Restriction map for
pF1KE2311
(Circular) (Six-base) MAPSORT of: pF1KE2311.seq Check: 12 from: 1 to: 8576
pF1KE2311 8576 bp
With 182 enzymes: SgfI *
January 5, 2011 13:33 ..
AccI GT'mk_AC
Cuts at: 5614 5614
Size: 8576
Acc65I G'GTAC_C
Cuts at: 3508 3577 5592 3508
Size: 69 2015 6492
Fragments arranged by size:
6492 2015 69
AclI AA'CG_TT
Cuts at: 8416 8416
Size: 8576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 2546 3306 3518 4664 5477 6401 6833 7580
Size: 1666 760 212 1146 813 924 432 747
Cuts at: 7580 7848 880
Size: 268 1608
Fragments arranged by size:
1666 1608 1146 924 813 760 747 432
268 212
AflII C'TTAA_G
Cuts at: 768 768
Size: 8576
AflIII A'CryG_T
Cuts at: 5928 7032 8009 5928
Size: 1104 977 6495
Fragments arranged by size:
6495 1104 977
AgeI A'CCGG_T
Cuts at: 6964 6964
Size: 8576
AleI CACnn'nnGTG
Cuts at: 939 3703 939
Size: 2764 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4345 4345
Size: 8576
AlwNI CAG_nnn'CTG
Cuts at: 210 294 5881 7448 210
Size: 84 5587 1567 1338
Fragments arranged by size:
5587 1567 1338 84
ApaLI G'TGCA_C
Cuts at: 878 7346 878
Size: 6468 2108
ApoI r'AATT_y
Cuts at: 374 641 1045 1461 3856 4746 5580 7929 8185
Size: 267 404 416 2395 890 834 2349 256
Cuts at: 8185 374
Size: 765
Fragments arranged by size:
2395 2349 890 834 765 416 404 267
256
AseI AT'TA_AT
Cuts at: 20 2690 20
Size: 2670 5906
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8576
AvaI C'yCGr_G
Cuts at: 2284 5596 2284
Size: 3312 5264
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3373 3406 3500 3533 3373
Size: 33 94 33 8416
Fragments arranged by size:
8416 94 33 33
BamHI G'GATC_C
Cuts at: 5601 5601
Size: 8576
BanI G'GyrC_C
Cuts at: 3508 3577 5592 6006 6254 6289 3508
Size: 69 2015 414 248 35 5795
Fragments arranged by size:
5795 2015 414 248 69 35
BanII G_rGCy'C
Cuts at: 152 4047 5590 6620 152
Size: 3895 1543 1030 2108
Fragments arranged by size:
3895 2108 1543 1030
BbeI G_GCGC'C
Cuts at: 6010 6258 6010
Size: 248 8328
BbsI GAAGACnn'nnnn_
Cuts at: 426 507 859 5275 5492 426
Size: 81 352 4416 217 3510
Fragments arranged by size:
4416 3510 352 217 81
BbvCI CC'TCA_GC
Cuts at: 3651 3651
Size: 8576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2563 2597 2563
Size: 34 8542
BciVI GTATCCnnnnn_n'
Cuts at: 543 1228 6474 7235 8576 543
Size: 685 5246 761 1341 543
Fragments arranged by size:
5246 1341 761 685 543
BclI T'GATC_A
Cuts at: 324 6096 324
Size: 5772 2804
BfrBI ATG'CAT
Cuts at: 633 4714 633
Size: 4081 4495
BglII A'GATC_T
Cuts at: 2503 4143 5289 6091 8128 2503
Size: 1640 1146 802 2037 2951
Fragments arranged by size:
2951 2037 1640 1146 802
BlpI GC'TnA_GC
Cuts at: 5688 5688
Size: 8576
Bme1580I G_kGCm'C
Cuts at: 882 6201 6294 7350 882
Size: 5319 93 1056 2108
Fragments arranged by size:
5319 2108 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 983 4041 5788 5869 6204 8348 983
Size: 3058 1747 81 335 2144 1211
Fragments arranged by size:
3058 2144 1747 1211 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5469 6943 5469
Size: 1474 7102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 1088 2637 413
Size: 675 1549 6352
Fragments arranged by size:
6352 1549 675
Bpu10I CC'TnA_GC
Cuts at: 203 3651 203
Size: 3448 5128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 3288 3406 5752 6631 7123 7421 610
Size: 2678 118 2346 879 492 298 1765
Fragments arranged by size:
2678 2346 1765 879 492 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 8280 8280
Size: 8576
BsaAI yAC'GTr
Cuts at: 1276 4036 5929 1276
Size: 2760 1893 3923
Fragments arranged by size:
3923 2760 1893
BsaBI GATnn'nnATC
Cuts at: 6 963 5288 6090 8127 6
Size: 957 4325 802 2037 455
Fragments arranged by size:
4325 2037 957 802 455
BsaHI Gr'CG_yC
Cuts at: 4897 6007 6255 6957 8453 4897
Size: 1110 248 702 1496 5020
Fragments arranged by size:
5020 1496 1110 702 248
BsaWI w'CCGG_w
Cuts at: 5765 6286 6964 7238 7385 7796 5765
Size: 521 678 274 147 411 6545
Fragments arranged by size:
6545 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1616 1963 5476 1616
Size: 347 3513 4716
Fragments arranged by size:
4716 3513 347
BseYI C'CCAG_C
Cuts at: 538 3425 4222 4508 6001 6759 7336 7832 538
Size: 2887 797 286 1493 758 577 496 1282
Fragments arranged by size:
2887 1493 1282 797 758 577 496 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 3558 963
Size: 2595 5981
BsiEI CG_ry'CG
Cuts at: 104 145 6118 6164 7372 104
Size: 41 5973 46 1208 1308
Fragments arranged by size:
5973 1308 1208 46 41
BsiHKAI G_wGCw'C
Cuts at: 152 882 5590 6368 6558 7350 152
Size: 730 4708 778 190 792 1378
Fragments arranged by size:
4708 1378 792 778 730 190
BsmI GAATG_Cn'
Cuts at: 4537 4537
Size: 8576
BsmBI CGTCTCn'nnnn_
Cuts at: 2070 2070
Size: 8576
Bsp1286I G_dGCh'C
Cuts at: 152 882 4047 5590 6201 6294 6368 6558 6620
Size: 730 3165 1543 611 93 74 190 62
Cuts at: 6620 7350 152
Size: 730 1378
Fragments arranged by size:
3165 1543 1378 730 730 611 190 93
74 62
BspEI T'CCGG_A
Cuts at: 7796 7796
Size: 8576
BspHI T'CATG_A
Cuts at: 1500 1566 2436 7752 1500
Size: 66 870 5316 2324
Fragments arranged by size:
5316 2324 870 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1047 2083 5626 5840 6142 6523 1047
Size: 1036 3543 214 302 381 3100
Fragments arranged by size:
3543 3100 1036 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2247 6868 6922 8406 8571 50
Size: 2197 4621 54 1484 165 55
Fragments arranged by size:
4621 2197 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 542 6488 542
Size: 5946 2630
BsrFI r'CCGG_y
Cuts at: 4137 6574 6755 6964 4137
Size: 2437 181 209 5749
Fragments arranged by size:
5749 2437 209 181
BssSI C'ACGA_G
Cuts at: 6847 7205 6847
Size: 358 8218
BstAPI GCAn_nnn'nTGC
Cuts at: 5953 5953
Size: 8576
BstBI TT'CG_AA
Cuts at: 870 4620 5483 6937 870
Size: 3750 863 1454 2509
Fragments arranged by size:
3750 2509 1454 863
BstEII G'GTnAC_C
Cuts at: 1891 3313 1891
Size: 1422 7154
BstXI CCAn_nnnn'nTGG
Cuts at: 1648 2939 1648
Size: 1291 7285
BstYI r'GATC_y
Cuts at: 1 472 1590 2250 2451 2503 3186 3672 3720
Size: 471 1118 660 201 52 683 486 48
Cuts at: 3720 4143 4496 4647 5289 5601 6068 6091 6426
Size: 423 353 151 642 312 467 23 335
Cuts at: 6426 6672 7684 7770 7782 8128 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1118 1012 683 660 642 486 471 467
449 423 353 346 335 312 246 201
151 86 52 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 1983 1983
Size: 8576
BtgI C'CryG_G
Cuts at: 3082 3668 4986 3082
Size: 586 1318 6672
Fragments arranged by size:
6672 1318 586
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 147 3315 6526 6707 6870 147
Size: 3168 3211 181 163 1853
Fragments arranged by size:
3211 3168 1853 181 163
BtsI GCAGTG_nn'
Cuts at: 617 951 5819 617
Size: 334 4868 3374
Fragments arranged by size:
4868 3374 334
DraI TTT'AAA
Cuts at: 1290 5575 1290
Size: 4285 4291
DrdI GACnn_nn'nnGTC
Cuts at: 2361 6282 7140 2361
Size: 3921 858 3797
Fragments arranged by size:
3921 3797 858
EaeI y'GGCC_r
Cuts at: 224 1795 1929 4983 6161 6335 6726 6753 8098
Size: 1571 134 3054 1178 174 391 27 1345
Cuts at: 8098 224
Size: 702
Fragments arranged by size:
3054 1571 1345 1178 702 391 174 134
27
EagI C'GGCC_G
Cuts at: 6161 6161
Size: 8576
EarI CTCTTCn'nnn_
Cuts at: 365 1290 1300 2363 2510 2531 2606 3383 3959
Size: 925 10 1063 147 21 75 777 576
Cuts at: 3959 4397 5008 5363 5453 6599 6809 365
Size: 438 611 355 90 1146 210 2132
Fragments arranged by size:
2132 1146 1063 925 777 611 576 438
355 210 147 90 75 21 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3227 6784 7094 7240 8386 3227
Size: 3557 310 146 1146 3417
Fragments arranged by size:
3557 3417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 150 5588 150
Size: 5438 3138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 880 1088 2546 2637 3306 3518 4664 5477
Size: 467 208 1458 91 669 212 1146 813
Cuts at: 5477 6401 6833 7580 7848 413
Size: 924 432 747 268 1141
Fragments arranged by size:
1458 1146 1141 924 813 747 669 467
432 268 212 208 91
EcoO109I rG'GnC_Cy
Cuts at: 281 355 5020 5715 281
Size: 74 4665 695 3142
Fragments arranged by size:
4665 3142 695 74
EcoRI G'AATT_C
Cuts at: 641 1461 4746 5580 641
Size: 820 3285 834 3637
Fragments arranged by size:
3637 3285 834 820
EcoRV GAT'ATC
Cuts at: 961 1912 961
Size: 951 7625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 443 5450 6595 443
Size: 5007 1145 2424
Fragments arranged by size:
5007 2424 1145
FspI TGC'GCA
Cuts at: 6357 6357
Size: 8576
HaeII r_GCGC'y
Cuts at: 1059 6010 6258 7280 8259 1059
Size: 4951 248 1022 979 1376
Fragments arranged by size:
4951 1376 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 134 159 166 1165 1197 1752 1784 3526
Size: 7 25 7 999 32 555 32 1742
Cuts at: 3526 3533 3558 3565 3634 3666 4369 4401 4654
Size: 7 25 7 69 32 703 32 253
Cuts at: 4654 4686 5334 5366 5510 5542 6418 6448 6450
Size: 32 648 32 144 32 876 30 2
Cuts at: 6450 6480 127
Size: 30 2223
Fragments arranged by size:
2223 1742 999 876 703 648 555 253
144 69 32 32 32 32 32 32
32 30 30 25 25 7 7 7
7 2
HincII GTy'rAC
Cuts at: 522 1212 1477 5537 5615 522
Size: 690 265 4060 78 3483
Fragments arranged by size:
4060 3483 690 265 78
HindIII A'AGCT_T
Cuts at: 4766 4766
Size: 8576
HpaI GTT'AAC
Cuts at: 522 522
Size: 8576
KasI G'GCGC_C
Cuts at: 6006 6254 6006
Size: 248 8328
KpnI G_GTAC'C
Cuts at: 3512 3581 5596 3512
Size: 69 2015 6492
Fragments arranged by size:
6492 2015 69
MluI A'CGCG_T
Cuts at: 8009 8009
Size: 8576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 628 1253 1298 3033 3739 7247 7431 628
Size: 625 45 1735 706 3508 184 1773
Fragments arranged by size:
3508 1773 1735 706 625 184 45
MscI TGG'CCA
Cuts at: 4985 6337 4985
Size: 1352 7224
MslI CAynn'nnrTG
Cuts at: 628 939 1378 1505 2435 2630 2937 3703 4186
Size: 311 439 127 930 195 307 766 483
Cuts at: 4186 6565 6692 628
Size: 2379 127 2512
Fragments arranged by size:
2512 2379 930 766 483 439 311 307
195 127 127
MspA1I CmG'CkG
Cuts at: 832 2296 4684 4921 5475 5687 6001 6361 7374
Size: 1464 2388 237 554 212 314 360 1013
Cuts at: 7374 7619 832
Size: 245 1789
Fragments arranged by size:
2388 1789 1464 1013 554 360 314 245
237 212
NaeI GCC'GGC
Cuts at: 6757 6757
Size: 8576
NarI GG'CG_CC
Cuts at: 6007 6255 6007
Size: 248 8328
NcoI C'CATG_G
Cuts at: 3082 4986 3082
Size: 1904 6672
NgoMIV G'CCGG_C
Cuts at: 6755 6755
Size: 8576
NsiI A_TGCA'T
Cuts at: 635 4716 635
Size: 4081 4495
NspI r_CATG'y
Cuts at: 460 781 1700 5183 5629 6129 7036 460
Size: 321 919 3483 446 500 907 2000
Fragments arranged by size:
3483 2000 919 907 500 446 321
PasI CC'CwG_GG
Cuts at: 3587 3587
Size: 8576
PciI A'CATG_T
Cuts at: 7032 7032
Size: 8576
PflMI CCAn_nnn'nTGG
Cuts at: 294 294
Size: 8576
PfoI T'CCnGG_A
Cuts at: 173 390 2447 173
Size: 217 2057 6302
Fragments arranged by size:
6302 2057 217
PmeI GTTT'AAAC
Cuts at: 5575 5575
Size: 8576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3981 4013 7715 7747 3981
Size: 32 3702 32 4810
Fragments arranged by size:
4810 3702 32 32
PpuMI rG'GwC_Cy
Cuts at: 281 355 5020 281
Size: 74 4665 3837
Fragments arranged by size:
4665 3837 74
PshAI GACnn'nnGTC
Cuts at: 654 654
Size: 8576
PsiI TTA'TAA
Cuts at: 1426 3886 3905 3923 4382 1426
Size: 2460 19 18 459 5620
Fragments arranged by size:
5620 2460 459 19 18
PstI C_TGCA'G
Cuts at: 612 1434 2699 5623 6308 612
Size: 822 1265 2924 685 2880
Fragments arranged by size:
2924 2880 1265 822 685
PvuI CG_AT'CG
Cuts at: 104 145 104
Size: 41 8535
PvuII CAG'CTG
Cuts at: 832 2296 4684 4921 5475 6001 6361 832
Size: 1464 2388 237 554 526 360 3047
Fragments arranged by size:
3047 2388 1464 554 526 360 237
RsrII CG'GwC_CG
Cuts at: 6771 6771
Size: 8576
SacI G_AGCT'C
Cuts at: 152 5590 152
Size: 5438 3138
SalI G'TCGA_C
Cuts at: 5613 5613
Size: 8576
SapI GCTCTTCn'nnn_
Cuts at: 1300 4397 6599 6809 1300
Size: 3097 2202 210 3067
Fragments arranged by size:
3097 3067 2202 210
SbfI CC_TGCA'GG
Cuts at: 5623 5623
Size: 8576
ScaI AGT'ACT
Cuts at: 5165 8093 5165
Size: 2928 5648
SfcI C'TryA_G
Cuts at: 33 608 925 1430 2695 4164 5104 5354 5619
Size: 575 317 505 1265 1469 940 250 265
Cuts at: 5619 6304 7297 7488 33
Size: 685 993 191 1121
Fragments arranged by size:
1469 1265 1121 993 940 685 575 505
317 265 250 191
SfoI GGC'GCC
Cuts at: 6008 6256 6008
Size: 248 8328
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8576
SmaI CCC'GGG
Cuts at: 5598 5598
Size: 8576
SmlI C'TyrA_G
Cuts at: 589 768 3267 3385 5731 6646 7138 7400 589
Size: 179 2499 118 2346 915 492 262 1765
Fragments arranged by size:
2499 2346 1765 915 492 262 179 118
SnaBI TAC'GTA
Cuts at: 4036 4036
Size: 8576
SpeI A'CTAG_T
Cuts at: 67 1234 67
Size: 1167 7409
SphI G_CATG'C
Cuts at: 1700 5629 6129 1700
Size: 3929 500 4147
Fragments arranged by size:
4147 3929 500
StuI AGG'CCT
Cuts at: 128 128
Size: 8576
StyI C'CwwG_G
Cuts at: 1641 3082 3457 4986 5710 6064 1641
Size: 1441 375 1529 724 354 4153
Fragments arranged by size:
4153 1529 1441 724 375 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 552 2977 6159 6292 6751 6960 552
Size: 2425 3182 133 459 209 2168
Fragments arranged by size:
3182 2425 2168 459 209 133
TatI w'GTAC_w
Cuts at: 396 665 3727 4884 5163 8091 396
Size: 269 3062 1157 279 2928 881
Fragments arranged by size:
3062 2928 1157 881 279 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3297 3329 8093 8125 3297
Size: 32 4764 32 3748
Fragments arranged by size:
4764 3748 32 32
Tth111I GACn'n_nGTC
Cuts at: 188 6373 188
Size: 6185 2391
XbaI T'CTAG_A
Cuts at: 3183 5607 3183
Size: 2424 6152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6071 6071
Size: 8576
XmaI C'CCGG_G
Cuts at: 5596 5596
Size: 8576
XmnI GAAnn'nnTTC
Cuts at: 3377 5165 3377
Size: 1788 6788
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BglI BmgBI BmtI BsaXI BsiWI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI MfeI NdeI
NheI NotI NruI PacI PmlI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI ZraI