Restriction map for
pF1KE2332
(Circular) (Six-base) MAPSORT of: pF1KE2332.seq Check: 8813 from: 1 to: 4766
pF1KE2332 4766 bp
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AccI GT'mk_AC
Cuts at: 877 1804 877
Size: 927 3839
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 4606 4606
Size: 4766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 580 740 2591 3023 3770 4038 368
Size: 212 160 1851 432 747 268 1096
Fragments arranged by size:
1851 1096 747 432 268 212 160
AflII C'TTAA_G
Cuts at: 1351 1351
Size: 4766
AflIII A'CryG_T
Cuts at: 661 996 1756 2118 3222 4199 661
Size: 335 760 362 1104 977 1228
Fragments arranged by size:
1228 1104 977 760 362 335
AgeI A'CCGG_T
Cuts at: 3154 3154
Size: 4766
AhdI GACnn_n'nnGTC
Cuts at: 636 636
Size: 4766
AleI CACnn'nnGTG
Cuts at: 1757 1757
Size: 4766
AlwNI CAG_nnn'CTG
Cuts at: 2071 3638 2071
Size: 1567 3199
ApaLI G'TGCA_C
Cuts at: 3536 3536
Size: 4766
ApoI r'AATT_y
Cuts at: 259 378 753 1207 1770 4119 4375 259
Size: 119 375 454 563 2349 256 650
Fragments arranged by size:
2349 650 563 454 375 256 119
AseI AT'TA_AT
Cuts at: 20 922 1336 20
Size: 902 414 3450
Fragments arranged by size:
3450 902 414
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 1786 1786
Size: 4766
BamHI G'GATC_C
Cuts at: 1791 1791
Size: 4766
BanI G'GyrC_C
Cuts at: 837 1782 2196 2444 2479 837
Size: 945 414 248 35 3124
Fragments arranged by size:
3124 945 414 248 35
BanII G_rGCy'C
Cuts at: 1477 1780 2810 1477
Size: 303 1030 3433
Fragments arranged by size:
3433 1030 303
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3425 4766 2664
Size: 761 1341 2664
Fragments arranged by size:
2664 1341 761
BclI T'GATC_A
Cuts at: 1053 2286 1053
Size: 1233 3533
BglII A'GATC_T
Cuts at: 829 1347 2281 4318 829
Size: 518 934 2037 1277
Fragments arranged by size:
2037 1277 934 518
BlpI GC'TnA_GC
Cuts at: 1878 1878
Size: 4766
Bme1580I G_kGCm'C
Cuts at: 2391 2484 3540 2391
Size: 93 1056 3617
Fragments arranged by size:
3617 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1978 2059 2394 4538 1978
Size: 81 335 2144 2206
Fragments arranged by size:
2206 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 4766
Bpu10I CC'TnA_GC
Cuts at: 930 930
Size: 4766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 1942 2821 3313 3611 1150
Size: 792 879 492 298 2305
Fragments arranged by size:
2305 879 792 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 1105 4470 805
Size: 300 3365 1101
Fragments arranged by size:
3365 1101 300
BsaAI yAC'GTr
Cuts at: 662 2119 662
Size: 1457 3309
BsaBI GATnn'nnATC
Cuts at: 6 1103 1145 2280 4317 6
Size: 1097 42 1135 2037 455
Fragments arranged by size:
2037 1135 1097 455 42
BsaHI Gr'CG_yC
Cuts at: 2197 2445 3147 4643 2197
Size: 248 702 1496 2320
Fragments arranged by size:
2320 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1955 2476 3154 3428 3575 3986 1955
Size: 521 678 274 147 411 2735
Fragments arranged by size:
2735 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2191 2949 3526 4022 2191
Size: 758 577 496 2935
Fragments arranged by size:
2935 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2308 2354 3562 104
Size: 2204 46 1208 1308
Fragments arranged by size:
2204 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1780 2558 2748 3540 1780
Size: 778 190 792 3006
Fragments arranged by size:
3006 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1477 1780 2391 2484 2558 2748 2810 3540 1477
Size: 303 611 93 74 190 62 730 2703
Fragments arranged by size:
2703 730 611 303 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3986 3986
Size: 4766
BspHI T'CATG_A
Cuts at: 3942 3942
Size: 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 118 1816 2030 2332 2713 118
Size: 1698 214 302 381 2171
Fragments arranged by size:
2171 1698 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3058 3112 4596 4761 50
Size: 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2678 2678
Size: 4766
BsrFI r'CCGG_y
Cuts at: 2764 2945 3154 2764
Size: 181 209 4376
Fragments arranged by size:
4376 209 181
BssSI C'ACGA_G
Cuts at: 3037 3395 3037
Size: 358 4408
BstAPI GCAn_nnn'nTGC
Cuts at: 301 2143 301
Size: 1842 2924
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstYI r'GATC_y
Cuts at: 1 829 1347 1791 2258 2281 2616 2862 3874
Size: 828 518 444 467 23 335 246 1012
Cuts at: 3874 3960 3972 4318 1
Size: 86 12 346 449
Fragments arranged by size:
1012 828 518 467 449 444 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 878 878
Size: 4766
Bsu36I CC'TnA_GG
Cuts at: 1676 1676
Size: 4766
BtgI C'CryG_G
Cuts at: 107 398 107
Size: 291 4475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 1143 1565 2009 1143
Size: 422 444 3900
Fragments arranged by size:
3900 444 422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1292 1327 1292
Size: 35 4731
DraI TTT'AAA
Cuts at: 909 1765 909
Size: 856 3910
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3330 2472
Size: 858 3908
EaeI y'GGCC_r
Cuts at: 575 942 2351 2525 2916 2943 4288 575
Size: 367 1409 174 391 27 1345 1053
Fragments arranged by size:
1409 1345 1053 391 367 174 27
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 249 1245 1652 2789 2999 249
Size: 996 407 1137 210 2016
Fragments arranged by size:
2016 1137 996 407 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1036 2974 3284 3430 4576 1036
Size: 1938 310 146 1146 1226
Fragments arranged by size:
1938 1226 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1778 1778
Size: 4766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 580 740 2591 3023 3770 4038 368
Size: 212 160 1851 432 747 268 1096
Fragments arranged by size:
1851 1096 747 432 268 212 160
EcoNI CCTnn'n_nnAGG
Cuts at: 203 203
Size: 4766
EcoO109I rG'GnC_Cy
Cuts at: 208 1223 1709 1905 208
Size: 1015 486 196 3069
Fragments arranged by size:
3069 1015 486 196
EcoRI G'AATT_C
Cuts at: 378 1207 1770 378
Size: 829 563 3374
Fragments arranged by size:
3374 829 563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 698 2785 698
Size: 2087 2679
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 2200 2448 3470 4449 2200
Size: 248 1022 979 2517
Fragments arranged by size:
2517 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 622 654 2608 2638 2640 2670 622
Size: 32 1954 30 2 30 2718
Fragments arranged by size:
2718 1954 32 30 30 2
HincII GTy'rAC
Cuts at: 1805 1805
Size: 4766
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MfeI C'AATT_G
Cuts at: 571 571
Size: 4766
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3437 3621 3437
Size: 184 4582
MscI TGG'CCA
Cuts at: 944 2527 944
Size: 1583 3183
MslI CAynn'nnrTG
Cuts at: 1076 1201 1559 1757 2755 2882 1076
Size: 125 358 198 998 127 2960
Fragments arranged by size:
2960 998 358 198 127 125
MspA1I CmG'CkG
Cuts at: 1877 2191 2551 3564 3809 1877
Size: 314 360 1013 245 2834
Fragments arranged by size:
2834 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NcoI C'CATG_G
Cuts at: 107 398 107
Size: 291 4475
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NspI r_CATG'y
Cuts at: 1760 1819 2319 3226 1760
Size: 59 500 907 3300
Fragments arranged by size:
3300 907 500 59
PciI A'CATG_T
Cuts at: 1756 3222 1756
Size: 1466 3300
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4734
PpuMI rG'GwC_Cy
Cuts at: 208 1223 1709 208
Size: 1015 486 3265
Fragments arranged by size:
3265 1015 486
PsiI TTA'TAA
Cuts at: 244 506 244
Size: 262 4504
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1052 1084 1052
Size: 32 4734
PstI C_TGCA'G
Cuts at: 1813 2498 1813
Size: 685 4081
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 2191 2551 2191
Size: 360 4406
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 1780 1780
Size: 4766
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 179 4283 179
Size: 4104 662
SfcI C'TryA_G
Cuts at: 33 1651 1809 2494 3487 3678 33
Size: 1618 158 685 993 191 1121
Fragments arranged by size:
1618 1121 993 685 191 158
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1788 1788
Size: 4766
SmlI C'TyrA_G
Cuts at: 1129 1351 1921 2836 3328 3590 1129
Size: 222 570 915 492 262 2305
Fragments arranged by size:
2305 915 570 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
SspI AAT'ATT
Cuts at: 1065 1065
Size: 4766
StuI AGG'CCT
Cuts at: 416 416
Size: 4766
StyI C'CwwG_G
Cuts at: 107 398 1226 1900 2254 107
Size: 291 828 674 354 2619
Fragments arranged by size:
2619 828 674 354 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 855 2349 2482 2941 3150 855
Size: 1494 133 459 209 2471
Fragments arranged by size:
2471 1494 459 209 133
TatI w'GTAC_w
Cuts at: 177 4281 177
Size: 4104 662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1496 1528 4283 4315 1496
Size: 32 2755 32 1947
Fragments arranged by size:
2755 1947 32 32
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2261 2261
Size: 4766
XmaI C'CCGG_G
Cuts at: 1786 1786
Size: 4766
XmnI GAAnn'nnTTC
Cuts at: 497 1550 497
Size: 1053 3713
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI ClaI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI