(Circular) (Six-base) MAPSORT of: pF1KE2062.seq Check: 2661 from: 1 to: 5576 pF1KE2062 5576 bp With 182 enzymes: SgfI * July 8, 2010 13:19 .. AccI GT'mk_AC Cuts at: 446 2144 2614 446 Size: 1698 470 3408 Fragments arranged by size: 3408 1698 470 Acc65I G'GTAC_C Cuts at: 2592 2592 Size: 5576 AclI AA'CG_TT Cuts at: 2353 5416 2353 Size: 3063 2513 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 165 1317 2275 2465 3401 3833 4580 4848 165 Size: 1152 958 190 936 432 747 268 893 Fragments arranged by size: 1152 958 936 893 747 432 268 190 AflIII A'CryG_T Cuts at: 833 2928 4032 5009 833 Size: 2095 1104 977 1400 Fragments arranged by size: 2095 1400 1104 977 AgeI A'CCGG_T Cuts at: 3964 3964 Size: 5576 AhdI GACnn_n'nnGTC Cuts at: 1917 1917 Size: 5576 AlwNI CAG_nnn'CTG Cuts at: 1195 2881 4448 1195 Size: 1686 1567 2323 Fragments arranged by size: 2323 1686 1567 ApaI G_GGCC'C Cuts at: 350 1016 350 Size: 666 4910 ApaLI G'TGCA_C Cuts at: 337 1351 4346 337 Size: 1014 2995 1567 Fragments arranged by size: 2995 1567 1014 ApoI r'AATT_y Cuts at: 2384 2580 4929 5185 2384 Size: 196 2349 256 2775 Fragments arranged by size: 2775 2349 256 196 AseI AT'TA_AT Cuts at: 20 20 Size: 5576 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5576 AvaI C'yCGr_G Cuts at: 1852 2596 1852 Size: 744 4832 BamHI G'GATC_C Cuts at: 2601 2601 Size: 5576 BanI G'GyrC_C Cuts at: 394 2592 3006 3254 3289 394 Size: 2198 414 248 35 2681 Fragments arranged by size: 2681 2198 414 248 35 BanII G_rGCy'C Cuts at: 350 1016 1842 2212 2590 3620 350 Size: 666 826 370 378 1030 2306 Fragments arranged by size: 2306 1030 826 666 378 370 BbeI G_GCGC'C Cuts at: 398 3010 3258 398 Size: 2612 248 2716 Fragments arranged by size: 2716 2612 248 BbsI GAAGACnn'nnnn_ Cuts at: 1254 2378 1254 Size: 1124 4452 BbvCI CC'TCA_GC Cuts at: 2422 2422 Size: 5576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 694 728 694 Size: 34 5542 BciVI GTATCCnnnnn_n' Cuts at: 189 1381 2552 3474 4235 5576 189 Size: 1192 1171 922 761 1341 189 Fragments arranged by size: 1341 1192 1171 922 761 189 BclI T'GATC_A Cuts at: 314 3096 314 Size: 2782 2794 BglI GCCn_nnn'nGGC Cuts at: 870 1020 870 Size: 150 5426 BglII A'GATC_T Cuts at: 2192 3091 5128 2192 Size: 899 2037 2640 Fragments arranged by size: 2640 2037 899 BlpI GC'TnA_GC Cuts at: 2688 2688 Size: 5576 Bme1580I G_kGCm'C Cuts at: 341 350 1016 1355 2339 3201 3294 4350 341 Size: 9 666 339 984 862 93 1056 1567 Fragments arranged by size: 1567 1056 984 862 666 339 93 9 BmrI ACTGGGnnnn_n' Cuts at: 1564 2788 2869 3204 5348 1564 Size: 1224 81 335 2144 1792 Fragments arranged by size: 2144 1792 1224 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1428 1824 1849 3943 1428 Size: 396 25 2094 3061 Fragments arranged by size: 3061 2094 396 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 768 1255 2105 2489 768 Size: 487 850 384 3855 Fragments arranged by size: 3855 850 487 384 Bpu10I CC'TnA_GC Cuts at: 2422 2422 Size: 5576 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1018 1027 2752 3631 4123 4421 1018 Size: 9 1725 879 492 298 2173 Fragments arranged by size: 2173 1725 879 492 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 1500 2340 5280 1500 Size: 840 2940 1796 Fragments arranged by size: 2940 1796 840 BsaAI yAC'GTr Cuts at: 258 836 987 1130 2929 258 Size: 578 151 143 1799 2905 Fragments arranged by size: 2905 1799 578 151 143 BsaBI GATnn'nnATC Cuts at: 6 3090 5127 6 Size: 3084 2037 455 Fragments arranged by size: 3084 2037 455 BsaHI Gr'CG_yC Cuts at: 395 3007 3255 3957 5453 395 Size: 2612 248 702 1496 518 Fragments arranged by size: 2612 1496 702 518 248 BsaWI w'CCGG_w Cuts at: 496 2765 3286 3964 4238 4385 4796 496 Size: 2269 521 678 274 147 411 1276 Fragments arranged by size: 2269 1276 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 911 941 911 Size: 30 5546 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 935 1404 1906 935 Size: 469 502 4605 Fragments arranged by size: 4605 502 469 BseYI C'CCAG_C Cuts at: 116 645 959 1117 3001 3759 4336 4832 116 Size: 529 314 158 1884 758 577 496 860 Fragments arranged by size: 1884 860 758 577 529 496 314 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1752 2274 1752 Size: 522 5054 BsiEI CG_ry'CG Cuts at: 104 862 3118 3164 4372 104 Size: 758 2256 46 1208 1308 Fragments arranged by size: 2256 1308 1208 758 46 BsiHKAI G_wGCw'C Cuts at: 341 567 1355 2590 3368 3558 4350 341 Size: 226 788 1235 778 190 792 1567 Fragments arranged by size: 1567 1235 792 788 778 226 190 BsmBI CGTCTCn'nnnn_ Cuts at: 917 917 Size: 5576 Bsp1286I G_dGCh'C Cuts at: 341 350 567 1016 1355 1842 2212 2339 2590 Size: 9 217 449 339 487 370 127 251 Cuts at: 2590 3201 3294 3368 3558 3620 4350 341 Size: 611 93 74 190 62 730 1567 Fragments arranged by size: 1567 730 611 487 449 370 339 251 217 190 127 93 74 62 9 BspEI T'CCGG_A Cuts at: 496 4796 496 Size: 4300 1276 BspHI T'CATG_A Cuts at: 4752 4752 Size: 5576 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2626 2840 3142 3523 2626 Size: 214 302 381 4679 Fragments arranged by size: 4679 381 302 214 BsrBI CCG'CTC Cuts at: 50 3868 3922 5406 5571 50 Size: 3818 54 1484 165 55 Fragments arranged by size: 3818 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3488 3488 Size: 5576 BsrFI r'CCGG_y Cuts at: 869 894 1051 1344 3574 3755 3964 869 Size: 25 157 293 2230 181 209 2481 Fragments arranged by size: 2481 2230 293 209 181 157 25 BsrGI T'GTAC_A Cuts at: 2303 2303 Size: 5576 BssSI C'ACGA_G Cuts at: 3847 4205 3847 Size: 358 5218 BstAPI GCAn_nnn'nTGC Cuts at: 2334 2953 2334 Size: 619 4957 BstBI TT'CG_AA Cuts at: 3937 3937 Size: 5576 BstEII G'GTnAC_C Cuts at: 144 1641 144 Size: 1497 4079 BstXI CCAn_nnnn'nTGG Cuts at: 1947 2111 1947 Size: 164 5412 BstYI r'GATC_y Cuts at: 1 2192 2601 3068 3091 3426 3672 4684 4770 Size: 2191 409 467 23 335 246 1012 86 Cuts at: 4770 4782 5128 1 Size: 12 346 449 Fragments arranged by size: 2191 1012 467 449 409 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 2145 2145 Size: 5576 Bsu36I CC'TnA_GG Cuts at: 1889 1889 Size: 5576 BtgI C'CryG_G Cuts at: 107 149 1064 1646 2231 107 Size: 42 915 582 585 3452 Fragments arranged by size: 3452 915 585 582 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 697 3526 3707 3870 697 Size: 2829 181 163 2403 Fragments arranged by size: 2829 2403 181 163 BtsI GCAGTG_nn' Cuts at: 1613 2339 2565 2819 1613 Size: 726 226 254 4370 Fragments arranged by size: 4370 726 254 226 DraI TTT'AAA Cuts at: 2575 2575 Size: 5576 DrdI GACnn_nn'nnGTC Cuts at: 3282 4140 3282 Size: 858 4718 EaeI y'GGCC_r Cuts at: 620 1649 1937 3161 3335 3726 3753 5098 620 Size: 1029 288 1224 174 391 27 1345 1098 Fragments arranged by size: 1345 1224 1098 1029 391 288 174 27 EagI C'GGCC_G Cuts at: 3161 3161 Size: 5576 EarI CTCTTCn'nnn_ Cuts at: 209 1194 1467 3599 3809 209 Size: 985 273 2132 210 1976 Fragments arranged by size: 2132 1976 985 273 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1615 3784 4094 4240 5386 1615 Size: 2169 310 146 1146 1805 Fragments arranged by size: 2169 1805 1146 310 146 EcoICRI GAG'CTC Cuts at: 2588 2588 Size: 5576 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 165 768 1255 1317 2105 2275 2465 2489 3401 Size: 603 487 62 788 170 190 24 912 Cuts at: 3401 3833 4580 4848 165 Size: 432 747 268 893 Fragments arranged by size: 912 893 788 747 603 487 432 268 190 170 62 24 EcoO109I rG'GnC_Cy Cuts at: 346 1518 2715 346 Size: 1172 1197 3207 Fragments arranged by size: 3207 1197 1172 EcoRI G'AATT_C Cuts at: 2384 2580 2384 Size: 196 5380 EcoRV GAT'ATC Cuts at: 1157 2058 1157 Size: 901 4675 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1347 2489 3595 1347 Size: 1142 1106 3328 Fragments arranged by size: 3328 1142 1106 FspI TGC'GCA Cuts at: 3357 3357 Size: 5576 HaeII r_GCGC'y Cuts at: 398 1195 3010 3258 4280 5259 398 Size: 797 1815 248 1022 979 715 Fragments arranged by size: 1815 1022 979 797 715 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 475 507 1687 1719 1903 1935 2152 2184 3418 Size: 32 1180 32 184 32 217 32 1234 Cuts at: 3418 3448 3450 3480 475 Size: 30 2 30 2571 Fragments arranged by size: 2571 1234 1180 217 184 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1059 1178 2615 1059 Size: 119 1437 4020 Fragments arranged by size: 4020 1437 119 HpaI GTT'AAC Cuts at: 1059 1059 Size: 5576 KasI G'GCGC_C Cuts at: 394 3006 3254 394 Size: 2612 248 2716 Fragments arranged by size: 2716 2612 248 KpnI G_GTAC'C Cuts at: 2596 2596 Size: 5576 MluI A'CGCG_T Cuts at: 5009 5009 Size: 5576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 301 897 1451 4247 4431 301 Size: 596 554 2796 184 1446 Fragments arranged by size: 2796 1446 596 554 184 MscI TGG'CCA Cuts at: 1939 3337 1939 Size: 1398 4178 MslI CAynn'nnrTG Cuts at: 845 1480 2156 2311 3565 3692 845 Size: 635 676 155 1254 127 2729 Fragments arranged by size: 2729 1254 676 635 155 127 MspA1I CmG'CkG Cuts at: 116 1437 1935 2687 3001 3361 4374 4619 116 Size: 1321 498 752 314 360 1013 245 1073 Fragments arranged by size: 1321 1073 1013 752 498 360 314 245 NaeI GCC'GGC Cuts at: 896 1346 3757 896 Size: 450 2411 2715 Fragments arranged by size: 2715 2411 450 NarI GG'CG_CC Cuts at: 395 3007 3255 395 Size: 2612 248 2716 Fragments arranged by size: 2716 2612 248 NcoI C'CATG_G Cuts at: 107 149 107 Size: 42 5534 NgoMIV G'CCGG_C Cuts at: 894 1344 3755 894 Size: 450 2411 2715 Fragments arranged by size: 2715 2411 450 NspI r_CATG'y Cuts at: 2332 2629 3129 4036 2332 Size: 297 500 907 3872 Fragments arranged by size: 3872 907 500 297 PasI CC'CwG_GG Cuts at: 1566 2221 1566 Size: 655 4921 PciI A'CATG_T Cuts at: 4032 4032 Size: 5576 PflMI CCAn_nnn'nTGG Cuts at: 677 1195 677 Size: 518 5058 PmeI GTTT'AAAC Cuts at: 2575 2575 Size: 5576 PmlI CAC'GTG Cuts at: 987 987 Size: 5576 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 4715 Size: 32 5544 PpuMI rG'GwC_Cy Cuts at: 1518 1518 Size: 5576 PspOMI G'GGCC_C Cuts at: 346 1012 346 Size: 666 4910 PstI C_TGCA'G Cuts at: 2623 3308 2623 Size: 685 4891 PvuI CG_AT'CG Cuts at: 104 862 104 Size: 758 4818 PvuII CAG'CTG Cuts at: 116 1935 3001 3361 116 Size: 1819 1066 360 2331 Fragments arranged by size: 2331 1819 1066 360 RsrII CG'GwC_CG Cuts at: 3771 3771 Size: 5576 SacI G_AGCT'C Cuts at: 2590 2590 Size: 5576 SalI G'TCGA_C Cuts at: 2613 2613 Size: 5576 SapI GCTCTTCn'nnn_ Cuts at: 209 3599 3809 209 Size: 3390 210 1976 Fragments arranged by size: 3390 1976 210 SbfI CC_TGCA'GG Cuts at: 2623 2623 Size: 5576 ScaI AGT'ACT Cuts at: 379 1864 1954 5093 379 Size: 1485 90 3139 862 Fragments arranged by size: 3139 1485 862 90 SfcI C'TryA_G Cuts at: 33 443 2619 3304 4297 4488 33 Size: 410 2176 685 993 191 1121 Fragments arranged by size: 2176 1121 993 685 410 191 SfoI GGC'GCC Cuts at: 396 3008 3256 396 Size: 2612 248 2716 Fragments arranged by size: 2716 2612 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5576 SgrAI Cr'CCGG_yG Cuts at: 1051 1051 Size: 5576 SmaI CCC'GGG Cuts at: 1854 2598 1854 Size: 744 4832 SmlI C'TyrA_G Cuts at: 1033 1042 2731 3646 4138 4400 1033 Size: 9 1689 915 492 262 2209 Fragments arranged by size: 2209 1689 915 492 262 9 SpeI A'CTAG_T Cuts at: 67 67 Size: 5576 SphI G_CATG'C Cuts at: 2332 2629 3129 2332 Size: 297 500 4779 Fragments arranged by size: 4779 500 297 StyI C'CwwG_G Cuts at: 107 149 1631 2710 3064 107 Size: 42 1482 1079 354 2619 Fragments arranged by size: 2619 1482 1079 354 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3159 3292 3751 3960 3159 Size: 133 459 209 4775 Fragments arranged by size: 4775 459 209 133 TatI w'GTAC_w Cuts at: 377 427 1862 1952 2303 5091 377 Size: 50 1435 90 351 2788 862 Fragments arranged by size: 2788 1435 862 351 90 50 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1114 1146 5093 5125 1114 Size: 32 3947 32 1565 Fragments arranged by size: 3947 1565 32 32 Tth111I GACn'n_nGTC Cuts at: 3373 3373 Size: 5576 XbaI T'CTAG_A Cuts at: 2607 2607 Size: 5576 XcmI CCAnnnn_n'nnnnTGG Cuts at: 674 1434 2001 3071 674 Size: 760 567 1070 3179 Fragments arranged by size: 3179 1070 760 567 XmaI C'CCGG_G Cuts at: 1852 2596 1852 Size: 744 4832 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AloI AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BssHII ClaI CspCI DraIII EcoNI FseI FspAI HindIII MfeI NdeI NheI NotI NruI NsiI PacI PfoI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI