(Circular) (Six-base) MAPSORT of: pF1KE2259.seq Check: 7202 from: 1 to: 4619 pF1KE2259 4619 bp With 182 enzymes: SgfI * October 2, 2018 18:08 .. AccI GT'mk_AC Cuts at: 266 1657 266 Size: 1391 3228 Acc65I G'GTAC_C Cuts at: 975 1635 975 Size: 660 3959 AclI AA'CG_TT Cuts at: 4459 4459 Size: 4619 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 625 2444 2876 3623 3891 625 Size: 1819 432 747 268 1353 Fragments arranged by size: 1819 1353 747 432 268 AfeI AGC'GCT Cuts at: 469 469 Size: 4619 AflIII A'CryG_T Cuts at: 161 494 1971 3075 4052 161 Size: 333 1477 1104 977 728 Fragments arranged by size: 1477 1104 977 728 333 AgeI A'CCGG_T Cuts at: 3007 3007 Size: 4619 AleI CACnn'nnGTG Cuts at: 493 493 Size: 4619 AlwNI CAG_nnn'CTG Cuts at: 528 1924 3491 528 Size: 1396 1567 1656 Fragments arranged by size: 1656 1567 1396 ApaLI G'TGCA_C Cuts at: 1291 3389 1291 Size: 2098 2521 ApoI r'AATT_y Cuts at: 504 1623 3972 4228 504 Size: 1119 2349 256 895 Fragments arranged by size: 2349 1119 895 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4619 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4619 AvaI C'yCGr_G Cuts at: 1150 1387 1639 1150 Size: 237 252 4130 Fragments arranged by size: 4130 252 237 BamHI G'GATC_C Cuts at: 1644 1644 Size: 4619 BanI G'GyrC_C Cuts at: 975 1230 1635 2049 2297 2332 975 Size: 255 405 414 248 35 3262 Fragments arranged by size: 3262 414 405 255 248 35 BanII G_rGCy'C Cuts at: 755 1633 2663 755 Size: 878 1030 2711 Fragments arranged by size: 2711 1030 878 BbeI G_GCGC'C Cuts at: 1234 2053 2301 1234 Size: 819 248 3552 Fragments arranged by size: 3552 819 248 BbsI GAAGACnn'nnnn_ Cuts at: 406 921 1530 406 Size: 515 609 3495 Fragments arranged by size: 3495 609 515 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4585 BciVI GTATCCnnnnn_n' Cuts at: 2517 3278 4619 2517 Size: 761 1341 2517 Fragments arranged by size: 2517 1341 761 BclI T'GATC_A Cuts at: 1010 2139 1010 Size: 1129 3490 BglI GCCn_nnn'nGGC Cuts at: 1286 1286 Size: 4619 BglII A'GATC_T Cuts at: 2134 4171 2134 Size: 2037 2582 BlpI GC'TnA_GC Cuts at: 1731 1731 Size: 4619 Bme1580I G_kGCm'C Cuts at: 1295 2244 2337 3393 1295 Size: 949 93 1056 2521 Fragments arranged by size: 2521 1056 949 93 BmgBI CAC'GTC Cuts at: 300 300 Size: 4619 BmrI ACTGGGnnnn_n' Cuts at: 984 1831 1912 2247 4391 984 Size: 847 81 335 2144 1212 Fragments arranged by size: 2144 1212 847 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2986 2986 Size: 4619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 518 1438 518 Size: 920 3699 Bpu10I CC'TnA_GC Cuts at: 277 277 Size: 4619 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1795 2674 3166 3464 1795 Size: 879 492 298 2950 Fragments arranged by size: 2950 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4323 4323 Size: 4619 BsaAI yAC'GTr Cuts at: 162 1972 162 Size: 1810 2809 BsaBI GATnn'nnATC Cuts at: 6 2133 4170 6 Size: 2127 2037 455 Fragments arranged by size: 2127 2037 455 BsaHI Gr'CG_yC Cuts at: 128 476 1231 2050 2298 3000 4496 128 Size: 348 755 819 248 702 1496 251 Fragments arranged by size: 1496 819 755 702 348 251 248 BsaWI w'CCGG_w Cuts at: 1808 2329 3007 3281 3428 3839 1808 Size: 521 678 274 147 411 2588 Fragments arranged by size: 2588 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1582 1612 1582 Size: 30 4589 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 735 1435 735 Size: 700 3919 BseYI C'CCAG_C Cuts at: 2044 2802 3379 3875 2044 Size: 758 577 496 2788 Fragments arranged by size: 2788 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 142 610 1354 1536 142 Size: 468 744 182 3225 Fragments arranged by size: 3225 744 468 182 BsiEI CG_ry'CG Cuts at: 104 2161 2207 3415 104 Size: 2057 46 1208 1308 Fragments arranged by size: 2057 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 836 1295 1364 1633 2411 2601 3393 836 Size: 459 69 269 778 190 792 2062 Fragments arranged by size: 2062 792 778 459 269 190 69 Bsp1286I G_dGCh'C Cuts at: 755 836 1295 1364 1633 2244 2337 2411 2601 Size: 81 459 69 269 611 93 74 190 Cuts at: 2601 2663 3393 755 Size: 62 730 1981 Fragments arranged by size: 1981 730 611 459 269 190 93 81 74 69 62 BspEI T'CCGG_A Cuts at: 3839 3839 Size: 4619 BspHI T'CATG_A Cuts at: 3795 3795 Size: 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 536 824 1265 1669 1883 2185 2566 536 Size: 288 441 404 214 302 381 2589 Fragments arranged by size: 2589 441 404 381 302 288 214 BsrBI CCG'CTC Cuts at: 50 2911 2965 4449 4614 50 Size: 2861 54 1484 165 55 Fragments arranged by size: 2861 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 516 1433 2531 516 Size: 917 1098 2604 Fragments arranged by size: 2604 1098 917 BsrFI r'CCGG_y Cuts at: 2617 2798 3007 2617 Size: 181 209 4229 Fragments arranged by size: 4229 209 181 BsrGI T'GTAC_A Cuts at: 1262 1262 Size: 4619 BssSI C'ACGA_G Cuts at: 940 2890 3248 940 Size: 1950 358 2311 Fragments arranged by size: 2311 1950 358 BstAPI GCAn_nnn'nTGC Cuts at: 1340 1368 1996 1340 Size: 28 628 3963 Fragments arranged by size: 3963 628 28 BstBI TT'CG_AA Cuts at: 2980 2980 Size: 4619 BstXI CCAn_nnnn'nTGG Cuts at: 1416 1587 1416 Size: 171 4448 BstYI r'GATC_y Cuts at: 1 642 1412 1644 2111 2134 2469 2715 3727 Size: 641 770 232 467 23 335 246 1012 Cuts at: 3727 3813 3825 4171 1 Size: 86 12 346 449 Fragments arranged by size: 1012 770 641 467 449 346 335 246 232 86 23 12 BtgI C'CryG_G Cuts at: 236 1226 1275 236 Size: 990 49 3580 Fragments arranged by size: 3580 990 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2569 2750 2913 2569 Size: 181 163 4275 Fragments arranged by size: 4275 181 163 BtsI GCAGTG_nn' Cuts at: 594 1862 594 Size: 1268 3351 ClaI AT'CG_AT Cuts at: 563 563 Size: 4619 DraI TTT'AAA Cuts at: 1618 1618 Size: 4619 DrdI GACnn_nn'nnGTC Cuts at: 2325 3183 2325 Size: 858 3761 EaeI y'GGCC_r Cuts at: 143 239 420 1205 1329 1406 2204 2378 2769 Size: 96 181 785 124 77 798 174 391 Cuts at: 2769 2796 4141 143 Size: 27 1345 621 Fragments arranged by size: 1345 798 785 621 391 181 174 124 96 77 27 EagI C'GGCC_G Cuts at: 2204 2204 Size: 4619 EarI CTCTTCn'nnn_ Cuts at: 1138 2642 2852 1138 Size: 1504 210 2905 Fragments arranged by size: 2905 1504 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 831 2827 3137 3283 4429 831 Size: 1996 310 146 1146 1021 Fragments arranged by size: 1996 1146 1021 310 146 EcoICRI GAG'CTC Cuts at: 1631 1631 Size: 4619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 518 625 1438 2444 2876 3623 3891 518 Size: 107 813 1006 432 747 268 1246 Fragments arranged by size: 1246 1006 813 747 432 268 107 EcoNI CCTnn'n_nnAGG Cuts at: 228 1420 228 Size: 1192 3427 EcoO109I rG'GnC_Cy Cuts at: 614 696 1395 1758 614 Size: 82 699 363 3475 Fragments arranged by size: 3475 699 363 82 EcoRI G'AATT_C Cuts at: 504 1623 504 Size: 1119 3500 EcoRV GAT'ATC Cuts at: 666 666 Size: 4619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 4619 FspI TGC'GCA Cuts at: 2400 2400 Size: 4619 HaeII r_GCGC'y Cuts at: 142 471 1197 1226 1234 2053 2301 3323 4302 Size: 329 726 29 8 819 248 1022 979 Cuts at: 4302 142 Size: 459 Fragments arranged by size: 1022 979 819 726 459 329 248 29 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1560 1582 1592 1614 2461 2491 2493 2523 1560 Size: 22 10 22 847 30 2 30 3656 Fragments arranged by size: 3656 847 30 30 22 22 10 2 HincII GTy'rAC Cuts at: 1658 1658 Size: 4619 HindIII A'AGCT_T Cuts at: 190 190 Size: 4619 KasI G'GCGC_C Cuts at: 1230 2049 2297 1230 Size: 819 248 3552 Fragments arranged by size: 3552 819 248 KpnI G_GTAC'C Cuts at: 979 1639 979 Size: 660 3959 MluI A'CGCG_T Cuts at: 4052 4052 Size: 4619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3290 3474 3290 Size: 184 4435 MscI TGG'CCA Cuts at: 241 422 1207 1408 2380 241 Size: 181 785 201 972 2480 Fragments arranged by size: 2480 972 785 201 181 MslI CAynn'nnrTG Cuts at: 298 493 1567 2608 2735 298 Size: 195 1074 1041 127 2182 Fragments arranged by size: 2182 1074 1041 195 127 MspA1I CmG'CkG Cuts at: 1191 1277 1451 1730 2044 2404 3417 3662 1191 Size: 86 174 279 314 360 1013 245 2148 Fragments arranged by size: 2148 1013 360 314 279 245 174 86 NaeI GCC'GGC Cuts at: 2800 2800 Size: 4619 NarI GG'CG_CC Cuts at: 1231 2050 2298 1231 Size: 819 248 3552 Fragments arranged by size: 3552 819 248 NgoMIV G'CCGG_C Cuts at: 2798 2798 Size: 4619 NspI r_CATG'y Cuts at: 1672 2172 3079 1672 Size: 500 907 3212 Fragments arranged by size: 3212 907 500 PasI CC'CwG_GG Cuts at: 897 897 Size: 4619 PciI A'CATG_T Cuts at: 3075 3075 Size: 4619 PflMI CCAn_nnn'nTGG Cuts at: 335 335 Size: 4619 PfoI T'CCnGG_A Cuts at: 1415 1415 Size: 4619 PmeI GTTT'AAAC Cuts at: 1618 1618 Size: 4619 PmlI CAC'GTG Cuts at: 162 162 Size: 4619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1474 1506 3758 3790 1474 Size: 32 2252 32 2303 Fragments arranged by size: 2303 2252 32 32 PpuMI rG'GwC_Cy Cuts at: 614 696 1395 614 Size: 82 699 3838 Fragments arranged by size: 3838 699 82 PshAI GACnn'nnGTC Cuts at: 1102 1102 Size: 4619 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1246 1278 1246 Size: 32 4587 PstI C_TGCA'G Cuts at: 290 650 876 1055 1201 1480 1666 2351 290 Size: 360 226 179 146 279 186 685 2558 Fragments arranged by size: 2558 685 360 279 226 186 179 146 PvuI CG_AT'CG Cuts at: 104 104 Size: 4619 PvuII CAG'CTG Cuts at: 2044 2404 2044 Size: 360 4259 RsrII CG'GwC_CG Cuts at: 2814 2814 Size: 4619 SacI G_AGCT'C Cuts at: 1633 1633 Size: 4619 SacII CC_GC'GG Cuts at: 1278 1278 Size: 4619 SalI G'TCGA_C Cuts at: 1656 1656 Size: 4619 SanDI GG'GwC_CC Cuts at: 1395 1395 Size: 4619 SapI GCTCTTCn'nnn_ Cuts at: 2642 2852 2642 Size: 210 4409 SbfI CC_TGCA'GG Cuts at: 1666 1666 Size: 4619 ScaI AGT'ACT Cuts at: 994 4136 994 Size: 3142 1477 SfcI C'TryA_G Cuts at: 33 286 646 872 1051 1197 1476 1662 2347 Size: 253 360 226 179 146 279 186 685 Cuts at: 2347 3340 3531 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 685 360 279 253 226 191 186 179 146 SfoI GGC'GCC Cuts at: 1232 2051 2299 1232 Size: 819 248 3552 Fragments arranged by size: 3552 819 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4619 SmaI CCC'GGG Cuts at: 1152 1641 1152 Size: 489 4130 SmlI C'TyrA_G Cuts at: 1774 2689 3181 3443 1774 Size: 915 492 262 2950 Fragments arranged by size: 2950 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4619 SphI G_CATG'C Cuts at: 1672 2172 1672 Size: 500 4119 StyI C'CwwG_G Cuts at: 224 638 1753 2107 224 Size: 414 1115 354 2736 Fragments arranged by size: 2736 1115 414 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1524 2202 2335 2794 3003 1524 Size: 678 133 459 209 3140 Fragments arranged by size: 3140 678 459 209 133 TatI w'GTAC_w Cuts at: 992 1262 4134 992 Size: 270 2872 1477 Fragments arranged by size: 2872 1477 270 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1501 1533 4136 4168 1501 Size: 32 2603 32 1952 Fragments arranged by size: 2603 1952 32 32 Tth111I GACn'n_nGTC Cuts at: 2416 2416 Size: 4619 XbaI T'CTAG_A Cuts at: 1650 1650 Size: 4619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1014 2114 1014 Size: 1100 3519 XmaI C'CCGG_G Cuts at: 1150 1639 1150 Size: 489 4130 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I CspCI DraIII FseI FspAI HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PsiI PspOMI PspXI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI