Restriction map for
pF1KE2019
(Circular) (Six-base) MAPSORT of: pF1KE2019.seq Check: 8105 from: 1 to: 4901
pF1KE2019 4901 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 411 964 1939 411
Size: 553 975 3373
Fragments arranged by size:
3373 975 553
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4901
AclI AA'CG_TT
Cuts at: 4741 4741
Size: 4901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1826 2726 3158 3905 4173 1826
Size: 900 432 747 268 2554
Fragments arranged by size:
2554 900 747 432 268
AflIII A'CryG_T
Cuts at: 2253 3357 4334 2253
Size: 1104 977 2820
Fragments arranged by size:
2820 1104 977
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 378 649 693 1183 378
Size: 271 44 490 4096
Fragments arranged by size:
4096 490 271 44
AlwNI CAG_nnn'CTG
Cuts at: 2206 3773 2206
Size: 1567 3334
ApaI G_GGCC'C
Cuts at: 230 230
Size: 4901
ApaLI G'TGCA_C
Cuts at: 3671 3671
Size: 4901
ApoI r'AATT_y
Cuts at: 514 583 1905 4254 4510 514
Size: 69 1322 2349 256 905
Fragments arranged by size:
2349 1322 905 256 69
AseI AT'TA_AT
Cuts at: 20 995 20
Size: 975 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 223 1921 223
Size: 1698 3203
BamHI G'GATC_C
Cuts at: 1926 1926
Size: 4901
BanI G'GyrC_C
Cuts at: 1917 2331 2579 2614 1917
Size: 414 248 35 4204
Fragments arranged by size:
4204 414 248 35
BanII G_rGCy'C
Cuts at: 230 1915 2945 230
Size: 1685 1030 2186
Fragments arranged by size:
2186 1685 1030
BbeI G_GCGC'C
Cuts at: 2335 2583 2335
Size: 248 4653
BbsI GAAGACnn'nnnn_
Cuts at: 1792 1792
Size: 4901
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3560 4901 2799
Size: 761 1341 2799
Fragments arranged by size:
2799 1341 761
BclI T'GATC_A
Cuts at: 1770 2421 1770
Size: 651 4250
BfrBI ATG'CAT
Cuts at: 1557 1605 1659 1557
Size: 48 54 4799
Fragments arranged by size:
4799 54 48
BglII A'GATC_T
Cuts at: 2416 4453 2416
Size: 2037 2864
BlpI GC'TnA_GC
Cuts at: 1475 2013 1475
Size: 538 4363
Bme1580I G_kGCm'C
Cuts at: 230 840 2526 2619 3675 230
Size: 610 1686 93 1056 1456
Fragments arranged by size:
1686 1456 1056 610 93
BmrI ACTGGGnnnn_n'
Cuts at: 241 2113 2194 2529 4673 241
Size: 1872 81 335 2144 469
Fragments arranged by size:
2144 1872 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1209 1800 3268 1209
Size: 591 1468 2842
Fragments arranged by size:
2842 1468 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1115 838
Size: 277 4624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 939 2077 2956 3448 3746 939
Size: 1138 879 492 298 2094
Fragments arranged by size:
2094 1138 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4605 4605
Size: 4901
BsaAI yAC'GTr
Cuts at: 2254 2254
Size: 4901
BsaBI GATnn'nnATC
Cuts at: 6 2415 4452 6
Size: 2409 2037 455
Fragments arranged by size:
2409 2037 455
BsaHI Gr'CG_yC
Cuts at: 2332 2580 3282 4778 2332
Size: 248 702 1496 2455
Fragments arranged by size:
2455 1496 702 248
BsaWI w'CCGG_w
Cuts at: 659 2090 2611 3289 3563 3710 4121 659
Size: 1431 521 678 274 147 411 1439
Fragments arranged by size:
1439 1431 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 198 228 454 484 836 866 198
Size: 30 226 30 352 30 4233
Fragments arranged by size:
4233 352 226 30 30 30
BseYI C'CCAG_C
Cuts at: 1170 2326 3084 3661 4157 1170
Size: 1156 758 577 496 1914
Fragments arranged by size:
1914 1156 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1198 689
Size: 509 4392
BsiEI CG_ry'CG
Cuts at: 104 834 2443 2489 3697 104
Size: 730 1609 46 1208 1308
Fragments arranged by size:
1609 1308 1208 730 46
BsiHKAI G_wGCw'C
Cuts at: 1915 2693 2883 3675 1915
Size: 778 190 792 3141
Fragments arranged by size:
3141 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 230 840 1915 2526 2619 2693 2883 2945 3675
Size: 610 1075 611 93 74 190 62 730
Cuts at: 3675 230
Size: 1456
Fragments arranged by size:
1456 1075 730 611 610 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4121 4121
Size: 4901
BspHI T'CATG_A
Cuts at: 1773 4077 1773
Size: 2304 2597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 685 715 1951 2165 2467 2848 685
Size: 30 1236 214 302 381 2738
Fragments arranged by size:
2738 1236 381 302 214 30
BsrBI CCG'CTC
Cuts at: 50 830 3193 3247 4731 4896 50
Size: 780 2363 54 1484 165 55
Fragments arranged by size:
2363 1484 780 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2813 2813
Size: 4901
BsrFI r'CCGG_y
Cuts at: 137 2899 3080 3289 137
Size: 2762 181 209 1749
Fragments arranged by size:
2762 1749 209 181
BsrGI T'GTAC_A
Cuts at: 1823 1823
Size: 4901
BssSI C'ACGA_G
Cuts at: 3172 3530 3172
Size: 358 4543
BstAPI GCAn_nnn'nTGC
Cuts at: 730 2278 730
Size: 1548 3353
BstBI TT'CG_AA
Cuts at: 3262 3262
Size: 4901
BstXI CCAn_nnnn'nTGG
Cuts at: 1123 1123
Size: 4901
BstYI r'GATC_y
Cuts at: 1 341 1926 2393 2416 2751 2997 4009 4095
Size: 340 1585 467 23 335 246 1012 86
Cuts at: 4095 4107 4453 1
Size: 12 346 449
Fragments arranged by size:
1585 1012 467 449 346 340 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2851 3032 3195 2851
Size: 181 163 4557
Fragments arranged by size:
4557 181 163
BtsI GCAGTG_nn'
Cuts at: 350 827 838 1100 1469 2144 350
Size: 477 11 262 369 675 3107
Fragments arranged by size:
3107 675 477 369 262 11
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DraIII CAC_nnn'GTG
Cuts at: 1743 1743
Size: 4901
DrdI GACnn_nn'nnGTC
Cuts at: 2607 3465 2607
Size: 858 4043
EaeI y'GGCC_r
Cuts at: 717 831 1142 2486 2660 3051 3078 4423 717
Size: 114 311 1344 174 391 27 1345 1195
Fragments arranged by size:
1345 1344 1195 391 311 174 114 27
EagI C'GGCC_G
Cuts at: 831 2486 831
Size: 1655 3246
EarI CTCTTCn'nnn_
Cuts at: 1178 1802 2924 3134 1178
Size: 624 1122 210 2945
Fragments arranged by size:
2945 1122 624 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3109 3419 3565 4711 3109
Size: 310 146 1146 3299
Fragments arranged by size:
3299 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1913 1913
Size: 4901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1115 1826 2726 3158 3905 4173 838
Size: 277 711 900 432 747 268 1566
Fragments arranged by size:
1566 900 747 711 432 277 268
EcoNI CCTnn'n_nnAGG
Cuts at: 450 1005 450
Size: 555 4346
EcoO109I rG'GnC_Cy
Cuts at: 2040 2040
Size: 4901
EcoRI G'AATT_C
Cuts at: 1905 1905
Size: 4901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4901
FspI TGC'GCA
Cuts at: 2682 2682
Size: 4901
HaeII r_GCGC'y
Cuts at: 1782 2335 2583 3605 4584 1782
Size: 553 248 1022 979 2099
Fragments arranged by size:
2099 1022 979 553 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1755 1787 2743 2773 2775 2805 1755
Size: 32 956 30 2 30 3851
Fragments arranged by size:
3851 956 32 30 30 2
HincII GTy'rAC
Cuts at: 412 965 1131 1940 412
Size: 553 166 809 3373
Fragments arranged by size:
3373 809 553 166
KasI G'GCGC_C
Cuts at: 2331 2579 2331
Size: 248 4653
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4901
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3572 3756 3572
Size: 184 4717
MscI TGG'CCA
Cuts at: 719 1144 2662 719
Size: 425 1518 2958
Fragments arranged by size:
2958 1518 425
MslI CAynn'nnrTG
Cuts at: 872 1121 2890 3017 872
Size: 249 1769 127 2756
Fragments arranged by size:
2756 1769 249 127
MspA1I CmG'CkG
Cuts at: 633 2012 2326 2686 3699 3944 633
Size: 1379 314 360 1013 245 1590
Fragments arranged by size:
1590 1379 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3082 3082
Size: 4901
NarI GG'CG_CC
Cuts at: 2332 2580 2332
Size: 248 4653
NdeI CA'TA_TG
Cuts at: 438 438
Size: 4901
NgoMIV G'CCGG_C
Cuts at: 3080 3080
Size: 4901
NsiI A_TGCA'T
Cuts at: 1559 1607 1661 1559
Size: 48 54 4799
Fragments arranged by size:
4799 54 48
NspI r_CATG'y
Cuts at: 359 1605 1954 2454 3361 359
Size: 1246 349 500 907 1899
Fragments arranged by size:
1899 1246 907 500 349
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4901
PflMI CCAn_nnn'nTGG
Cuts at: 1743 1743
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4869
PspOMI G'GGCC_C
Cuts at: 226 226
Size: 4901
PstI C_TGCA'G
Cuts at: 1948 2633 1948
Size: 685 4216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 633 2326 2686 633
Size: 1693 360 2848
Fragments arranged by size:
2848 1693 360
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 1915 1915
Size: 4901
SalI G'TCGA_C
Cuts at: 410 963 1938 410
Size: 553 975 3373
Fragments arranged by size:
3373 975 553
SapI GCTCTTCn'nnn_
Cuts at: 1802 2924 3134 1802
Size: 1122 210 3569
Fragments arranged by size:
3569 1122 210
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4901
ScaI AGT'ACT
Cuts at: 4418 4418
Size: 4901
SexAI A'CCwGG_T
Cuts at: 1070 1070
Size: 4901
SfcI C'TryA_G
Cuts at: 33 1944 2629 3622 3813 33
Size: 1911 685 993 191 1121
Fragments arranged by size:
1911 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2333 2581 2333
Size: 248 4653
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SmaI CCC'GGG
Cuts at: 225 1923 225
Size: 1698 3203
SmlI C'TyrA_G
Cuts at: 918 2056 2971 3463 3725 918
Size: 1138 915 492 262 2094
Fragments arranged by size:
2094 1138 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4901
SphI G_CATG'C
Cuts at: 1605 1954 2454 1605
Size: 349 500 4052
Fragments arranged by size:
4052 500 349
StyI C'CwwG_G
Cuts at: 483 612 1028 2035 2389 483
Size: 129 416 1007 354 2995
Fragments arranged by size:
2995 1007 416 354 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 496 663 1162 1573 2484 2617 3076 3285 496
Size: 167 499 411 911 133 459 209 2112
Fragments arranged by size:
2112 911 499 459 411 209 167 133
TatI w'GTAC_w
Cuts at: 476 905 1823 4416 476
Size: 429 918 2593 961
Fragments arranged by size:
2593 961 918 429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 414 446 454 486 1569 1601 4418
Size: 32 184 32 8 32 1083 32 2817
Cuts at: 4418 4450 198
Size: 32 649
Fragments arranged by size:
2817 1083 649 184 32 32 32 32
32 8
Tth111I GACn'n_nGTC
Cuts at: 2698 2698
Size: 4901
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 464 1107 2396 464
Size: 643 1289 2969
Fragments arranged by size:
2969 1289 643
XmaI C'CCGG_G
Cuts at: 223 1921 223
Size: 1698 3203
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI