Restriction map for
pF1KE2061
(Circular) (Six-base) MAPSORT of: pF1KE2061.seq Check: 122 from: 1 to: 5228
pF1KE2061 5228 bp
With 182 enzymes: SgfI *
January 30, 2017 10:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 390 390
Size: 5228
AatII G_ACGT'C
Cuts at: 1096 2001 1096
Size: 905 4323
AccI GT'mk_AC
Cuts at: 764 2266 764
Size: 1502 3726
Acc65I G'GTAC_C
Cuts at: 2244 2244
Size: 5228
AclI AA'CG_TT
Cuts at: 5068 5068
Size: 5228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 660 661 3053 3485 4232 4500 375
Size: 285 1 2392 432 747 268 1103
Fragments arranged by size:
2392 1103 747 432 285 268 1
AfeI AGC'GCT
Cuts at: 1229 1229
Size: 5228
AflIII A'CryG_T
Cuts at: 1181 2580 3684 4661 1181
Size: 1399 1104 977 1748
Fragments arranged by size:
1748 1399 1104 977
AgeI A'CCGG_T
Cuts at: 3616 3616
Size: 5228
AhdI GACnn_n'nnGTC
Cuts at: 1688 1688
Size: 5228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1633 1633
Size: 5228
AlwNI CAG_nnn'CTG
Cuts at: 1359 2103 2533 4100 1359
Size: 744 430 1567 2487
Fragments arranged by size:
2487 1567 744 430
ApaI G_GGCC'C
Cuts at: 1431 1472 1786 1848 1876 1990 1431
Size: 41 314 62 28 114 4669
Fragments arranged by size:
4669 314 114 62 41 28
ApaLI G'TGCA_C
Cuts at: 3998 3998
Size: 5228
ApoI r'AATT_y
Cuts at: 547 835 2232 4581 4837 547
Size: 288 1397 2349 256 938
Fragments arranged by size:
2349 1397 938 288 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5228
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
AvaI C'yCGr_G
Cuts at: 244 310 318 1726 1983 2248 244
Size: 66 8 1408 257 265 3224
Fragments arranged by size:
3224 1408 265 257 66 8
BamHI G'GATC_C
Cuts at: 2253 2253
Size: 5228
BanI G'GyrC_C
Cuts at: 122 214 1731 2244 2658 2906 2941 122
Size: 92 1517 513 414 248 35 2409
Fragments arranged by size:
2409 1517 513 414 248 92 35
BanII G_rGCy'C
Cuts at: 1025 1334 1402 1431 1472 1786 1848 1876 1990
Size: 309 68 29 41 314 62 28 114
Cuts at: 1990 2242 3272 1025
Size: 252 1030 2981
Fragments arranged by size:
2981 1030 314 309 252 114 68 62
41 29 28
BbeI G_GCGC'C
Cuts at: 126 1735 2662 2910 126
Size: 1609 927 248 2444
Fragments arranged by size:
2444 1609 927 248
BbsI GAAGACnn'nnnn_
Cuts at: 1120 1316 1120
Size: 196 5032
BbvCI CC'TCA_GC
Cuts at: 1644 1644
Size: 5228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1951 1985 1951
Size: 34 5194
BciVI GTATCCnnnnn_n'
Cuts at: 3126 3887 5228 3126
Size: 761 1341 3126
Fragments arranged by size:
3126 1341 761
BclI T'GATC_A
Cuts at: 849 2748 849
Size: 1899 3329
BglI GCCn_nnn'nGGC
Cuts at: 286 295 1467 1781 1843 1901 1924 286
Size: 9 1172 314 62 58 23 3590
Fragments arranged by size:
3590 1172 314 62 58 23 9
BglII A'GATC_T
Cuts at: 2743 4780 2743
Size: 2037 3191
BlpI GC'TnA_GC
Cuts at: 883 1478 2340 883
Size: 595 862 3771
Fragments arranged by size:
3771 862 595
Bme1580I G_kGCm'C
Cuts at: 219 923 1431 1472 1786 1848 1876 1940 1990
Size: 704 508 41 314 62 28 64 50
Cuts at: 1990 2853 2946 4002 219
Size: 863 93 1056 1445
Fragments arranged by size:
1445 1056 863 704 508 314 93 64
62 50 41 28
BmgBI CAC'GTC
Cuts at: 1047 2037 1047
Size: 990 4238
BmrI ACTGGGnnnn_n'
Cuts at: 492 734 832 1955 2091 2440 2521 2856 5000
Size: 242 98 1123 136 349 81 335 2144
Cuts at: 5000 492
Size: 720
Fragments arranged by size:
2144 1123 720 349 335 242 136 98
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 3595 285
Size: 3310 1918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 1159 1721 429
Size: 730 562 3936
Fragments arranged by size:
3936 730 562
Bpu10I CC'TnA_GC
Cuts at: 1352 1644 2152 1352
Size: 292 508 4428
Fragments arranged by size:
4428 508 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2404 3283 3775 4073 2404
Size: 879 492 298 3559
Fragments arranged by size:
3559 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 641 2170 4932 641
Size: 1529 2762 937
Fragments arranged by size:
2762 1529 937
BsaAI yAC'GTr
Cuts at: 1184 2581 1184
Size: 1397 3831
BsaBI GATnn'nnATC
Cuts at: 6 2742 4779 6
Size: 2736 2037 455
Fragments arranged by size:
2736 2037 455
BsaHI Gr'CG_yC
Cuts at: 123 1093 1732 1998 2659 2907 3609 5105 123
Size: 970 639 266 661 248 702 1496 246
Fragments arranged by size:
1496 970 702 661 639 266 248 246
BsaWI w'CCGG_w
Cuts at: 2417 2938 3616 3890 4037 4448 2417
Size: 521 678 274 147 411 3197
Fragments arranged by size:
3197 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 282 1454 1529 1602 1622 1652 1769 1896 1975
Size: 1172 75 73 20 30 117 127 79
Cuts at: 1975 2026 2081 2146 2653 3411 3988 4484 282
Size: 51 55 65 507 758 577 496 1026
Fragments arranged by size:
1172 1026 758 577 507 496 127 117
79 75 73 65 55 51 30 20
BsiEI CG_ry'CG
Cuts at: 104 299 2770 2816 4024 104
Size: 195 2471 46 1208 1308
Fragments arranged by size:
2471 1308 1208 195 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1334 2242 3020 3210 4002 1189
Size: 145 908 778 190 792 2415
Fragments arranged by size:
2415 908 792 778 190 145
BsmI GAATG_Cn'
Cuts at: 756 756
Size: 5228
BsmBI CGTCTCn'nnnn_
Cuts at: 1769 1818 1769
Size: 49 5179
Bsp1286I G_dGCh'C
Cuts at: 219 923 1025 1189 1334 1402 1431 1472 1786
Size: 704 102 164 145 68 29 41 314
Cuts at: 1786 1848 1876 1940 1990 2242 2853 2946 3020
Size: 62 28 64 50 252 611 93 74
Cuts at: 3020 3210 3272 4002 219
Size: 190 62 730 1445
Fragments arranged by size:
1445 730 704 611 314 252 190 164
145 102 93 74 68 64 62 62
50 41 29 28
BspEI T'CCGG_A
Cuts at: 4448 4448
Size: 5228
BspHI T'CATG_A
Cuts at: 4404 4404
Size: 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 125 188 390 2278 2492 2794 3175 125
Size: 63 202 1888 214 302 381 2178
Fragments arranged by size:
2178 1888 381 302 214 202 63
BsrBI CCG'CTC
Cuts at: 50 1287 3520 3574 5058 5223 50
Size: 1237 2233 54 1484 165 55
Fragments arranged by size:
2233 1484 1237 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1546 3140 1546
Size: 1594 3634
BsrFI r'CCGG_y
Cuts at: 1900 3226 3407 3616 1900
Size: 1326 181 209 3512
Fragments arranged by size:
3512 1326 209 181
BssSI C'ACGA_G
Cuts at: 1333 3499 3857 1333
Size: 2166 358 2704
Fragments arranged by size:
2704 2166 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2605 2605
Size: 5228
BstBI TT'CG_AA
Cuts at: 3589 3589
Size: 5228
BstEII G'GTnAC_C
Cuts at: 1294 1294
Size: 5228
BstXI CCAn_nnnn'nTGG
Cuts at: 580 1063 580
Size: 483 4745
BstYI r'GATC_y
Cuts at: 1 509 2253 2720 2743 3078 3324 4336 4422
Size: 508 1744 467 23 335 246 1012 86
Cuts at: 4422 4434 4780 1
Size: 12 346 449
Fragments arranged by size:
1744 1012 508 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2191 2191
Size: 5228
BtgI C'CryG_G
Cuts at: 368 429 513 902 1404 368
Size: 61 84 389 502 4192
Fragments arranged by size:
4192 502 389 84 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 963 1147 3178 3359 3522 963
Size: 184 2031 181 163 2669
Fragments arranged by size:
2669 2031 184 181 163
BtsI GCAGTG_nn'
Cuts at: 254 1013 1226 2471 254
Size: 759 213 1245 3011
Fragments arranged by size:
3011 1245 759 213
ClaI AT'CG_AT
Cuts at: 1031 1031
Size: 5228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 971 1006 971
Size: 35 5193
DraI TTT'AAA
Cuts at: 2227 2227
Size: 5228
DrdI GACnn_nn'nnGTC
Cuts at: 1051 1098 2934 3792 1051
Size: 47 1836 858 2487
Fragments arranged by size:
2487 1836 858 47
EaeI y'GGCC_r
Cuts at: 149 296 1407 1708 2813 2987 3378 3405 4750
Size: 147 1111 301 1105 174 391 27 1345
Cuts at: 4750 149
Size: 627
Fragments arranged by size:
1345 1111 1105 627 391 301 174 147
27
EagI C'GGCC_G
Cuts at: 296 2813 296
Size: 2517 2711
EarI CTCTTCn'nnn_
Cuts at: 2218 3251 3461 2218
Size: 1033 210 3985
Fragments arranged by size:
3985 1033 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 652 1817 3436 3746 3892 5038 652
Size: 1165 1619 310 146 1146 842
Fragments arranged by size:
1619 1165 1146 842 310 146
EcoICRI GAG'CTC
Cuts at: 1332 2240 1332
Size: 908 4320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 429 660 661 1159 1721 3053 3485 4232
Size: 54 231 1 498 562 1332 432 747
Cuts at: 4232 4500 375
Size: 268 1103
Fragments arranged by size:
1332 1103 747 562 498 432 268 231
54 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1086 2155 1086
Size: 1069 4159
EcoO109I rG'GnC_Cy
Cuts at: 266 314 1356 1427 1428 1468 1469 1525 1540
Size: 48 1042 71 1 40 1 56 15
Cuts at: 1540 1723 1783 1844 1845 1872 1930 1987 2068
Size: 183 60 61 1 27 58 57 81
Cuts at: 2068 2367 266
Size: 299 3127
Fragments arranged by size:
3127 1042 299 183 81 71 61 60
58 57 56 48 40 27 15 1
1 1
EcoRI G'AATT_C
Cuts at: 547 835 2232 547
Size: 288 1397 3543
Fragments arranged by size:
3543 1397 288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 5228
FspI TGC'GCA
Cuts at: 1218 3009 1218
Size: 1791 3437
HaeII r_GCGC'y
Cuts at: 126 1231 1536 1560 1629 1651 1735 1776 1869
Size: 1105 305 24 69 22 84 41 93
Cuts at: 1869 2662 2910 3932 4911 126
Size: 793 248 1022 979 443
Fragments arranged by size:
1105 1022 979 793 443 305 248 93
84 69 41 24 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1171 1196 1203 1228 1674 1706 3070 3100 3102
Size: 25 7 25 446 32 1364 30 2
Cuts at: 3102 3132 1171
Size: 30 3267
Fragments arranged by size:
3267 1364 446 32 30 30 25 25
7 2
HincII GTy'rAC
Cuts at: 747 954 1341 2267 747
Size: 207 387 926 3708
Fragments arranged by size:
3708 926 387 207
HpaI GTT'AAC
Cuts at: 747 747
Size: 5228
KasI G'GCGC_C
Cuts at: 122 1731 2658 2906 122
Size: 1609 927 248 2444
Fragments arranged by size:
2444 1609 927 248
KpnI G_GTAC'C
Cuts at: 2248 2248
Size: 5228
MluI A'CGCG_T
Cuts at: 4661 4661
Size: 5228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 113 374 2243 3899 4083 113
Size: 261 1869 1656 184 1258
Fragments arranged by size:
1869 1656 1258 261 184
MscI TGG'CCA
Cuts at: 151 1409 2989 151
Size: 1258 1580 2390
Fragments arranged by size:
2390 1580 1258
MslI CAynn'nnrTG
Cuts at: 578 1264 1304 3217 3344 578
Size: 686 40 1913 127 2462
Fragments arranged by size:
2462 1913 686 127 40
MspA1I CmG'CkG
Cuts at: 286 382 431 1377 1606 1979 1993 2013 2059
Size: 96 49 946 229 373 14 20 46
Cuts at: 2059 2339 2653 3013 4026 4271 286
Size: 280 314 360 1013 245 1243
Fragments arranged by size:
1243 1013 946 373 360 314 280 245
229 96 49 46 20 14
NaeI GCC'GGC
Cuts at: 1902 3409 1902
Size: 1507 3721
NarI GG'CG_CC
Cuts at: 123 1732 2659 2907 123
Size: 1609 927 248 2444
Fragments arranged by size:
2444 1609 927 248
NcoI C'CATG_G
Cuts at: 513 1404 513
Size: 891 4337
NgoMIV G'CCGG_C
Cuts at: 1900 3407 1900
Size: 1507 3721
NspI r_CATG'y
Cuts at: 2281 2781 3688 2281
Size: 500 907 3821
Fragments arranged by size:
3821 907 500
PasI CC'CwG_GG
Cuts at: 785 1423 1686 785
Size: 638 263 4327
Fragments arranged by size:
4327 638 263
PciI A'CATG_T
Cuts at: 3684 3684
Size: 5228
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 5228
PmeI GTTT'AAAC
Cuts at: 2227 2227
Size: 5228
PmlI CAC'GTG
Cuts at: 1184 1184
Size: 5228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 4367
Size: 32 5196
PpuMI rG'GwC_Cy
Cuts at: 266 314 1540 1930 2068 266
Size: 48 1226 390 138 3426
Fragments arranged by size:
3426 1226 390 138 48
PshAI GACnn'nnGTC
Cuts at: 1810 1810
Size: 5228
PspOMI G'GGCC_C
Cuts at: 1427 1468 1782 1844 1872 1986 1427
Size: 41 314 62 28 114 4669
Fragments arranged by size:
4669 314 114 62 41 28
PstI C_TGCA'G
Cuts at: 185 725 2064 2275 2960 185
Size: 540 1339 211 685 2453
Fragments arranged by size:
2453 1339 685 540 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5228
PvuII CAG'CTG
Cuts at: 382 1606 2013 2653 3013 382
Size: 1224 407 640 360 2597
Fragments arranged by size:
2597 1224 640 407 360
RsrII CG'GwC_CG
Cuts at: 3423 3423
Size: 5228
SacI G_AGCT'C
Cuts at: 1334 2242 1334
Size: 908 4320
SacII CC_GC'GG
Cuts at: 432 432
Size: 5228
SalI G'TCGA_C
Cuts at: 2265 2265
Size: 5228
SanDI GG'GwC_CC
Cuts at: 1540 1930 1540
Size: 390 4838
SapI GCTCTTCn'nnn_
Cuts at: 3251 3461 3251
Size: 210 5018
SbfI CC_TGCA'GG
Cuts at: 2275 2275
Size: 5228
ScaI AGT'ACT
Cuts at: 4745 4745
Size: 5228
SfcI C'TryA_G
Cuts at: 33 181 721 2060 2271 2956 3949 4140 33
Size: 148 540 1339 211 685 993 191 1121
Fragments arranged by size:
1339 1121 993 685 540 211 191 148
SfiI GGCCn_nnn'nGGCC
Cuts at: 295 1843 1901 295
Size: 1548 58 3622
Fragments arranged by size:
3622 1548 58
SfoI GGC'GCC
Cuts at: 124 1733 2660 2908 124
Size: 1609 927 248 2444
Fragments arranged by size:
2444 1609 927 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
SmaI CCC'GGG
Cuts at: 246 312 1728 1985 2250 246
Size: 66 1416 257 265 3224
Fragments arranged by size:
3224 1416 265 257 66
SmlI C'TyrA_G
Cuts at: 2383 3298 3790 4052 2383
Size: 915 492 262 3559
Fragments arranged by size:
3559 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5228
SphI G_CATG'C
Cuts at: 2281 2781 2281
Size: 500 4728
SrfI GCCC'GGGC
Cuts at: 1985 1985
Size: 5228
StuI AGG'CCT
Cuts at: 1081 1592 1837 1081
Size: 511 245 4472
Fragments arranged by size:
4472 511 245
StyI C'CwwG_G
Cuts at: 513 1404 1436 1593 2362 2716 513
Size: 891 32 157 769 354 3025
Fragments arranged by size:
3025 891 769 354 157 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 777 1164 1968 2811 2944 3403 3612 777
Size: 387 804 843 133 459 209 2393
Fragments arranged by size:
2393 843 804 459 387 209 133
TatI w'GTAC_w
Cuts at: 889 4743 889
Size: 3854 1374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 225 257 578 610 4745 4777 225
Size: 32 321 32 4135 32 676
Fragments arranged by size:
4135 676 321 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1416 3025 1416
Size: 1609 3619
XbaI T'CTAG_A
Cuts at: 2259 2259
Size: 5228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 580 2010 2723 453
Size: 127 1430 713 2958
Fragments arranged by size:
2958 1430 713 127
XmaI C'CCGG_G
Cuts at: 244 310 1726 1983 2248 244
Size: 66 1416 257 265 3224
Fragments arranged by size:
3224 1416 265 257 66
XmnI GAAnn'nnTTC
Cuts at: 608 2200 608
Size: 1592 3636
ZraI GAC'GTC
Cuts at: 1094 1999 1094
Size: 905 4323
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AleI AloI AscI AvrII BaeI BfrBI BmtI
BsaXI BseRI BsgI BsiWI BsrGI BssHII BstZ17I DraIII
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PsiI PspXI PsrI SexAI
SgrAI SnaBI SspI SwaI XhoI