Restriction map for
pF1KE2067
(Circular) (Six-base) MAPSORT of: pF1KE2067.seq Check: 6203 from: 1 to: 5291
pF1KE2067 5291 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 797 958 1040 1893 1908 797
Size: 161 82 853 15 4180
Fragments arranged by size:
4180 853 161 82 15
AccI GT'mk_AC
Cuts at: 527 1702 2329 527
Size: 1175 627 3489
Fragments arranged by size:
3489 1175 627
Acc65I G'GTAC_C
Cuts at: 2307 2307
Size: 5291
AclI AA'CG_TT
Cuts at: 5131 5131
Size: 5291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1761 3116 3548 4295 4563 1761
Size: 1355 432 747 268 2489
Fragments arranged by size:
2489 1355 747 432 268
AflIII A'CryG_T
Cuts at: 1250 2643 3747 4724 1250
Size: 1393 1104 977 1817
Fragments arranged by size:
1817 1393 1104 977
AgeI A'CCGG_T
Cuts at: 3679 3679
Size: 5291
AleI CACnn'nnGTG
Cuts at: 1292 1292
Size: 5291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1546 1546
Size: 5291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 334
Size: 32 5259
AlwNI CAG_nnn'CTG
Cuts at: 707 1665 2596 4163 707
Size: 958 931 1567 1835
Fragments arranged by size:
1835 1567 958 931
ApaI G_GGCC'C
Cuts at: 366 558 366
Size: 192 5099
ApaLI G'TGCA_C
Cuts at: 4061 4061
Size: 5291
ApoI r'AATT_y
Cuts at: 2230 2295 4644 4900 2230
Size: 65 2349 256 2621
Fragments arranged by size:
2621 2349 256 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 5291
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5291
AvaI C'yCGr_G
Cuts at: 127 193 2311 127
Size: 66 2118 3107
Fragments arranged by size:
3107 2118 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1042 1075 1042
Size: 33 5258
BamHI G'GATC_C
Cuts at: 2316 2316
Size: 5291
BanI G'GyrC_C
Cuts at: 1345 1353 1764 2307 2721 2969 3004 1345
Size: 8 411 543 414 248 35 3632
Fragments arranged by size:
3632 543 414 411 248 35 8
BanII G_rGCy'C
Cuts at: 366 558 1138 1921 1948 2024 2197 2305 3335
Size: 192 580 783 27 76 173 108 1030
Cuts at: 3335 366
Size: 2322
Fragments arranged by size:
2322 1030 783 580 192 173 108 76
27
BbeI G_GCGC'C
Cuts at: 2725 2973 2725
Size: 248 5043
BbsI GAAGACnn'nnnn_
Cuts at: 402 1402 1996 402
Size: 1000 594 3697
Fragments arranged by size:
3697 1000 594
BbvCI CC'TCA_GC
Cuts at: 2092 2092
Size: 5291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 797 831 1162 1196 797
Size: 34 331 34 4892
Fragments arranged by size:
4892 331 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1731 3189 3950 5291 1731
Size: 1458 761 1341 1731
Fragments arranged by size:
1731 1458 1341 761
BclI T'GATC_A
Cuts at: 614 662 2811 614
Size: 48 2149 3094
Fragments arranged by size:
3094 2149 48
BglII A'GATC_T
Cuts at: 1022 2806 4843 1022
Size: 1784 2037 1470
Fragments arranged by size:
2037 1784 1470
BlpI GC'TnA_GC
Cuts at: 2403 2403
Size: 5291
Bme1580I G_kGCm'C
Cuts at: 366 558 1350 1358 1769 2916 3009 4065 366
Size: 192 792 8 411 1147 93 1056 1592
Fragments arranged by size:
1592 1147 1056 792 411 192 93 8
BmgBI CAC'GTC
Cuts at: 771 771
Size: 5291
BmrI ACTGGGnnnn_n'
Cuts at: 709 2503 2584 2919 5063 709
Size: 1794 81 335 2144 937
Fragments arranged by size:
2144 1794 937 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 153 1152 1896 3658 153
Size: 999 744 1762 1786
Fragments arranged by size:
1786 1762 999 744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 1615 1892 1123
Size: 492 277 4522
Fragments arranged by size:
4522 492 277
Bpu10I CC'TnA_GC
Cuts at: 2092 2104 2092
Size: 12 5279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2467 3346 3838 4136 2467
Size: 879 492 298 3622
Fragments arranged by size:
3622 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4995 4995
Size: 5291
BsaAI yAC'GTr
Cuts at: 1253 1490 2644 1253
Size: 237 1154 3900
Fragments arranged by size:
3900 1154 237
BsaBI GATnn'nnATC
Cuts at: 6 1540 2805 4842 6
Size: 1534 1265 2037 455
Fragments arranged by size:
2037 1534 1265 455
BsaHI Gr'CG_yC
Cuts at: 445 2722 2970 3672 5168 445
Size: 2277 248 702 1496 568
Fragments arranged by size:
2277 1496 702 568 248
BsaWI w'CCGG_w
Cuts at: 2480 3001 3679 3953 4100 4511 2480
Size: 521 678 274 147 411 3260
Fragments arranged by size:
3260 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 674 704 1183 1213 334
Size: 30 310 30 479 30 4412
Fragments arranged by size:
4412 479 310 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 339 1021 1351 1473 1771 1819 2080 2083
Size: 23 682 330 122 298 48 261 3
Cuts at: 2083 316
Size: 3524
Fragments arranged by size:
3524 682 330 298 261 122 48 23
3
BseYI C'CCAG_C
Cuts at: 551 557 1185 1658 1920 2033 2716 3474 4051
Size: 6 628 473 262 113 683 758 577
Cuts at: 4051 4547 551
Size: 496 1295
Fragments arranged by size:
1295 758 683 628 577 496 473 262
113 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 775 1162 1880 1933 724
Size: 51 387 718 53 4082
Fragments arranged by size:
4082 718 387 53 51
BsiEI CG_ry'CG
Cuts at: 104 918 2833 2879 4087 104
Size: 814 1915 46 1208 1308
Fragments arranged by size:
1915 1308 1208 814 46
BsiHKAI G_wGCw'C
Cuts at: 1159 2305 3083 3273 4065 1159
Size: 1146 778 190 792 2385
Fragments arranged by size:
2385 1146 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 112 1724 1753 112
Size: 1612 29 3650
Fragments arranged by size:
3650 1612 29
Bsp1286I G_dGCh'C
Cuts at: 366 558 1138 1159 1350 1358 1769 1921 1948
Size: 192 580 21 191 8 411 152 27
Cuts at: 1948 2024 2197 2305 2916 3009 3083 3273 3335
Size: 76 173 108 611 93 74 190 62
Cuts at: 3335 4065 366
Size: 730 1592
Fragments arranged by size:
1592 730 611 580 411 192 191 190
173 152 108 93 76 74 62 27
21 8
BspEI T'CCGG_A
Cuts at: 4511 4511
Size: 5291
BspHI T'CATG_A
Cuts at: 1211 4467 1211
Size: 3256 2035
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 797 935 958 1040 1436 1893 1908 2341
Size: 561 138 23 82 396 457 15 433
Cuts at: 2341 2555 2857 3238 236
Size: 214 302 381 2289
Fragments arranged by size:
2289 561 457 433 396 381 302 214
138 82 23 15
BsrBI CCG'CTC
Cuts at: 50 498 3583 3637 5121 5286 50
Size: 448 3085 54 1484 165 55
Fragments arranged by size:
3085 1484 448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 3203 1676
Size: 1527 3764
BsrFI r'CCGG_y
Cuts at: 798 1118 3289 3470 3679 798
Size: 320 2171 181 209 2410
Fragments arranged by size:
2410 2171 320 209 181
BssSI C'ACGA_G
Cuts at: 3562 3920 3562
Size: 358 4933
BstAPI GCAn_nnn'nTGC
Cuts at: 707 1894 2668 707
Size: 1187 774 3330
Fragments arranged by size:
3330 1187 774
BstBI TT'CG_AA
Cuts at: 3652 3652
Size: 5291
BstEII G'GTnAC_C
Cuts at: 2166 2166
Size: 5291
BstYI r'GATC_y
Cuts at: 1 956 1022 2316 2783 2806 3141 3387 4399
Size: 955 66 1294 467 23 335 246 1012
Cuts at: 4399 4485 4497 4843 1
Size: 86 12 346 449
Fragments arranged by size:
1294 1012 955 467 449 346 335 246
86 66 23 12
Bsu36I CC'TnA_GG
Cuts at: 623 623
Size: 5291
BtgI C'CryG_G
Cuts at: 975 1313 975
Size: 338 4953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3241 3422 3585 3241
Size: 181 163 4947
Fragments arranged by size:
4947 181 163
BtsI GCAGTG_nn'
Cuts at: 528 645 1150 1530 2534 528
Size: 117 505 380 1004 3285
Fragments arranged by size:
3285 1004 505 380 117
DraI TTT'AAA
Cuts at: 2290 2290
Size: 5291
DrdI GACnn_nn'nnGTC
Cuts at: 574 2997 3855 574
Size: 2423 858 2010
Fragments arranged by size:
2423 2010 858
EaeI y'GGCC_r
Cuts at: 389 485 746 800 818 1442 2876 3050 3441
Size: 96 261 54 18 624 1434 174 391
Cuts at: 3441 3468 4813 389
Size: 27 1345 867
Fragments arranged by size:
1434 1345 867 624 391 261 174 96
54 27 18
EagI C'GGCC_G
Cuts at: 2876 2876
Size: 5291
EarI CTCTTCn'nnn_
Cuts at: 110 1171 1681 1865 3314 3524 110
Size: 1061 510 184 1449 210 1877
Fragments arranged by size:
1877 1449 1061 510 210 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 238 3499 3809 3955 5101 238
Size: 3261 310 146 1146 428
Fragments arranged by size:
3261 1146 428 310 146
EcoICRI GAG'CTC
Cuts at: 2303 2303
Size: 5291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 1615 1761 1892 3116 3548 4295 4563 1123
Size: 492 146 131 1224 432 747 268 1851
Fragments arranged by size:
1851 1224 747 492 432 268 146 131
EcoNI CCTnn'n_nnAGG
Cuts at: 232 294 319 664 1105 232
Size: 62 25 345 441 4418
Fragments arranged by size:
4418 441 345 62 25
EcoO109I rG'GnC_Cy
Cuts at: 363 831 1206 1648 2430 363
Size: 468 375 442 782 3224
Fragments arranged by size:
3224 782 468 442 375
EcoRI G'AATT_C
Cuts at: 2295 2295
Size: 5291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3310 3310
Size: 5291
FspI TGC'GCA
Cuts at: 3072 3072
Size: 5291
HaeII r_GCGC'y
Cuts at: 2725 2973 3995 4974 2725
Size: 248 1022 979 3042
Fragments arranged by size:
3042 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1624 1656 3133 3163 3165 3195 163
Size: 32 1429 32 1477 30 2 30 2259
Fragments arranged by size:
2259 1477 1429 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2055 2330 2055
Size: 275 5016
KasI G'GCGC_C
Cuts at: 2721 2969 2721
Size: 248 5043
KpnI G_GTAC'C
Cuts at: 2311 2311
Size: 5291
MluI A'CGCG_T
Cuts at: 4724 4724
Size: 5291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 1781 3962 4146 377
Size: 1404 2181 184 1522
Fragments arranged by size:
2181 1522 1404 184
MscI TGG'CCA
Cuts at: 391 487 3052 391
Size: 96 2565 2630
Fragments arranged by size:
2630 2565 96
MslI CAynn'nnrTG
Cuts at: 1216 1292 1492 3280 3407 1216
Size: 76 200 1788 127 3100
Fragments arranged by size:
3100 1788 200 127 76
MspA1I CmG'CkG
Cuts at: 469 561 707 811 1403 1645 1716 2174 2402
Size: 92 146 104 592 242 71 458 228
Cuts at: 2402 2716 3076 4089 4334 469
Size: 314 360 1013 245 1426
Fragments arranged by size:
1426 1013 592 458 360 314 245 242
228 146 104 92 71
NaeI GCC'GGC
Cuts at: 3472 3472
Size: 5291
NarI GG'CG_CC
Cuts at: 2722 2970 2722
Size: 248 5043
NcoI C'CATG_G
Cuts at: 975 975
Size: 5291
NgoMIV G'CCGG_C
Cuts at: 3470 3470
Size: 5291
NspI r_CATG'y
Cuts at: 204 1046 1375 1533 2344 2844 3751 204
Size: 842 329 158 811 500 907 1744
Fragments arranged by size:
1744 907 842 811 500 329 158
PciI A'CATG_T
Cuts at: 3747 3747
Size: 5291
PflMI CCAn_nnn'nTGG
Cuts at: 359 827 981 1131 1665 1800 359
Size: 468 154 150 534 135 3850
Fragments arranged by size:
3850 534 468 154 150 135
PfoI T'CCnGG_A
Cuts at: 320 536 1100 320
Size: 216 564 4511
Fragments arranged by size:
4511 564 216
PmeI GTTT'AAAC
Cuts at: 2290 2290
Size: 5291
PmlI CAC'GTG
Cuts at: 1490 1490
Size: 5291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 334 366 4430 4462 334
Size: 32 4064 32 1163
Fragments arranged by size:
4064 1163 32 32
PspOMI G'GGCC_C
Cuts at: 362 554 362
Size: 192 5099
PstI C_TGCA'G
Cuts at: 284 932 1037 2338 3023 284
Size: 648 105 1301 685 2552
Fragments arranged by size:
2552 1301 685 648 105
PvuI CG_AT'CG
Cuts at: 104 918 104
Size: 814 4477
PvuII CAG'CTG
Cuts at: 469 561 707 1716 2716 3076 469
Size: 92 146 1009 1000 360 2684
Fragments arranged by size:
2684 1009 1000 360 146 92
RsrII CG'GwC_CG
Cuts at: 3486 3486
Size: 5291
SacI G_AGCT'C
Cuts at: 2305 2305
Size: 5291
SalI G'TCGA_C
Cuts at: 2328 2328
Size: 5291
SapI GCTCTTCn'nnn_
Cuts at: 1681 1865 3314 3524 1681
Size: 184 1449 210 3448
Fragments arranged by size:
3448 1449 210 184
SbfI CC_TGCA'GG
Cuts at: 1037 2338 1037
Size: 1301 3990
ScaI AGT'ACT
Cuts at: 1592 4808 1592
Size: 3216 2075
SfcI C'TryA_G
Cuts at: 33 280 928 1033 1236 1703 2334 3019 4012
Size: 247 648 105 203 467 631 685 993
Cuts at: 4012 4203 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 648 631 467 247 203
191 105
SfoI GGC'GCC
Cuts at: 2723 2971 2723
Size: 248 5043
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5291
SmaI CCC'GGG
Cuts at: 2313 2313
Size: 5291
SmlI C'TyrA_G
Cuts at: 2446 3361 3853 4115 2446
Size: 915 492 262 3622
Fragments arranged by size:
3622 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5291
SphI G_CATG'C
Cuts at: 1375 1533 2344 2844 1375
Size: 158 811 500 3822
Fragments arranged by size:
3822 811 500 158
StuI AGG'CCT
Cuts at: 314 2102 314
Size: 1788 3503
StyI C'CwwG_G
Cuts at: 939 975 1724 2425 2779 939
Size: 36 749 701 354 3451
Fragments arranged by size:
3451 749 701 354 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 982 2154 2874 3007 3466 3675 982
Size: 1172 720 133 459 209 2598
Fragments arranged by size:
2598 1172 720 459 209 133
TatI w'GTAC_w
Cuts at: 197 1590 1808 4806 197
Size: 1393 218 2998 682
Fragments arranged by size:
2998 1393 682 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 674 706 4808 4840 674
Size: 32 4102 32 1125
Fragments arranged by size:
4102 1125 32 32
Tth111I GACn'n_nGTC
Cuts at: 3088 3088
Size: 5291
XbaI T'CTAG_A
Cuts at: 2322 2322
Size: 5291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2786 2786
Size: 5291
XmaI C'CCGG_G
Cuts at: 2311 2311
Size: 5291
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BfrBI BglI
BmtI BsiWI BsmI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI