Restriction map for
pF1KE2200
(Circular) (Six-base) MAPSORT of: pF1KE2200.seq Check: 5879 from: 1 to: 4664
pF1KE2200 4664 bp
With 182 enzymes: SgfI *
November 26, 2010 10:36 ..
AccI GT'mk_AC
Cuts at: 1702 1702
Size: 4664
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1176 1525 1536 2489 2921 3668 3936 1176
Size: 349 11 953 432 747 268 1904
Fragments arranged by size:
1904 953 747 432 349 268 11
AfeI AGC'GCT
Cuts at: 255 255
Size: 4664
AflIII A'CryG_T
Cuts at: 127 1215 2016 3120 4097 127
Size: 1088 801 1104 977 694
Fragments arranged by size:
1104 1088 977 801 694
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AleI CACnn'nnGTG
Cuts at: 1355 1355
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 576 1969 3536 576
Size: 1393 1567 1704
Fragments arranged by size:
1704 1567 1393
ApaI G_GGCC'C
Cuts at: 644 644
Size: 4664
ApaLI G'TGCA_C
Cuts at: 3434 3434
Size: 4664
ApoI r'AATT_y
Cuts at: 1668 4017 4273 1668
Size: 2349 256 2059
Fragments arranged by size:
2349 2059 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 213 438 724 1684 213
Size: 225 286 960 3193
Fragments arranged by size:
3193 960 286 225
BamHI G'GATC_C
Cuts at: 849 1689 849
Size: 840 3824
BanI G'GyrC_C
Cuts at: 176 238 414 894 1680 2094 2342 2377 176
Size: 62 176 480 786 414 248 35 2463
Fragments arranged by size:
2463 786 480 414 248 176 62 35
BanII G_rGCy'C
Cuts at: 319 644 871 1075 1164 1678 2708 319
Size: 325 227 204 89 514 1030 2275
Fragments arranged by size:
2275 1030 514 325 227 204 89
BbeI G_GCGC'C
Cuts at: 242 418 2098 2346 242
Size: 176 1680 248 2560
Fragments arranged by size:
2560 1680 248 176
BbsI GAAGACnn'nnnn_
Cuts at: 1182 1513 1182
Size: 331 4333
BbvCI CC'TCA_GC
Cuts at: 961 961
Size: 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4630
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3323 4664 2562
Size: 761 1341 2562
Fragments arranged by size:
2562 1341 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BfrBI ATG'CAT
Cuts at: 1016 1623 1016
Size: 607 4057
BglI GCCn_nnn'nGGC
Cuts at: 247 866 247
Size: 619 4045
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 1776 1776
Size: 4664
Bme1580I G_kGCm'C
Cuts at: 387 644 2289 2382 3438 387
Size: 257 1645 93 1056 1613
Fragments arranged by size:
1645 1613 1056 257 93
BmgBI CAC'GTC
Cuts at: 324 1216 324
Size: 892 3772
BmrI ACTGGGnnnn_n'
Cuts at: 1876 1957 2292 4436 1876
Size: 81 335 2144 2104
Fragments arranged by size:
2144 2104 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1059 1244 1630 250
Size: 809 185 386 3284
Fragments arranged by size:
3284 809 386 185
Bpu10I CC'TnA_GC
Cuts at: 577 961 577
Size: 384 4280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1840 2719 3211 3509 1840
Size: 879 492 298 2995
Fragments arranged by size:
2995 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1252 4368 1252
Size: 3116 1548
BsaAI yAC'GTr
Cuts at: 128 2017 128
Size: 1889 2775
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 145 239 289 415 670 781 967 2095 2343
Size: 94 50 126 255 111 186 1128 248
Cuts at: 2343 3045 4541 145
Size: 702 1496 268
Fragments arranged by size:
1496 1128 702 268 255 248 186 126
111 94 50
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3326 3473 3884 1853
Size: 521 678 274 147 411 2633
Fragments arranged by size:
2633 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 130
Size: 30 4634
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 291 580 644 291
Size: 289 64 4311
Fragments arranged by size:
4311 289 64
BseYI C'CCAG_C
Cuts at: 542 661 715 784 873 1004 1066 2089 2847
Size: 119 54 69 89 131 62 1023 758
Cuts at: 2847 3424 3920 542
Size: 577 496 1286
Fragments arranged by size:
1286 1023 758 577 496 131 119 89
69 62 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 921
Size: 4664
BsiEI CG_ry'CG
Cuts at: 104 1249 2206 2252 3460 104
Size: 1145 957 46 1208 1308
Fragments arranged by size:
1308 1208 1145 957 46
BsiHKAI G_wGCw'C
Cuts at: 1075 1678 2456 2646 3438 1075
Size: 603 778 190 792 2301
Fragments arranged by size:
2301 792 778 603 190
BsmI GAATG_Cn'
Cuts at: 1018 1199 1018
Size: 181 4483
BsmBI CGTCTCn'nnnn_
Cuts at: 152 268 152
Size: 116 4548
Bsp1286I G_dGCh'C
Cuts at: 319 387 644 871 1075 1164 1678 2289 2382
Size: 68 257 227 204 89 514 611 93
Cuts at: 2382 2456 2646 2708 3438 319
Size: 74 190 62 730 1545
Fragments arranged by size:
1545 730 611 514 257 227 204 190
93 89 74 68 62
BspEI T'CCGG_A
Cuts at: 3884 3884
Size: 4664
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 719 1714 1928 2230 2611 719
Size: 995 214 302 381 2772
Fragments arranged by size:
2772 995 381 302 214
BsrBI CCG'CTC
Cuts at: 50 164 398 2956 3010 4494 4659 50
Size: 114 234 2558 54 1484 165 55
Fragments arranged by size:
2558 1484 234 165 114 55 54
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 141 417 483 940 2662 2843 3052 141
Size: 276 66 457 1722 181 209 1753
Fragments arranged by size:
1753 1722 457 276 209 181 66
BssSI C'ACGA_G
Cuts at: 562 2935 3293 562
Size: 2373 358 1933
Fragments arranged by size:
2373 1933 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 765 765
Size: 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 613 613
Size: 4664
BstYI r'GATC_y
Cuts at: 1 849 1689 2156 2179 2514 2760 3772 3858
Size: 848 840 467 23 335 246 1012 86
Cuts at: 3858 3870 4216 1
Size: 12 346 449
Fragments arranged by size:
1012 848 840 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 282 854 282
Size: 572 4092
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2614 2795 2958 2614
Size: 181 163 4320
Fragments arranged by size:
4320 181 163
BtsI GCAGTG_nn'
Cuts at: 415 577 984 1907 415
Size: 162 407 923 3172
Fragments arranged by size:
3172 923 407 162
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 603 678 1564 2249 2423 2814 2841 4186 603
Size: 75 886 685 174 391 27 1345 1081
Fragments arranged by size:
1345 1081 886 685 391 174 75 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 479 647 1597 2872 3182 3328 4474 479
Size: 168 950 1275 310 146 1146 669
Fragments arranged by size:
1275 1146 950 669 310 168 146
EcoICRI GAG'CTC
Cuts at: 1073 1676 1073
Size: 603 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1059 1176 1244 1525 1536 1630 2489 2921
Size: 809 117 68 281 11 94 859 432
Cuts at: 2921 3668 3936 250
Size: 747 268 978
Fragments arranged by size:
978 859 809 747 432 281 268 117
94 68 11
EcoO109I rG'GnC_Cy
Cuts at: 114 210 330 641 1803 114
Size: 96 120 311 1162 2975
Fragments arranged by size:
2975 1162 311 120 96
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 242 257 418 446 584 1657 2098 2346 3368
Size: 15 161 28 138 1073 441 248 1022
Cuts at: 3368 4347 242
Size: 979 559
Fragments arranged by size:
1073 1022 979 559 441 248 161 138
28 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 1011 1043 2506 2536 2538 2568 130
Size: 32 849 32 1463 30 2 30 2226
Fragments arranged by size:
2226 1463 849 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1703 1703
Size: 4664
KasI G'GCGC_C
Cuts at: 238 414 2094 2342 238
Size: 176 1680 248 2560
Fragments arranged by size:
2560 1680 248 176
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 1520 3335 3519 344
Size: 1176 1815 184 1489
Fragments arranged by size:
1815 1489 1176 184
MscI TGG'CCA
Cuts at: 1566 2425 1566
Size: 859 3805
MslI CAynn'nnrTG
Cuts at: 1355 1371 1445 1461 2653 2780 1355
Size: 16 74 16 1192 127 3239
Fragments arranged by size:
3239 1192 127 74 16 16
MspA1I CmG'CkG
Cuts at: 284 719 935 1556 1650 1775 2089 2449 3462
Size: 435 216 621 94 125 314 360 1013
Cuts at: 3462 3707 284
Size: 245 1241
Fragments arranged by size:
1241 1013 621 435 360 314 245 216
125 94
NaeI GCC'GGC
Cuts at: 143 2845 143
Size: 2702 1962
NarI GG'CG_CC
Cuts at: 239 415 2095 2343 239
Size: 176 1680 248 2560
Fragments arranged by size:
2560 1680 248 176
NcoI C'CATG_G
Cuts at: 854 854
Size: 4664
NgoMIV G'CCGG_C
Cuts at: 141 2843 141
Size: 2702 1962
NsiI A_TGCA'T
Cuts at: 1018 1625 1018
Size: 607 4057
NspI r_CATG'y
Cuts at: 1623 1717 2217 3124 1623
Size: 94 500 907 3163
Fragments arranged by size:
3163 907 500 94
PasI CC'CwG_GG
Cuts at: 363 363
Size: 4664
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PfoI T'CCnGG_A
Cuts at: 134 206 134
Size: 72 4592
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PmlI CAC'GTG
Cuts at: 128 128
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 114 210 330 114
Size: 96 120 4448
Fragments arranged by size:
4448 120 96
PspOMI G'GGCC_C
Cuts at: 640 640
Size: 4664
PstI C_TGCA'G
Cuts at: 1711 2396 1711
Size: 685 3979
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 719 935 1556 1650 2089 2449 719
Size: 216 621 94 439 360 2934
Fragments arranged by size:
2934 621 439 360 216 94
RsrII CG'GwC_CG
Cuts at: 1493 2859 1493
Size: 1366 3298
SacI G_AGCT'C
Cuts at: 1075 1678 1075
Size: 603 4061
SacII CC_GC'GG
Cuts at: 285 285
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SanDI GG'GwC_CC
Cuts at: 210 210
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 889 4181 889
Size: 3292 1372
SfcI C'TryA_G
Cuts at: 33 181 972 1707 2392 3385 3576 33
Size: 148 791 735 685 993 191 1121
Fragments arranged by size:
1121 993 791 735 685 191 148
SfoI GGC'GCC
Cuts at: 240 416 2096 2344 240
Size: 176 1680 248 2560
Fragments arranged by size:
2560 1680 248 176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SgrAI Cr'CCGG_yG
Cuts at: 417 417
Size: 4664
SmaI CCC'GGG
Cuts at: 215 440 1686 215
Size: 225 1246 3193
Fragments arranged by size:
3193 1246 225
SmlI C'TyrA_G
Cuts at: 724 1819 2734 3226 3488 724
Size: 1095 915 492 262 1900
Fragments arranged by size:
1900 1095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
StyI C'CwwG_G
Cuts at: 854 1798 2152 854
Size: 944 354 3366
Fragments arranged by size:
3366 944 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 355 637 865 2247 2380 2839 3048 164
Size: 191 282 228 1382 133 459 209 1780
Fragments arranged by size:
1780 1382 459 282 228 209 191 133
TatI w'GTAC_w
Cuts at: 887 4179 887
Size: 3292 1372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4181 4213 4181
Size: 32 4632
Tth111I GACn'n_nGTC
Cuts at: 2461 2461
Size: 4664
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1647 2159 1647
Size: 512 4152
XhoI C'TCGA_G
Cuts at: 724 724
Size: 4664
XmaI C'CCGG_G
Cuts at: 213 438 1684 213
Size: 225 1246 3193
Fragments arranged by size:
3193 1246 225
XmnI GAAnn'nnTTC
Cuts at: 1401 1456 1401
Size: 55 4609
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PflMI PshAI
PsiI PspXI PsrI SexAI SfiI SnaBI SrfI SspI
StuI SwaI ZraI