Restriction map for
pF1KE2339
(Circular) (Six-base) MAPSORT of: pF1KE2339.seq Check: 9993 from: 1 to: 3983
pF1KE2339 3983 bp
With 182 enzymes: SgfI *
May 6, 2016 19:10 ..
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1808 2240 2987 3255 346
Size: 1462 432 747 268 1074
Fragments arranged by size:
1462 1074 747 432 268
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 688 2371 688
Size: 1683 2300
AlwNI CAG_nnn'CTG
Cuts at: 324 423 1288 2855 324
Size: 99 865 1567 1452
Fragments arranged by size:
1567 1452 865 99
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 192 1003 192
Size: 811 3172
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 631 999 1413 1661 1696 631
Size: 368 414 248 35 2918
Fragments arranged by size:
2918 414 368 248 35
BanII G_rGCy'C
Cuts at: 314 997 2027 314
Size: 683 1030 2270
Fragments arranged by size:
2270 1030 683
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbsI GAAGACnn'nnnn_
Cuts at: 334 334
Size: 3983
BbvCI CC'TCA_GC
Cuts at: 151 151
Size: 3983
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglI GCCn_nnn'nGGC
Cuts at: 355 355
Size: 3983
BglII A'GATC_T
Cuts at: 599 1498 3535 599
Size: 899 2037 1047
Fragments arranged by size:
2037 1047 899
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2757 1608
Size: 93 1056 2834
Fragments arranged by size:
2834 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 474 576 1195 1276 1611 3755 474
Size: 102 619 81 335 2144 702
Fragments arranged by size:
2144 702 619 335 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 429 416
Size: 13 3970
Bpu10I CC'TnA_GC
Cuts at: 151 151
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 180 713 1414 1662 2364 3860 180
Size: 533 701 248 702 1496 303
Fragments arranged by size:
1496 702 701 533 303 248
BsaWI w'CCGG_w
Cuts at: 688 695 920 1172 1693 2371 2645 2792 3203
Size: 7 225 252 521 678 274 147 411
Cuts at: 3203 688
Size: 1468
Fragments arranged by size:
1468 678 521 411 274 252 225 147
7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 427 457 427
Size: 30 3953
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 352
Size: 3983
BseYI C'CCAG_C
Cuts at: 1408 2166 2743 3239 1408
Size: 758 577 496 2152
Fragments arranged by size:
2152 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 803
Size: 3983
BsiEI CG_ry'CG
Cuts at: 104 350 1525 1571 2779 104
Size: 246 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 246 46
BsiHKAI G_wGCw'C
Cuts at: 314 997 1775 1965 2757 314
Size: 683 778 190 792 1540
Fragments arranged by size:
1540 792 778 683 190
BsmI GAATG_Cn'
Cuts at: 846 846
Size: 3983
Bsp1286I G_dGCh'C
Cuts at: 314 997 1608 1701 1775 1965 2027 2757 314
Size: 683 611 93 74 190 62 730 1540
Fragments arranged by size:
1540 730 683 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3203 3203
Size: 3983
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 1033 1247 1549 1930 715
Size: 318 214 302 381 2768
Fragments arranged by size:
2768 381 318 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 370 568 688 1981 2162 2371 370
Size: 198 120 1293 181 209 1982
Fragments arranged by size:
1982 1293 209 198 181 120
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstEII G'GTnAC_C
Cuts at: 691 691
Size: 3983
BstYI r'GATC_y
Cuts at: 1 599 698 1008 1475 1498 1833 2079 3091
Size: 598 99 310 467 23 335 246 1012
Cuts at: 3091 3177 3189 3535 1
Size: 86 12 346 449
Fragments arranged by size:
1012 598 467 449 346 335 310 246
99 86 23 12
BtgI C'CryG_G
Cuts at: 107 141 245 350 107
Size: 34 104 105 3740
Fragments arranged by size:
3740 105 104 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 201 308 483 685 835 1933 2114 2277 201
Size: 107 175 202 150 1098 181 163 1907
Fragments arranged by size:
1907 1098 202 181 175 163 150 107
BtsI GCAGTG_nn'
Cuts at: 1226 1226
Size: 3983
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 110 347 824 1568 1742 2133 2160 3505 110
Size: 237 477 744 174 391 27 1345 588
Fragments arranged by size:
1345 744 588 477 391 237 174 27
EagI C'GGCC_G
Cuts at: 347 1568 347
Size: 1221 2762
EarI CTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2501 2647 3793 2191
Size: 310 146 1146 2381
Fragments arranged by size:
2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 312 995 312
Size: 683 3300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 416 429 1808 2240 2987 3255 346
Size: 70 13 1379 432 747 268 1074
Fragments arranged by size:
1379 1074 747 432 268 70 13
EcoNI CCTnn'n_nnAGG
Cuts at: 478 478
Size: 3983
EcoO109I rG'GnC_Cy
Cuts at: 155 276 321 342 1122 155
Size: 121 45 21 780 3016
Fragments arranged by size:
3016 780 121 45 21
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
EcoRV GAT'ATC
Cuts at: 405 558 405
Size: 153 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 745 909 981 2002 745
Size: 164 72 1021 2726
Fragments arranged by size:
2726 1021 164 72
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 389 421 427 459 685 717 1825 1855 1857
Size: 32 6 32 226 32 1108 30 2
Cuts at: 1857 1887 389
Size: 30 2485
Fragments arranged by size:
2485 1108 226 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 282 860 2654 2838 282
Size: 578 1794 184 1427
Fragments arranged by size:
1794 1427 578 184
MscI TGG'CCA
Cuts at: 826 1744 826
Size: 918 3065
MslI CAynn'nnrTG
Cuts at: 1972 2099 1972
Size: 127 3856
MspA1I CmG'CkG
Cuts at: 143 150 394 1094 1408 1768 2781 3026 143
Size: 7 244 700 314 360 1013 245 1100
Fragments arranged by size:
1100 1013 700 360 314 245 244 7
NaeI GCC'GGC
Cuts at: 2164 2164
Size: 3983
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 2162 2162
Size: 3983
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PfoI T'CCnGG_A
Cuts at: 431 431
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PpuMI rG'GwC_Cy
Cuts at: 342 342
Size: 3983
PstI C_TGCA'G
Cuts at: 1030 1715 1030
Size: 685 3298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 394 1408 1768 394
Size: 1014 360 2609
Fragments arranged by size:
2609 1014 360
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 314 997 314
Size: 683 3300
SacII CC_GC'GG
Cuts at: 144 144
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SexAI A'CCwGG_T
Cuts at: 974 974
Size: 3983
SfcI C'TryA_G
Cuts at: 33 1026 1711 2704 2895 33
Size: 993 685 993 191 1121
Fragments arranged by size:
1121 993 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 355 355
Size: 3983
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SgrAI Cr'CCGG_yG
Cuts at: 370 688 370
Size: 318 3665
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StuI AGG'CCT
Cuts at: 385 385
Size: 3983
StyI C'CwwG_G
Cuts at: 107 272 1117 1471 107
Size: 165 845 354 2619
Fragments arranged by size:
2619 845 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 649 1566 1699 2158 2367 493
Size: 156 917 133 459 209 2109
Fragments arranged by size:
2109 917 459 209 156 133
TatI w'GTAC_w
Cuts at: 209 3498 209
Size: 3289 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
XmnI GAAnn'nnTTC
Cuts at: 331 331
Size: 3983
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SanDI SnaBI SrfI
SspI SwaI XhoI ZraI