Restriction map for
pF1KE2321
(Circular) (Six-base) MAPSORT of: pF1KE2321.seq Check: 674 from: 1 to: 7415
pF1KE2321 7415 bp
With 182 enzymes: SgfI *
January 27, 2011 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 798 3836 798
Size: 3038 4377
AatII G_ACGT'C
Cuts at: 1166 1987 4178 1166
Size: 821 2191 4403
Fragments arranged by size:
4403 2191 821
AccI GT'mk_AC
Cuts at: 4453 4453
Size: 7415
Acc65I G'GTAC_C
Cuts at: 4431 4431
Size: 7415
AclI AA'CG_TT
Cuts at: 7255 7255
Size: 7415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 1178 1678 1819 1857 2878 4020 4159 5240
Size: 750 500 141 38 1021 1142 139 1081
Cuts at: 5240 5672 6419 6687 428
Size: 432 747 268 1156
Fragments arranged by size:
1156 1142 1081 1021 750 747 500 432
268 141 139 38
AflII C'TTAA_G
Cuts at: 1075 1075
Size: 7415
AflIII A'CryG_T
Cuts at: 119 3668 4767 5871 6848 119
Size: 3549 1099 1104 977 686
Fragments arranged by size:
3549 1104 1099 977 686
AgeI A'CCGG_T
Cuts at: 5803 5803
Size: 7415
AhdI GACnn_n'nnGTC
Cuts at: 846 846
Size: 7415
AleI CACnn'nnGTG
Cuts at: 2188 3040 3221 3559 2188
Size: 852 181 338 6044
Fragments arranged by size:
6044 852 338 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2444 3473 3532 2444
Size: 1029 59 6327
Fragments arranged by size:
6327 1029 59
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 106 138 106
Size: 32 7383
AlwNI CAG_nnn'CTG
Cuts at: 483 546 1865 1880 2010 2332 2909 3195 3356
Size: 63 1319 15 130 322 577 286 161
Cuts at: 3356 3495 4720 6287 483
Size: 139 1225 1567 1611
Fragments arranged by size:
1611 1567 1319 1225 577 322 286 161
139 130 63 15
ApaI G_GGCC'C
Cuts at: 406 2708 406
Size: 2302 5113
ApaLI G'TGCA_C
Cuts at: 2503 3685 6185 2503
Size: 1182 2500 3733
Fragments arranged by size:
3733 2500 1182
ApoI r'AATT_y
Cuts at: 2581 4419 6768 7024 2581
Size: 1838 2349 256 2972
Fragments arranged by size:
2972 2349 1838 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7415
AvaI C'yCGr_G
Cuts at: 564 571 3719 4061 4435 564
Size: 7 3148 342 374 3544
Fragments arranged by size:
3544 3148 374 342 7
BamHI G'GATC_C
Cuts at: 1376 2852 4440 1376
Size: 1476 1588 4351
Fragments arranged by size:
4351 1588 1476
BanI G'GyrC_C
Cuts at: 256 786 1763 2275 2609 3871 3968 4431 4845
Size: 530 977 512 334 1262 97 463 414
Cuts at: 4845 5093 5128 256
Size: 248 35 2543
Fragments arranged by size:
2543 1262 977 530 512 463 414 334
248 97 35
BanII G_rGCy'C
Cuts at: 406 1123 1325 1721 2708 2882 3139 3371 4137
Size: 717 202 396 987 174 257 232 766
Cuts at: 4137 4429 5459 406
Size: 292 1030 2362
Fragments arranged by size:
2362 1030 987 766 717 396 292 257
232 202 174
BbeI G_GCGC'C
Cuts at: 260 4849 5097 260
Size: 4589 248 2578
Fragments arranged by size:
4589 2578 248
BbsI GAAGACnn'nnnn_
Cuts at: 882 1166 1987 2916 2937 3219 3954 4026 882
Size: 284 821 929 21 282 735 72 4271
Fragments arranged by size:
4271 929 821 735 284 282 72 21
BbvCI CC'TCA_GC
Cuts at: 584 3505 584
Size: 2921 4494
BciVI GTATCCnnnnn_n'
Cuts at: 3767 5313 6074 7415 3767
Size: 1546 761 1341 3767
Fragments arranged by size:
3767 1546 1341 761
BclI T'GATC_A
Cuts at: 230 1209 4935 230
Size: 979 3726 2710
Fragments arranged by size:
3726 2710 979
BfrBI ATG'CAT
Cuts at: 1131 1637 1131
Size: 506 6909
BglI GCCn_nnn'nGGC
Cuts at: 698 2132 698
Size: 1434 5981
BglII A'GATC_T
Cuts at: 2447 4348 4930 6967 2447
Size: 1901 582 2037 2895
Fragments arranged by size:
2895 2037 1901 582
BlpI GC'TnA_GC
Cuts at: 745 4527 745
Size: 3782 3633
Bme1580I G_kGCm'C
Cuts at: 406 789 1894 2165 2507 2708 3689 5040 5133
Size: 383 1105 271 342 201 981 1351 93
Cuts at: 5133 6189 406
Size: 1056 1632
Fragments arranged by size:
1632 1351 1105 1056 981 383 342 271
201 93
BmrI ACTGGGnnnn_n'
Cuts at: 1047 2157 2848 4627 4708 5043 7187 1047
Size: 1110 691 1779 81 335 2144 1275
Fragments arranged by size:
2144 1779 1275 1110 691 335 81
BmtI G_CTAG'C
Cuts at: 2326 2326
Size: 7415
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 633 709 5782 633
Size: 76 5073 2266
Fragments arranged by size:
5073 2266 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 448 1718 1873 1963 3038 3115 3295 3340
Size: 78 1270 155 90 1075 77 180 45
Cuts at: 3340 3592 370
Size: 252 4193
Fragments arranged by size:
4193 1270 1075 252 180 155 90 78
77 45
Bpu10I CC'TnA_GC
Cuts at: 331 584 1715 3115 3505 331
Size: 253 1131 1400 390 4241
Fragments arranged by size:
4241 1400 1131 390 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1009 1294 4591 5470 5962 6260 709
Size: 300 285 3297 879 492 298 1864
Fragments arranged by size:
3297 1864 879 492 300 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 370 424 4000 7119 370
Size: 54 3576 3119 666
Fragments arranged by size:
3576 3119 666 54
BsaAI yAC'GTr
Cuts at: 384 4089 4768 384
Size: 3705 679 3031
Fragments arranged by size:
3705 3031 679
BsaBI GATnn'nnATC
Cuts at: 6 2023 4929 6966 6
Size: 2017 2906 2037 455
Fragments arranged by size:
2906 2037 2017 455
BsaHI Gr'CG_yC
Cuts at: 257 1163 1984 4175 4250 4846 5094 5796 7292
Size: 906 821 2191 75 596 248 702 1496
Cuts at: 7292 257
Size: 380
Fragments arranged by size:
2191 1496 906 821 702 596 380 248
75
BsaWI w'CCGG_w
Cuts at: 2173 3770 4604 5125 5803 6077 6224 6635 2173
Size: 1597 834 521 678 274 147 411 2953
Fragments arranged by size:
2953 1597 834 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2062 2092 2101 2131 2650 2665 2680 2695 2948
Size: 30 9 30 519 15 15 15 253
Cuts at: 2948 2978 3184 3214 4111 4141 2062
Size: 30 206 30 897 30 5336
Fragments arranged by size:
5336 897 519 253 206 30 30 30
30 30 15 15 15 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2658 3337 3589 3648 3747 3824 2658
Size: 679 252 59 99 77 6249
Fragments arranged by size:
6249 679 252 99 77 59
BseYI C'CCAG_C
Cuts at: 1553 1608 3617 3817 4223 4840 5598 6175 6671
Size: 55 2009 200 406 617 758 577 496
Cuts at: 6671 1553
Size: 2297
Fragments arranged by size:
2297 2009 758 617 577 496 406 200
55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1401 1639 2644 3369 4111 1401
Size: 238 1005 725 742 4705
Fragments arranged by size:
4705 1005 742 725 238
BsiEI CG_ry'CG
Cuts at: 104 315 530 885 4957 5003 6211 104
Size: 211 215 355 4072 46 1208 1308
Fragments arranged by size:
4072 1308 1208 355 215 211 46
BsiHKAI G_wGCw'C
Cuts at: 1084 1325 1721 2507 2882 3371 3689 3816 4429
Size: 241 396 786 375 489 318 127 613
Cuts at: 4429 5207 5397 6189 1084
Size: 778 190 792 2310
Fragments arranged by size:
2310 792 786 778 613 489 396 375
318 241 190 127
BsiWI C'GTAC_G
Cuts at: 4325 4325
Size: 7415
BsmI GAATG_Cn'
Cuts at: 1359 3261 1359
Size: 1902 5513
BsmBI CGTCTCn'nnnn_
Cuts at: 169 2670 3175 169
Size: 2501 505 4409
Fragments arranged by size:
4409 2501 505
Bsp1286I G_dGCh'C
Cuts at: 406 789 1084 1123 1325 1721 1894 2165 2507
Size: 383 295 39 202 396 173 271 342
Cuts at: 2507 2708 2882 3139 3371 3689 3816 4137 4429
Size: 201 174 257 232 318 127 321 292
Cuts at: 4429 5040 5133 5207 5397 5459 6189 406
Size: 611 93 74 190 62 730 1632
Fragments arranged by size:
1632 730 611 396 383 342 321 318
295 292 271 257 232 202 201 190
174 173 127 93 74 62 39
BspEI T'CCGG_A
Cuts at: 2173 6635 2173
Size: 4462 2953
BspHI T'CATG_A
Cuts at: 4217 6591 4217
Size: 2374 5041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 798 1386 3836 4465 4679 4981 5362 798
Size: 588 2450 629 214 302 381 2851
Fragments arranged by size:
2851 2450 629 588 381 302 214
BsrBI CCG'CTC
Cuts at: 50 210 1686 3270 3327 5707 5761 7245 7410
Size: 160 1476 1584 57 2380 54 1484 165
Cuts at: 7410 50
Size: 55
Fragments arranged by size:
2380 1584 1484 1476 165 160 57 55
54
BsrDI GCAATG_nn'
Cuts at: 365 921 1713 2559 3006 3878 4358 5327 365
Size: 556 792 846 447 872 480 969 2453
Fragments arranged by size:
2453 969 872 846 792 556 480 447
BsrFI r'CCGG_y
Cuts at: 314 525 529 2216 3597 3874 3971 4252 5413
Size: 211 4 1687 1381 277 97 281 1161
Cuts at: 5413 5594 5803 314
Size: 181 209 1926
Fragments arranged by size:
1926 1687 1381 1161 281 277 211 209
181 97 4
BsrGI T'GTAC_A
Cuts at: 4046 4046
Size: 7415
BssSI C'ACGA_G
Cuts at: 2780 3241 3250 5686 6044 2780
Size: 461 9 2436 358 4151
Fragments arranged by size:
4151 2436 461 358 9
BstAPI GCAn_nnn'nTGC
Cuts at: 3495 4792 3495
Size: 1297 6118
BstBI TT'CG_AA
Cuts at: 2234 5776 2234
Size: 3542 3873
BstEII G'GTnAC_C
Cuts at: 2535 2535
Size: 7415
BstXI CCAn_nnnn'nTGG
Cuts at: 426 504 639 2355 3561 3957 426
Size: 78 135 1716 1206 396 3884
Fragments arranged by size:
3884 1716 1206 396 135 78
BstYI r'GATC_y
Cuts at: 1 507 1376 2447 2559 2852 4348 4440 4907
Size: 506 869 1071 112 293 1496 92 467
Cuts at: 4907 4930 5265 5511 6523 6609 6621 6967 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1496 1071 1012 869 506 467 449 346
335 293 246 112 92 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 969 1486 969
Size: 517 6898
BtgI C'CryG_G
Cuts at: 107 503 1421 1428 2483 3744 3950 4181 107
Size: 396 918 7 1055 1261 206 231 3341
Fragments arranged by size:
3341 1261 1055 918 396 231 206 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2714 5365 5546 5709 2714
Size: 2651 181 163 4420
Fragments arranged by size:
4420 2651 181 163
BtsI GCAGTG_nn'
Cuts at: 557 4658 557
Size: 4101 3314
DraI TTT'AAA
Cuts at: 4414 4414
Size: 7415
DraIII CAC_nnn'GTG
Cuts at: 2817 2817
Size: 7415
DrdI GACnn_nn'nnGTC
Cuts at: 1540 3621 3790 5121 5979 1540
Size: 2081 169 1331 858 2976
Fragments arranged by size:
2976 2081 1331 858 169
EaeI y'GGCC_r
Cuts at: 312 527 1952 2345 2369 2744 3044 3599 3698
Size: 215 1425 393 24 375 300 555 99
Cuts at: 3698 5000 5174 5565 5592 6937 312
Size: 1302 174 391 27 1345 790
Fragments arranged by size:
1425 1345 1302 790 555 393 391 375
300 215 174 99 27 24
EagI C'GGCC_G
Cuts at: 312 527 5000 312
Size: 215 4473 2727
Fragments arranged by size:
4473 2727 215
EarI CTCTTCn'nnn_
Cuts at: 172 1189 1262 1726 2632 2888 3010 3998 4367
Size: 1017 73 464 906 256 122 988 369
Cuts at: 4367 5438 5648 172
Size: 1071 210 1939
Fragments arranged by size:
1939 1071 1017 988 906 464 369 256
210 122 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1365 2070 3019 3361 4132 5623 5933 6079 7225
Size: 705 949 342 771 1491 310 146 1146
Cuts at: 7225 1365
Size: 1555
Fragments arranged by size:
1555 1491 1146 949 771 705 342 310
146
EcoICRI GAG'CTC
Cuts at: 1323 1719 2880 3369 4427 1323
Size: 396 1161 489 1058 4311
Fragments arranged by size:
4311 1161 1058 489 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 428 448 1178 1678 1718 1819 1857 1873
Size: 58 20 730 500 40 101 38 16
Cuts at: 1873 1963 2878 3038 3115 3295 3340 3592 4020
Size: 90 915 160 77 180 45 252 428
Cuts at: 4020 4159 5240 5672 6419 6687 370
Size: 139 1081 432 747 268 1098
Fragments arranged by size:
1098 1081 915 747 730 500 432 428
268 252 180 160 139 101 90 77
58 45 40 38 20 16
EcoNI CCTnn'n_nnAGG
Cuts at: 280 1693 1996 3508 280
Size: 1413 303 1512 4187
Fragments arranged by size:
4187 1512 1413 303
EcoO109I rG'GnC_Cy
Cuts at: 186 402 403 452 487 560 568 650 663
Size: 216 1 49 35 73 8 82 13
Cuts at: 663 1605 1788 2285 2906 3246 4554 186
Size: 942 183 497 621 340 1308 3047
Fragments arranged by size:
3047 1308 942 621 497 340 216 183
82 73 49 35 13 8 1
EcoRI G'AATT_C
Cuts at: 4419 4419
Size: 7415
EcoRV GAT'ATC
Cuts at: 3921 3921
Size: 7415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1551 1983 2940 4050 5434 1551
Size: 432 957 1110 1384 3532
Fragments arranged by size:
3532 1384 1110 957 432
FseI GG_CCGG'CC
Cuts at: 318 318
Size: 7415
FspI TGC'GCA
Cuts at: 5196 5196
Size: 7415
HaeII r_GCGC'y
Cuts at: 260 347 1865 2011 4849 5097 6119 7098 260
Size: 87 1518 146 2838 248 1022 979 577
Fragments arranged by size:
2838 1518 1022 979 577 248 146 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 832 864 2062 2094 2948 2980 3568
Size: 32 253 32 1198 32 854 32 588
Cuts at: 3568 3600 5257 5287 5289 5319 547
Size: 32 1657 30 2 30 2643
Fragments arranged by size:
2643 1657 1198 854 588 253 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 309 2070 2967 3155 3294 4454 309
Size: 1761 897 188 139 1160 3270
Fragments arranged by size:
3270 1761 1160 897 188 139
HindIII A'AGCT_T
Cuts at: 2423 3894 2423
Size: 1471 5944
KasI G'GCGC_C
Cuts at: 256 4845 5093 256
Size: 4589 248 2578
Fragments arranged by size:
4589 2578 248
KpnI G_GTAC'C
Cuts at: 4435 4435
Size: 7415
MluI A'CGCG_T
Cuts at: 6848 6848
Size: 7415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1727 1850 3843 3948 6086 6270 1727
Size: 123 1993 105 2138 184 2872
Fragments arranged by size:
2872 2138 1993 184 123 105
MscI TGG'CCA
Cuts at: 1954 2371 2746 3700 5176 1954
Size: 417 375 954 1476 4193
Fragments arranged by size:
4193 1476 954 417 375
MslI CAynn'nnrTG
Cuts at: 469 1114 2188 3040 3221 3559 5404 5531 469
Size: 645 1074 852 181 338 1845 127 2353
Fragments arranged by size:
2353 1845 1074 852 645 338 181 127
MspA1I CmG'CkG
Cuts at: 2123 3011 3103 3447 3621 3909 4113 4526 4840
Size: 888 92 344 174 288 204 413 314
Cuts at: 4840 5200 6213 6458 2123
Size: 360 1013 245 3080
Fragments arranged by size:
3080 1013 888 413 360 344 314 288
245 204 174 92
NaeI GCC'GGC
Cuts at: 316 531 3599 3973 4254 5596 316
Size: 215 3068 374 281 1342 2135
Fragments arranged by size:
3068 2135 1342 374 281 215
NarI GG'CG_CC
Cuts at: 257 4846 5094 257
Size: 4589 248 2578
Fragments arranged by size:
4589 2578 248
NcoI C'CATG_G
Cuts at: 107 2483 3950 4181 107
Size: 2376 1467 231 3341
Fragments arranged by size:
3341 2376 1467 231
NgoMIV G'CCGG_C
Cuts at: 314 529 3597 3971 4252 5594 314
Size: 215 3068 374 281 1342 2135
Fragments arranged by size:
3068 2135 1342 374 281 215
NheI G'CTAG_C
Cuts at: 2322 2322
Size: 7415
NsiI A_TGCA'T
Cuts at: 1133 1639 1133
Size: 506 6909
NspI r_CATG'y
Cuts at: 123 592 1147 1833 2266 3672 4468 4968 5875
Size: 469 555 686 433 1406 796 500 907
Cuts at: 5875 123
Size: 1663
Fragments arranged by size:
1663 1406 907 796 686 555 500 469
433
PasI CC'CwG_GG
Cuts at: 483 1599 483
Size: 1116 6299
PciI A'CATG_T
Cuts at: 119 3668 5871 119
Size: 3549 2203 1663
Fragments arranged by size:
3549 2203 1663
PflMI CCAn_nnn'nTGG
Cuts at: 2010 2010
Size: 7415
PfoI T'CCnGG_A
Cuts at: 1940 2828 1940
Size: 888 6527
PmeI GTTT'AAAC
Cuts at: 4414 4414
Size: 7415
PmlI CAC'GTG
Cuts at: 384 384
Size: 7415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6554 6586 6554
Size: 32 7383
PpuMI rG'GwC_Cy
Cuts at: 452 487 568 663 1605 1788 2285 2906 3246
Size: 35 81 95 942 183 497 621 340
Cuts at: 3246 452
Size: 4621
Fragments arranged by size:
4621 942 621 497 340 183 95 81
35
PspOMI G'GGCC_C
Cuts at: 402 2704 402
Size: 2302 5113
PstI C_TGCA'G
Cuts at: 1397 3200 3446 3452 3635 4016 4106 4462 5147
Size: 1803 246 6 183 381 90 356 685
Cuts at: 5147 1397
Size: 3665
Fragments arranged by size:
3665 1803 685 381 356 246 183 90
6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7415
PvuII CAG'CTG
Cuts at: 3011 3103 3447 3621 4113 4840 5200 3011
Size: 92 344 174 492 727 360 5226
Fragments arranged by size:
5226 727 492 360 344 174 92
RsrII CG'GwC_CG
Cuts at: 5610 5610
Size: 7415
SacI G_AGCT'C
Cuts at: 1325 1721 2882 3371 4429 1325
Size: 396 1161 489 1058 4311
Fragments arranged by size:
4311 1161 1058 489 396
SalI G'TCGA_C
Cuts at: 4452 4452
Size: 7415
SanDI GG'GwC_CC
Cuts at: 452 487 568 1605 2285 452
Size: 35 81 1037 680 5582
Fragments arranged by size:
5582 1037 680 81 35
SapI GCTCTTCn'nnn_
Cuts at: 1189 1726 2632 5438 5648 1189
Size: 537 906 2806 210 2956
Fragments arranged by size:
2956 2806 906 537 210
SbfI CC_TGCA'GG
Cuts at: 4016 4106 4462 4016
Size: 90 356 6969
Fragments arranged by size:
6969 356 90
ScaI AGT'ACT
Cuts at: 3409 6932 3409
Size: 3523 3892
SfcI C'TryA_G
Cuts at: 33 1393 2037 3196 3442 3448 3631 3996 4012
Size: 1360 644 1159 246 6 183 365 16
Cuts at: 4012 4102 4458 5143 6136 6327 33
Size: 90 356 685 993 191 1121
Fragments arranged by size:
1360 1159 1121 993 685 644 365 356
246 191 183 90 16 6
SfoI GGC'GCC
Cuts at: 258 4847 5095 258
Size: 4589 248 2578
Fragments arranged by size:
4589 2578 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7415
SgrAI Cr'CCGG_yG
Cuts at: 4252 4252
Size: 7415
SmaI CCC'GGG
Cuts at: 573 4063 4437 573
Size: 3490 374 3551
Fragments arranged by size:
3551 3490 374
SmlI C'TyrA_G
Cuts at: 724 1024 1075 1309 3719 4570 5485 5977 6239
Size: 300 51 234 2410 851 915 492 262
Cuts at: 6239 724
Size: 1900
Fragments arranged by size:
2410 1900 915 851 492 300 262 234
51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7415
SphI G_CATG'C
Cuts at: 592 1147 4468 4968 592
Size: 555 3321 500 3039
Fragments arranged by size:
3321 3039 555 500
StuI AGG'CCT
Cuts at: 1155 3400 1155
Size: 2245 5170
StyI C'CwwG_G
Cuts at: 107 320 696 794 2471 2483 3950 4181 4549
Size: 213 376 98 1677 12 1467 231 368
Cuts at: 4549 4903 107
Size: 354 2619
Fragments arranged by size:
2619 1677 1467 376 368 354 231 213
98 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 899 2139 3140 3211 3831 4998 5131 5590 5799
Size: 1240 1001 71 620 1167 133 459 209
Cuts at: 5799 899
Size: 2515
Fragments arranged by size:
2515 1240 1167 1001 620 459 209 133
71
TatI w'GTAC_w
Cuts at: 1106 3407 4046 6930 1106
Size: 2301 639 2884 1591
Fragments arranged by size:
2884 2301 1591 639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 185 217 809 841 2801 2833 3806 3838 6932
Size: 32 592 32 1960 32 973 32 3094
Cuts at: 6932 6964 185
Size: 32 636
Fragments arranged by size:
3094 1960 973 636 592 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 886 5212 886
Size: 4326 3089
XbaI T'CTAG_A
Cuts at: 4446 4446
Size: 7415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 1251 4910 501
Size: 750 3659 3006
Fragments arranged by size:
3659 3006 750
XhoI C'TCGA_G
Cuts at: 3719 3719
Size: 7415
XmaI C'CCGG_G
Cuts at: 571 4061 4435 571
Size: 3490 374 3551
Fragments arranged by size:
3551 3490 374
XmnI GAAnn'nnTTC
Cuts at: 2923 2923
Size: 7415
ZraI GAC'GTC
Cuts at: 1164 1985 4176 1164
Size: 821 2191 4403
Fragments arranged by size:
4403 2191 821
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BcgI BmgBI BssHII BstZ17I
ClaI CspCI FspAI HpaI MfeI NdeI NotI NruI
PacI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SnaBI SrfI SspI SwaI