Restriction map for
pF1KE2119
(Circular) (Six-base) MAPSORT of: pF1KE2119.seq Check: 4857 from: 1 to: 5849
pF1KE2119 5849 bp
With 182 enzymes: SgfI *
September 29, 2011 13:09 ..
AccI GT'mk_AC
Cuts at: 362 2887 362
Size: 2525 3324
Acc65I G'GTAC_C
Cuts at: 2341 2865 2341
Size: 524 5325
AclI AA'CG_TT
Cuts at: 725 1038 5689 725
Size: 313 4651 885
Fragments arranged by size:
4651 885 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1000 1529 2819 3674 4106 4853 5121 433
Size: 567 529 1290 855 432 747 268 1161
Fragments arranged by size:
1290 1161 855 747 567 529 432 268
AfeI AGC'GCT
Cuts at: 2587 2680 2587
Size: 93 5756
AflIII A'CryG_T
Cuts at: 2402 3201 4305 5282 2402
Size: 799 1104 977 2969
Fragments arranged by size:
2969 1104 977 799
AgeI A'CCGG_T
Cuts at: 4237 4237
Size: 5849
AhdI GACnn_n'nnGTC
Cuts at: 2406 2406
Size: 5849
AleI CACnn'nnGTG
Cuts at: 2027 2027
Size: 5849
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1281 1313 1281
Size: 32 5817
AlwNI CAG_nnn'CTG
Cuts at: 1117 2739 3154 4721 1117
Size: 1622 415 1567 2245
Fragments arranged by size:
2245 1622 1567 415
ApaI G_GGCC'C
Cuts at: 206 212 206
Size: 6 5843
ApaLI G'TGCA_C
Cuts at: 1043 4619 1043
Size: 3576 2273
ApoI r'AATT_y
Cuts at: 849 1502 1517 2037 2483 2853 5202 5458 849
Size: 653 15 520 446 370 2349 256 1240
Fragments arranged by size:
2349 1240 653 520 446 370 256 15
AseI AT'TA_AT
Cuts at: 20 1085 20
Size: 1065 4784
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5849
AvaI C'yCGr_G
Cuts at: 205 2869 205
Size: 2664 3185
BamHI G'GATC_C
Cuts at: 2874 2874
Size: 5849
BanI G'GyrC_C
Cuts at: 2192 2341 2865 3279 3527 3562 2192
Size: 149 524 414 248 35 4479
Fragments arranged by size:
4479 524 414 248 149 35
BanII G_rGCy'C
Cuts at: 206 212 576 797 1175 1750 2863 3893 206
Size: 6 364 221 378 575 1113 1030 2162
Fragments arranged by size:
2162 1113 1030 575 378 364 221 6
BbeI G_GCGC'C
Cuts at: 3283 3531 3283
Size: 248 5601
BbsI GAAGACnn'nnnn_
Cuts at: 533 2474 533
Size: 1941 3908
BbvCI CC'TCA_GC
Cuts at: 2253 2253
Size: 5849
BciVI GTATCCnnnnn_n'
Cuts at: 2516 3747 4508 5849 2516
Size: 1231 761 1341 2516
Fragments arranged by size:
2516 1341 1231 761
BclI T'GATC_A
Cuts at: 588 2759 3369 588
Size: 2171 610 3068
Fragments arranged by size:
3068 2171 610
BglI GCCn_nnn'nGGC
Cuts at: 175 2441 175
Size: 2266 3583
BglII A'GATC_T
Cuts at: 3364 5401 3364
Size: 2037 3812
BlpI GC'TnA_GC
Cuts at: 1099 2961 1099
Size: 1862 3987
Bme1580I G_kGCm'C
Cuts at: 206 212 786 1047 3474 3567 4623 206
Size: 6 574 261 2427 93 1056 1432
Fragments arranged by size:
2427 1432 1056 574 261 93 6
BmgBI CAC'GTC
Cuts at: 336 336
Size: 5849
BmrI ACTGGGnnnn_n'
Cuts at: 131 746 1882 3061 3142 3477 5621 131
Size: 615 1136 1179 81 335 2144 359
Fragments arranged by size:
2144 1179 1136 615 359 335 81
BmtI G_CTAG'C
Cuts at: 2251 2777 2251
Size: 526 5323
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 134 4216 134
Size: 4082 1767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 560
Size: 5849
Bpu10I CC'TnA_GC
Cuts at: 277 2253 2437 277
Size: 1976 184 3689
Fragments arranged by size:
3689 1976 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2628 3025 3904 4396 4694 2628
Size: 397 879 492 298 3783
Fragments arranged by size:
3783 879 492 397 298
BsaI GGTCTCn'nnnn_
Cuts at: 5553 5553
Size: 5849
BsaAI yAC'GTr
Cuts at: 3202 3202
Size: 5849
BsaBI GATnn'nnATC
Cuts at: 6 1711 3363 5400 6
Size: 1705 1652 2037 455
Fragments arranged by size:
2037 1705 1652 455
BsaHI Gr'CG_yC
Cuts at: 1280 2059 3280 3528 4230 5726 1280
Size: 779 1221 248 702 1496 1403
Fragments arranged by size:
1496 1403 1221 779 702 248
BsaWI w'CCGG_w
Cuts at: 120 2324 3038 3559 4237 4511 4658 5069 120
Size: 2204 714 521 678 274 147 411 900
Fragments arranged by size:
2204 900 714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1610 1640 2035 2065 1610
Size: 30 395 30 5394
Fragments arranged by size:
5394 395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1740 1740
Size: 5849
BseYI C'CCAG_C
Cuts at: 3274 4032 4609 5105 3274
Size: 758 577 496 4018
Fragments arranged by size:
4018 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 1703 1357
Size: 346 5503
BsiEI CG_ry'CG
Cuts at: 104 133 179 245 3391 3437 4645 104
Size: 29 46 66 3146 46 1208 1308
Fragments arranged by size:
3146 1308 1208 66 46 46 29
BsiHKAI G_wGCw'C
Cuts at: 576 1047 2150 2863 3641 3831 4623 576
Size: 471 1103 713 778 190 792 1802
Fragments arranged by size:
1802 1103 792 778 713 471 190
BsmI GAATG_Cn'
Cuts at: 2496 2668 2496
Size: 172 5677
BsmBI CGTCTCn'nnnn_
Cuts at: 2050 2050
Size: 5849
Bsp1286I G_dGCh'C
Cuts at: 206 212 576 786 797 1047 1175 1750 2150
Size: 6 364 210 11 250 128 575 400
Cuts at: 2150 2863 3474 3567 3641 3831 3893 4623 206
Size: 713 611 93 74 190 62 730 1432
Fragments arranged by size:
1432 730 713 611 575 400 364 250
210 190 128 93 74 62 11 6
BspEI T'CCGG_A
Cuts at: 2324 5069 2324
Size: 2745 3104
BspHI T'CATG_A
Cuts at: 1325 1376 2535 5025 1325
Size: 51 1159 2490 2149
Fragments arranged by size:
2490 2149 1159 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1478 2126 2899 3113 3415 3796 1478
Size: 648 773 214 302 381 3531
Fragments arranged by size:
3531 773 648 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4141 4195 5679 5844 50
Size: 4091 54 1484 165 55
Fragments arranged by size:
4091 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1202 1226 3761 1202
Size: 24 2535 3290
Fragments arranged by size:
3290 2535 24
BsrFI r'CCGG_y
Cuts at: 288 3847 4028 4237 288
Size: 3559 181 209 1900
Fragments arranged by size:
3559 1900 209 181
BssSI C'ACGA_G
Cuts at: 1273 2179 4120 4478 1273
Size: 906 1941 358 2644
Fragments arranged by size:
2644 1941 906 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1002 1016 2739 3226 1002
Size: 14 1723 487 3625
Fragments arranged by size:
3625 1723 487 14
BstBI TT'CG_AA
Cuts at: 1583 4210 1583
Size: 2627 3222
BstXI CCAn_nnnn'nTGG
Cuts at: 2519 2519
Size: 5849
BstYI r'GATC_y
Cuts at: 1 407 1257 2874 3341 3364 3699 3945 4957
Size: 406 850 1617 467 23 335 246 1012
Cuts at: 4957 5043 5055 5401 1
Size: 86 12 346 449
Fragments arranged by size:
1617 1012 850 467 449 406 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2270 2363 2371 2707 2270
Size: 93 8 336 5412
Fragments arranged by size:
5412 336 93 8
BtgI C'CryG_G
Cuts at: 152 1754 2094 152
Size: 1602 340 3907
Fragments arranged by size:
3907 1602 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3799 3980 4143 3799
Size: 181 163 5505
Fragments arranged by size:
5505 181 163
BtsI GCAGTG_nn'
Cuts at: 1012 3092 1012
Size: 2080 3769
DraI TTT'AAA
Cuts at: 678 2205 2848 678
Size: 1527 643 3679
Fragments arranged by size:
3679 1527 643
DraIII CAC_nnn'GTG
Cuts at: 956 956
Size: 5849
DrdI GACnn_nn'nnGTC
Cuts at: 3555 4413 3555
Size: 858 4991
EaeI y'GGCC_r
Cuts at: 130 176 464 1691 3434 3608 3999 4026 5371
Size: 46 288 1227 1743 174 391 27 1345
Cuts at: 5371 130
Size: 608
Fragments arranged by size:
1743 1345 1227 608 391 288 174 46
27
EagI C'GGCC_G
Cuts at: 130 176 3434 130
Size: 46 3258 2545
Fragments arranged by size:
3258 2545 46
EarI CTCTTCn'nnn_
Cuts at: 835 1351 1920 3872 4082 835
Size: 516 569 1952 210 2602
Fragments arranged by size:
2602 1952 569 516 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 504 723 4057 4367 4513 5659 504
Size: 219 3334 310 146 1146 694
Fragments arranged by size:
3334 1146 694 310 219 146
EcoICRI GAG'CTC
Cuts at: 574 2861 574
Size: 2287 3562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 560 1000 1529 2819 3674 4106 4853 5121
Size: 127 440 529 1290 855 432 747 268
Cuts at: 5121 433
Size: 1161
Fragments arranged by size:
1290 1161 855 747 529 440 432 268
127
EcoO109I rG'GnC_Cy
Cuts at: 2367 2988 2367
Size: 621 5228
EcoRI G'AATT_C
Cuts at: 2853 2853
Size: 5849
EcoRV GAT'ATC
Cuts at: 474 1785 474
Size: 1311 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 505 3868 505
Size: 3363 2486
FspI TGC'GCA
Cuts at: 3630 3630
Size: 5849
HaeII r_GCGC'y
Cuts at: 116 2589 2682 3283 3531 4553 5532 116
Size: 2473 93 601 248 1022 979 433
Fragments arranged by size:
2473 1022 979 601 433 248 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 829 861 1242 1274 1379 1411 2392
Size: 32 379 32 381 32 105 32 981
Cuts at: 2392 2424 3691 3721 3723 3753 418
Size: 32 1267 30 2 30 2514
Fragments arranged by size:
2514 1267 981 381 379 105 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 363 2888 363
Size: 2525 3324
HindIII A'AGCT_T
Cuts at: 973 973
Size: 5849
KasI G'GCGC_C
Cuts at: 3279 3527 3279
Size: 248 5601
KpnI G_GTAC'C
Cuts at: 2345 2869 2345
Size: 524 5325
MfeI C'AATT_G
Cuts at: 690 690
Size: 5849
MluI A'CGCG_T
Cuts at: 5282 5282
Size: 5849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1990 4520 4704 1990
Size: 2530 184 3135
Fragments arranged by size:
3135 2530 184
MscI TGG'CCA
Cuts at: 1693 3610 1693
Size: 1917 3932
MslI CAynn'nnrTG
Cuts at: 1375 2027 2696 3838 3965 1375
Size: 652 669 1142 127 3259
Fragments arranged by size:
3259 1142 669 652 127
MspA1I CmG'CkG
Cuts at: 144 166 175 453 698 2257 2960 3274 3634
Size: 22 9 278 245 1559 703 314 360
Cuts at: 3634 4647 4892 144
Size: 1013 245 1101
Fragments arranged by size:
1559 1101 1013 703 360 314 278 245
245 22 9
NaeI GCC'GGC
Cuts at: 4030 4030
Size: 5849
NarI GG'CG_CC
Cuts at: 3280 3528 3280
Size: 248 5601
NcoI C'CATG_G
Cuts at: 2094 2094
Size: 5849
NgoMIV G'CCGG_C
Cuts at: 4028 4028
Size: 5849
NheI G'CTAG_C
Cuts at: 2247 2773 2247
Size: 526 5323
NotI GC'GGCC_GC
Cuts at: 130 176 130
Size: 46 5803
NspI r_CATG'y
Cuts at: 1723 2106 2406 2902 3402 4309 1723
Size: 383 300 496 500 907 3263
Fragments arranged by size:
3263 907 500 496 383 300
PasI CC'CwG_GG
Cuts at: 193 1105 193
Size: 912 4937
PciI A'CATG_T
Cuts at: 2402 4305 2402
Size: 1903 3946
PflMI CCAn_nnn'nTGG
Cuts at: 1700 1700
Size: 5849
PfoI T'CCnGG_A
Cuts at: 476 476
Size: 5849
PmeI GTTT'AAAC
Cuts at: 2848 2848
Size: 5849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4988 5020 4988
Size: 32 5817
PpuMI rG'GwC_Cy
Cuts at: 2367 2367
Size: 5849
PspOMI G'GGCC_C
Cuts at: 202 208 202
Size: 6 5843
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2368 2400 2368
Size: 32 5817
PstI C_TGCA'G
Cuts at: 269 305 2744 2896 3581 269
Size: 36 2439 152 685 2537
Fragments arranged by size:
2537 2439 685 152 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5849
PvuII CAG'CTG
Cuts at: 166 453 2257 3274 3634 166
Size: 287 1804 1017 360 2381
Fragments arranged by size:
2381 1804 1017 360 287
RsrII CG'GwC_CG
Cuts at: 4044 4044
Size: 5849
SacI G_AGCT'C
Cuts at: 576 2863 576
Size: 2287 3562
SalI G'TCGA_C
Cuts at: 361 2886 361
Size: 2525 3324
SapI GCTCTTCn'nnn_
Cuts at: 3872 4082 3872
Size: 210 5639
SbfI CC_TGCA'GG
Cuts at: 2896 2896
Size: 5849
ScaI AGT'ACT
Cuts at: 964 1871 5366 964
Size: 907 3495 1447
Fragments arranged by size:
3495 1447 907
SexAI A'CCwGG_T
Cuts at: 2638 2638
Size: 5849
SfcI C'TryA_G
Cuts at: 33 265 301 321 2008 2589 2623 2740 2892
Size: 232 36 20 1687 581 34 117 152
Cuts at: 2892 3577 4570 4761 33
Size: 685 993 191 1121
Fragments arranged by size:
1687 1121 993 685 581 232 191 152
117 36 34 20
SfoI GGC'GCC
Cuts at: 3281 3529 3281
Size: 248 5601
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5849
SmaI CCC'GGG
Cuts at: 207 2871 207
Size: 2664 3185
SmlI C'TyrA_G
Cuts at: 2607 3004 3919 4411 4673 2607
Size: 397 915 492 262 3783
Fragments arranged by size:
3783 915 492 397 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5849
SphI G_CATG'C
Cuts at: 1723 2902 3402 1723
Size: 1179 500 4170
Fragments arranged by size:
4170 1179 500
SrfI GCCC'GGGC
Cuts at: 207 207
Size: 5849
StyI C'CwwG_G
Cuts at: 1068 1742 2094 2617 2983 3337 1068
Size: 674 352 523 366 354 3580
Fragments arranged by size:
3580 674 523 366 354 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2704 3432 3565 4024 4233 2704
Size: 728 133 459 209 4320
Fragments arranged by size:
4320 728 459 209 133
TatI w'GTAC_w
Cuts at: 962 1869 5364 962
Size: 907 3495 1447
Fragments arranged by size:
3495 1447 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1610 1642 5366 5398 1610
Size: 32 3724 32 2061
Fragments arranged by size:
3724 2061 32 32
Tth111I GACn'n_nGTC
Cuts at: 3646 3646
Size: 5849
XbaI T'CTAG_A
Cuts at: 2397 2880 2397
Size: 483 5366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1240 2516 3344 1240
Size: 1276 828 3745
Fragments arranged by size:
3745 1276 828
XmaI C'CCGG_G
Cuts at: 205 2869 205
Size: 2664 3185
XmnI GAAnn'nnTTC
Cuts at: 530 2320 530
Size: 1790 4059
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AscI AvrII BaeI BcgI
BfrBI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoNI FseI FspAI HpaI NdeI NruI NsiI PacI
PmlI PshAI PsiI PspXI SacII SanDI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI ZraI