Restriction map for
pF1KE2256
(Circular) (Six-base) MAPSORT of: pF1KE2256.seq Check: 8397 from: 1 to: 4370
pF1KE2256 4370 bp
With 182 enzymes: SgfI *
July 20, 2018 17:51 ..
AatII G_ACGT'C
Cuts at: 455 455
Size: 4370
AccI GT'mk_AC
Cuts at: 383 689 1408 383
Size: 306 719 3345
Fragments arranged by size:
3345 719 306
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2627 3374 3642 2195
Size: 432 747 268 2923
Fragments arranged by size:
2923 747 432 268
AfeI AGC'GCT
Cuts at: 769 769
Size: 4370
AflIII A'CryG_T
Cuts at: 1722 2826 3803 1722
Size: 1104 977 2289
Fragments arranged by size:
2289 1104 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1366 1366
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 567 1218 1675 3242 567
Size: 651 457 1567 1695
Fragments arranged by size:
1695 1567 651 457
ApaLI G'TGCA_C
Cuts at: 462 1201 3140 462
Size: 739 1939 1692
Fragments arranged by size:
1939 1692 739
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 917 1390 917
Size: 473 3897
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 577 609 1386 1800 2048 2083 577
Size: 32 777 414 248 35 2864
Fragments arranged by size:
2864 777 414 248 35 32
BanII G_rGCy'C
Cuts at: 421 1384 2414 421
Size: 963 1030 2377
Fragments arranged by size:
2377 1030 963
BbeI G_GCGC'C
Cuts at: 581 613 1804 2052 581
Size: 32 1191 248 2899
Fragments arranged by size:
2899 1191 248 32
BbsI GAAGACnn'nnnn_
Cuts at: 1305 1329 1305
Size: 24 4346
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3029 4370 2268
Size: 761 1341 2268
Fragments arranged by size:
2268 1341 761
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglI GCCn_nnn'nGGC
Cuts at: 169 442 1245 169
Size: 273 803 3294
Fragments arranged by size:
3294 803 273
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 415 1482 415
Size: 1067 3303
Bme1580I G_kGCm'C
Cuts at: 466 1205 1241 1995 2088 3144 466
Size: 739 36 754 93 1056 1692
Fragments arranged by size:
1692 1056 754 739 93 36
BmgBI CAC'GTC
Cuts at: 636 784 636
Size: 148 4222
BmrI ACTGGGnnnn_n'
Cuts at: 753 1582 1663 1998 4142 753
Size: 829 81 335 2144 981
Fragments arranged by size:
2144 981 829 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 688
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 2917 3215 1546
Size: 879 492 298 2701
Fragments arranged by size:
2701 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4074 4074
Size: 4370
BsaAI yAC'GTr
Cuts at: 584 684 1723 584
Size: 100 1039 3231
Fragments arranged by size:
3231 1039 100
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 307 434 452 578 610 1049 1186 1801 2049
Size: 127 18 126 32 439 137 615 248
Cuts at: 2049 2751 4247 307
Size: 702 1496 430
Fragments arranged by size:
1496 702 615 439 430 248 137 127
126 32 18
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1092 1122 1092
Size: 30 4340
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 343 340
Size: 3 4367
BseYI C'CCAG_C
Cuts at: 148 199 770 820 955 1269 1795 2553 3130
Size: 51 571 50 135 314 526 758 577
Cuts at: 3130 3626 148
Size: 496 892
Fragments arranged by size:
892 758 577 571 526 496 314 135
51 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 781
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 164 341 645 804 1125 1912 1958 3166
Size: 60 177 304 159 321 787 46 1208
Cuts at: 3166 104
Size: 1308
Fragments arranged by size:
1308 1208 787 321 304 177 159 60
46
BsiHKAI G_wGCw'C
Cuts at: 254 466 706 1041 1205 1384 2162 2352 3144
Size: 212 240 335 164 179 778 190 792
Cuts at: 3144 254
Size: 1480
Fragments arranged by size:
1480 792 778 335 240 212 190 179
164
Bsp1286I G_dGCh'C
Cuts at: 254 421 466 706 1041 1205 1241 1384 1995
Size: 167 45 240 335 164 36 143 611
Cuts at: 1995 2088 2162 2352 2414 3144 254
Size: 93 74 190 62 730 1480
Fragments arranged by size:
1480 730 611 335 240 190 167 164
143 93 74 62 45 36
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 3546 3546
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1420 1634 1936 2317 1420
Size: 214 302 381 3473
Fragments arranged by size:
3473 381 302 214
BsrBI CCG'CTC
Cuts at: 50 375 726 2662 2716 4200 4365 50
Size: 325 351 1936 54 1484 165 55
Fragments arranged by size:
1936 1484 351 325 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 159 539 557 1120 1152 2368 2549 2758 159
Size: 380 18 563 32 1216 181 209 1771
Fragments arranged by size:
1771 1216 563 380 209 181 32 18
BssHII G'CGCG_C
Cuts at: 294 806 294
Size: 512 3858
BssSI C'ACGA_G
Cuts at: 268 2641 2999 268
Size: 2373 358 1639
Fragments arranged by size:
2373 1639 358
BstAPI GCAn_nnn'nTGC
Cuts at: 567 1747 567
Size: 1180 3190
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstXI CCAn_nnnn'nTGG
Cuts at: 498 498
Size: 4370
BstYI r'GATC_y
Cuts at: 1 1395 1862 1885 2220 2466 3478 3564 3576
Size: 1394 467 23 335 246 1012 86 12
Cuts at: 3576 3922 1
Size: 346 449
Fragments arranged by size:
1394 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 125 164 446 515 596 756 1243 107
Size: 18 39 282 69 81 160 487 3234
Fragments arranged by size:
3234 487 282 160 81 69 39 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 1613 1613
Size: 4370
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 685 2076 2934 685
Size: 1391 858 2121
Fragments arranged by size:
2121 1391 858
EaeI y'GGCC_r
Cuts at: 161 284 338 443 512 518 642 801 1122
Size: 123 54 105 69 6 124 159 321
Cuts at: 1122 1955 2129 2520 2547 3892 161
Size: 833 174 391 27 1345 639
Fragments arranged by size:
1345 833 639 391 321 174 159 124
123 105 69 54 27 6
EagI C'GGCC_G
Cuts at: 161 338 642 801 1122 1955 161
Size: 177 304 159 321 833 2576
Fragments arranged by size:
2576 833 321 304 177 159
EarI CTCTTCn'nnn_
Cuts at: 382 2393 2603 382
Size: 2011 210 2149
Fragments arranged by size:
2149 2011 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 2195 2627 3374 3642 688
Size: 1507 432 747 268 1416
Fragments arranged by size:
1507 1416 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 868 964 868
Size: 96 4274
EcoO109I rG'GnC_Cy
Cuts at: 873 922 969 1146 1166 1347 1509 873
Size: 49 47 177 20 181 162 3734
Fragments arranged by size:
3734 181 177 162 49 47 20
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4370
FseI GG_CCGG'CC
Cuts at: 163 163
Size: 4370
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 220 581 613 771 1219 1259 1289 1804 2052
Size: 361 32 158 448 40 30 515 248
Cuts at: 2052 3074 4053 220
Size: 1022 979 537
Fragments arranged by size:
1022 979 537 515 448 361 248 158
40 32 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2212 2242 2244 2274 2212
Size: 30 2 30 4308
Fragments arranged by size:
4308 30 30 2
HincII GTy'rAC
Cuts at: 1281 1409 1281
Size: 128 4242
KasI G'GCGC_C
Cuts at: 577 609 1800 2048 577
Size: 32 1191 248 2899
Fragments arranged by size:
2899 1191 248 32
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1055 3041 3225 1055
Size: 1986 184 2200
Fragments arranged by size:
2200 1986 184
MscI TGG'CCA
Cuts at: 286 445 2131 286
Size: 159 1686 2525
Fragments arranged by size:
2525 1686 159
MslI CAynn'nnrTG
Cuts at: 445 496 2359 2486 445
Size: 51 1863 127 2329
Fragments arranged by size:
2329 1863 127 51
MspA1I CmG'CkG
Cuts at: 127 166 528 564 598 758 824 1245 1481
Size: 39 362 36 34 160 66 421 236
Cuts at: 1481 1795 2155 3168 3413 127
Size: 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 421 362 360 314 245 236
160 66 39 36 34
NaeI GCC'GGC
Cuts at: 161 559 2551 161
Size: 398 1992 1980
Fragments arranged by size:
1992 1980 398
NarI GG'CG_CC
Cuts at: 578 610 1801 2049 578
Size: 32 1191 248 2899
Fragments arranged by size:
2899 1191 248 32
NcoI C'CATG_G
Cuts at: 107 446 107
Size: 339 4031
NgoMIV G'CCGG_C
Cuts at: 159 557 2549 159
Size: 398 1992 1980
Fragments arranged by size:
1992 1980 398
NotI GC'GGCC_GC
Cuts at: 338 642 338
Size: 304 4066
NspI r_CATG'y
Cuts at: 1423 1923 2830 1423
Size: 500 907 2963
Fragments arranged by size:
2963 907 500
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PmlI CAC'GTG
Cuts at: 584 584
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 873 922 969 1146 873
Size: 49 47 177 4097
Fragments arranged by size:
4097 177 49 47
PstI C_TGCA'G
Cuts at: 305 767 1417 2102 305
Size: 462 650 685 2573
Fragments arranged by size:
2573 685 650 462
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 564 1795 2155 564
Size: 1231 360 2779
Fragments arranged by size:
2779 1231 360
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SacII CC_GC'GG
Cuts at: 128 167 599 759 1246 128
Size: 39 432 160 487 3252
Fragments arranged by size:
3252 487 432 160 39
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SanDI GG'GwC_CC
Cuts at: 922 922
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 382 2393 2603 382
Size: 2011 210 2149
Fragments arranged by size:
2149 2011 210
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 301 763 1413 2098 3091 3282 33
Size: 268 462 650 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 462 268 191
SfoI GGC'GCC
Cuts at: 579 611 1802 2050 579
Size: 32 1191 248 2899
Fragments arranged by size:
2899 1191 248 32
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 919 1392 919
Size: 473 3897
SmlI C'TyrA_G
Cuts at: 1525 2440 2932 3194 1525
Size: 915 492 262 2701
Fragments arranged by size:
2701 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 107 446 1504 1858 107
Size: 339 1058 354 2619
Fragments arranged by size:
2619 1058 354 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 3885 3885
Size: 4370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1092 1124 3887 3919 1092
Size: 32 2763 32 1543
Fragments arranged by size:
2763 1543 32 32
Tth111I GACn'n_nGTC
Cuts at: 1051 2167 1051
Size: 1116 3254
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 973 1865 973
Size: 892 3478
XmaI C'CCGG_G
Cuts at: 917 1390 917
Size: 473 3897
ZraI GAC'GTC
Cuts at: 453 453
Size: 4370
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI