Restriction map for
pF1KE2322
(Circular) (Six-base) MAPSORT of: pF1KE2322.seq Check: 3139 from: 1 to: 7118
pF1KE2322 7118 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1410 1410
Size: 7118
AccI GT'mk_AC
Cuts at: 686 3683 4156 686
Size: 2997 473 3648
Fragments arranged by size:
3648 2997 473
Acc65I G'GTAC_C
Cuts at: 4134 4134
Size: 7118
AclI AA'CG_TT
Cuts at: 6958 6958
Size: 7118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1101 1558 1753 3841 4943 5375 6122 6390 1101
Size: 457 195 2088 1102 432 747 268 1829
Fragments arranged by size:
2088 1829 1102 747 457 432 268 195
AfeI AGC'GCT
Cuts at: 1905 1905
Size: 7118
AflIII A'CryG_T
Cuts at: 4470 5574 6551 4470
Size: 1104 977 5037
Fragments arranged by size:
5037 1104 977
AgeI A'CCGG_T
Cuts at: 5506 5506
Size: 7118
AleI CACnn'nnGTG
Cuts at: 1984 1984
Size: 7118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2579 2579
Size: 7118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3064 3096 3064
Size: 32 7086
AlwNI CAG_nnn'CTG
Cuts at: 756 1425 1859 2331 3245 3392 4423 5990 756
Size: 669 434 472 914 147 1031 1567 1884
Fragments arranged by size:
1884 1567 1031 914 669 472 434 147
ApaI G_GGCC'C
Cuts at: 459 2908 459
Size: 2449 4669
ApaLI G'TGCA_C
Cuts at: 350 1438 2244 2713 5888 350
Size: 1088 806 469 3175 1580
Fragments arranged by size:
3175 1580 1088 806 469
ApoI r'AATT_y
Cuts at: 420 1152 3196 3399 4122 6471 6727 420
Size: 732 2044 203 723 2349 256 811
Fragments arranged by size:
2349 2044 811 732 723 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 7118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7118
AvaI C'yCGr_G
Cuts at: 1094 1559 1945 2422 4138 1094
Size: 465 386 477 1716 4074
Fragments arranged by size:
4074 1716 477 465 386
BamHI G'GATC_C
Cuts at: 4143 4143
Size: 7118
BanI G'GyrC_C
Cuts at: 570 966 2827 3379 4012 4134 4548 4796 4831
Size: 396 1861 552 633 122 414 248 35
Cuts at: 4831 570
Size: 2857
Fragments arranged by size:
2857 1861 633 552 414 396 248 122
35
BanII G_rGCy'C
Cuts at: 459 1555 2077 2272 2737 2908 3452 3529 3965
Size: 1096 522 195 465 171 544 77 436
Cuts at: 3965 4132 5162 459
Size: 167 1030 2415
Fragments arranged by size:
2415 1096 1030 544 522 465 436 195
171 167 77
BbeI G_GCGC'C
Cuts at: 4016 4552 4800 4016
Size: 536 248 6334
Fragments arranged by size:
6334 536 248
BbsI GAAGACnn'nnnn_
Cuts at: 280 343 597 1959 280
Size: 63 254 1362 5439
Fragments arranged by size:
5439 1362 254 63
BbvCI CC'TCA_GC
Cuts at: 2383 2383
Size: 7118
BciVI GTATCCnnnnn_n'
Cuts at: 5016 5777 7118 5016
Size: 761 1341 5016
Fragments arranged by size:
5016 1341 761
BclI T'GATC_A
Cuts at: 4638 4638
Size: 7118
BglI GCCn_nnn'nGGC
Cuts at: 847 2626 2825 847
Size: 1779 199 5140
Fragments arranged by size:
5140 1779 199
BglII A'GATC_T
Cuts at: 4633 6670 4633
Size: 2037 5081
BlpI GC'TnA_GC
Cuts at: 4230 4230
Size: 7118
Bme1580I G_kGCm'C
Cuts at: 354 459 575 1442 2248 2717 2830 2908 4743
Size: 105 116 867 806 469 113 78 1835
Cuts at: 4743 4836 5892 354
Size: 93 1056 1580
Fragments arranged by size:
1835 1580 1056 867 806 469 116 113
105 93 78
BmgBI CAC'GTC
Cuts at: 512 659 2592 3537 512
Size: 147 1933 945 4093
Fragments arranged by size:
4093 1933 945 147
BmrI ACTGGGnnnn_n'
Cuts at: 2221 3267 3507 4330 4411 4746 6890 2221
Size: 1046 240 823 81 335 2144 2449
Fragments arranged by size:
2449 2144 1046 823 335 240 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5485 5485
Size: 7118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 778 1531 1816 2180 2748 3012 3067 273
Size: 505 753 285 364 568 264 55 4324
Fragments arranged by size:
4324 753 568 505 364 285 264 55
Bpu10I CC'TnA_GC
Cuts at: 240 2383 3528 240
Size: 2143 1145 3830
Fragments arranged by size:
3830 2143 1145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4294 5173 5665 5963 4294
Size: 879 492 298 5449
Fragments arranged by size:
5449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2446 2589 3502 6822 2446
Size: 143 913 3320 2742
Fragments arranged by size:
3320 2742 913 143
BsaAI yAC'GTr
Cuts at: 1226 1533 4471 1226
Size: 307 2938 3873
Fragments arranged by size:
3873 2938 307
BsaBI GATnn'nnATC
Cuts at: 6 4632 6669 6
Size: 4626 2037 455
Fragments arranged by size:
4626 2037 455
BsaHI Gr'CG_yC
Cuts at: 974 1027 2746 3179 4013 4549 4797 5499 6995
Size: 53 1719 433 834 536 248 702 1496
Cuts at: 6995 974
Size: 1097
Fragments arranged by size:
1719 1496 1097 834 702 536 433 248
53
BsaWI w'CCGG_w
Cuts at: 1700 2084 3548 4307 4828 5506 5780 5927 6338
Size: 384 1464 759 521 678 274 147 411
Cuts at: 6338 1700
Size: 2480
Fragments arranged by size:
2480 1464 759 678 521 411 384 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 679 709 2653 2683 3058 3064 3088 3094 4066
Size: 30 1944 30 375 6 24 6 972
Cuts at: 4066 4096 679
Size: 30 3701
Fragments arranged by size:
3701 1944 972 375 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 1918 2446 2478 2658 3491 3508 550
Size: 1368 528 32 180 833 17 4160
Fragments arranged by size:
4160 1368 833 528 180 32 17
BseYI C'CCAG_C
Cuts at: 2054 2842 3011 3113 3481 4543 5301 5878 6374
Size: 788 169 102 368 1062 758 577 496
Cuts at: 6374 2054
Size: 2798
Fragments arranged by size:
2798 1062 788 758 577 496 368 169
102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 1176 2127 2539 2992 3601 492
Size: 684 951 412 453 609 4009
Fragments arranged by size:
4009 951 684 609 453 412
BsiEI CG_ry'CG
Cuts at: 104 2647 2824 4660 4706 5914 104
Size: 2543 177 1836 46 1208 1308
Fragments arranged by size:
2543 1836 1308 1208 177 46
BsiHKAI G_wGCw'C
Cuts at: 354 1091 1442 1555 2248 2272 2717 3041 3529
Size: 737 351 113 693 24 445 324 488
Cuts at: 3529 3737 3965 4132 4910 5100 5892 354
Size: 208 228 167 778 190 792 1580
Fragments arranged by size:
1580 792 778 737 693 488 445 351
324 228 208 190 167 113 24
BsmI GAATG_Cn'
Cuts at: 367 1351 3715 367
Size: 984 2364 3770
Fragments arranged by size:
3770 2364 984
BsmBI CGTCTCn'nnnn_
Cuts at: 3527 3527
Size: 7118
Bsp1286I G_dGCh'C
Cuts at: 354 459 575 1091 1442 1555 2077 2248 2272
Size: 105 116 516 351 113 522 171 24
Cuts at: 2272 2717 2737 2830 2908 3041 3452 3529 3737
Size: 445 20 93 78 133 411 77 208
Cuts at: 3737 3965 4132 4743 4836 4910 5100 5162 5892
Size: 228 167 611 93 74 190 62 730
Cuts at: 5892 354
Size: 1580
Fragments arranged by size:
1580 730 611 522 516 445 411 351
228 208 190 171 167 133 116 113
105 93 93 78 77 74 62 24
20
BspEI T'CCGG_A
Cuts at: 2084 3548 6338 2084
Size: 1464 2790 2864
Fragments arranged by size:
2864 2790 1464
BspHI T'CATG_A
Cuts at: 197 1908 3020 3521 3719 6294 197
Size: 1711 1112 501 198 2575 1021
Fragments arranged by size:
2575 1711 1112 1021 501 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 659 1410 4168 4382 4684 5065 659
Size: 751 2758 214 302 381 2712
Fragments arranged by size:
2758 2712 751 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3069 3641 5410 5464 6948 7113 50
Size: 3019 572 1769 54 1484 165 55
Fragments arranged by size:
3019 1769 1484 572 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 5030 1781
Size: 3249 3869
BsrFI r'CCGG_y
Cuts at: 1442 2793 2819 3511 5116 5297 5506 1442
Size: 1351 26 692 1605 181 209 3054
Fragments arranged by size:
3054 1605 1351 692 209 181 26
BssSI C'ACGA_G
Cuts at: 1617 1633 3309 5389 5747 1617
Size: 16 1676 2080 358 2988
Fragments arranged by size:
2988 2080 1676 358 16
BstAPI GCAn_nnn'nTGC
Cuts at: 1821 2141 4495 1821
Size: 320 2354 4444
Fragments arranged by size:
4444 2354 320
BstBI TT'CG_AA
Cuts at: 1149 5479 1149
Size: 4330 2788
BstEII G'GTnAC_C
Cuts at: 3432 3432
Size: 7118
BstXI CCAn_nnnn'nTGG
Cuts at: 1377 1794 2049 2781 1377
Size: 417 255 732 5714
Fragments arranged by size:
5714 732 417 255
BstYI r'GATC_y
Cuts at: 1 998 1049 1098 1598 1856 3026 3056 3551
Size: 997 51 49 500 258 1170 30 495
Cuts at: 3551 4143 4610 4633 4968 5214 6226 6312 6324
Size: 592 467 23 335 246 1012 86 12
Cuts at: 6324 6670 1
Size: 346 449
Fragments arranged by size:
1170 1012 997 592 500 495 467 449
346 335 258 246 86 51 49 30
23 12
BtgI C'CryG_G
Cuts at: 107 564 1370 2078 2147 2966 3323 4058 107
Size: 457 806 708 69 819 357 735 3167
Fragments arranged by size:
3167 819 806 735 708 457 357 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1059 2601 3204 5068 5249 5412 1059
Size: 1542 603 1864 181 163 2765
Fragments arranged by size:
2765 1864 1542 603 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1502 1613 3317 4361 1307
Size: 195 111 1704 1044 4064
Fragments arranged by size:
4064 1704 1044 195 111
ClaI AT'CG_AT
Cuts at: 371 371
Size: 7118
DraI TTT'AAA
Cuts at: 4117 4117
Size: 7118
DraIII CAC_nnn'GTG
Cuts at: 570 2037 3215 3789 570
Size: 1467 1178 574 3899
Fragments arranged by size:
3899 1467 1178 574
DrdI GACnn_nn'nnGTC
Cuts at: 3621 4824 5682 3621
Size: 1203 858 5057
Fragments arranged by size:
5057 1203 858
EaeI y'GGCC_r
Cuts at: 561 1005 1570 1647 1838 2039 2594 2644 2783
Size: 444 565 77 191 201 555 50 139
Cuts at: 2783 2817 2821 3339 3365 3780 4055 4703 4877
Size: 34 4 518 26 415 275 648 174
Cuts at: 4877 5268 5295 6640 561
Size: 391 27 1345 1039
Fragments arranged by size:
1345 1039 648 565 555 518 444 415
391 275 201 191 174 139 77 50
34 27 26 4
EagI C'GGCC_G
Cuts at: 2644 2821 4703 2644
Size: 177 1882 5059
Fragments arranged by size:
5059 1882 177
EarI CTCTTCn'nnn_
Cuts at: 140 1108 1120 2162 3029 5141 5351 140
Size: 968 12 1042 867 2112 210 1907
Fragments arranged by size:
2112 1907 1042 968 867 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1180 1642 1690 2952 3916 5326 5636 5782 6928
Size: 462 48 1262 964 1410 310 146 1146
Cuts at: 6928 1180
Size: 1370
Fragments arranged by size:
1410 1370 1262 1146 964 462 310 146
48
EcoICRI GAG'CTC
Cuts at: 1553 2270 3527 3963 4130 1553
Size: 717 1257 436 167 4541
Fragments arranged by size:
4541 1257 717 436 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 778 1101 1531 1558 1753 1816 2180 2748
Size: 505 323 430 27 195 63 364 568
Cuts at: 2748 3012 3067 3841 4943 5375 6122 6390 273
Size: 264 55 774 1102 432 747 268 1001
Fragments arranged by size:
1102 1001 774 747 568 505 432 430
364 323 268 264 195 63 55 27
EcoNI CCTnn'n_nnAGG
Cuts at: 148 1636 148
Size: 1488 5630
EcoO109I rG'GnC_Cy
Cuts at: 388 455 1254 1486 2836 2904 4257 388
Size: 67 799 232 1350 68 1353 3249
Fragments arranged by size:
3249 1353 1350 799 232 68 67
EcoRI G'AATT_C
Cuts at: 4122 4122
Size: 7118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 5137 1783
Size: 3354 3764
FseI GG_CCGG'CC
Cuts at: 2823 2823
Size: 7118
FspI TGC'GCA
Cuts at: 1959 3817 4899 1959
Size: 1858 1082 4178
Fragments arranged by size:
4178 1858 1082
FspAI rTGC'GCAy
Cuts at: 3817 3817
Size: 7118
HaeII r_GCGC'y
Cuts at: 1907 2402 2999 4016 4552 4800 5822 6801 1907
Size: 495 597 1017 536 248 1022 979 2224
Fragments arranged by size:
2224 1022 1017 979 597 536 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 360 364 375 392 396 407 2653
Size: 32 79 4 11 17 4 11 2246
Cuts at: 2653 2685 3950 3982 4960 4990 4992 5022 249
Size: 32 1265 32 978 30 2 30 2345
Fragments arranged by size:
2345 2246 1265 978 79 32 32 32
30 30 17 11 11 4 4 2
HincII GTy'rAC
Cuts at: 588 1266 1737 4157 588
Size: 678 471 2420 3549
Fragments arranged by size:
3549 2420 678 471
KasI G'GCGC_C
Cuts at: 4012 4548 4796 4012
Size: 536 248 6334
Fragments arranged by size:
6334 536 248
KpnI G_GTAC'C
Cuts at: 4138 4138
Size: 7118
MluI A'CGCG_T
Cuts at: 6551 6551
Size: 7118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1958 5789 5973 1958
Size: 3831 184 3103
Fragments arranged by size:
3831 3103 184
MscI TGG'CCA
Cuts at: 563 1840 2041 2596 2785 3341 4879 563
Size: 1277 201 555 189 556 1538 2802
Fragments arranged by size:
2802 1538 1277 556 555 201 189
MslI CAynn'nnrTG
Cuts at: 557 1228 1375 1984 3322 4084 5107 5234 557
Size: 671 147 609 1338 762 1023 127 2441
Fragments arranged by size:
2441 1338 1023 762 671 609 147 127
MspA1I CmG'CkG
Cuts at: 744 834 1284 1344 1404 1693 1955 1963 2058
Size: 90 450 60 60 289 262 8 95
Cuts at: 2058 2842 3608 3956 4005 4022 4229 4543 4903
Size: 784 766 348 49 17 207 314 360
Cuts at: 4903 5916 6161 744
Size: 1013 245 1701
Fragments arranged by size:
1701 1013 784 766 450 360 348 314
289 262 245 207 95 90 60 60
49 17 8
NaeI GCC'GGC
Cuts at: 2821 5299 2821
Size: 2478 4640
NarI GG'CG_CC
Cuts at: 4013 4549 4797 4013
Size: 536 248 6334
Fragments arranged by size:
6334 536 248
NcoI C'CATG_G
Cuts at: 107 1370 2078 3323 107
Size: 1263 708 1245 3902
Fragments arranged by size:
3902 1263 1245 708
NgoMIV G'CCGG_C
Cuts at: 2819 5297 2819
Size: 2478 4640
NspI r_CATG'y
Cuts at: 948 1233 1689 3462 3917 4171 4671 5578 948
Size: 285 456 1773 455 254 500 907 2488
Fragments arranged by size:
2488 1773 907 500 456 455 285 254
PasI CC'CwG_GG
Cuts at: 1032 1490 2412 1032
Size: 458 922 5738
Fragments arranged by size:
5738 922 458
PciI A'CATG_T
Cuts at: 5574 5574
Size: 7118
PflMI CCAn_nnn'nTGG
Cuts at: 1079 1836 2037 1079
Size: 757 201 6160
Fragments arranged by size:
6160 757 201
PfoI T'CCnGG_A
Cuts at: 2657 2657
Size: 7118
PmeI GTTT'AAAC
Cuts at: 4117 4117
Size: 7118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1858 1890 3064 3096 6257 6289 1858
Size: 32 1174 32 3161 32 2687
Fragments arranged by size:
3161 2687 1174 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 388 1254 1486 388
Size: 866 232 6020
Fragments arranged by size:
6020 866 232
PshAI GACnn'nnGTC
Cuts at: 1271 1484 1271
Size: 213 6905
PspOMI G'GGCC_C
Cuts at: 455 2904 455
Size: 2449 4669
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3289 3321 3289
Size: 32 7086
PstI C_TGCA'G
Cuts at: 656 1421 2001 2159 3767 4165 4850 656
Size: 765 580 158 1608 398 685 2924
Fragments arranged by size:
2924 1608 765 685 580 398 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7118
PvuII CAG'CTG
Cuts at: 834 1284 1344 1404 1693 2058 3608 4022 4543
Size: 450 60 60 289 365 1550 414 521
Cuts at: 4543 4903 834
Size: 360 3049
Fragments arranged by size:
3049 1550 521 450 414 365 360 289
60 60
RsrII CG'GwC_CG
Cuts at: 5313 5313
Size: 7118
SacI G_AGCT'C
Cuts at: 1555 2272 3529 3965 4132 1555
Size: 717 1257 436 167 4541
Fragments arranged by size:
4541 1257 717 436 167
SalI G'TCGA_C
Cuts at: 4155 4155
Size: 7118
SanDI GG'GwC_CC
Cuts at: 1486 1486
Size: 7118
SapI GCTCTTCn'nnn_
Cuts at: 1120 3029 5141 5351 1120
Size: 1909 2112 210 2887
Fragments arranged by size:
2887 2112 1909 210
SbfI CC_TGCA'GG
Cuts at: 1421 4165 1421
Size: 2744 4374
ScaI AGT'ACT
Cuts at: 1657 2606 3982 6635 1657
Size: 949 1376 2653 2140
Fragments arranged by size:
2653 2140 1376 949
SfcI C'TryA_G
Cuts at: 33 652 739 1417 1920 1997 2155 3224 3763
Size: 619 87 678 503 77 158 1069 539
Cuts at: 3763 4161 4846 5839 6030 33
Size: 398 685 993 191 1121
Fragments arranged by size:
1121 1069 993 685 678 619 539 503
398 191 158 87 77
SfoI GGC'GCC
Cuts at: 4014 4550 4798 4014
Size: 536 248 6334
Fragments arranged by size:
6334 536 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7118
SmaI CCC'GGG
Cuts at: 1096 4140 1096
Size: 3044 4074
SmlI C'TyrA_G
Cuts at: 4273 5188 5680 5942 4273
Size: 915 492 262 5449
Fragments arranged by size:
5449 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7118
SphI G_CATG'C
Cuts at: 948 4171 4671 948
Size: 3223 500 3395
Fragments arranged by size:
3395 3223 500
SspI AAT'ATT
Cuts at: 3158 3158
Size: 7118
StuI AGG'CCT
Cuts at: 3757 3757
Size: 7118
StyI C'CwwG_G
Cuts at: 107 451 530 1370 1974 2078 3323 4252 4606
Size: 344 79 840 604 104 1245 929 354
Cuts at: 4606 107
Size: 2619
Fragments arranged by size:
2619 1245 929 840 604 354 344 104
79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1024 1656 1824 3735 4701 4834 5293 5502 1024
Size: 632 168 1911 966 133 459 209 2640
Fragments arranged by size:
2640 1911 966 632 459 209 168 133
TatI w'GTAC_w
Cuts at: 626 1655 2604 2852 3980 4088 6633 626
Size: 1029 949 248 1128 108 2545 1111
Fragments arranged by size:
2545 1128 1111 1029 949 248 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1603 1635 3058 3090 6635 6667 1603
Size: 32 1423 32 3545 32 2054
Fragments arranged by size:
3545 2054 1423 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4915 4915
Size: 7118
XbaI T'CTAG_A
Cuts at: 4149 4149
Size: 7118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2409 4023 4613 2409
Size: 1614 590 4914
Fragments arranged by size:
4914 1614 590
XmaI C'CCGG_G
Cuts at: 1094 4138 1094
Size: 3044 4074
XmnI GAAnn'nnTTC
Cuts at: 2893 2893
Size: 7118
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I CspCI EcoRV
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI