Restriction map for
pF1KE2078
(Circular) (Six-base) MAPSORT of: pF1KE2078.seq Check: 6691 from: 1 to: 5357
pF1KE2078 5357 bp
With 182 enzymes: SgfI *
May 14, 2013 10:45 ..
AccI GT'mk_AC
Cuts at: 2395 2395
Size: 5357
Acc65I G'GTAC_C
Cuts at: 2373 2373
Size: 5357
AclI AA'CG_TT
Cuts at: 5197 5197
Size: 5357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 1100 1947 3182 3614 4361 4629 737
Size: 363 847 1235 432 747 268 1465
Fragments arranged by size:
1465 1235 847 747 432 363 268
AflIII A'CryG_T
Cuts at: 1849 2349 2709 3813 4790 1849
Size: 500 360 1104 977 2416
Fragments arranged by size:
2416 1104 977 500 360
AgeI A'CCGG_T
Cuts at: 3745 3745
Size: 5357
AleI CACnn'nnGTG
Cuts at: 248 1788 1964 248
Size: 1540 176 3641
Fragments arranged by size:
3641 1540 176
AlwNI CAG_nnn'CTG
Cuts at: 1744 1942 2662 4229 1744
Size: 198 720 1567 2872
Fragments arranged by size:
2872 1567 720 198
ApaLI G'TGCA_C
Cuts at: 1302 4127 1302
Size: 2825 2532
ApoI r'AATT_y
Cuts at: 361 1017 1323 1399 2361 4710 4966 361
Size: 656 306 76 962 2349 256 752
Fragments arranged by size:
2349 962 752 656 306 256 76
AseI AT'TA_AT
Cuts at: 20 1796 20
Size: 1776 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5357
AvaI C'yCGr_G
Cuts at: 2318 2377 2318
Size: 59 5298
BamHI G'GATC_C
Cuts at: 1276 1993 2382 1276
Size: 717 389 4251
Fragments arranged by size:
4251 717 389
BanI G'GyrC_C
Cuts at: 2373 2787 3035 3070 2373
Size: 414 248 35 4660
Fragments arranged by size:
4660 414 248 35
BanII G_rGCy'C
Cuts at: 2105 2371 3401 2105
Size: 266 1030 4061
Fragments arranged by size:
4061 1030 266
BbeI G_GCGC'C
Cuts at: 2791 3039 2791
Size: 248 5109
BbsI GAAGACnn'nnnn_
Cuts at: 281 318 1050 281
Size: 37 732 4588
Fragments arranged by size:
4588 732 37
BciVI GTATCCnnnnn_n'
Cuts at: 3255 4016 5357 3255
Size: 761 1341 3255
Fragments arranged by size:
3255 1341 761
BclI T'GATC_A
Cuts at: 792 2171 2877 792
Size: 1379 706 3272
Fragments arranged by size:
3272 1379 706
BglII A'GATC_T
Cuts at: 2872 4909 2872
Size: 2037 3320
BlpI GC'TnA_GC
Cuts at: 2469 2469
Size: 5357
Bme1580I G_kGCm'C
Cuts at: 1306 2982 3075 4131 1306
Size: 1676 93 1056 2532
Fragments arranged by size:
2532 1676 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2569 2650 2985 5129 2569
Size: 81 335 2144 2797
Fragments arranged by size:
2797 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2297 3724 2297
Size: 1427 3930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 5357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 1459 2533 3412 3904 4202 683
Size: 776 1074 879 492 298 1838
Fragments arranged by size:
1838 1074 879 776 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1220 2271 5061 1220
Size: 1051 2790 1516
Fragments arranged by size:
2790 1516 1051
BsaAI yAC'GTr
Cuts at: 1850 2710 1850
Size: 860 4497
BsaBI GATnn'nnATC
Cuts at: 6 1568 2871 4908 6
Size: 1562 1303 2037 455
Fragments arranged by size:
2037 1562 1303 455
BsaHI Gr'CG_yC
Cuts at: 2788 3036 3738 5234 2788
Size: 248 702 1496 2911
Fragments arranged by size:
2911 1496 702 248
BsaWI w'CCGG_w
Cuts at: 895 2546 3067 3745 4019 4166 4577 895
Size: 1651 521 678 274 147 411 1675
Fragments arranged by size:
1675 1651 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1834 1864 1834
Size: 30 5327
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 1855 2231 2286 2289 127
Size: 1728 376 55 3 3195
Fragments arranged by size:
3195 1728 376 55 3
BseYI C'CCAG_C
Cuts at: 1737 2303 2782 3540 4117 4613 1737
Size: 566 479 758 577 496 2481
Fragments arranged by size:
2481 758 577 566 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 1635 1829 1966 2061 647
Size: 988 194 137 95 3943
Fragments arranged by size:
3943 988 194 137 95
BsiEI CG_ry'CG
Cuts at: 104 2899 2945 4153 104
Size: 2795 46 1208 1308
Fragments arranged by size:
2795 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1113 1306 2105 2371 3149 3339 4131 1113
Size: 193 799 266 778 190 792 2339
Fragments arranged by size:
2339 799 792 778 266 193 190
BsmI GAATG_Cn'
Cuts at: 1558 1654 1558
Size: 96 5261
BsmBI CGTCTCn'nnnn_
Cuts at: 2340 2340
Size: 5357
Bsp1286I G_dGCh'C
Cuts at: 1113 1306 2105 2371 2982 3075 3149 3339 3401
Size: 193 799 266 611 93 74 190 62
Cuts at: 3401 4131 1113
Size: 730 2339
Fragments arranged by size:
2339 799 730 611 266 193 190 93
74 62
BspEI T'CCGG_A
Cuts at: 895 4577 895
Size: 3682 1675
BspHI T'CATG_A
Cuts at: 576 1032 4533 576
Size: 456 3501 1400
Fragments arranged by size:
3501 1400 456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1801 2407 2621 2923 3304 1801
Size: 606 214 302 381 3854
Fragments arranged by size:
3854 606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3649 3703 5187 5352 50
Size: 3599 54 1484 165 55
Fragments arranged by size:
3599 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1810 2114 3269 1810
Size: 304 1155 3898
Fragments arranged by size:
3898 1155 304
BsrFI r'CCGG_y
Cuts at: 3355 3536 3745 3355
Size: 181 209 4967
Fragments arranged by size:
4967 209 181
BsrGI T'GTAC_A
Cuts at: 1639 1639
Size: 5357
BssSI C'ACGA_G
Cuts at: 497 821 3628 3986 497
Size: 324 2807 358 1868
Fragments arranged by size:
2807 1868 358 324
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 2734 1651
Size: 1083 4274
BstBI TT'CG_AA
Cuts at: 3718 3718
Size: 5357
BstXI CCAn_nnnn'nTGG
Cuts at: 307 499 670 307
Size: 192 171 4994
Fragments arranged by size:
4994 192 171
BstYI r'GATC_y
Cuts at: 1 1276 1993 2382 2849 2872 3207 3453 4465
Size: 1275 717 389 467 23 335 246 1012
Cuts at: 4465 4551 4563 4909 1
Size: 86 12 346 449
Fragments arranged by size:
1275 1012 717 467 449 389 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 614 614
Size: 5357
BtgI C'CryG_G
Cuts at: 107 306 453 107
Size: 199 147 5011
Fragments arranged by size:
5011 199 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3307 3488 3651 3307
Size: 181 163 5013
Fragments arranged by size:
5013 181 163
BtsI GCAGTG_nn'
Cuts at: 1954 2211 2600 1954
Size: 257 389 4711
Fragments arranged by size:
4711 389 257
DraI TTT'AAA
Cuts at: 182 366 807 2267 2356 182
Size: 184 441 1460 89 3183
Fragments arranged by size:
3183 1460 441 184 89
DraIII CAC_nnn'GTG
Cuts at: 1651 1651
Size: 5357
DrdI GACnn_nn'nnGTC
Cuts at: 3063 3921 3063
Size: 858 4499
EaeI y'GGCC_r
Cuts at: 1473 2942 3116 3507 3534 4879 1473
Size: 1469 174 391 27 1345 1951
Fragments arranged by size:
1951 1469 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2942 2942
Size: 5357
EarI CTCTTCn'nnn_
Cuts at: 328 1441 3380 3590 328
Size: 1113 1939 210 2095
Fragments arranged by size:
2095 1939 1113 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3565 3875 4021 5167 3565
Size: 310 146 1146 3755
Fragments arranged by size:
3755 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2103 2369 2103
Size: 266 5091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 737 1100 1148 1947 3182 3614 4361 4629 737
Size: 363 48 799 1235 432 747 268 1465
Fragments arranged by size:
1465 1235 799 747 432 363 268 48
EcoO109I rG'GnC_Cy
Cuts at: 960 1630 2496 960
Size: 670 866 3821
Fragments arranged by size:
3821 866 670
EcoRI G'AATT_C
Cuts at: 1017 2361 1017
Size: 1344 4013
EcoRV GAT'ATC
Cuts at: 845 845
Size: 5357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1194 3376 1194
Size: 2182 3175
FspI TGC'GCA
Cuts at: 3138 3138
Size: 5357
HaeII r_GCGC'y
Cuts at: 2791 3039 4061 5040 2791
Size: 248 1022 979 3108
Fragments arranged by size:
3108 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 834 866 2118 2150 3199 3229 3231
Size: 32 655 32 1252 32 1049 30 2
Cuts at: 3231 3261 147
Size: 30 2243
Fragments arranged by size:
2243 1252 1049 655 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2396 2396
Size: 5357
KasI G'GCGC_C
Cuts at: 2787 3035 2787
Size: 248 5109
KpnI G_GTAC'C
Cuts at: 2377 2377
Size: 5357
MluI A'CGCG_T
Cuts at: 2349 4790 2349
Size: 2441 2916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1086 1242 4028 4212 1086
Size: 156 2786 184 2231
Fragments arranged by size:
2786 2231 184 156
MscI TGG'CCA
Cuts at: 1475 3118 1475
Size: 1643 3714
MslI CAynn'nnrTG
Cuts at: 248 305 428 581 1788 1964 2179 3346 3473
Size: 57 123 153 1207 176 215 1167 127
Cuts at: 3473 248
Size: 2132
Fragments arranged by size:
2132 1207 1167 215 176 153 127 123
57
MspA1I CmG'CkG
Cuts at: 757 2313 2468 2782 3142 4155 4400 757
Size: 1556 155 314 360 1013 245 1714
Fragments arranged by size:
1714 1556 1013 360 314 245 155
NaeI GCC'GGC
Cuts at: 3538 3538
Size: 5357
NarI GG'CG_CC
Cuts at: 2788 3036 2788
Size: 248 5109
NcoI C'CATG_G
Cuts at: 107 306 453 107
Size: 199 147 5011
Fragments arranged by size:
5011 199 147
NgoMIV G'CCGG_C
Cuts at: 3536 3536
Size: 5357
NspI r_CATG'y
Cuts at: 712 2410 2910 3817 712
Size: 1698 500 907 2252
Fragments arranged by size:
2252 1698 907 500
PciI A'CATG_T
Cuts at: 3813 3813
Size: 5357
PflMI CCAn_nnn'nTGG
Cuts at: 991 991
Size: 5357
PmeI GTTT'AAAC
Cuts at: 2356 2356
Size: 5357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 5325
PpuMI rG'GwC_Cy
Cuts at: 960 1630 960
Size: 670 4687
PstI C_TGCA'G
Cuts at: 2404 3089 2404
Size: 685 4672
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5357
PvuII CAG'CTG
Cuts at: 757 2782 3142 757
Size: 2025 360 2972
Fragments arranged by size:
2972 2025 360
RsrII CG'GwC_CG
Cuts at: 3552 3552
Size: 5357
SacI G_AGCT'C
Cuts at: 2105 2371 2105
Size: 266 5091
SalI G'TCGA_C
Cuts at: 2394 2394
Size: 5357
SapI GCTCTTCn'nnn_
Cuts at: 3380 3590 3380
Size: 210 5147
SbfI CC_TGCA'GG
Cuts at: 2404 2404
Size: 5357
ScaI AGT'ACT
Cuts at: 389 4874 389
Size: 4485 872
SfcI C'TryA_G
Cuts at: 33 1410 1530 2400 3085 4078 4269 33
Size: 1377 120 870 685 993 191 1121
Fragments arranged by size:
1377 1121 993 870 685 191 120
SfoI GGC'GCC
Cuts at: 2789 3037 2789
Size: 248 5109
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5357
SmaI CCC'GGG
Cuts at: 2379 2379
Size: 5357
SmlI C'TyrA_G
Cuts at: 698 1438 2512 3427 3919 4181 698
Size: 740 1074 915 492 262 1874
Fragments arranged by size:
1874 1074 915 740 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5357
SphI G_CATG'C
Cuts at: 2410 2910 2410
Size: 500 4857
SspI AAT'ATT
Cuts at: 811 990 811
Size: 179 5178
StyI C'CwwG_G
Cuts at: 107 306 453 2491 2845 107
Size: 199 147 2038 354 2619
Fragments arranged by size:
2619 2038 354 199 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 560 1082 2940 3073 3532 3741 560
Size: 522 1858 133 459 209 2176
Fragments arranged by size:
2176 1858 522 459 209 133
TatI w'GTAC_w
Cuts at: 387 1639 1933 4872 387
Size: 1252 294 2939 872
Fragments arranged by size:
2939 1252 872 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1834 1866 4874 4906 1834
Size: 32 3008 32 2285
Fragments arranged by size:
3008 2285 32 32
Tth111I GACn'n_nGTC
Cuts at: 3154 3154
Size: 5357
XbaI T'CTAG_A
Cuts at: 291 2388 291
Size: 2097 3260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 499 1483 2852 499
Size: 984 1369 3004
Fragments arranged by size:
3004 1369 984
XmaI C'CCGG_G
Cuts at: 2377 2377
Size: 5357
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BssHII BstEII BstZ17I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI