Restriction map for
pF1KE2032
(Circular) (Six-base) MAPSORT of: pF1KE2032.seq Check: 8491 from: 1 to: 4979
pF1KE2032 4979 bp
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1230 1668 1230
Size: 438 4541
AccI GT'mk_AC
Cuts at: 2017 2017
Size: 4979
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 1649 4819 1649
Size: 3170 1809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1512 1531 1648 1954 2804 3236 3983 4251 1512
Size: 19 117 306 850 432 747 268 2240
Fragments arranged by size:
2240 850 747 432 306 268 117 19
AflIII A'CryG_T
Cuts at: 1748 1920 2331 3435 4412 1748
Size: 172 411 1104 977 2315
Fragments arranged by size:
2315 1104 977 411 172
AgeI A'CCGG_T
Cuts at: 3367 3367
Size: 4979
AhdI GACnn_n'nnGTC
Cuts at: 1822 1822
Size: 4979
AleI CACnn'nnGTG
Cuts at: 1921 1921
Size: 4979
AlwNI CAG_nnn'CTG
Cuts at: 1272 2284 3851 1272
Size: 1012 1567 2400
Fragments arranged by size:
2400 1567 1012
ApaLI G'TGCA_C
Cuts at: 172 3749 172
Size: 3577 1402
ApoI r'AATT_y
Cuts at: 570 1983 4332 4588 570
Size: 1413 2349 256 961
Fragments arranged by size:
2349 1413 961 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 395 1098 1999 395
Size: 703 901 3375
Fragments arranged by size:
3375 901 703
BamHI G'GATC_C
Cuts at: 1730 2004 1730
Size: 274 4705
BanI G'GyrC_C
Cuts at: 776 1995 2409 2657 2692 776
Size: 1219 414 248 35 3063
Fragments arranged by size:
3063 1219 414 248 35
BanII G_rGCy'C
Cuts at: 803 1172 1210 1837 1993 3023 803
Size: 369 38 627 156 1030 2759
Fragments arranged by size:
2759 1030 627 369 156 38
BbeI G_GCGC'C
Cuts at: 780 2413 2661 780
Size: 1633 248 3098
Fragments arranged by size:
3098 1633 248
BbvCI CC'TCA_GC
Cuts at: 200 434 200
Size: 234 4745
BciVI GTATCCnnnnn_n'
Cuts at: 1588 1937 2877 3638 4979 1588
Size: 349 940 761 1341 1588
Fragments arranged by size:
1588 1341 940 761 349
BclI T'GATC_A
Cuts at: 1959 2499 1959
Size: 540 4439
BglI GCCn_nnn'nGGC
Cuts at: 322 322
Size: 4979
BglII A'GATC_T
Cuts at: 1445 2494 4531 1445
Size: 1049 2037 1893
Fragments arranged by size:
2037 1893 1049
BlpI GC'TnA_GC
Cuts at: 2091 2091
Size: 4979
Bme1580I G_kGCm'C
Cuts at: 176 2604 2697 3753 176
Size: 2428 93 1056 1402
Fragments arranged by size:
2428 1402 1056 93
BmgBI CAC'GTC
Cuts at: 117 299 1637 117
Size: 182 1338 3459
Fragments arranged by size:
3459 1338 182
BmrI ACTGGGnnnn_n'
Cuts at: 1661 2191 2272 2607 4751 1661
Size: 530 81 335 2144 1889
Fragments arranged by size:
2144 1889 530 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 410 3346 410
Size: 2936 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 295 349 696 1168 1570 205
Size: 90 54 347 472 402 3614
Fragments arranged by size:
3614 472 402 347 90 54
Bpu10I CC'TnA_GC
Cuts at: 200 434 841 200
Size: 234 407 4338
Fragments arranged by size:
4338 407 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 3034 3526 3824 2155
Size: 879 492 298 3310
Fragments arranged by size:
3310 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4683 4683
Size: 4979
BsaAI yAC'GTr
Cuts at: 2332 2332
Size: 4979
BsaBI GATnn'nnATC
Cuts at: 6 1450 1735 2493 4530 6
Size: 1444 285 758 2037 455
Fragments arranged by size:
2037 1444 758 455 285
BsaHI Gr'CG_yC
Cuts at: 391 777 2410 2658 3360 4856 391
Size: 386 1633 248 702 1496 514
Fragments arranged by size:
1633 1496 702 514 386 248
BsaWI w'CCGG_w
Cuts at: 1253 2168 2689 3367 3641 3788 4199 1253
Size: 915 521 678 274 147 411 2033
Fragments arranged by size:
2033 915 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 283 298 313 785 815 907 937 1261
Size: 15 15 15 472 30 92 30 324
Cuts at: 1261 1291 268
Size: 30 3956
Fragments arranged by size:
3956 472 324 92 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 188 268 307 325 519 651 928 997 1009
Size: 80 39 18 194 132 277 69 12
Cuts at: 1009 1012 188
Size: 3 4155
Fragments arranged by size:
4155 277 194 132 80 69 39 18
12 3
BseYI C'CCAG_C
Cuts at: 1304 1616 2404 3162 3739 4235 1304
Size: 312 788 758 577 496 2048
Fragments arranged by size:
2048 788 758 577 496 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 892 321
Size: 571 4408
BsiEI CG_ry'CG
Cuts at: 104 214 1708 2521 2567 3775 104
Size: 110 1494 813 46 1208 1308
Fragments arranged by size:
1494 1308 1208 813 110 46
BsiHKAI G_wGCw'C
Cuts at: 176 1172 1993 2771 2961 3753 176
Size: 996 821 778 190 792 1402
Fragments arranged by size:
1402 996 821 792 778 190
BsmI GAATG_Cn'
Cuts at: 652 652
Size: 4979
BsmBI CGTCTCn'nnnn_
Cuts at: 289 1561 1627 1875 289
Size: 1272 66 248 3393
Fragments arranged by size:
3393 1272 248 66
Bsp1286I G_dGCh'C
Cuts at: 176 803 1172 1210 1837 1993 2604 2697 2771
Size: 627 369 38 627 156 611 93 74
Cuts at: 2771 2961 3023 3753 176
Size: 190 62 730 1402
Fragments arranged by size:
1402 730 627 627 611 369 190 156
93 74 62 38
BspEI T'CCGG_A
Cuts at: 1253 4199 1253
Size: 2946 2033
BspHI T'CATG_A
Cuts at: 1826 4155 1826
Size: 2329 2650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1230 1668 1697 2029 2243 2545 2926 1230
Size: 438 29 332 214 302 381 3283
Fragments arranged by size:
3283 438 381 332 302 214 29
BsrBI CCG'CTC
Cuts at: 50 1704 3271 3325 4809 4974 50
Size: 1654 1567 54 1484 165 55
Fragments arranged by size:
1654 1567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 2891 1493
Size: 1398 3581
BsrFI r'CCGG_y
Cuts at: 1707 2977 3158 3367 1707
Size: 1270 181 209 3319
Fragments arranged by size:
3319 1270 209 181
BssSI C'ACGA_G
Cuts at: 3250 3608 3250
Size: 358 4621
BstAPI GCAn_nnn'nTGC
Cuts at: 491 1245 2356 491
Size: 754 1111 3114
Fragments arranged by size:
3114 1111 754
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstEII G'GTnAC_C
Cuts at: 786 786
Size: 4979
BstYI r'GATC_y
Cuts at: 1 1445 1730 2004 2471 2494 2829 3075 4087
Size: 1444 285 274 467 23 335 246 1012
Cuts at: 4087 4173 4185 4531 1
Size: 86 12 346 449
Fragments arranged by size:
1444 1012 467 449 346 335 285 274
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 338 599 989 107
Size: 231 261 390 4097
Fragments arranged by size:
4097 390 261 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 703 2929 3110 3273 703
Size: 2226 181 163 2409
Fragments arranged by size:
2409 2226 181 163
BtsI GCAGTG_nn'
Cuts at: 748 1323 2222 748
Size: 575 899 3505
Fragments arranged by size:
3505 899 575
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DraIII CAC_nnn'GTG
Cuts at: 1548 1548
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 1306 2685 3543 1306
Size: 1379 858 2742
Fragments arranged by size:
2742 1379 858
EaeI y'GGCC_r
Cuts at: 666 935 1705 2564 2738 3129 3156 4501 666
Size: 269 770 859 174 391 27 1345 1144
Fragments arranged by size:
1345 1144 859 770 391 269 174 27
EagI C'GGCC_G
Cuts at: 1705 2564 1705
Size: 859 4120
EarI CTCTTCn'nnn_
Cuts at: 1252 1825 3002 3212 1252
Size: 573 1177 210 3019
Fragments arranged by size:
3019 1177 573 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1918 3187 3497 3643 4789 1918
Size: 1269 310 146 1146 2108
Fragments arranged by size:
2108 1269 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1170 1991 1170
Size: 821 4158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 295 349 696 1168 1512 1531 1570 1648
Size: 90 54 347 472 344 19 39 78
Cuts at: 1648 1954 2804 3236 3983 4251 205
Size: 306 850 432 747 268 933
Fragments arranged by size:
933 850 747 472 432 347 344 306
268 90 78 54 39 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1775 1775
Size: 4979
EcoO109I rG'GnC_Cy
Cuts at: 604 837 1088 1308 1816 2118 604
Size: 233 251 220 508 302 3465
Fragments arranged by size:
3465 508 302 251 233 220
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
EcoRV GAT'ATC
Cuts at: 1430 1430
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1854 2998 1854
Size: 1144 3835
FspI TGC'GCA
Cuts at: 262 1381 2760 262
Size: 1119 1379 2481
Fragments arranged by size:
2481 1379 1119
FspAI rTGC'GCAy
Cuts at: 1381 1381
Size: 4979
HaeII r_GCGC'y
Cuts at: 780 2413 2661 3683 4662 780
Size: 1633 248 1022 979 1097
Fragments arranged by size:
1633 1097 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 847 879 1009 1041 1126 1158 1808 1840 2821
Size: 32 130 32 85 32 650 32 981
Cuts at: 2821 2851 2853 2883 847
Size: 30 2 30 2943
Fragments arranged by size:
2943 981 650 130 85 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2018 2018
Size: 4979
HindIII A'AGCT_T
Cuts at: 403 403
Size: 4979
KasI G'GCGC_C
Cuts at: 776 2409 2657 776
Size: 1633 248 3098
Fragments arranged by size:
3098 1633 248
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1418 3650 3834 1418
Size: 2232 184 2563
Fragments arranged by size:
2563 2232 184
MscI TGG'CCA
Cuts at: 668 2740 668
Size: 2072 2907
MslI CAynn'nnrTG
Cuts at: 1921 2968 3095 1921
Size: 1047 127 3805
Fragments arranged by size:
3805 1047 127
MspA1I CmG'CkG
Cuts at: 132 225 868 876 1304 1850 2090 2404 2764
Size: 93 643 8 428 546 240 314 360
Cuts at: 2764 3777 4022 132
Size: 1013 245 1089
Fragments arranged by size:
1089 1013 643 546 428 360 314 245
240 93 8
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 4979
NarI GG'CG_CC
Cuts at: 777 2410 2658 777
Size: 1633 248 3098
Fragments arranged by size:
3098 1633 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4979
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 4979
NspI r_CATG'y
Cuts at: 187 416 466 1230 1752 1924 2032 2532 3439
Size: 229 50 764 522 172 108 500 907
Cuts at: 3439 187
Size: 1727
Fragments arranged by size:
1727 907 764 522 500 229 172 108
50
PasI CC'CwG_GG
Cuts at: 1343 1343
Size: 4979
PciI A'CATG_T
Cuts at: 1748 1920 3435 1748
Size: 172 1515 3292
Fragments arranged by size:
3292 1515 172
PfoI T'CCnGG_A
Cuts at: 1172 1172
Size: 4979
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4947
PpuMI rG'GwC_Cy
Cuts at: 604 1088 1308 1816 604
Size: 484 220 508 3767
Fragments arranged by size:
3767 508 484 220
PstI C_TGCA'G
Cuts at: 1679 2026 2711 1679
Size: 347 685 3947
Fragments arranged by size:
3947 685 347
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 132 868 876 2404 2764 132
Size: 736 8 1528 360 2347
Fragments arranged by size:
2347 1528 736 360 8
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1172 1993 1172
Size: 821 4158
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SanDI GG'GwC_CC
Cuts at: 604 1816 604
Size: 1212 3767
SapI GCTCTTCn'nnn_
Cuts at: 1825 3002 3212 1825
Size: 1177 210 3592
Fragments arranged by size:
3592 1177 210
SbfI CC_TGCA'GG
Cuts at: 1679 2026 1679
Size: 347 4632
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SexAI A'CCwGG_T
Cuts at: 1402 1402
Size: 4979
SfcI C'TryA_G
Cuts at: 33 562 620 683 1675 2022 2707 3700 3891
Size: 529 58 63 992 347 685 993 191
Cuts at: 3891 33
Size: 1121
Fragments arranged by size:
1121 993 992 685 529 347 191 63
58
SfoI GGC'GCC
Cuts at: 778 2411 2659 778
Size: 1633 248 3098
Fragments arranged by size:
3098 1633 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 2001 2001
Size: 4979
SmlI C'TyrA_G
Cuts at: 395 2134 3049 3541 3803 395
Size: 1739 915 492 262 1571
Fragments arranged by size:
1739 1571 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4479
StuI AGG'CCT
Cuts at: 316 316
Size: 4979
StyI C'CwwG_G
Cuts at: 107 833 1234 2113 2467 107
Size: 726 401 879 354 2619
Fragments arranged by size:
2619 879 726 401 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2562 2695 3154 3363 2562
Size: 133 459 209 4178
Fragments arranged by size:
4178 459 209 133
TatI w'GTAC_w
Cuts at: 365 4494 365
Size: 4129 850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 268 283 300 315 531 563 785 817 907
Size: 15 17 15 216 32 222 32 90
Cuts at: 907 939 1261 1293 1910 1942 4496 4528 268
Size: 32 322 32 617 32 2554 32 719
Fragments arranged by size:
2554 719 617 322 222 216 90 32
32 32 32 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 2776 2776
Size: 4979
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 246 2474 246
Size: 2228 2751
XhoI C'TCGA_G
Cuts at: 395 395
Size: 4979
XmaI C'CCGG_G
Cuts at: 1999 1999
Size: 4979
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI FseI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI