtgI C'CryG_G Cuts at: 220 1100 220 Size: 880 3820 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1567 2650 2831 2994 1567 Size: 1083 181 163 3273 Fragments arranged by size: 3273 1083 181 163 BtsI GCAGTG_nn' Cuts at: 667 1309 1571 1943 667 Size: 642 262 372 3424 Fragments arranged by size: 3424 642 372 262 DraI TTT'AAA Cuts at: 1699 1699 Size: 4700 DrdI GACnn_nn'nnGTC Cuts at: 634 2406 3264 634 Size: 1772 858 2070 Fragments arranged by size: 2070 1772 858 EaeI y'GGCC_r Cuts at: 159 246 576 2285 2459 2850 2877 4222 159 Size: 87 330 1709 174 391 27 1345 637 Fragments arranged by size: 1709 1345 637 391 330 174 87 27 EagI C'GGCC_G Cuts at: 2285 2285 Size: 4700 EarI CTCTTCn'nnn_ Cuts at: 751 1053 1331 2723 2933 751 Size: 302 278 1392 210 2518 Fragments arranged by size: 2518 1392 302 278 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2908 3218 3364 4510 2908 Size: 310 146 1146 3098 Fragments arranged by size: 3098 1146 310 146 EcoICRI GAG'CTC Cuts at: 1712 1712 Size: 4700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 711 1034 1652 2525 2957 3704 3972 711 Size: 323 618 873 432 747 268 1439 Fragments arranged by size: 1439 873 747 618 432 323 268 EcoO109I rG'GnC_Cy Cuts at: 368 569 991 1839 368 Size: 201 422 848 3229 Fragments arranged by size: 3229 848 422 201 EcoRI G'AATT_C Cuts at: 1704 1704 Size: 4700 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 441 2719 441 Size: 2278 2422 FspI TGC'GCA Cuts at: 2481 2481 Size: 4700 HaeII r_GCGC'y Cuts at: 2134 2382 3404 4383 2134 Size: 248 1022 979 2451 Fragments arranged by size: 2451 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 164 196 325 357 671 703 1465 1497 2542 Size: 32 129 32 314 32 762 32 1045 Cuts at: 2542 2572 2574 2604 164 Size: 30 2 30 2260 Fragments arranged by size: 2260 1045 762 314 129 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1287 1739 1287 Size: 452 4248 KasI G'GCGC_C Cuts at: 2130 2378 2130 Size: 248 4452 KpnI G_GTAC'C Cuts at: 1720 1720 Size: 4700 MluI A'CGCG_T Cuts at: 4133 4133 Size: 4700 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1504 3371 3555 1504 Size: 1867 184 2649 Fragments arranged by size: 2649 1867 184 MscI TGG'CCA Cuts at: 161 248 578 2461 161 Size: 87 330 1883 2400 Fragments arranged by size: 2400 1883 330 87 MslI CAynn'nnrTG Cuts at: 325 650 1213 2689 2816 325 Size: 325 563 1476 127 2209 Fragments arranged by size: 2209 1476 563 325 127 MspA1I CmG'CkG Cuts at: 157 1054 1811 2125 2485 3498 3743 157 Size: 897 757 314 360 1013 245 1114 Fragments arranged by size: 1114 1013 897 757 360 314 245 NaeI GCC'GGC Cuts at: 2881 2881 Size: 4700 NarI GG'CG_CC Cuts at: 2131 2379 2131 Size: 248 4452 NcoI C'CATG_G Cuts at: 1100 1100 Size: 4700 NgoMIV G'CCGG_C Cuts at: 2879 2879 Size: 4700 NsiI A_TGCA'T Cuts at: 647 1281 647 Size: 634 4066 NspI r_CATG'y Cuts at: 1194 1753 2253 3160 1194 Size: 559 500 907 2734 Fragments arranged by size: 2734 907 559 500 PciI A'CATG_T Cuts at: 3156 3156 Size: 4700 PflMI CCAn_nnn'nTGG Cuts at: 1162 1162 Size: 4700 PfoI T'CCnGG_A Cuts at: 1008 1008 Size: 4700 PmeI GTTT'AAAC Cuts at: 1699 1699 Size: 4700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 3839 Size: 32 4668 PpuMI rG'GwC_Cy Cuts at: 368 569 991 368 Size: 201 422 4077 Fragments arranged by size: 4077 422 201 PshAI GACnn'nnGTC Cuts at: 236 236 Size: 4700 PsiI TTA'TAA Cuts at: 362 362 Size: 4700 PstI C_TGCA'G Cuts at: 1747 2432 1747 Size: 685 4015 PvuI CG_AT'CG Cuts at: 104 104 Size: 4700 PvuII CAG'CTG Cuts at: 1054 2125 2485 1054 Size: 1071 360 3269 Fragments arranged by size: 3269 1071 360 RsrII CG'GwC_CG Cuts at: 2895 2895 Size: 4700 SacI G_AGCT'C Cuts at: 1714 1714 Size: 4700 SalI G'TCGA_C Cuts at: 1737 1737 Size: 4700 SapI GCTCTTCn'nnn_ Cuts at: 2723 2933 2723 Size: 210 4490 SbfI CC_TGCA'GG Cuts at: 1747 1747 Size: 4700 ScaI AGT'ACT Cuts at: 4217 4217 Size: 4700 SfcI C'TryA_G Cuts at: 33 933 1452 1743 2428 3421 3612 33 Size: 900 519 291 685 993 191 1121 Fragments arranged by size: 1121 993 900 685 519 291 191 SfoI GGC'GCC Cuts at: 2132 2380 2132 Size: 248 4452 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4700 SmaI CCC'GGG Cuts at: 1722 1722 Size: 4700 SmlI C'TyrA_G Cuts at: 1855 2770 3262 3524 1855 Size: 915 492 262 3031 Fragments arranged by size: 3031 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4700 SphI G_CATG'C Cuts at: 1194 1753 2253 1194 Size: 559 500 3641 Fragments arranged by size: 3641 559 500 SspI AAT'ATT Cuts at: 621 621 Size: 4700 StyI C'CwwG_G Cuts at: 733 1036 1100 1542 1834 2188 733 Size: 303 64 442 292 354 3245 Fragments arranged by size: 3245 442 354 303 292 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 615 1371 2283 2416 2875 3084 615 Size: 756 912 133 459 209 2231 Fragments arranged by size: 2231 912 756 459 209 133 TatI w'GTAC_w Cuts at: 125 857 950 1403 4215 125 Size: 732 93 453 2812 610 Fragments arranged by size: 2812 732 610 453 93 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 228 260 4217 4249 228 Size: 32 3957 32 679 Fragments arranged by size: 3957 679 32 32 Tth111I GACn'n_nGTC Cuts at: 553 2497 553 Size: 1944 2756 XbaI T'CTAG_A Cuts at: 1731 1731 Size: 4700 XcmI CCAnnnn_n'nnnnTGG Cuts at: 154 586 815 1090 2195 154 Size: 432 229 275 1105 2659 Fragments arranged by size: 2659 1105 432 275 229 XmaI C'CCGG_G Cuts at: 1720 1720 Size: 4700 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AlfI ApaI AscI AvrII BaeI BbsI BbvCI BmgBI BmtI Bpu10I BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PasI PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI