(Circular) (Six-base) MAPSORT of: pF1KE2129.seq Check: 624 from: 1 to: 5906 pF1KE2129 5906 bp With 182 enzymes: SgfI * March 14, 2016 17:24 .. AccI GT'mk_AC Cuts at: 2944 2944 Size: 5906 Acc65I G'GTAC_C Cuts at: 2922 2922 Size: 5906 AclI AA'CG_TT Cuts at: 482 5746 482 Size: 5264 642 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 689 1145 1913 2186 2461 2779 3731 4163 4910 Size: 456 768 273 275 318 952 432 747 Cuts at: 4910 5178 689 Size: 268 1417 Fragments arranged by size: 1417 952 768 747 456 432 318 275 273 268 AflIII A'CryG_T Cuts at: 365 3258 4362 5339 365 Size: 2893 1104 977 932 Fragments arranged by size: 2893 1104 977 932 AgeI A'CCGG_T Cuts at: 4294 4294 Size: 5906 AhdI GACnn_n'nnGTC Cuts at: 1477 1477 Size: 5906 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 129 161 129 Size: 32 5874 AlwNI CAG_nnn'CTG Cuts at: 3211 4778 3211 Size: 1567 4339 ApaI G_GGCC'C Cuts at: 134 1660 134 Size: 1526 4380 ApaLI G'TGCA_C Cuts at: 4676 4676 Size: 5906 ApoI r'AATT_y Cuts at: 1234 2200 2335 2540 2910 5259 5515 1234 Size: 966 135 205 370 2349 256 1625 Fragments arranged by size: 2349 1625 966 370 256 205 135 AseI AT'TA_AT Cuts at: 20 929 20 Size: 909 4997 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5906 AvaI C'yCGr_G Cuts at: 152 584 2926 152 Size: 432 2342 3132 Fragments arranged by size: 3132 2342 432 BamHI G'GATC_C Cuts at: 2931 2931 Size: 5906 BanI G'GyrC_C Cuts at: 2406 2922 3336 3584 3619 2406 Size: 516 414 248 35 4693 Fragments arranged by size: 4693 516 414 248 35 BanII G_rGCy'C Cuts at: 121 134 1660 2626 2920 3950 121 Size: 13 1526 966 294 1030 2077 Fragments arranged by size: 2077 1526 1030 966 294 13 BbeI G_GCGC'C Cuts at: 3340 3588 3340 Size: 248 5658 BbsI GAAGACnn'nnnn_ Cuts at: 233 2564 2834 233 Size: 2331 270 3305 Fragments arranged by size: 3305 2331 270 BciVI GTATCCnnnnn_n' Cuts at: 3804 4565 5906 3804 Size: 761 1341 3804 Fragments arranged by size: 3804 1341 761 BclI T'GATC_A Cuts at: 1176 2285 3426 1176 Size: 1109 1141 3656 Fragments arranged by size: 3656 1141 1109 BglII A'GATC_T Cuts at: 919 3421 5458 919 Size: 2502 2037 1367 Fragments arranged by size: 2502 2037 1367 BlpI GC'TnA_GC Cuts at: 3018 3018 Size: 5906 Bme1580I G_kGCm'C Cuts at: 134 1660 2600 3531 3624 4680 134 Size: 1526 940 931 93 1056 1360 Fragments arranged by size: 1526 1360 1056 940 931 93 BmrI ACTGGGnnnn_n' Cuts at: 436 3118 3199 3534 5678 436 Size: 2682 81 335 2144 664 Fragments arranged by size: 2682 2144 664 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4273 4273 Size: 5906 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 694 900 1150 694 Size: 206 250 5450 Fragments arranged by size: 5450 250 206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 550 2766 3082 3961 4453 4751 550 Size: 2216 316 879 492 298 1705 Fragments arranged by size: 2216 1705 879 492 316 298 BsaI GGTCTCn'nnnn_ Cuts at: 5610 5610 Size: 5906 BsaAI yAC'GTr Cuts at: 3259 3259 Size: 5906 BsaBI GATnn'nnATC Cuts at: 6 2636 3420 5457 6 Size: 2630 784 2037 455 Fragments arranged by size: 2630 2037 784 455 BsaHI Gr'CG_yC Cuts at: 3337 3585 4287 5783 3337 Size: 248 702 1496 3460 Fragments arranged by size: 3460 1496 702 248 BsaWI w'CCGG_w Cuts at: 3095 3616 4294 4568 4715 5126 3095 Size: 521 678 274 147 411 3875 Fragments arranged by size: 3875 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 129 159 1435 1465 1817 1847 129 Size: 30 1276 30 352 30 4188 Fragments arranged by size: 4188 1276 352 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 415 2386 415 Size: 1971 3935 BseYI C'CCAG_C Cuts at: 219 3331 4089 4666 5162 219 Size: 3112 758 577 496 963 Fragments arranged by size: 3112 963 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1134 1134 Size: 5906 BsiEI CG_ry'CG Cuts at: 104 1185 3448 3494 4702 104 Size: 1081 2263 46 1208 1308 Fragments arranged by size: 2263 1308 1208 1081 46 BsiHKAI G_wGCw'C Cuts at: 2626 2920 3698 3888 4680 2626 Size: 294 778 190 792 3852 Fragments arranged by size: 3852 792 778 294 190 BsmI GAATG_Cn' Cuts at: 472 516 1491 472 Size: 44 975 4887 Fragments arranged by size: 4887 975 44 Bsp1286I G_dGCh'C Cuts at: 121 134 1660 2600 2626 2920 3531 3624 3698 Size: 13 1526 940 26 294 611 93 74 Cuts at: 3698 3888 3950 4680 121 Size: 190 62 730 1347 Fragments arranged by size: 1526 1347 940 730 611 294 190 93 74 62 26 13 BspEI T'CCGG_A Cuts at: 5126 5126 Size: 5906 BspHI T'CATG_A Cuts at: 852 989 1427 1481 2553 5082 852 Size: 137 438 54 1072 2529 1676 Fragments arranged by size: 2529 1676 1072 438 137 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2956 3170 3472 3853 2956 Size: 214 302 381 5009 Fragments arranged by size: 5009 381 302 214 BsrBI CCG'CTC Cuts at: 50 4198 4252 5736 5901 50 Size: 4148 54 1484 165 55 Fragments arranged by size: 4148 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 879 1017 1730 3818 879 Size: 138 713 2088 2967 Fragments arranged by size: 2967 2088 713 138 BsrFI r'CCGG_y Cuts at: 305 3904 4085 4294 305 Size: 3599 181 209 1917 Fragments arranged by size: 3599 1917 209 181 BssSI C'ACGA_G Cuts at: 4177 4535 4177 Size: 358 5548 BstAPI GCAn_nnn'nTGC Cuts at: 878 3283 878 Size: 2405 3501 BstBI TT'CG_AA Cuts at: 4267 4267 Size: 5906 BstXI CCAn_nnnn'nTGG Cuts at: 1467 1467 Size: 5906 BstYI r'GATC_y Cuts at: 1 283 919 981 2931 3398 3421 3756 4002 Size: 282 636 62 1950 467 23 335 246 Cuts at: 4002 5014 5100 5112 5458 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1950 1012 636 467 449 346 335 282 246 86 62 23 12 BtgI C'CryG_G Cuts at: 2870 2870 Size: 5906 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3856 4037 4200 3856 Size: 181 163 5562 Fragments arranged by size: 5562 181 163 BtsI GCAGTG_nn' Cuts at: 533 789 1084 3149 533 Size: 256 295 2065 3290 Fragments arranged by size: 3290 2065 295 256 DraI TTT'AAA Cuts at: 1205 2905 1205 Size: 1700 4206 DrdI GACnn_nn'nnGTC Cuts at: 3612 4470 3612 Size: 858 5048 EaeI y'GGCC_r Cuts at: 530 1028 1850 3491 3665 4056 4083 5428 530 Size: 498 822 1641 174 391 27 1345 1008 Fragments arranged by size: 1641 1345 1008 822 498 391 174 27 EagI C'GGCC_G Cuts at: 3491 3491 Size: 5906 EarI CTCTTCn'nnn_ Cuts at: 2117 3929 4139 2117 Size: 1812 210 3884 Fragments arranged by size: 3884 1812 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4114 4424 4570 5716 4114 Size: 310 146 1146 4304 Fragments arranged by size: 4304 1146 310 146 EcoICRI GAG'CTC Cuts at: 2624 2918 2624 Size: 294 5612 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 689 694 900 1145 1150 1913 2186 2461 2779 Size: 5 206 245 5 763 273 275 318 Cuts at: 2779 3731 4163 4910 5178 689 Size: 952 432 747 268 1417 Fragments arranged by size: 1417 952 763 747 432 318 275 273 268 245 206 5 5 EcoNI CCTnn'n_nnAGG Cuts at: 826 826 Size: 5906 EcoO109I rG'GnC_Cy Cuts at: 131 1656 1657 1866 3045 131 Size: 1525 1 209 1179 2992 Fragments arranged by size: 2992 1525 1179 209 1 EcoRI G'AATT_C Cuts at: 2910 2910 Size: 5906 EcoRV GAT'ATC Cuts at: 1982 1982 Size: 5906 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 974 3925 974 Size: 2951 2955 FspI TGC'GCA Cuts at: 3687 3687 Size: 5906 HaeII r_GCGC'y Cuts at: 161 3340 3588 4610 5589 161 Size: 3179 248 1022 979 478 Fragments arranged by size: 3179 1022 979 478 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1161 1193 1463 1495 2851 2883 3748 3778 3780 Size: 32 270 32 1356 32 865 30 2 Cuts at: 3780 3810 1161 Size: 30 3257 Fragments arranged by size: 3257 1356 865 270 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 486 2945 486 Size: 2459 3447 HindIII A'AGCT_T Cuts at: 1962 2443 1962 Size: 481 5425 HpaI GTT'AAC Cuts at: 486 486 Size: 5906 KasI G'GCGC_C Cuts at: 3336 3584 3336 Size: 248 5658 KpnI G_GTAC'C Cuts at: 2926 2926 Size: 5906 MfeI C'AATT_G Cuts at: 1907 1907 Size: 5906 MluI A'CGCG_T Cuts at: 5339 5339 Size: 5906 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1077 4577 4761 1077 Size: 3500 184 2222 Fragments arranged by size: 3500 2222 184 MscI TGG'CCA Cuts at: 532 1030 1852 3667 532 Size: 498 822 1815 2771 Fragments arranged by size: 2771 1815 822 498 MslI CAynn'nnrTG Cuts at: 442 2035 3895 4022 442 Size: 1593 1860 127 2326 Fragments arranged by size: 2326 1860 1593 127 MspA1I CmG'CkG Cuts at: 172 249 424 1492 1554 3017 3331 3691 4704 Size: 77 175 1068 62 1463 314 360 1013 Cuts at: 4704 4949 172 Size: 245 1129 Fragments arranged by size: 1463 1129 1068 1013 360 314 245 175 77 62 NaeI GCC'GGC Cuts at: 4087 4087 Size: 5906 NarI GG'CG_CC Cuts at: 3337 3585 3337 Size: 248 5658 NcoI C'CATG_G Cuts at: 2870 2870 Size: 5906 NgoMIV G'CCGG_C Cuts at: 4085 4085 Size: 5906 NspI r_CATG'y Cuts at: 369 1692 1759 2959 3459 4366 369 Size: 1323 67 1200 500 907 1909 Fragments arranged by size: 1909 1323 1200 907 500 67 PciI A'CATG_T Cuts at: 365 4362 365 Size: 3997 1909 PflMI CCAn_nnn'nTGG Cuts at: 2354 2354 Size: 5906 PfoI T'CCnGG_A Cuts at: 2300 2492 2300 Size: 192 5714 PmeI GTTT'AAAC Cuts at: 2905 2905 Size: 5906 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 129 161 548 580 1030 1062 5045 5077 129 Size: 32 387 32 450 32 3983 32 958 Fragments arranged by size: 3983 958 450 387 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 1866 1866 Size: 5906 PspOMI G'GGCC_C Cuts at: 130 1656 130 Size: 1526 4380 PstI C_TGCA'G Cuts at: 1979 2714 2953 3638 1979 Size: 735 239 685 4247 Fragments arranged by size: 4247 735 685 239 PvuI CG_AT'CG Cuts at: 104 104 Size: 5906 PvuII CAG'CTG Cuts at: 424 1492 1554 3331 3691 424 Size: 1068 62 1777 360 2639 Fragments arranged by size: 2639 1777 1068 360 62 RsrII CG'GwC_CG Cuts at: 4101 4101 Size: 5906 SacI G_AGCT'C Cuts at: 2626 2920 2626 Size: 294 5612 SalI G'TCGA_C Cuts at: 2943 2943 Size: 5906 SanDI GG'GwC_CC Cuts at: 1866 1866 Size: 5906 SapI GCTCTTCn'nnn_ Cuts at: 3929 4139 3929 Size: 210 5696 SbfI CC_TGCA'GG Cuts at: 2953 2953 Size: 5906 ScaI AGT'ACT Cuts at: 5423 5423 Size: 5906 SfcI C'TryA_G Cuts at: 33 395 517 1913 1975 2710 2949 3634 4627 Size: 362 122 1396 62 735 239 685 993 Cuts at: 4627 4818 33 Size: 191 1121 Fragments arranged by size: 1396 1121 993 735 685 362 239 191 122 62 SfoI GGC'GCC Cuts at: 3338 3586 3338 Size: 248 5658 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5906 SgrAI Cr'CCGG_yG Cuts at: 305 305 Size: 5906 SmaI CCC'GGG Cuts at: 154 2928 154 Size: 2774 3132 SmlI C'TyrA_G Cuts at: 565 584 2781 3061 3976 4468 4730 565 Size: 19 2197 280 915 492 262 1741 Fragments arranged by size: 2197 1741 915 492 280 262 19 SpeI A'CTAG_T Cuts at: 67 67 Size: 5906 SphI G_CATG'C Cuts at: 1759 2959 3459 1759 Size: 1200 500 4206 Fragments arranged by size: 4206 1200 500 SspI AAT'ATT Cuts at: 1035 1669 1035 Size: 634 5272 StuI AGG'CCT Cuts at: 944 944 Size: 5906 StyI C'CwwG_G Cuts at: 1661 2870 3040 3394 1661 Size: 1209 170 354 4173 Fragments arranged by size: 4173 1209 354 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3489 3622 4081 4290 3489 Size: 133 459 209 5105 Fragments arranged by size: 5105 459 209 133 TatI w'GTAC_w Cuts at: 2706 5421 2706 Size: 2715 3191 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1435 1467 1817 1849 5423 5455 1435 Size: 32 350 32 3574 32 1886 Fragments arranged by size: 3574 1886 350 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3703 3703 Size: 5906 XbaI T'CTAG_A Cuts at: 2937 2937 Size: 5906 XcmI CCAnnnn_n'nnnnTGG Cuts at: 540 3401 540 Size: 2861 3045 XhoI C'TCGA_G Cuts at: 584 584 Size: 5906 XmaI C'CCGG_G Cuts at: 152 2926 152 Size: 2774 3132 XmnI GAAnn'nnTTC Cuts at: 560 644 1045 2633 560 Size: 84 401 1588 3833 Fragments arranged by size: 3833 1588 401 84 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI SnaBI SrfI SwaI ZraI