Restriction map for
pF1KE2335
(Circular) (Six-base) MAPSORT of: pF1KE2335.seq Check: 7537 from: 1 to: 7553
pF1KE2335 7553 bp
With 182 enzymes: SgfI *
May 11, 2017 11:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3004 3004
Size: 7553
AccI GT'mk_AC
Cuts at: 1793 2910 4591 1793
Size: 1117 1681 4755
Fragments arranged by size:
4755 1681 1117
Acc65I G'GTAC_C
Cuts at: 4569 4569
Size: 7553
AclI AA'CG_TT
Cuts at: 7393 7393
Size: 7553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1380 1523 2374 2886 3263 3917 5378 5810 6557
Size: 143 851 512 377 654 1461 432 747
Cuts at: 6557 6825 1380
Size: 268 2108
Fragments arranged by size:
2108 1461 851 747 654 512 432 377
268 143
AflII C'TTAA_G
Cuts at: 969 969
Size: 7553
AflIII A'CryG_T
Cuts at: 3002 3696 4905 6009 6986 3002
Size: 694 1209 1104 977 3569
Fragments arranged by size:
3569 1209 1104 977 694
AgeI A'CCGG_T
Cuts at: 5941 5941
Size: 7553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1159 1159
Size: 7553
AlwNI CAG_nnn'CTG
Cuts at: 201 2332 4395 4858 6425 201
Size: 2131 2063 463 1567 1329
Fragments arranged by size:
2131 2063 1567 1329 463
ApaLI G'TGCA_C
Cuts at: 6323 6323
Size: 7553
ApoI r'AATT_y
Cuts at: 676 1568 1772 2082 2202 2236 2945 3354 3690
Size: 892 204 310 120 34 709 409 336
Cuts at: 3690 4557 6906 7162 676
Size: 867 2349 256 1067
Fragments arranged by size:
2349 1067 892 867 709 409 336 310
256 204 120 34
AseI AT'TA_AT
Cuts at: 20 317 1439 1957 3103 20
Size: 297 1122 518 1146 4470
Fragments arranged by size:
4470 1146 1122 518 297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7553
AvaI C'yCGr_G
Cuts at: 4573 4573
Size: 7553
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1409 1442 1409
Size: 33 7520
BamHI G'GATC_C
Cuts at: 1480 4020 4236 4578 1480
Size: 2540 216 342 4455
Fragments arranged by size:
4455 2540 342 216
BanI G'GyrC_C
Cuts at: 1606 4569 4983 5231 5266 1606
Size: 2963 414 248 35 3893
Fragments arranged by size:
3893 2963 414 248 35
BanII G_rGCy'C
Cuts at: 4567 5597 4567
Size: 1030 6523
BbeI G_GCGC'C
Cuts at: 4987 5235 4987
Size: 248 7305
BbsI GAAGACnn'nnnn_
Cuts at: 601 2594 601
Size: 1993 5560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 4188 4222 90
Size: 34 4064 34 3421
Fragments arranged by size:
4064 3421 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 181 1828 5451 6212 7553 181
Size: 1647 3623 761 1341 181
Fragments arranged by size:
3623 1647 1341 761 181
BclI T'GATC_A
Cuts at: 2369 4041 5073 2369
Size: 1672 1032 4849
Fragments arranged by size:
4849 1672 1032
BglI GCCn_nnn'nGGC
Cuts at: 2503 2503
Size: 7553
BglII A'GATC_T
Cuts at: 3747 4081 4180 4202 5068 7105 3747
Size: 334 99 22 866 2037 4195
Fragments arranged by size:
4195 2037 866 334 99 22
BlpI GC'TnA_GC
Cuts at: 1029 4665 1029
Size: 3636 3917
Bme1580I G_kGCm'C
Cuts at: 5178 5271 6327 5178
Size: 93 1056 6404
Fragments arranged by size:
6404 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 421 4217 4765 4846 5181 7325 421
Size: 3796 548 81 335 2144 649
Fragments arranged by size:
3796 2144 649 548 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 137 474 1713 5920 137
Size: 337 1239 4207 1770
Fragments arranged by size:
4207 1770 1239 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 1508 1552 2198 3537 363
Size: 1145 44 646 1339 4379
Fragments arranged by size:
4379 1339 1145 646 44
Bpu10I CC'TnA_GC
Cuts at: 4144 4144
Size: 7553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 4455 4729 5608 6100 6398 2531
Size: 1924 274 879 492 298 3686
Fragments arranged by size:
3686 1924 879 492 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 2092 4081 7257 2092
Size: 1989 3176 2388
Fragments arranged by size:
3176 2388 1989
BsaAI yAC'GTr
Cuts at: 2865 3699 4906 2865
Size: 834 1207 5512
Fragments arranged by size:
5512 1207 834
BsaBI GATnn'nnATC
Cuts at: 6 5067 7104 6
Size: 5061 2037 455
Fragments arranged by size:
5061 2037 455
BsaHI Gr'CG_yC
Cuts at: 769 4984 5232 5934 7430 769
Size: 4215 248 702 1496 892
Fragments arranged by size:
4215 1496 892 702 248
BsaWI w'CCGG_w
Cuts at: 1106 4463 4742 5263 5941 6215 6362 6773 1106
Size: 3357 279 521 678 274 147 411 1886
Fragments arranged by size:
3357 1886 678 521 411 279 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 1327 2759 4422 4528 727
Size: 600 1432 1663 106 3752
Fragments arranged by size:
3752 1663 1432 600 106
BseYI C'CCAG_C
Cuts at: 525 820 862 935 4978 5736 6313 6809 525
Size: 295 42 73 4043 758 577 496 1269
Fragments arranged by size:
4043 1269 758 577 496 295 73 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2090
Size: 7553
BsiEI CG_ry'CG
Cuts at: 104 5095 5141 6349 104
Size: 4991 46 1208 1308
Fragments arranged by size:
4991 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4230 4567 5345 5535 6327 4230
Size: 337 778 190 792 5456
Fragments arranged by size:
5456 792 778 337 190
BsmI GAATG_Cn'
Cuts at: 560 4339 560
Size: 3779 3774
Bsp1286I G_dGCh'C
Cuts at: 4230 4567 5178 5271 5345 5535 5597 6327 4230
Size: 337 611 93 74 190 62 730 5456
Fragments arranged by size:
5456 730 611 337 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1106 6773 1106
Size: 5667 1886
BspHI T'CATG_A
Cuts at: 3524 6729 3524
Size: 3205 4348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3004 4603 4817 5119 5500 3004
Size: 1599 214 302 381 5057
Fragments arranged by size:
5057 1599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5845 5899 7383 7548 50
Size: 5795 54 1484 165 55
Fragments arranged by size:
5795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 953 3440 3532 4331 5465 953
Size: 2487 92 799 1134 3041
Fragments arranged by size:
3041 2487 1134 799 92
BsrFI r'CCGG_y
Cuts at: 5551 5732 5941 5551
Size: 181 209 7163
Fragments arranged by size:
7163 209 181
BsrGI T'GTAC_A
Cuts at: 1179 1179
Size: 7553
BssSI C'ACGA_G
Cuts at: 5824 6182 5824
Size: 358 7195
BstAPI GCAn_nnn'nTGC
Cuts at: 4930 4930
Size: 7553
BstBI TT'CG_AA
Cuts at: 5914 5914
Size: 7553
BstXI CCAn_nnnn'nTGG
Cuts at: 3916 3916
Size: 7553
BstYI r'GATC_y
Cuts at: 1 1480 3747 4020 4081 4180 4202 4236 4578
Size: 1479 2267 273 61 99 22 34 342
Cuts at: 4578 5045 5068 5403 5649 6661 6747 6759 7105
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7105 1
Size: 449
Fragments arranged by size:
2267 1479 1012 467 449 346 342 335
273 246 99 86 61 34 23 22
12
BstZ17I GTA'TAC
Cuts at: 1794 1794
Size: 7553
Bsu36I CC'TnA_GG
Cuts at: 2501 3723 2501
Size: 1222 6331
BtgI C'CryG_G
Cuts at: 4149 4149
Size: 7553
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 345 5503 5684 5847 345
Size: 5158 181 163 2051
Fragments arranged by size:
5158 2051 181 163
BtsI GCAGTG_nn'
Cuts at: 511 2639 2825 3391 3544 4796 511
Size: 2128 186 566 153 1252 3268
Fragments arranged by size:
3268 2128 1252 566 186 153
ClaI AT'CG_AT
Cuts at: 3914 3914
Size: 7553
DraI TTT'AAA
Cuts at: 602 1937 2169 4552 602
Size: 1335 232 2383 3603
Fragments arranged by size:
3603 2383 1335 232
DraIII CAC_nnn'GTG
Cuts at: 1753 3304 1753
Size: 1551 6002
DrdI GACnn_nn'nnGTC
Cuts at: 5259 6117 5259
Size: 858 6695
EaeI y'GGCC_r
Cuts at: 3660 3816 4152 5138 5312 5703 5730 7075 3660
Size: 156 336 986 174 391 27 1345 4138
Fragments arranged by size:
4138 1345 986 391 336 174 156 27
EagI C'GGCC_G
Cuts at: 5138 5138
Size: 7553
EarI CTCTTCn'nnn_
Cuts at: 1396 1682 2276 2777 3483 3850 4352 4420 4520
Size: 286 594 501 706 367 502 68 100
Cuts at: 4520 5576 5786 1396
Size: 1056 210 3163
Fragments arranged by size:
3163 1056 706 594 502 501 367 286
210 100 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5761 6071 6217 7363 5761
Size: 310 146 1146 5951
Fragments arranged by size:
5951 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4565 4565
Size: 7553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 1380 1508 1523 1552 2198 2374 2886 3263
Size: 1017 128 15 29 646 176 512 377
Cuts at: 3263 3537 3917 5378 5810 6557 6825 363
Size: 274 380 1461 432 747 268 1091
Fragments arranged by size:
1461 1091 1017 747 646 512 432 380
377 274 268 176 128 29 15
EcoNI CCTnn'n_nnAGG
Cuts at: 814 814
Size: 7553
EcoO109I rG'GnC_Cy
Cuts at: 4692 4692
Size: 7553
EcoRI G'AATT_C
Cuts at: 2202 3690 4557 2202
Size: 1488 867 5198
Fragments arranged by size:
5198 1488 867
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1793 2277 2619 4131 5572 1793
Size: 484 342 1512 1441 3774
Fragments arranged by size:
3774 1512 1441 484 342
FspI TGC'GCA
Cuts at: 1147 5334 1147
Size: 4187 3366
HaeII r_GCGC'y
Cuts at: 201 4987 5235 6257 7236 201
Size: 4786 248 1022 979 518
Fragments arranged by size:
4786 1022 979 518 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 1743 1775 2014 2046 2340 2372 3907
Size: 32 1473 32 239 32 294 32 1535
Cuts at: 3907 3939 4172 4204 4488 4520 5395 5425 5427
Size: 32 233 32 284 32 875 30 2
Cuts at: 5427 5457 238
Size: 30 2334
Fragments arranged by size:
2334 1535 1473 875 294 284 239 233
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1762 2439 2993 3389 3641 4592 1762
Size: 677 554 396 252 951 4723
Fragments arranged by size:
4723 951 677 554 396 252
HindIII A'AGCT_T
Cuts at: 3279 4108 3279
Size: 829 6724
HpaI GTT'AAC
Cuts at: 2439 2439
Size: 7553
KasI G'GCGC_C
Cuts at: 4983 5231 4983
Size: 248 7305
KpnI G_GTAC'C
Cuts at: 4573 4573
Size: 7553
MluI A'CGCG_T
Cuts at: 6986 6986
Size: 7553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 686 4463 6224 6408 686
Size: 3777 1761 184 1831
Fragments arranged by size:
3777 1831 1761 184
MscI TGG'CCA
Cuts at: 3662 3818 4154 5314 3662
Size: 156 336 1160 5901
Fragments arranged by size:
5901 1160 336 156
MslI CAynn'nnrTG
Cuts at: 553 2365 2863 3537 3562 3610 5542 5669 553
Size: 1812 498 674 25 48 1932 127 2437
Fragments arranged by size:
2437 1932 1812 674 498 127 48 25
MspA1I CmG'CkG
Cuts at: 161 4664 4978 5338 6351 6596 161
Size: 4503 314 360 1013 245 1118
Fragments arranged by size:
4503 1118 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5734 5734
Size: 7553
NarI GG'CG_CC
Cuts at: 4984 5232 4984
Size: 248 7305
NcoI C'CATG_G
Cuts at: 4149 4149
Size: 7553
NgoMIV G'CCGG_C
Cuts at: 5732 5732
Size: 7553
NruI TCG'CGA
Cuts at: 466 466
Size: 7553
NspI r_CATG'y
Cuts at: 1679 3615 4606 5106 6013 1679
Size: 1936 991 500 907 3219
Fragments arranged by size:
3219 1936 991 907 500
PasI CC'CwG_GG
Cuts at: 1172 1172
Size: 7553
PciI A'CATG_T
Cuts at: 6009 6009
Size: 7553
PflMI CCAn_nnn'nTGG
Cuts at: 3825 3825
Size: 7553
PfoI T'CCnGG_A
Cuts at: 1591 2140 1591
Size: 549 7004
PmeI GTTT'AAAC
Cuts at: 4552 4552
Size: 7553
PmlI CAC'GTG
Cuts at: 3699 3699
Size: 7553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6692 6724 6692
Size: 32 7521
PsiI TTA'TAA
Cuts at: 590 590
Size: 7553
PstI C_TGCA'G
Cuts at: 4600 5285 4600
Size: 685 6868
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7553
PvuII CAG'CTG
Cuts at: 161 4978 5338 161
Size: 4817 360 2376
Fragments arranged by size:
4817 2376 360
RsrII CG'GwC_CG
Cuts at: 5748 5748
Size: 7553
SacI G_AGCT'C
Cuts at: 4567 4567
Size: 7553
SalI G'TCGA_C
Cuts at: 4590 4590
Size: 7553
SapI GCTCTTCn'nnn_
Cuts at: 5576 5786 5576
Size: 210 7343
SbfI CC_TGCA'GG
Cuts at: 4600 4600
Size: 7553
ScaI AGT'ACT
Cuts at: 2793 3861 7070 2793
Size: 1068 3209 3276
Fragments arranged by size:
3276 3209 1068
SfcI C'TryA_G
Cuts at: 33 1274 2014 3119 4596 5281 6274 6465 33
Size: 1241 740 1105 1477 685 993 191 1121
Fragments arranged by size:
1477 1241 1121 1105 993 740 685 191
SfoI GGC'GCC
Cuts at: 4985 5233 4985
Size: 248 7305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7553
SmaI CCC'GGG
Cuts at: 4575 4575
Size: 7553
SmlI C'TyrA_G
Cuts at: 969 2546 4470 4708 5623 6115 6377 969
Size: 1577 1924 238 915 492 262 2145
Fragments arranged by size:
2145 1924 1577 915 492 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7553
SphI G_CATG'C
Cuts at: 1679 4606 5106 1679
Size: 2927 500 4126
Fragments arranged by size:
4126 2927 500
SspI AAT'ATT
Cuts at: 1115 1865 1955 1115
Size: 750 90 6713
Fragments arranged by size:
6713 750 90
StyI C'CwwG_G
Cuts at: 2442 2757 4149 4687 5041 2442
Size: 315 1392 538 354 4954
Fragments arranged by size:
4954 1392 538 354 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 539 5136 5269 5728 5937 539
Size: 4597 133 459 209 2155
Fragments arranged by size:
4597 2155 459 209 133
TatI w'GTAC_w
Cuts at: 396 845 1179 2791 3529 3859 7068 396
Size: 449 334 1612 738 330 3209 881
Fragments arranged by size:
3209 1612 881 738 449 334 330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 858 890 3288 3320 7070 7102 858
Size: 32 2398 32 3750 32 1309
Fragments arranged by size:
3750 2398 1309 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4117 5350 4117
Size: 1233 6320
XbaI T'CTAG_A
Cuts at: 621 4584 621
Size: 3963 3590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3561 4230 5048 3561
Size: 669 818 6066
Fragments arranged by size:
6066 818 669
XmaI C'CCGG_G
Cuts at: 4573 4573
Size: 7553
XmnI GAAnn'nnTTC
Cuts at: 1114 1368 1569 2082 2238 4097 1114
Size: 254 201 513 156 1859 4570
Fragments arranged by size:
4570 1859 513 254 201 156
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BssHII
BstEII CspCI EcoRV FseI FspAI MfeI NdeI NheI
NotI NsiI PacI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI