Restriction map for
pF1KE2327
(Circular) (Six-base) MAPSORT of: pF1KE2327.seq Check: 2186 from: 1 to: 6050
pF1KE2327 6050 bp
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AccI GT'mk_AC
Cuts at: 2921 3088 2921
Size: 167 5883
Acc65I G'GTAC_C
Cuts at: 3066 3066
Size: 6050
AclI AA'CG_TT
Cuts at: 1431 5890 1431
Size: 4459 1591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1039 3875 4307 5054 5322 506
Size: 533 2836 432 747 268 1234
Fragments arranged by size:
2836 1234 747 533 432 268
AflIII A'CryG_T
Cuts at: 3402 4506 5483 3402
Size: 1104 977 3969
Fragments arranged by size:
3969 1104 977
AgeI A'CCGG_T
Cuts at: 4438 4438
Size: 6050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2259 2259
Size: 6050
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1005 1037 1005
Size: 32 6018
AlwNI CAG_nnn'CTG
Cuts at: 402 2400 2948 3355 4922 402
Size: 1998 548 407 1567 1530
Fragments arranged by size:
1998 1567 1530 548 407
ApaLI G'TGCA_C
Cuts at: 4820 4820
Size: 6050
ApoI r'AATT_y
Cuts at: 405 759 986 1524 1660 1997 2490 2555 2565
Size: 354 227 538 136 337 493 65 10
Cuts at: 2565 3054 5403 5659 405
Size: 489 2349 256 796
Fragments arranged by size:
2349 796 538 493 489 354 337 256
227 136 65 10
AseI AT'TA_AT
Cuts at: 20 2080 20
Size: 2060 3990
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 268 3070 268
Size: 2802 3248
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 473 506 473
Size: 33 6017
BamHI G'GATC_C
Cuts at: 3075 3075
Size: 6050
BanI G'GyrC_C
Cuts at: 272 3066 3480 3728 3763 272
Size: 2794 414 248 35 2559
Fragments arranged by size:
2794 2559 414 248 35
BanII G_rGCy'C
Cuts at: 3064 4094 3064
Size: 1030 5020
BbeI G_GCGC'C
Cuts at: 3484 3732 3484
Size: 248 5802
BbsI GAAGACnn'nnnn_
Cuts at: 1963 2372 1963
Size: 409 5641
BciVI GTATCCnnnnn_n'
Cuts at: 469 3948 4709 6050 469
Size: 3479 761 1341 469
Fragments arranged by size:
3479 1341 761 469
BclI T'GATC_A
Cuts at: 2978 3570 2978
Size: 592 5458
BglII A'GATC_T
Cuts at: 3565 5602 3565
Size: 2037 4013
BlpI GC'TnA_GC
Cuts at: 2426 3162 2426
Size: 736 5314
Bme1580I G_kGCm'C
Cuts at: 275 3675 3768 4824 275
Size: 3400 93 1056 1501
Fragments arranged by size:
3400 1501 1056 93
BmgBI CAC'GTC
Cuts at: 2335 2335
Size: 6050
BmrI ACTGGGnnnn_n'
Cuts at: 141 2251 2320 3262 3343 3678 5822 141
Size: 2110 69 942 81 335 2144 369
Fragments arranged by size:
2144 2110 942 369 335 81 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4417 4417
Size: 6050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1048 2819 979
Size: 69 1771 4210
Fragments arranged by size:
4210 1771 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 3226 4105 4597 4895 806
Size: 2420 879 492 298 1961
Fragments arranged by size:
2420 1961 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5754 5754
Size: 6050
BsaAI yAC'GTr
Cuts at: 3403 3403
Size: 6050
BsaBI GATnn'nnATC
Cuts at: 6 773 1982 2789 3564 5601 6
Size: 767 1209 807 775 2037 455
Fragments arranged by size:
2037 1209 807 775 767 455
BsaHI Gr'CG_yC
Cuts at: 3481 3729 4431 5927 3481
Size: 248 702 1496 3604
Fragments arranged by size:
3604 1496 702 248
BsaWI w'CCGG_w
Cuts at: 160 214 1281 3239 3760 4438 4712 4859 5270
Size: 54 1067 1958 521 678 274 147 411
Cuts at: 5270 160
Size: 940
Fragments arranged by size:
1958 1067 940 678 521 411 274 147
54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 196 226 196
Size: 30 6020
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 233
Size: 6050
BseYI C'CCAG_C
Cuts at: 1598 2315 2363 3475 4233 4810 5306 1598
Size: 717 48 1112 758 577 496 2342
Fragments arranged by size:
2342 1112 758 717 577 496 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2709 2972 2709
Size: 263 5787
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4846 104
Size: 3488 46 1208 1308
Fragments arranged by size:
3488 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2238 3064 3842 4032 4824 2238
Size: 826 778 190 792 3464
Fragments arranged by size:
3464 826 792 778 190
BsmI GAATG_Cn'
Cuts at: 746 1221 746
Size: 475 5575
BsmBI CGTCTCn'nnnn_
Cuts at: 997 1822 997
Size: 825 5225
Bsp1286I G_dGCh'C
Cuts at: 275 2238 3064 3675 3768 3842 4032 4094 4824
Size: 1963 826 611 93 74 190 62 730
Cuts at: 4824 275
Size: 1501
Fragments arranged by size:
1963 1501 826 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 214 5270 214
Size: 5056 994
BspHI T'CATG_A
Cuts at: 5226 5226
Size: 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3100 3314 3616 3997 3100
Size: 214 302 381 5153
Fragments arranged by size:
5153 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4342 4396 5880 6045 50
Size: 4292 54 1484 165 55
Fragments arranged by size:
4292 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1763 1935 3962 1763
Size: 172 2027 3851
Fragments arranged by size:
3851 2027 172
BsrFI r'CCGG_y
Cuts at: 260 4048 4229 4438 260
Size: 3788 181 209 1872
Fragments arranged by size:
3788 1872 209 181
BsrGI T'GTAC_A
Cuts at: 393 1036 393
Size: 643 5407
BssSI C'ACGA_G
Cuts at: 4321 4679 4321
Size: 358 5692
BstAPI GCAn_nnn'nTGC
Cuts at: 2400 2845 3427 2400
Size: 445 582 5023
Fragments arranged by size:
5023 582 445
BstBI TT'CG_AA
Cuts at: 4411 4411
Size: 6050
BstEII G'GTnAC_C
Cuts at: 202 202
Size: 6050
BstXI CCAn_nnnn'nTGG
Cuts at: 2315 2315
Size: 6050
BstYI r'GATC_y
Cuts at: 1 868 2292 2472 3075 3542 3565 3900 4146
Size: 867 1424 180 603 467 23 335 246
Cuts at: 4146 5158 5244 5256 5602 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1424 1012 867 603 467 449 346 335
246 180 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 464 464
Size: 6050
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 969 2111 2389 3293 969
Size: 1142 278 904 3726
Fragments arranged by size:
3726 1142 904 278
DraI TTT'AAA
Cuts at: 1650 1719 3049 1650
Size: 69 1330 4651
Fragments arranged by size:
4651 1330 69
DrdI GACnn_nn'nnGTC
Cuts at: 3756 4614 3756
Size: 858 5192
EaeI y'GGCC_r
Cuts at: 1435 3635 3809 4200 4227 5572 1435
Size: 2200 174 391 27 1345 1913
Fragments arranged by size:
2200 1913 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 1559 1914 2315 4073 4283 1559
Size: 355 401 1758 210 3326
Fragments arranged by size:
3326 1758 401 355 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 248 3005 4258 4568 4714 5860 248
Size: 2757 1253 310 146 1146 438
Fragments arranged by size:
2757 1253 1146 438 310 146
EcoICRI GAG'CTC
Cuts at: 3062 3062
Size: 6050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 979 1039 1048 2819 3875 4307 5054 5322
Size: 473 60 9 1771 1056 432 747 268
Cuts at: 5322 506
Size: 1234
Fragments arranged by size:
1771 1234 1056 747 473 432 268 60
9
EcoO109I rG'GnC_Cy
Cuts at: 148 3189 148
Size: 3041 3009
EcoRI G'AATT_C
Cuts at: 405 3054 405
Size: 2649 3401
EcoRV GAT'ATC
Cuts at: 449 982 449
Size: 533 5517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4069 4069
Size: 6050
FspI TGC'GCA
Cuts at: 3831 3831
Size: 6050
HaeII r_GCGC'y
Cuts at: 3484 3732 4754 5733 3484
Size: 248 1022 979 3801
Fragments arranged by size:
3801 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 1893 1925 2077 2109 2464 2496 2892
Size: 32 1443 32 152 32 355 32 396
Cuts at: 2892 2923 2924 2955 3892 3922 3924 3954 418
Size: 31 1 31 937 30 2 30 2514
Fragments arranged by size:
2514 1443 937 396 355 152 32 32
32 32 31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 1701 3089 1701
Size: 1388 4662
HpaI GTT'AAC
Cuts at: 1701 1701
Size: 6050
KasI G'GCGC_C
Cuts at: 3480 3728 3480
Size: 248 5802
KpnI G_GTAC'C
Cuts at: 3070 3070
Size: 6050
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4721 4905 4721
Size: 184 5866
MscI TGG'CCA
Cuts at: 1437 3811 1437
Size: 2374 3676
MslI CAynn'nnrTG
Cuts at: 368 4039 4166 368
Size: 3671 127 2252
Fragments arranged by size:
3671 2252 127
MspA1I CmG'CkG
Cuts at: 1870 3161 3475 3835 4848 5093 1870
Size: 1291 314 360 1013 245 2827
Fragments arranged by size:
2827 1291 1013 360 314 245
NaeI GCC'GGC
Cuts at: 262 4231 262
Size: 3969 2081
NarI GG'CG_CC
Cuts at: 3481 3729 3481
Size: 248 5802
NgoMIV G'CCGG_C
Cuts at: 260 4229 260
Size: 3969 2081
NspI r_CATG'y
Cuts at: 300 1387 3103 3603 4510 300
Size: 1087 1716 500 907 1840
Fragments arranged by size:
1840 1716 1087 907 500
PciI A'CATG_T
Cuts at: 4506 4506
Size: 6050
PflMI CCAn_nnn'nTGG
Cuts at: 2212 2212
Size: 6050
PfoI T'CCnGG_A
Cuts at: 533 3026 533
Size: 2493 3557
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 5189
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 148 148
Size: 6050
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 377 409 2012 2044 377
Size: 32 1603 32 4383
Fragments arranged by size:
4383 1603 32 32
PstI C_TGCA'G
Cuts at: 942 1133 2396 3097 3782 942
Size: 191 1263 701 685 3210
Fragments arranged by size:
3210 1263 701 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 3475 3835 3475
Size: 360 5690
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 3064 3064
Size: 6050
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SanDI GG'GwC_CC
Cuts at: 148 148
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 4073 4283 4073
Size: 210 5840
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 975 1479 2261 2957 5567 975
Size: 504 782 696 2610 1458
Fragments arranged by size:
2610 1458 782 696 504
SexAI A'CCwGG_T
Cuts at: 1266 1929 1266
Size: 663 5387
SfcI C'TryA_G
Cuts at: 33 356 938 1129 1481 1694 2392 2807 2918
Size: 323 582 191 352 213 698 415 111
Cuts at: 2918 3093 3778 4771 4962 33
Size: 175 685 993 191 1121
Fragments arranged by size:
1121 993 698 685 582 415 352 323
213 191 191 175 111
SfoI GGC'GCC
Cuts at: 3482 3730 3482
Size: 248 5802
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 270 3072 270
Size: 2802 3248
SmlI C'TyrA_G
Cuts at: 821 3205 4120 4612 4874 821
Size: 2384 915 492 262 1997
Fragments arranged by size:
2384 1997 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 3103 3603 3103
Size: 500 5550
SspI AAT'ATT
Cuts at: 1196 1237 2371 1196
Size: 41 1134 4875
Fragments arranged by size:
4875 1134 41
StyI C'CwwG_G
Cuts at: 1009 3184 3538 1009
Size: 2175 354 3521
Fragments arranged by size:
3521 2175 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 614 3633 3766 4225 4434 614
Size: 3019 133 459 209 2230
Fragments arranged by size:
3019 2230 459 209 133
TatI w'GTAC_w
Cuts at: 360 393 889 973 1036 1477 1858 2259 2955
Size: 33 496 84 63 441 381 401 696
Cuts at: 2955 5565 360
Size: 2610 845
Fragments arranged by size:
2610 845 696 496 441 401 381 84
63 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5567 5599 5567
Size: 32 6018
Tth111I GACn'n_nGTC
Cuts at: 3847 3847
Size: 6050
XbaI T'CTAG_A
Cuts at: 1974 3081 1974
Size: 1107 4943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3545 3545
Size: 6050
XmaI C'CCGG_G
Cuts at: 268 3070 268
Size: 2802 3248
XmnI GAAnn'nnTTC
Cuts at: 1467 2372 1467
Size: 905 5145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BssHII BstZ17I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
SacII SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI