Restriction map for
pF1KE2188
(Circular) (Six-base) MAPSORT of: pF1KE2188.seq Check: 5270 from: 1 to: 4382
pF1KE2188 4382 bp
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AccI GT'mk_AC
Cuts at: 231 782 1096 1420 231
Size: 551 314 324 3193
Fragments arranged by size:
3193 551 324 314
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 670 741 2207 2639 3386 3654 345
Size: 325 71 1466 432 747 268 1073
Fragments arranged by size:
1466 1073 747 432 325 268 71
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AleI CACnn'nnGTG
Cuts at: 886 1275 886
Size: 389 3993
AlwNI CAG_nnn'CTG
Cuts at: 316 379 1687 3254 316
Size: 63 1308 1567 1444
Fragments arranged by size:
1567 1444 1308 63
ApaI G_GGCC'C
Cuts at: 132 971 132
Size: 839 3543
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 1124 1386 3735 3991 1124
Size: 262 2349 256 1515
Fragments arranged by size:
2349 1515 262 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 132 154 419 971 1396 2426 132
Size: 22 265 552 425 1030 2088
Fragments arranged by size:
2088 1030 552 425 265 22
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 129 129
Size: 4382
BbvCI CC'TCA_GC
Cuts at: 467 467
Size: 4382
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglII A'GATC_T
Cuts at: 1265 1897 3934 1265
Size: 632 2037 1713
Fragments arranged by size:
2037 1713 632
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 132 971 2007 2100 3156 132
Size: 839 1036 93 1056 1358
Fragments arranged by size:
1358 1056 1036 839 93
BmgBI CAC'GTC
Cuts at: 135 135
Size: 4382
BmrI ACTGGGnnnn_n'
Cuts at: 1062 1207 1594 1675 2010 4154 1062
Size: 145 387 81 335 2144 1290
Fragments arranged by size:
2144 1290 387 335 145 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1060 1207 1332 475
Size: 585 147 125 3525
Fragments arranged by size:
3525 585 147 125
Bpu10I CC'TnA_GC
Cuts at: 467 1049 1295 467
Size: 582 246 3554
Fragments arranged by size:
3554 582 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 804 1558 2437 2929 3227 613
Size: 191 754 879 492 298 1768
Fragments arranged by size:
1768 879 754 492 298 191
BsaI GGTCTCn'nnnn_
Cuts at: 723 4086 723
Size: 3363 1019
BsaAI yAC'GTr
Cuts at: 884 1735 884
Size: 851 3531
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1083 1813 2061 2763 4259 1083
Size: 730 248 702 1496 1206
Fragments arranged by size:
1496 1206 730 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 925 955 1305 1335 925
Size: 30 350 30 3972
Fragments arranged by size:
3972 350 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 484 259
Size: 225 4157
BseYI C'CCAG_C
Cuts at: 203 605 814 854 923 1191 1807 2565 3142
Size: 402 209 40 69 268 616 758 577
Cuts at: 3142 3638 203
Size: 496 947
Fragments arranged by size:
947 758 616 577 496 402 268 209
69 40
BsiEI CG_ry'CG
Cuts at: 104 164 986 1924 1970 3178 104
Size: 60 822 938 46 1208 1308
Fragments arranged by size:
1308 1208 938 822 60 46
BsiHKAI G_wGCw'C
Cuts at: 872 1396 2174 2364 3156 872
Size: 524 778 190 792 2098
Fragments arranged by size:
2098 792 778 524 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1090 1090
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 132 154 419 872 971 1396 2007 2100 2174
Size: 22 265 453 99 425 611 93 74
Cuts at: 2174 2364 2426 3156 132
Size: 190 62 730 1358
Fragments arranged by size:
1358 730 611 453 425 265 190 99
93 74 62 22
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1346 2674 2728 4212 4377 50
Size: 1296 1328 54 1484 165 55
Fragments arranged by size:
1484 1328 1296 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstEII G'GTnAC_C
Cuts at: 327 327
Size: 4382
BstYI r'GATC_y
Cuts at: 1 1265 1407 1874 1897 2232 2478 3490 3576
Size: 1264 142 467 23 335 246 1012 86
Cuts at: 3576 3588 3934 1
Size: 12 346 449
Fragments arranged by size:
1264 1012 467 449 346 335 246 142
86 23 12
BtgI C'CryG_G
Cuts at: 107 509 107
Size: 402 3980
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 900 2332 2513 2676 900
Size: 1432 181 163 2606
Fragments arranged by size:
2606 1432 181 163
BtsI GCAGTG_nn'
Cuts at: 579 687 1625 579
Size: 108 938 3336
Fragments arranged by size:
3336 938 108
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 301 545 725 1284 1967 2141 2532 2559 3904
Size: 244 180 559 683 174 391 27 1345
Cuts at: 3904 301
Size: 779
Fragments arranged by size:
1345 779 683 559 391 244 180 174
27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 544 877 2405 2615 544
Size: 333 1528 210 2311
Fragments arranged by size:
2311 1528 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1165 2590 2900 3046 4192 1165
Size: 1425 310 146 1146 1355
Fragments arranged by size:
1425 1355 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 475 670 741 1060 1207 1332 2207 2639
Size: 130 195 71 319 147 125 875 432
Cuts at: 2639 3386 3654 345
Size: 747 268 1073
Fragments arranged by size:
1073 875 747 432 319 268 195 147
130 125 71
EcoO109I rG'GnC_Cy
Cuts at: 128 927 967 1316 1521 128
Size: 799 40 349 205 2989
Fragments arranged by size:
2989 799 349 205 40
EcoRI G'AATT_C
Cuts at: 1124 1386 1124
Size: 262 4120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 337 369 460 492 925 957 2224
Size: 32 92 32 91 32 433 32 1267
Cuts at: 2224 2254 2256 2286 213
Size: 30 2 30 2309
Fragments arranged by size:
2309 1267 433 92 91 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1421 1097
Size: 324 4058
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 303 547 1286 2143 303
Size: 244 739 857 2542
Fragments arranged by size:
2542 857 739 244
MslI CAynn'nnrTG
Cuts at: 886 1027 1275 1286 1371 2371 2498 886
Size: 141 248 11 85 1000 127 2770
Fragments arranged by size:
2770 1000 248 141 127 85 11
MspA1I CmG'CkG
Cuts at: 207 609 761 1493 1807 2167 3180 3425 207
Size: 402 152 732 314 360 1013 245 1164
Fragments arranged by size:
1164 1013 732 402 360 314 245 152
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 180 1435 1935 2842 180
Size: 1255 500 907 1720
Fragments arranged by size:
1720 1255 907 500
PasI CC'CwG_GG
Cuts at: 264 264
Size: 4382
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 1120 1120
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PmlI CAC'GTG
Cuts at: 884 884
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PpuMI rG'GwC_Cy
Cuts at: 927 1316 927
Size: 389 3993
PspOMI G'GGCC_C
Cuts at: 128 967 128
Size: 839 3543
PstI C_TGCA'G
Cuts at: 694 1429 2114 694
Size: 735 685 2962
Fragments arranged by size:
2962 735 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 207 609 761 1807 2167 207
Size: 402 152 1046 360 2422
Fragments arranged by size:
2422 1046 402 360 152
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1095 1419 1095
Size: 324 4058
SapI GCTCTTCn'nnn_
Cuts at: 877 2405 2615 877
Size: 1528 210 2644
Fragments arranged by size:
2644 1528 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 690 1425 2110 3103 3294 33
Size: 657 735 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 657 191
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 628 819 1537 2452 2944 3206 628
Size: 191 718 915 492 262 1804
Fragments arranged by size:
1804 915 718 492 262 191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
SspI AAT'ATT
Cuts at: 1014 1014
Size: 4382
StyI C'CwwG_G
Cuts at: 107 1516 1870 107
Size: 1409 354 2619
Fragments arranged by size:
2619 1409 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 197 217 619 876 972 1965 2098 2557 2766
Size: 20 402 257 96 993 133 459 209
Cuts at: 2766 197
Size: 1813
Fragments arranged by size:
1813 993 459 402 257 209 133 96
20
TatI w'GTAC_w
Cuts at: 394 3897 394
Size: 3503 879
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 904 936 1305 1337 3899 3931 904
Size: 32 369 32 2562 32 1355
Fragments arranged by size:
2562 1355 369 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 555 1877 261
Size: 294 1322 2766
Fragments arranged by size:
2766 1322 294
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsgI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI