Restriction map for
pF1KE1978
(Circular) (Six-base) MAPSORT of: pF1KE1978.seq Check: 8852 from: 1 to: 4778
pF1KE1978 4778 bp
With 182 enzymes: SgfI *
June 30, 2019 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 331 838 1714 331
Size: 507 876 3395
Fragments arranged by size:
3395 876 507
AccI GT'mk_AC
Cuts at: 1816 1816
Size: 4778
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 4618 4618
Size: 4778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 597 2603 3035 3782 4050 470
Size: 127 2006 432 747 268 1198
Fragments arranged by size:
2006 1198 747 432 268 127
AflIII A'CryG_T
Cuts at: 2130 3234 4211 2130
Size: 1104 977 2697
Fragments arranged by size:
2697 1104 977
AgeI A'CCGG_T
Cuts at: 3166 3166
Size: 4778
AlwNI CAG_nnn'CTG
Cuts at: 369 1209 1449 2083 3650 369
Size: 840 240 634 1567 1497
Fragments arranged by size:
1567 1497 840 634 240
ApaI G_GGCC'C
Cuts at: 399 696 1073 1197 1538 399
Size: 297 377 124 341 3639
Fragments arranged by size:
3639 377 341 297 124
ApaLI G'TGCA_C
Cuts at: 3548 3548
Size: 4778
ApoI r'AATT_y
Cuts at: 1660 1782 4131 4387 1660
Size: 122 2349 256 2051
Fragments arranged by size:
2349 2051 256 122
AseI AT'TA_AT
Cuts at: 20 20
Size: 4778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 273 461 697 1072 1274 1798 273
Size: 188 236 375 202 524 3253
Fragments arranged by size:
3253 524 375 236 202 188
BamHI G'GATC_C
Cuts at: 1058 1803 1058
Size: 745 4033
BanI G'GyrC_C
Cuts at: 319 355 766 1591 1702 1794 2208 2456 2491
Size: 36 411 825 111 92 414 248 35
Cuts at: 2491 319
Size: 2606
Fragments arranged by size:
2606 825 414 411 248 111 92 36
35
BanII G_rGCy'C
Cuts at: 280 399 406 696 854 1073 1197 1224 1273
Size: 119 7 290 158 219 124 27 49
Cuts at: 1273 1538 1792 2822 280
Size: 265 254 1030 2236
Fragments arranged by size:
2236 1030 290 265 254 219 158 124
119 49 27 7
BbeI G_GCGC'C
Cuts at: 2212 2460 2212
Size: 248 4530
BbsI GAAGACnn'nnnn_
Cuts at: 595 595
Size: 4778
BbvCI CC'TCA_GC
Cuts at: 1450 1450
Size: 4778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1265 1299 1265
Size: 34 4744
BciVI GTATCCnnnnn_n'
Cuts at: 2676 3437 4778 2676
Size: 761 1341 2676
Fragments arranged by size:
2676 1341 761
BclI T'GATC_A
Cuts at: 1599 2298 1599
Size: 699 4079
BglI GCCn_nnn'nGGC
Cuts at: 275 275
Size: 4778
BglII A'GATC_T
Cuts at: 2293 4330 2293
Size: 2037 2741
BlpI GC'TnA_GC
Cuts at: 1305 1890 1305
Size: 585 4193
Bme1580I G_kGCm'C
Cuts at: 358 399 696 1073 1197 1252 1538 2403 2496
Size: 41 297 377 124 55 286 865 93
Cuts at: 2496 3552 358
Size: 1056 1584
Fragments arranged by size:
1584 1056 865 377 297 286 124 93
55 41
BmgBI CAC'GTC
Cuts at: 255 510 255
Size: 255 4523
BmrI ACTGGGnnnn_n'
Cuts at: 280 1990 2071 2406 4550 280
Size: 1710 81 335 2144 508
Fragments arranged by size:
2144 1710 508 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1098 3145 1098
Size: 2047 2731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1248 1378 1730 346
Size: 902 130 352 3394
Fragments arranged by size:
3394 902 352 130
Bpu10I CC'TnA_GC
Cuts at: 518 1450 518
Size: 932 3846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1954 2833 3325 3623 1954
Size: 879 492 298 3109
Fragments arranged by size:
3109 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1271 4482 1271
Size: 3211 1567
BsaAI yAC'GTr
Cuts at: 1149 2131 1149
Size: 982 3796
BsaBI GATnn'nnATC
Cuts at: 6 2292 4329 6
Size: 2286 2037 455
Fragments arranged by size:
2286 2037 455
BsaHI Gr'CG_yC
Cuts at: 2209 2457 3159 4655 2209
Size: 248 702 1496 2332
Fragments arranged by size:
2332 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1754 1967 2488 3166 3440 3587 3998 1754
Size: 213 521 678 274 147 411 2534
Fragments arranged by size:
2534 678 521 411 274 213 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 961 1203 1712 115
Size: 846 242 509 3181
Fragments arranged by size:
3181 846 509 242
BseYI C'CCAG_C
Cuts at: 731 792 1352 2203 2961 3538 4034 731
Size: 61 560 851 758 577 496 1475
Fragments arranged by size:
1475 851 758 577 560 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 658 1129 309
Size: 349 471 3958
Fragments arranged by size:
3958 471 349
BsiEI CG_ry'CG
Cuts at: 104 1246 2320 2366 3574 104
Size: 1142 1074 46 1208 1308
Fragments arranged by size:
1308 1208 1142 1074 46
BsiHKAI G_wGCw'C
Cuts at: 1462 1520 1792 2570 2760 3552 1462
Size: 58 272 778 190 792 2688
Fragments arranged by size:
2688 792 778 272 190 58
Bsp1286I G_dGCh'C
Cuts at: 280 358 399 406 696 854 1073 1197 1224
Size: 78 41 7 290 158 219 124 27
Cuts at: 1224 1252 1273 1462 1520 1538 1792 2403 2496
Size: 28 21 189 58 18 254 611 93
Cuts at: 2496 2570 2760 2822 3552 280
Size: 74 190 62 730 1506
Fragments arranged by size:
1506 730 611 290 254 219 190 189
158 124 93 78 74 62 58 41
28 27 21 18 7
BspEI T'CCGG_A
Cuts at: 3998 3998
Size: 4778
BspHI T'CATG_A
Cuts at: 3954 3954
Size: 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 331 713 838 1055 1539 1714 1828 2042 2344
Size: 382 125 217 484 175 114 214 302
Cuts at: 2344 2725 331
Size: 381 2384
Fragments arranged by size:
2384 484 382 381 302 217 214 175
125 114
BsrBI CCG'CTC
Cuts at: 50 272 3070 3124 4608 4773 50
Size: 222 2798 54 1484 165 55
Fragments arranged by size:
2798 1484 222 165 55 54
BsrDI GCAATG_nn'
Cuts at: 537 2690 537
Size: 2153 2625
BsrFI r'CCGG_y
Cuts at: 550 1241 2776 2957 3166 550
Size: 691 1535 181 209 2162
Fragments arranged by size:
2162 1535 691 209 181
BssSI C'ACGA_G
Cuts at: 3049 3407 3049
Size: 358 4420
BstAPI GCAn_nnn'nTGC
Cuts at: 1152 2155 1152
Size: 1003 3775
BstBI TT'CG_AA
Cuts at: 3139 3139
Size: 4778
BstEII G'GTnAC_C
Cuts at: 1311 1362 1311
Size: 51 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 406 406
Size: 4778
BstYI r'GATC_y
Cuts at: 1 1058 1803 2270 2293 2628 2874 3886 3972
Size: 1057 745 467 23 335 246 1012 86
Cuts at: 3972 3984 4330 1
Size: 12 346 449
Fragments arranged by size:
1057 1012 745 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 745 1540 745
Size: 795 3983
BtgI C'CryG_G
Cuts at: 107 912 1171 1299 1409 107
Size: 805 259 128 110 3476
Fragments arranged by size:
3476 805 259 128 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2728 2909 3072 2728
Size: 181 163 4434
Fragments arranged by size:
4434 181 163
BtsI GCAGTG_nn'
Cuts at: 815 1403 2021 815
Size: 588 618 3572
Fragments arranged by size:
3572 618 588
DraI TTT'AAA
Cuts at: 1777 1777
Size: 4778
DraIII CAC_nnn'GTG
Cuts at: 1466 1466
Size: 4778
DrdI GACnn_nn'nnGTC
Cuts at: 496 2484 3342 496
Size: 1988 858 1932
Fragments arranged by size:
1988 1932 858
EaeI y'GGCC_r
Cuts at: 236 548 798 1023 1243 1440 1523 1650 2363
Size: 312 250 225 220 197 83 127 713
Cuts at: 2363 2537 2928 2955 4300 236
Size: 174 391 27 1345 714
Fragments arranged by size:
1345 714 713 391 312 250 225 220
197 174 127 83 27
EagI C'GGCC_G
Cuts at: 1243 2363 1243
Size: 1120 3658
EarI CTCTTCn'nnn_
Cuts at: 1261 1435 2801 3011 1261
Size: 174 1366 210 3028
Fragments arranged by size:
3028 1366 210 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 256 2986 3296 3442 4588 256
Size: 2730 310 146 1146 446
Fragments arranged by size:
2730 1146 446 310 146
EcoICRI GAG'CTC
Cuts at: 1790 1790
Size: 4778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 470 597 1248 1378 1730 2603 3035 3782
Size: 124 127 651 130 352 873 432 747
Cuts at: 3782 4050 346
Size: 268 1074
Fragments arranged by size:
1074 873 747 651 432 352 268 130
127 124
EcoNI CCTnn'n_nnAGG
Cuts at: 349 744 349
Size: 395 4383
EcoO109I rG'GnC_Cy
Cuts at: 692 693 702 1167 1193 1194 1344 1383 1534
Size: 1 9 465 26 1 150 39 151
Cuts at: 1534 1535 1917 692
Size: 1 382 3553
Fragments arranged by size:
3553 465 382 151 150 39 26 9
1 1 1
EcoRI G'AATT_C
Cuts at: 1782 1782
Size: 4778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 2797 1449
Size: 1348 3430
FspI TGC'GCA
Cuts at: 826 2559 826
Size: 1733 3045
HaeII r_GCGC'y
Cuts at: 369 382 420 1005 2212 2460 3482 4461 369
Size: 13 38 585 1207 248 1022 979 686
Fragments arranged by size:
1207 1022 979 686 585 248 38 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1581 2620 2650 2652 2682 1549
Size: 32 1039 30 2 30 3645
Fragments arranged by size:
3645 1039 32 30 30 2
HincII GTy'rAC
Cuts at: 900 1817 900
Size: 917 3861
KasI G'GCGC_C
Cuts at: 2208 2456 2208
Size: 248 4530
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MluI A'CGCG_T
Cuts at: 4211 4211
Size: 4778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 782 3449 3633 782
Size: 2667 184 1927
Fragments arranged by size:
2667 1927 184
MscI TGG'CCA
Cuts at: 1442 1525 1652 2539 1442
Size: 83 127 887 3681
Fragments arranged by size:
3681 887 127 83
MslI CAynn'nnrTG
Cuts at: 1287 2767 2894 1287
Size: 1480 127 3171
Fragments arranged by size:
3171 1480 127
MspA1I CmG'CkG
Cuts at: 672 1173 1309 1356 1889 2203 2563 3576 3821
Size: 501 136 47 533 314 360 1013 245
Cuts at: 3821 672
Size: 1629
Fragments arranged by size:
1629 1013 533 501 360 314 245 136
47
NaeI GCC'GGC
Cuts at: 552 1243 2959 552
Size: 691 1716 2371
Fragments arranged by size:
2371 1716 691
NarI GG'CG_CC
Cuts at: 2209 2457 2209
Size: 248 4530
NcoI C'CATG_G
Cuts at: 107 1299 107
Size: 1192 3586
NgoMIV G'CCGG_C
Cuts at: 550 1241 2957 550
Size: 691 1716 2371
Fragments arranged by size:
2371 1716 691
NspI r_CATG'y
Cuts at: 541 1831 2331 3238 541
Size: 1290 500 907 2081
Fragments arranged by size:
2081 1290 907 500
PasI CC'CwG_GG
Cuts at: 399 399
Size: 4778
PciI A'CATG_T
Cuts at: 3234 3234
Size: 4778
PfoI T'CCnGG_A
Cuts at: 1746 1746
Size: 4778
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PmlI CAC'GTG
Cuts at: 1149 1149
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4746
PpuMI rG'GwC_Cy
Cuts at: 702 1167 702
Size: 465 4313
PspOMI G'GGCC_C
Cuts at: 395 692 1069 1193 1534 395
Size: 297 377 124 341 3639
Fragments arranged by size:
3639 377 341 297 124
PstI C_TGCA'G
Cuts at: 1240 1825 2510 1240
Size: 585 685 3508
Fragments arranged by size:
3508 685 585
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 1356 2203 2563 1356
Size: 847 360 3571
Fragments arranged by size:
3571 847 360
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 1792 1792
Size: 4778
SacII CC_GC'GG
Cuts at: 1174 1174
Size: 4778
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 1261 1435 2801 3011 1261
Size: 174 1366 210 3028
Fragments arranged by size:
3028 1366 210 174
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 1081 4295 1081
Size: 3214 1564
SfcI C'TryA_G
Cuts at: 33 346 642 1236 1821 2506 3499 3690 33
Size: 313 296 594 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 594 585 313 296 191
SfoI GGC'GCC
Cuts at: 2210 2458 2210
Size: 248 4530
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 463 1800 463
Size: 1337 3441
SmlI C'TyrA_G
Cuts at: 1933 2848 3340 3602 1933
Size: 915 492 262 3109
Fragments arranged by size:
3109 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4778
SphI G_CATG'C
Cuts at: 1831 2331 1831
Size: 500 4278
StuI AGG'CCT
Cuts at: 940 940
Size: 4778
StyI C'CwwG_G
Cuts at: 107 935 1299 1587 1912 2266 107
Size: 828 364 288 325 354 2619
Fragments arranged by size:
2619 828 364 354 325 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 745 784 1333 2361 2494 2953 3162 745
Size: 39 549 1028 133 459 209 2361
Fragments arranged by size:
2361 1028 549 459 209 133 39
TatI w'GTAC_w
Cuts at: 605 1079 4293 605
Size: 474 3214 1090
Fragments arranged by size:
3214 1090 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 4295 4327 287
Size: 32 3976 32 738
Fragments arranged by size:
3976 738 32 32
Tth111I GACn'n_nGTC
Cuts at: 1717 2575 1717
Size: 858 3920
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 2273 453
Size: 1820 2958
XmaI C'CCGG_G
Cuts at: 461 1798 461
Size: 1337 3441
XmnI GAAnn'nnTTC
Cuts at: 1429 1429
Size: 4778
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI