Restriction map for
pF1KE1986
(Circular) (Six-base) MAPSORT of: pF1KE1986.seq Check: 6812 from: 1 to: 4763
pF1KE1986 4763 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 763 763
Size: 4763
AccI GT'mk_AC
Cuts at: 1801 1801
Size: 4763
Acc65I G'GTAC_C
Cuts at: 1195 1779 1195
Size: 584 4179
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 3020 3767 4035 2588
Size: 432 747 268 3316
Fragments arranged by size:
3316 747 432 268
AfeI AGC'GCT
Cuts at: 1399 1399
Size: 4763
AflII C'TTAA_G
Cuts at: 1089 1089
Size: 4763
AflIII A'CryG_T
Cuts at: 968 2115 3219 4196 968
Size: 1147 1104 977 1535
Fragments arranged by size:
1535 1147 1104 977
AgeI A'CCGG_T
Cuts at: 3151 3151
Size: 4763
AlwNI CAG_nnn'CTG
Cuts at: 199 690 2068 3635 199
Size: 491 1378 1567 1327
Fragments arranged by size:
1567 1378 1327 491
ApaLI G'TGCA_C
Cuts at: 3533 3533
Size: 4763
ApoI r'AATT_y
Cuts at: 676 745 1767 4116 4372 676
Size: 69 1022 2349 256 1067
Fragments arranged by size:
2349 1067 1022 256 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 1783 1783
Size: 4763
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 228 331 1195 1779 2193 2441 2476 228
Size: 103 864 584 414 248 35 2515
Fragments arranged by size:
2515 864 584 414 248 103 35
BanII G_rGCy'C
Cuts at: 1088 1648 1777 2807 1088
Size: 560 129 1030 3044
Fragments arranged by size:
3044 1030 560 129
BbeI G_GCGC'C
Cuts at: 2197 2445 2197
Size: 248 4515
BbsI GAAGACnn'nnnn_
Cuts at: 128 1131 128
Size: 1003 3760
BbvCI CC'TCA_GC
Cuts at: 189 189
Size: 4763
BciVI GTATCCnnnnn_n'
Cuts at: 547 694 2661 3422 4763 547
Size: 147 1967 761 1341 547
Fragments arranged by size:
1967 1341 761 547 147
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4763
BfrBI ATG'CAT
Cuts at: 834 834
Size: 4763
BglII A'GATC_T
Cuts at: 2278 4315 2278
Size: 2037 2726
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4763
Bme1580I G_kGCm'C
Cuts at: 2388 2481 3537 2388
Size: 93 1056 3614
Fragments arranged by size:
3614 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 431 1658 1975 2056 2391 4535 431
Size: 1227 317 81 335 2144 659
Fragments arranged by size:
2144 1227 659 335 317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 1479 620
Size: 859 3904
Bpu10I CC'TnA_GC
Cuts at: 189 189
Size: 4763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3310 3608 1939
Size: 879 492 298 3094
Fragments arranged by size:
3094 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 204 1536 4467 204
Size: 1332 2931 500
Fragments arranged by size:
2931 1332 500
BsaAI yAC'GTr
Cuts at: 414 2116 414
Size: 1702 3061
BsaBI GATnn'nnATC
Cuts at: 6 1054 2277 4314 6
Size: 1048 1223 2037 455
Fragments arranged by size:
2037 1223 1048 455
BsaHI Gr'CG_yC
Cuts at: 112 2194 2442 3144 4640 112
Size: 2082 248 702 1496 235
Fragments arranged by size:
2082 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1952 2473 3151 3425 3572 3983 1952
Size: 521 678 274 147 411 2732
Fragments arranged by size:
2732 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 357 387 1340 1370 357
Size: 30 953 30 3750
Fragments arranged by size:
3750 953 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 111 111
Size: 4763
BseYI C'CCAG_C
Cuts at: 2188 2946 3523 4019 2188
Size: 758 577 496 2932
Fragments arranged by size:
2932 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 4763
BsiEI CG_ry'CG
Cuts at: 104 143 2305 2351 3559 104
Size: 39 2162 46 1208 1308
Fragments arranged by size:
2162 1308 1208 46 39
BsiHKAI G_wGCw'C
Cuts at: 1322 1777 2555 2745 3537 1322
Size: 455 778 190 792 2548
Fragments arranged by size:
2548 792 778 455 190
Bsp1286I G_dGCh'C
Cuts at: 1088 1322 1648 1777 2388 2481 2555 2745 2807
Size: 234 326 129 611 93 74 190 62
Cuts at: 2807 3537 1088
Size: 730 2314
Fragments arranged by size:
2314 730 611 326 234 190 129 93
74 62
BspEI T'CCGG_A
Cuts at: 3983 3983
Size: 4763
BspHI T'CATG_A
Cuts at: 3939 3939
Size: 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 1813 2027 2329 2710 763
Size: 1050 214 302 381 2816
Fragments arranged by size:
2816 1050 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3055 3109 4593 4758 50
Size: 3005 54 1484 165 55
Fragments arranged by size:
3005 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2675 2675
Size: 4763
BsrFI r'CCGG_y
Cuts at: 1128 2761 2942 3151 1128
Size: 1633 181 209 2740
Fragments arranged by size:
2740 1633 209 181
BsrGI T'GTAC_A
Cuts at: 971 971
Size: 4763
BssSI C'ACGA_G
Cuts at: 3034 3392 3034
Size: 358 4405
BstAPI GCAn_nnn'nTGC
Cuts at: 505 690 1508 2140 505
Size: 185 818 632 3128
Fragments arranged by size:
3128 818 632 185
BstBI TT'CG_AA
Cuts at: 3124 3124
Size: 4763
BstXI CCAn_nnnn'nTGG
Cuts at: 1161 1222 1161
Size: 61 4702
BstYI r'GATC_y
Cuts at: 1 1788 2255 2278 2613 2859 3871 3957 3969
Size: 1787 467 23 335 246 1012 86 12
Cuts at: 3969 4315 1
Size: 346 449
Fragments arranged by size:
1787 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 285 285
Size: 4763
BtgI C'CryG_G
Cuts at: 107 164 215 1160 1199 107
Size: 57 51 945 39 3671
Fragments arranged by size:
3671 945 57 51 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1351 2713 2894 3057 1351
Size: 1362 181 163 3057
Fragments arranged by size:
3057 1362 181 163
BtsI GCAGTG_nn'
Cuts at: 576 1101 2006 576
Size: 525 905 3333
Fragments arranged by size:
3333 905 525
DraI TTT'AAA
Cuts at: 915 1762 915
Size: 847 3916
DraIII CAC_nnn'GTG
Cuts at: 360 360
Size: 4763
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3327 2469
Size: 858 3905
EaeI y'GGCC_r
Cuts at: 2348 2522 2913 2940 4285 2348
Size: 174 391 27 1345 2826
Fragments arranged by size:
2826 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4763
EarI CTCTTCn'nnn_
Cuts at: 908 2786 2996 908
Size: 1878 210 2675
Fragments arranged by size:
2675 1878 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 688 2971 3281 3427 4573 688
Size: 2283 310 146 1146 878
Fragments arranged by size:
2283 1146 878 310 146
EcoICRI GAG'CTC
Cuts at: 1775 1775
Size: 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 1479 2588 3020 3767 4035 620
Size: 859 1109 432 747 268 1348
Fragments arranged by size:
1348 1109 859 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1902 1902
Size: 4763
EcoRI G'AATT_C
Cuts at: 1767 1767
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 2782 1449
Size: 1333 3430
FspI TGC'GCA
Cuts at: 2544 2544
Size: 4763
HaeII r_GCGC'y
Cuts at: 1401 2197 2445 3467 4446 1401
Size: 796 248 1022 979 1718
Fragments arranged by size:
1718 1022 979 796 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 447 479 792 824 1340 1342 1372 1374 2605
Size: 32 313 32 516 2 30 2 1231
Cuts at: 2605 2635 2637 2667 447
Size: 30 2 30 2543
Fragments arranged by size:
2543 1231 516 313 32 32 30 30
30 2 2 2
HincII GTy'rAC
Cuts at: 1802 1802
Size: 4763
HindIII A'AGCT_T
Cuts at: 1726 1726
Size: 4763
KasI G'GCGC_C
Cuts at: 2193 2441 2193
Size: 248 4515
KpnI G_GTAC'C
Cuts at: 1199 1783 1199
Size: 584 4179
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 684 1295 1662 3434 3618 684
Size: 611 367 1772 184 1829
Fragments arranged by size:
1829 1772 611 367 184
MscI TGG'CCA
Cuts at: 2524 2524
Size: 4763
MslI CAynn'nnrTG
Cuts at: 272 473 1159 1220 2752 2879 272
Size: 201 686 61 1532 127 2156
Fragments arranged by size:
2156 1532 686 201 127 61
MspA1I CmG'CkG
Cuts at: 166 579 1874 2188 2548 3561 3806 166
Size: 413 1295 314 360 1013 245 1123
Fragments arranged by size:
1295 1123 1013 413 360 314 245
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4763
NarI GG'CG_CC
Cuts at: 2194 2442 2194
Size: 248 4515
NcoI C'CATG_G
Cuts at: 107 215 1160 1199 107
Size: 108 945 39 3671
Fragments arranged by size:
3671 945 108 39
NdeI CA'TA_TG
Cuts at: 1217 1217
Size: 4763
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4763
NsiI A_TGCA'T
Cuts at: 836 836
Size: 4763
NspI r_CATG'y
Cuts at: 249 972 1816 2316 3223 249
Size: 723 844 500 907 1789
Fragments arranged by size:
1789 907 844 723 500
PciI A'CATG_T
Cuts at: 968 3219 968
Size: 2251 2512
PflMI CCAn_nnn'nTGG
Cuts at: 360 360
Size: 4763
PfoI T'CCnGG_A
Cuts at: 521 696 521
Size: 175 4588
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1732 1764 3902 3934 1732
Size: 32 2138 32 2561
Fragments arranged by size:
2561 2138 32 32
PstI C_TGCA'G
Cuts at: 571 686 998 1019 1810 2495 571
Size: 115 312 21 791 685 2839
Fragments arranged by size:
2839 791 685 312 115 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 2188 2548 2188
Size: 360 4403
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1777 1777
Size: 4763
SacII CC_GC'GG
Cuts at: 167 167
Size: 4763
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 2786 2996 2786
Size: 210 4553
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 1108 4280 1108
Size: 3172 1591
SfcI C'TryA_G
Cuts at: 33 567 591 682 994 1015 1288 1806 2491
Size: 534 24 91 312 21 273 518 685
Cuts at: 2491 3484 3675 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 534 518 312 273 191
91 24 21
SfoI GGC'GCC
Cuts at: 2195 2443 2195
Size: 248 4515
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 1785 1785
Size: 4763
SmlI C'TyrA_G
Cuts at: 1089 1918 2833 3325 3587 1089
Size: 829 915 492 262 2265
Fragments arranged by size:
2265 915 829 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 249 1816 2316 249
Size: 1567 500 2696
Fragments arranged by size:
2696 1567 500
StyI C'CwwG_G
Cuts at: 107 215 1160 1199 1590 1718 1897 2251 107
Size: 108 945 39 391 128 179 354 2619
Fragments arranged by size:
2619 945 391 354 179 128 108 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 1210 1451 1518 1768 2346 2479 2938 3147
Size: 1081 241 67 250 578 133 459 209
Cuts at: 3147 129
Size: 1745
Fragments arranged by size:
1745 1081 578 459 250 241 209 133
67
TatI w'GTAC_w
Cuts at: 758 971 1106 1711 4278 758
Size: 213 135 605 2567 1243
Fragments arranged by size:
2567 1243 605 213 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4280 4312 4280
Size: 32 4731
Tth111I GACn'n_nGTC
Cuts at: 1354 2560 1354
Size: 1206 3557
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2258 2258
Size: 4763
XmaI C'CCGG_G
Cuts at: 1783 1783
Size: 4763
XmnI GAAnn'nnTTC
Cuts at: 1256 1256
Size: 4763
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI