Restriction map for
pF1KE2062
(Circular) (Six-base) MAPSORT of: pF1KE2062.seq Check: 2661 from: 1 to: 5576
pF1KE2062 5576 bp
With 182 enzymes: SgfI *
July 8, 2010 13:19 ..
AccI GT'mk_AC
Cuts at: 446 2144 2614 446
Size: 1698 470 3408
Fragments arranged by size:
3408 1698 470
Acc65I G'GTAC_C
Cuts at: 2592 2592
Size: 5576
AclI AA'CG_TT
Cuts at: 2353 5416 2353
Size: 3063 2513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1317 2275 2465 3401 3833 4580 4848 165
Size: 1152 958 190 936 432 747 268 893
Fragments arranged by size:
1152 958 936 893 747 432 268 190
AflIII A'CryG_T
Cuts at: 833 2928 4032 5009 833
Size: 2095 1104 977 1400
Fragments arranged by size:
2095 1400 1104 977
AgeI A'CCGG_T
Cuts at: 3964 3964
Size: 5576
AhdI GACnn_n'nnGTC
Cuts at: 1917 1917
Size: 5576
AlwNI CAG_nnn'CTG
Cuts at: 1195 2881 4448 1195
Size: 1686 1567 2323
Fragments arranged by size:
2323 1686 1567
ApaI G_GGCC'C
Cuts at: 350 1016 350
Size: 666 4910
ApaLI G'TGCA_C
Cuts at: 337 1351 4346 337
Size: 1014 2995 1567
Fragments arranged by size:
2995 1567 1014
ApoI r'AATT_y
Cuts at: 2384 2580 4929 5185 2384
Size: 196 2349 256 2775
Fragments arranged by size:
2775 2349 256 196
AseI AT'TA_AT
Cuts at: 20 20
Size: 5576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5576
AvaI C'yCGr_G
Cuts at: 1852 2596 1852
Size: 744 4832
BamHI G'GATC_C
Cuts at: 2601 2601
Size: 5576
BanI G'GyrC_C
Cuts at: 394 2592 3006 3254 3289 394
Size: 2198 414 248 35 2681
Fragments arranged by size:
2681 2198 414 248 35
BanII G_rGCy'C
Cuts at: 350 1016 1842 2212 2590 3620 350
Size: 666 826 370 378 1030 2306
Fragments arranged by size:
2306 1030 826 666 378 370
BbeI G_GCGC'C
Cuts at: 398 3010 3258 398
Size: 2612 248 2716
Fragments arranged by size:
2716 2612 248
BbsI GAAGACnn'nnnn_
Cuts at: 1254 2378 1254
Size: 1124 4452
BbvCI CC'TCA_GC
Cuts at: 2422 2422
Size: 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 728 694
Size: 34 5542
BciVI GTATCCnnnnn_n'
Cuts at: 189 1381 2552 3474 4235 5576 189
Size: 1192 1171 922 761 1341 189
Fragments arranged by size:
1341 1192 1171 922 761 189
BclI T'GATC_A
Cuts at: 314 3096 314
Size: 2782 2794
BglI GCCn_nnn'nGGC
Cuts at: 870 1020 870
Size: 150 5426
BglII A'GATC_T
Cuts at: 2192 3091 5128 2192
Size: 899 2037 2640
Fragments arranged by size:
2640 2037 899
BlpI GC'TnA_GC
Cuts at: 2688 2688
Size: 5576
Bme1580I G_kGCm'C
Cuts at: 341 350 1016 1355 2339 3201 3294 4350 341
Size: 9 666 339 984 862 93 1056 1567
Fragments arranged by size:
1567 1056 984 862 666 339 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 1564 2788 2869 3204 5348 1564
Size: 1224 81 335 2144 1792
Fragments arranged by size:
2144 1792 1224 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1428 1824 1849 3943 1428
Size: 396 25 2094 3061
Fragments arranged by size:
3061 2094 396 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 768 1255 2105 2489 768
Size: 487 850 384 3855
Fragments arranged by size:
3855 850 487 384
Bpu10I CC'TnA_GC
Cuts at: 2422 2422
Size: 5576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1027 2752 3631 4123 4421 1018
Size: 9 1725 879 492 298 2173
Fragments arranged by size:
2173 1725 879 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 1500 2340 5280 1500
Size: 840 2940 1796
Fragments arranged by size:
2940 1796 840
BsaAI yAC'GTr
Cuts at: 258 836 987 1130 2929 258
Size: 578 151 143 1799 2905
Fragments arranged by size:
2905 1799 578 151 143
BsaBI GATnn'nnATC
Cuts at: 6 3090 5127 6
Size: 3084 2037 455
Fragments arranged by size:
3084 2037 455
BsaHI Gr'CG_yC
Cuts at: 395 3007 3255 3957 5453 395
Size: 2612 248 702 1496 518
Fragments arranged by size:
2612 1496 702 518 248
BsaWI w'CCGG_w
Cuts at: 496 2765 3286 3964 4238 4385 4796 496
Size: 2269 521 678 274 147 411 1276
Fragments arranged by size:
2269 1276 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 911 941 911
Size: 30 5546
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 935 1404 1906 935
Size: 469 502 4605
Fragments arranged by size:
4605 502 469
BseYI C'CCAG_C
Cuts at: 116 645 959 1117 3001 3759 4336 4832 116
Size: 529 314 158 1884 758 577 496 860
Fragments arranged by size:
1884 860 758 577 529 496 314 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1752 2274 1752
Size: 522 5054
BsiEI CG_ry'CG
Cuts at: 104 862 3118 3164 4372 104
Size: 758 2256 46 1208 1308
Fragments arranged by size:
2256 1308 1208 758 46
BsiHKAI G_wGCw'C
Cuts at: 341 567 1355 2590 3368 3558 4350 341
Size: 226 788 1235 778 190 792 1567
Fragments arranged by size:
1567 1235 792 788 778 226 190
BsmBI CGTCTCn'nnnn_
Cuts at: 917 917
Size: 5576
Bsp1286I G_dGCh'C
Cuts at: 341 350 567 1016 1355 1842 2212 2339 2590
Size: 9 217 449 339 487 370 127 251
Cuts at: 2590 3201 3294 3368 3558 3620 4350 341
Size: 611 93 74 190 62 730 1567
Fragments arranged by size:
1567 730 611 487 449 370 339 251
217 190 127 93 74 62 9
BspEI T'CCGG_A
Cuts at: 496 4796 496
Size: 4300 1276
BspHI T'CATG_A
Cuts at: 4752 4752
Size: 5576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2626 2840 3142 3523 2626
Size: 214 302 381 4679
Fragments arranged by size:
4679 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3868 3922 5406 5571 50
Size: 3818 54 1484 165 55
Fragments arranged by size:
3818 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3488 3488
Size: 5576
BsrFI r'CCGG_y
Cuts at: 869 894 1051 1344 3574 3755 3964 869
Size: 25 157 293 2230 181 209 2481
Fragments arranged by size:
2481 2230 293 209 181 157 25
BsrGI T'GTAC_A
Cuts at: 2303 2303
Size: 5576
BssSI C'ACGA_G
Cuts at: 3847 4205 3847
Size: 358 5218
BstAPI GCAn_nnn'nTGC
Cuts at: 2334 2953 2334
Size: 619 4957
BstBI TT'CG_AA
Cuts at: 3937 3937
Size: 5576
BstEII G'GTnAC_C
Cuts at: 144 1641 144
Size: 1497 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 1947 2111 1947
Size: 164 5412
BstYI r'GATC_y
Cuts at: 1 2192 2601 3068 3091 3426 3672 4684 4770
Size: 2191 409 467 23 335 246 1012 86
Cuts at: 4770 4782 5128 1
Size: 12 346 449
Fragments arranged by size:
2191 1012 467 449 409 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 2145 2145
Size: 5576
Bsu36I CC'TnA_GG
Cuts at: 1889 1889
Size: 5576
BtgI C'CryG_G
Cuts at: 107 149 1064 1646 2231 107
Size: 42 915 582 585 3452
Fragments arranged by size:
3452 915 585 582 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 697 3526 3707 3870 697
Size: 2829 181 163 2403
Fragments arranged by size:
2829 2403 181 163
BtsI GCAGTG_nn'
Cuts at: 1613 2339 2565 2819 1613
Size: 726 226 254 4370
Fragments arranged by size:
4370 726 254 226
DraI TTT'AAA
Cuts at: 2575 2575
Size: 5576
DrdI GACnn_nn'nnGTC
Cuts at: 3282 4140 3282
Size: 858 4718
EaeI y'GGCC_r
Cuts at: 620 1649 1937 3161 3335 3726 3753 5098 620
Size: 1029 288 1224 174 391 27 1345 1098
Fragments arranged by size:
1345 1224 1098 1029 391 288 174 27
EagI C'GGCC_G
Cuts at: 3161 3161
Size: 5576
EarI CTCTTCn'nnn_
Cuts at: 209 1194 1467 3599 3809 209
Size: 985 273 2132 210 1976
Fragments arranged by size:
2132 1976 985 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1615 3784 4094 4240 5386 1615
Size: 2169 310 146 1146 1805
Fragments arranged by size:
2169 1805 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2588 2588
Size: 5576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 768 1255 1317 2105 2275 2465 2489 3401
Size: 603 487 62 788 170 190 24 912
Cuts at: 3401 3833 4580 4848 165
Size: 432 747 268 893
Fragments arranged by size:
912 893 788 747 603 487 432 268
190 170 62 24
EcoO109I rG'GnC_Cy
Cuts at: 346 1518 2715 346
Size: 1172 1197 3207
Fragments arranged by size:
3207 1197 1172
EcoRI G'AATT_C
Cuts at: 2384 2580 2384
Size: 196 5380
EcoRV GAT'ATC
Cuts at: 1157 2058 1157
Size: 901 4675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1347 2489 3595 1347
Size: 1142 1106 3328
Fragments arranged by size:
3328 1142 1106
FspI TGC'GCA
Cuts at: 3357 3357
Size: 5576
HaeII r_GCGC'y
Cuts at: 398 1195 3010 3258 4280 5259 398
Size: 797 1815 248 1022 979 715
Fragments arranged by size:
1815 1022 979 797 715 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 1687 1719 1903 1935 2152 2184 3418
Size: 32 1180 32 184 32 217 32 1234
Cuts at: 3418 3448 3450 3480 475
Size: 30 2 30 2571
Fragments arranged by size:
2571 1234 1180 217 184 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1059 1178 2615 1059
Size: 119 1437 4020
Fragments arranged by size:
4020 1437 119
HpaI GTT'AAC
Cuts at: 1059 1059
Size: 5576
KasI G'GCGC_C
Cuts at: 394 3006 3254 394
Size: 2612 248 2716
Fragments arranged by size:
2716 2612 248
KpnI G_GTAC'C
Cuts at: 2596 2596
Size: 5576
MluI A'CGCG_T
Cuts at: 5009 5009
Size: 5576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 301 897 1451 4247 4431 301
Size: 596 554 2796 184 1446
Fragments arranged by size:
2796 1446 596 554 184
MscI TGG'CCA
Cuts at: 1939 3337 1939
Size: 1398 4178
MslI CAynn'nnrTG
Cuts at: 845 1480 2156 2311 3565 3692 845
Size: 635 676 155 1254 127 2729
Fragments arranged by size:
2729 1254 676 635 155 127
MspA1I CmG'CkG
Cuts at: 116 1437 1935 2687 3001 3361 4374 4619 116
Size: 1321 498 752 314 360 1013 245 1073
Fragments arranged by size:
1321 1073 1013 752 498 360 314 245
NaeI GCC'GGC
Cuts at: 896 1346 3757 896
Size: 450 2411 2715
Fragments arranged by size:
2715 2411 450
NarI GG'CG_CC
Cuts at: 395 3007 3255 395
Size: 2612 248 2716
Fragments arranged by size:
2716 2612 248
NcoI C'CATG_G
Cuts at: 107 149 107
Size: 42 5534
NgoMIV G'CCGG_C
Cuts at: 894 1344 3755 894
Size: 450 2411 2715
Fragments arranged by size:
2715 2411 450
NspI r_CATG'y
Cuts at: 2332 2629 3129 4036 2332
Size: 297 500 907 3872
Fragments arranged by size:
3872 907 500 297
PasI CC'CwG_GG
Cuts at: 1566 2221 1566
Size: 655 4921
PciI A'CATG_T
Cuts at: 4032 4032
Size: 5576
PflMI CCAn_nnn'nTGG
Cuts at: 677 1195 677
Size: 518 5058
PmeI GTTT'AAAC
Cuts at: 2575 2575
Size: 5576
PmlI CAC'GTG
Cuts at: 987 987
Size: 5576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 4715
Size: 32 5544
PpuMI rG'GwC_Cy
Cuts at: 1518 1518
Size: 5576
PspOMI G'GGCC_C
Cuts at: 346 1012 346
Size: 666 4910
PstI C_TGCA'G
Cuts at: 2623 3308 2623
Size: 685 4891
PvuI CG_AT'CG
Cuts at: 104 862 104
Size: 758 4818
PvuII CAG'CTG
Cuts at: 116 1935 3001 3361 116
Size: 1819 1066 360 2331
Fragments arranged by size:
2331 1819 1066 360
RsrII CG'GwC_CG
Cuts at: 3771 3771
Size: 5576
SacI G_AGCT'C
Cuts at: 2590 2590
Size: 5576
SalI G'TCGA_C
Cuts at: 2613 2613
Size: 5576
SapI GCTCTTCn'nnn_
Cuts at: 209 3599 3809 209
Size: 3390 210 1976
Fragments arranged by size:
3390 1976 210
SbfI CC_TGCA'GG
Cuts at: 2623 2623
Size: 5576
ScaI AGT'ACT
Cuts at: 379 1864 1954 5093 379
Size: 1485 90 3139 862
Fragments arranged by size:
3139 1485 862 90
SfcI C'TryA_G
Cuts at: 33 443 2619 3304 4297 4488 33
Size: 410 2176 685 993 191 1121
Fragments arranged by size:
2176 1121 993 685 410 191
SfoI GGC'GCC
Cuts at: 396 3008 3256 396
Size: 2612 248 2716
Fragments arranged by size:
2716 2612 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5576
SgrAI Cr'CCGG_yG
Cuts at: 1051 1051
Size: 5576
SmaI CCC'GGG
Cuts at: 1854 2598 1854
Size: 744 4832
SmlI C'TyrA_G
Cuts at: 1033 1042 2731 3646 4138 4400 1033
Size: 9 1689 915 492 262 2209
Fragments arranged by size:
2209 1689 915 492 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5576
SphI G_CATG'C
Cuts at: 2332 2629 3129 2332
Size: 297 500 4779
Fragments arranged by size:
4779 500 297
StyI C'CwwG_G
Cuts at: 107 149 1631 2710 3064 107
Size: 42 1482 1079 354 2619
Fragments arranged by size:
2619 1482 1079 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3159 3292 3751 3960 3159
Size: 133 459 209 4775
Fragments arranged by size:
4775 459 209 133
TatI w'GTAC_w
Cuts at: 377 427 1862 1952 2303 5091 377
Size: 50 1435 90 351 2788 862
Fragments arranged by size:
2788 1435 862 351 90 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1114 1146 5093 5125 1114
Size: 32 3947 32 1565
Fragments arranged by size:
3947 1565 32 32
Tth111I GACn'n_nGTC
Cuts at: 3373 3373
Size: 5576
XbaI T'CTAG_A
Cuts at: 2607 2607
Size: 5576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 674 1434 2001 3071 674
Size: 760 567 1070 3179
Fragments arranged by size:
3179 1070 760 567
XmaI C'CCGG_G
Cuts at: 1852 2596 1852
Size: 744 4832
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BssHII
ClaI CspCI DraIII EcoNI FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI