FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KE2008, 583 aa 1>>>pF1KE2008 583 - 583 aa - 583 aa Library: human.CCDS.faa 18921897 residues in 33420 sequences Statistics: Expectation_n fit: rho(ln(x))= 6.8786+/-0.00116; mu= 11.2293+/- 0.069 mean_var=124.8269+/-26.012, 0's: 0 Z-trim(105.0): 104 B-trim: 94 in 1/51 Lambda= 0.114794 statistics sampled from 8180 (8286) to 8180 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.609), E-opt: 0.2 (0.248), width: 16 Scan time: 1.470 The best scores are: opt bits E(33420) CCDS33810.1 ALCAM gene_id:214|Hs109|chr3 ( 583) 3823 645.2 6.6e-185 CCDS58841.1 ALCAM gene_id:214|Hs109|chr3 ( 570) 3320 561.9 7.7e-160 CCDS31690.1 MCAM gene_id:4162|Hs109|chr11 ( 646) 453 87.2 7.3e-17 >>CCDS33810.1 ALCAM gene_id:214|Hs109|chr3 (583 aa) initn: 3823 init1: 3823 opt: 3823 Z-score: 3433.4 bits: 645.2 E(33420): 6.6e-185 Smith-Waterman score: 3823; 100.0% identity (100.0% similar) in 583 aa overlap (1-583:1-583) 10 20 30 40 50 60 pF1KE2 MESKGASSCRLLFCLLISATVFRPGLGWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 MESKGASSCRLLFCLLISATVFRPGLGWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEK 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE2 PDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 PDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDN 70 80 90 100 110 120 130 140 150 160 170 180 pF1KE2 VFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 VFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPL 130 140 150 160 170 180 190 200 210 220 230 240 pF1KE2 EGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 EGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVF 190 200 210 220 230 240 250 260 270 280 290 300 pF1KE2 DIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 DIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTL 250 260 270 280 290 300 310 320 330 340 350 360 pF1KE2 TDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 TDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASR 310 320 330 340 350 360 370 380 390 400 410 420 pF1KE2 NATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 NATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKM 370 380 390 400 410 420 430 440 450 460 470 480 pF1KE2 TKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEEN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 TKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEEN 430 440 450 460 470 480 490 500 510 520 530 540 pF1KE2 VTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAKLIVGIVVGLLLAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 VTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAKLIVGIVVGLLLAA 490 500 510 520 530 540 550 560 570 580 pF1KE2 LVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKLEENNHKTEA ::::::::::::::::::::::::::::::::::::::::::: CCDS33 LVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKLEENNHKTEA 550 560 570 580 >>CCDS58841.1 ALCAM gene_id:214|Hs109|chr3 (570 aa) initn: 3313 init1: 3313 opt: 3320 Z-score: 2983.3 bits: 561.9 E(33420): 7.7e-160 Smith-Waterman score: 3697; 97.6% identity (97.8% similar) in 583 aa overlap (1-583:1-570) 10 20 30 40 50 60 pF1KE2 MESKGASSCRLLFCLLISATVFRPGLGWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 MESKGASSCRLLFCLLISATVFRPGLGWYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEK 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE2 PDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 PDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDN 70 80 90 100 110 120 130 140 150 160 170 180 pF1KE2 VFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 VFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPL 130 140 150 160 170 180 190 200 210 220 230 240 pF1KE2 EGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 EGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVF 190 200 210 220 230 240 250 260 270 280 290 300 pF1KE2 DIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 DIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTL 250 260 270 280 290 300 310 320 330 340 350 360 pF1KE2 TDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 TDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASR 310 320 330 340 350 360 370 380 390 400 410 420 pF1KE2 NATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 NATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKM 370 380 390 400 410 420 430 440 450 460 470 480 pF1KE2 TKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEEN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS58 TKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEEN 430 440 450 460 470 480 490 500 510 520 530 540 pF1KE2 VTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAKLIVGIVVGLLLAA :::::::::::::::::::::: .:::::::::::::::::::::::: CCDS58 VTLTCTAENQLERTVNSLNVSA-------------NENREKVNDQAKLIVGIVVGLLLAA 490 500 510 520 550 560 570 580 pF1KE2 LVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKLEENNHKTEA ::::::::::::::::::::::::::::::::::::::::::: CCDS58 LVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKLEENNHKTEA 530 540 550 560 570 >>CCDS31690.1 MCAM gene_id:4162|Hs109|chr11 (646 aa) initn: 222 init1: 140 opt: 453 Z-score: 416.4 bits: 87.2 E(33420): 7.3e-17 Smith-Waterman score: 645; 24.7% identity (59.8% similar) in 590 aa overlap (31-575:36-607) 10 20 30 40 50 pF1KE2 MESKGASSCRLLFCLLISATVFRPGLGWYTVNSAYGDTIIIPCRLDVPQ-NLMFGKWKYE :. :.: .. : :. : :: : CCDS31 LVCAFLLAACCCCPRVAGVPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSV 10 20 30 40 50 60 60 70 80 90 100 110 pF1KE2 KPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSE-NYTLSISNARISDEKRFVCMLVTE . . ... :.. .: . ::..::.:.. . ::..... .::. :.:. . CCDS31 HKEKRTLIFRVRQGQGQS----EPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQG-KR 70 80 90 100 110 120 120 130 140 150 160 170 pF1KE2 DNVFEAPTIVKVFKQPSKPEIVSKALFL--ETEQLKKLGDCISEDSYPDGNITWYRNGKV : ..:.: : .:.: . : . .... .... :.....:: .. ::.::. CCDS31 PRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRP 130 140 150 160 170 180 180 190 200 210 220 230 pF1KE2 LHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSE :. .. : : .. .. . :::. : :. . .: : . : : ..: :::.. .:. CCDS31 LKEEKNRVHIQSSQTVES-SGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESR 190 200 210 220 230 240 250 260 270 280 290 pF1KE2 QAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSN ... ..::::.: ..: : . .:::: . ..::..:::::. : . : . : .. CCDS31 EVTVPVFYPTEKVWLEV-EPVGMLKEGDRVEIRCLADGNPPPH---FSISKQNPSTREAE 240 250 260 270 280 290 300 310 320 330 340 pF1KE2 TYTLTD--------VRRNATGDYKCSLIDKKSMIASTA----ITVHYL-DLSLNPSGEVT : .: .:.. .: :.:. .: .::. . . :.:. :. ..:.. CCDS31 EETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAP-E 300 310 320 330 340 350 350 360 370 380 390 pF1KE2 RQIGDALPVSCTISASRNATVVWMKDNIR--LRSSP--SFSSLHYQDAGNYVCETALQEV :: :..: ..: .:.. :.... :. .: .. .:. . .:.: : ... . CCDS31 RQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSI 360 370 380 390 400 410 400 410 420 430 440 450 pF1KE2 EGLKKRESLTLIVEGKPQI--KMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVIN ::.. . ... . : : . : : .. .. :.. : :.:.:.:...:..: CCDS31 PGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTAS--- 420 430 440 450 460 470 460 470 480 490 500 pF1KE2 QTEESPYINGRYYS--KIIISPEENVT-LTCTAENQLERTVNSLNVSAISI----PE--- . ...: : : .....:: : . ::: :.: .... : . ... :. CCDS31 EQDQDPQ---RVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNT 480 490 500 510 520 510 520 530 540 550 pF1KE2 -----------HDEADEISDENR-EKVNDQAKLIVGIVVGLLLAALVAGVVYWLYMKKSK : .:. : : . . .... .::...: .:. :....:.:.:: ::.: CCDS31 TTGLSTSTASPHTRANSTSTERKLPEPESRGVVIVAVIVCILVLAVLGAVLYFLY-KKGK 530 540 550 560 570 580 560 570 580 pF1KE2 TASKHVNKDLGNMEENKKLEENNHKTEA .. .:. .. ..: : CCDS31 LPCRRSGKQEITLPPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH 590 600 610 620 630 640 583 residues in 1 query sequences 18921897 residues in 33420 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Tue Apr 23 11:12:16 2019 done: Tue Apr 23 11:12:17 2019 Total Scan time: 1.470 Total Display time: 0.040 Function used was FASTA [36.3.4 Apr, 2011]