Restriction map for
pF1KE2344
(Circular) (Six-base) MAPSORT of: pF1KE2344.seq Check: 2883 from: 1 to: 7121
pF1KE2344 7121 bp
With 182 enzymes: SgfI *
June 23, 2011 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1477 3983 1477
Size: 2506 4615
AatII G_ACGT'C
Cuts at: 664 664
Size: 7121
AccI GT'mk_AC
Cuts at: 3572 4159 3572
Size: 587 6534
Acc65I G'GTAC_C
Cuts at: 4137 4137
Size: 7121
AclI AA'CG_TT
Cuts at: 6961 6961
Size: 7121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1593 1603 2251 4946 5378 6125 6393 607
Size: 986 10 648 2695 432 747 268 1335
Fragments arranged by size:
2695 1335 986 747 648 432 268 10
AfeI AGC'GCT
Cuts at: 767 1552 1744 2339 767
Size: 785 192 595 5549
Fragments arranged by size:
5549 785 595 192
AflIII A'CryG_T
Cuts at: 4473 5577 6554 4473
Size: 1104 977 5040
Fragments arranged by size:
5040 1104 977
AgeI A'CCGG_T
Cuts at: 5509 5509
Size: 7121
AhdI GACnn_n'nnGTC
Cuts at: 426 504 660 426
Size: 78 156 6887
Fragments arranged by size:
6887 156 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 421 2500 421
Size: 2079 5042
AlwNI CAG_nnn'CTG
Cuts at: 1107 2538 2795 2850 4426 5993 1107
Size: 1431 257 55 1576 1567 2235
Fragments arranged by size:
2235 1576 1567 1431 257 55
ApaI G_GGCC'C
Cuts at: 145 230 287 319 938 969 2022 2744 2894
Size: 85 57 32 619 31 1053 722 150
Cuts at: 2894 3761 3780 3977 145
Size: 867 19 197 3289
Fragments arranged by size:
3289 1053 867 722 619 197 150 85
57 32 31 19
ApaLI G'TGCA_C
Cuts at: 442 538 5891 442
Size: 96 5353 1672
Fragments arranged by size:
5353 1672 96
ApoI r'AATT_y
Cuts at: 4125 6474 6730 4125
Size: 2349 256 4516
Fragments arranged by size:
4516 2349 256
AscI GG'CGCG_CC
Cuts at: 3591 3870 3591
Size: 279 6842
AseI AT'TA_AT
Cuts at: 20 20
Size: 7121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7121
AvaI C'yCGr_G
Cuts at: 137 279 345 854 1840 2057 2887 3304 3430
Size: 142 66 509 986 217 830 417 126
Cuts at: 3430 3541 3580 3922 4102 4141 137
Size: 111 39 342 180 39 3117
Fragments arranged by size:
3117 986 830 509 417 342 217 180
142 126 111 66 39 39
AvrII C'CTAG_G
Cuts at: 3024 3024
Size: 7121
BamHI G'GATC_C
Cuts at: 4146 4146
Size: 7121
BanI G'GyrC_C
Cuts at: 115 201 547 997 1096 1465 2040 2720 2936
Size: 86 346 450 99 369 575 680 216
Cuts at: 2936 3054 3074 3172 3276 3729 3788 3863 4069
Size: 118 20 98 104 453 59 75 206
Cuts at: 4069 4137 4551 4799 4834 115
Size: 68 414 248 35 2402
Fragments arranged by size:
2402 680 575 453 450 414 369 346
248 216 206 118 104 99 98 86
75 68 59 35 20
BanII G_rGCy'C
Cuts at: 145 230 287 319 583 604 872 938 961
Size: 85 57 32 264 21 268 66 23
Cuts at: 961 969 980 1139 1847 2022 2186 2497 2744
Size: 8 11 159 708 175 164 311 247
Cuts at: 2744 2894 3164 3271 3375 3500 3761 3780 3977
Size: 150 270 107 104 125 261 19 197
Cuts at: 3977 4084 4135 5165 145
Size: 107 51 1030 2101
Fragments arranged by size:
2101 1030 708 311 270 268 264 261
247 197 175 164 159 150 125 107
107 104 85 66 57 51 32 23
21 19 11 8
BbeI G_GCGC'C
Cuts at: 119 205 551 1001 2724 2940 3078 3176 3280
Size: 86 346 450 1723 216 138 98 104
Cuts at: 3280 3733 4555 4803 119
Size: 453 822 248 2437
Fragments arranged by size:
2437 1723 822 453 450 346 248 216
138 104 98 86
BbsI GAAGACnn'nnnn_
Cuts at: 447 467 2448 2643 2685 447
Size: 20 1981 195 42 4883
Fragments arranged by size:
4883 1981 195 42 20
BbvCI CC'TCA_GC
Cuts at: 887 3931 887
Size: 3044 4077
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1013 1047 2439 2473 90
Size: 34 889 34 1392 34 4738
Fragments arranged by size:
4738 1392 889 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2163 5019 5780 7121 2163
Size: 2856 761 1341 2163
Fragments arranged by size:
2856 2163 1341 761
BclI T'GATC_A
Cuts at: 4641 4641
Size: 7121
BglI GCCn_nnn'nGGC
Cuts at: 131 140 304 328 1014 1087 2137 3217 3473
Size: 9 164 24 686 73 1050 1080 256
Cuts at: 3473 3716 131
Size: 243 3536
Fragments arranged by size:
3536 1080 1050 686 256 243 164 73
24 9
BglII A'GATC_T
Cuts at: 4636 6673 4636
Size: 2037 5084
BlpI GC'TnA_GC
Cuts at: 3098 4233 3098
Size: 1135 5986
Bme1580I G_kGCm'C
Cuts at: 145 230 287 319 446 542 690 810 938
Size: 85 57 32 127 96 148 120 128
Cuts at: 938 969 1101 2022 2045 2249 2744 2894 2909
Size: 31 132 921 23 204 495 150 15
Cuts at: 2909 3352 3418 3761 3780 3793 3943 3977 4072
Size: 443 66 343 19 13 150 34 95
Cuts at: 4072 4746 4839 5895 145
Size: 674 93 1056 1371
Fragments arranged by size:
1371 1056 921 674 495 443 343 204
150 150 148 132 128 127 120 96
95 93 85 66 57 34 32 31
23 19 15 13
BmgBI CAC'GTC
Cuts at: 357 1298 357
Size: 941 6180
BmrI ACTGGGnnnn_n'
Cuts at: 4023 4333 4414 4749 6893 4023
Size: 310 81 335 2144 4251
Fragments arranged by size:
4251 2144 335 310 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 597 1761 2937 5488 597
Size: 1164 1176 2551 2230
Fragments arranged by size:
2551 2230 1176 1164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 968 1438 2584 2760 3514 3685 664
Size: 304 470 1146 176 754 171 4100
Fragments arranged by size:
4100 1146 754 470 304 176 171
Bpu10I CC'TnA_GC
Cuts at: 887 1133 3931 887
Size: 246 2798 4077
Fragments arranged by size:
4077 2798 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2335 4297 5176 5668 5966 1324
Size: 1011 1962 879 492 298 2479
Fragments arranged by size:
2479 1962 1011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1774 6825 1774
Size: 5051 2070
BsaAI yAC'GTr
Cuts at: 1211 2166 4474 1211
Size: 955 2308 3858
Fragments arranged by size:
3858 2308 955
BsaBI GATnn'nnATC
Cuts at: 6 4635 6672 6
Size: 4629 2037 455
Fragments arranged by size:
4629 2037 455
BsaHI Gr'CG_yC
Cuts at: 116 202 548 661 998 1664 2369 2648 2721
Size: 86 346 113 337 666 705 279 73
Cuts at: 2721 2937 3075 3173 3277 3730 3771 4552 4800
Size: 216 138 98 104 453 41 781 248
Cuts at: 4800 5502 6998 116
Size: 702 1496 239
Fragments arranged by size:
1496 781 705 702 666 453 346 337
279 248 239 216 138 113 104 98
86 73 41
BsaWI w'CCGG_w
Cuts at: 1527 4043 4310 4831 5509 5783 5930 6341 1527
Size: 2516 267 521 678 274 147 411 2307
Fragments arranged by size:
2516 2307 678 521 411 274 267 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2479 2509 2557 2587 3487 3517 2479
Size: 30 48 30 900 30 6083
Fragments arranged by size:
6083 900 48 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 661 874 904 1753 2173 3383 211
Size: 450 213 30 849 420 1210 3949
Fragments arranged by size:
3949 1210 849 450 420 213 30
BseYI C'CCAG_C
Cuts at: 597 749 809 938 1316 2854 3321 3483 3602
Size: 152 60 129 378 1538 467 162 119
Cuts at: 3602 3877 3884 4546 5304 5881 6377 597
Size: 275 7 662 758 577 496 1341
Fragments arranged by size:
1538 1341 758 662 577 496 467 378
275 162 152 129 119 60 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 2470
Size: 7121
BsiEI CG_ry'CG
Cuts at: 104 256 299 323 2881 3095 3380 3558 3572
Size: 152 43 24 2558 214 285 178 14
Cuts at: 3572 3635 3799 4663 4709 5917 104
Size: 63 164 864 46 1208 1308
Fragments arranged by size:
2558 1308 1208 864 285 214 178 164
152 63 46 43 24 14
BsiHKAI G_wGCw'C
Cuts at: 380 446 542 563 758 803 1139 2396 2708
Size: 66 96 21 195 45 336 1257 312
Cuts at: 2708 4135 4913 5103 5895 380
Size: 1427 778 190 792 1606
Fragments arranged by size:
1606 1427 1257 792 778 336 312 195
190 96 66 45 21
BsiWI C'GTAC_G
Cuts at: 290 3138 290
Size: 2848 4273
Bsp1286I G_dGCh'C
Cuts at: 145 230 287 319 380 446 542 563 583
Size: 85 57 32 61 66 96 21 20
Cuts at: 583 604 690 758 803 810 872 938 961
Size: 21 86 68 45 7 62 66 23
Cuts at: 961 969 980 1101 1139 1847 2022 2045 2186
Size: 8 11 121 38 708 175 23 141
Cuts at: 2186 2249 2396 2497 2708 2744 2894 2909 3164
Size: 63 147 101 211 36 150 15 255
Cuts at: 3164 3271 3352 3375 3418 3500 3761 3780 3793
Size: 107 81 23 43 82 261 19 13
Cuts at: 3793 3943 3977 4072 4084 4135 4746 4839 4913
Size: 150 34 95 12 51 611 93 74
Cuts at: 4913 5103 5165 5895 145
Size: 190 62 730 1371
Fragments arranged by size:
1371 730 708 611 261 255 211 190
175 150 150 147 141 121 107 101
96 95 93 86 85 82 81 74
68 66 66 63 62 62 61 57
51 45 43 38 36 34 32 23
23 23 21 21 20 19 15 13
12 11 8 7
BspEI T'CCGG_A
Cuts at: 6341 6341
Size: 7121
BspHI T'CATG_A
Cuts at: 1199 2147 6297 1199
Size: 948 4150 2023
Fragments arranged by size:
4150 2023 948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 1477 3620 3983 4171 4385 4687 5068 431
Size: 1046 2143 363 188 214 302 381 2484
Fragments arranged by size:
2484 2143 1046 381 363 302 214 188
BsrBI CCG'CTC
Cuts at: 50 258 2568 3097 3382 5413 5467 6951 7116
Size: 208 2310 529 285 2031 54 1484 165
Cuts at: 7116 50
Size: 55
Fragments arranged by size:
2310 2031 1484 529 285 208 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1818 5033 1818
Size: 3215 3906
BsrFI r'CCGG_y
Cuts at: 118 220 1783 2880 3090 3169 3273 3866 5119
Size: 102 1563 1097 210 79 104 593 1253
Cuts at: 5119 5300 5509 118
Size: 181 209 1730
Fragments arranged by size:
1730 1563 1253 1097 593 210 209 181
104 102 79
BsrGI T'GTAC_A
Cuts at: 2786 2786
Size: 7121
BssHII G'CGCG_C
Cuts at: 471 473 827 1701 1804 2916 3046 3048 3591
Size: 2 354 874 103 1112 130 2 543
Cuts at: 3591 3870 3958 471
Size: 279 88 3634
Fragments arranged by size:
3634 1112 874 543 354 279 130 103
88 2 2
BssSI C'ACGA_G
Cuts at: 271 403 556 683 3936 5392 5750 271
Size: 132 153 127 3253 1456 358 1642
Fragments arranged by size:
3253 1642 1456 358 153 132 127
BstAPI GCAn_nnn'nTGC
Cuts at: 4498 4498
Size: 7121
BstBI TT'CG_AA
Cuts at: 458 5482 458
Size: 5024 2097
BstEII G'GTnAC_C
Cuts at: 1629 1629
Size: 7121
BstXI CCAn_nnnn'nTGG
Cuts at: 946 2520 946
Size: 1574 5547
BstYI r'GATC_y
Cuts at: 1 833 907 2585 4146 4613 4636 4971 5217
Size: 832 74 1678 1561 467 23 335 246
Cuts at: 5217 6229 6315 6327 6673 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1678 1561 1012 832 467 449 346 335
246 86 74 23 12
BtgI C'CryG_G
Cuts at: 129 323 365 759 1173 1958 2045 2258 2672
Size: 194 42 394 414 785 87 213 414
Cuts at: 2672 2875 3058 3215 3793 3814 129
Size: 203 183 157 578 21 3436
Fragments arranged by size:
3436 785 578 414 414 394 213 203
194 183 157 87 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2463 5071 5252 5415 2463
Size: 2608 181 163 4169
Fragments arranged by size:
4169 2608 181 163
BtsI GCAGTG_nn'
Cuts at: 825 1031 2396 2730 3651 4364 825
Size: 206 1365 334 921 713 3582
Fragments arranged by size:
3582 1365 921 713 334 206
ClaI AT'CG_AT
Cuts at: 1658 1658
Size: 7121
DraI TTT'AAA
Cuts at: 4120 4120
Size: 7121
DraIII CAC_nnn'GTG
Cuts at: 450 2712 450
Size: 2262 4859
DrdI GACnn_nn'nnGTC
Cuts at: 1765 4827 5685 1765
Size: 3062 858 3201
Fragments arranged by size:
3201 3062 858
EaeI y'GGCC_r
Cuts at: 253 296 320 533 693 1079 1088 1140 1409
Size: 43 24 213 160 386 9 52 269
Cuts at: 1409 1712 1955 2138 2669 2878 2978 3092 3218
Size: 303 243 183 531 209 100 114 126
Cuts at: 3218 3289 3377 3555 3632 4706 4880 5271 5298
Size: 71 88 178 77 1074 174 391 27
Cuts at: 5298 6643 253
Size: 1345 731
Fragments arranged by size:
1345 1074 731 531 391 386 303 269
243 213 209 183 178 174 160 126
114 100 88 77 71 52 43 27
24 9
EagI C'GGCC_G
Cuts at: 253 296 320 2878 3092 3377 3555 3632 4706
Size: 43 24 2558 214 285 178 77 1074
Cuts at: 4706 253
Size: 2668
Fragments arranged by size:
2668 2558 1074 285 214 178 77 43
24
EarI CTCTTCn'nnn_
Cuts at: 609 850 989 5144 5354 609
Size: 241 139 4155 210 2376
Fragments arranged by size:
4155 2376 241 210 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 826 1324 3374 3380 5329 5639 5785 6931 826
Size: 498 2050 6 1949 310 146 1146 1016
Fragments arranged by size:
2050 1949 1146 1016 498 310 146 6
EcoICRI GAG'CTC
Cuts at: 1137 4133 1137
Size: 2996 4125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 664 968 1438 1593 1603 2251 2584 2760
Size: 57 304 470 155 10 648 333 176
Cuts at: 2760 3514 3685 4946 5378 6125 6393 607
Size: 754 171 1261 432 747 268 1335
Fragments arranged by size:
1335 1261 754 747 648 470 432 333
304 268 176 171 155 57 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2488 2775 2488
Size: 287 6834
EcoO109I rG'GnC_Cy
Cuts at: 141 142 283 934 935 965 966 1104 2019
Size: 1 141 651 1 30 1 138 915
Cuts at: 2019 2207 2740 3021 3758 3888 3927 3974 4062
Size: 188 533 281 737 130 39 47 88
Cuts at: 4062 4260 141
Size: 198 3002
Fragments arranged by size:
3002 915 737 651 533 281 198 188
141 138 130 88 47 39 30 1
1 1
EcoRI G'AATT_C
Cuts at: 4125 4125
Size: 7121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5140 5140
Size: 7121
FspI TGC'GCA
Cuts at: 340 4902 340
Size: 4562 2559
HaeII r_GCGC'y
Cuts at: 119 172 205 271 373 551 769 1001 1554
Size: 53 33 66 102 178 218 232 553
Cuts at: 1554 1746 1971 2341 2724 2804 2940 3078 3176
Size: 192 225 370 383 80 136 138 98
Cuts at: 3176 3280 3540 3733 4555 4803 5825 6804 119
Size: 104 260 193 822 248 1022 979 436
Fragments arranged by size:
1022 979 822 553 436 383 370 260
248 232 225 218 193 192 178 138
136 104 102 98 80 66 53 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 412 444 490 522 646 678 1469 1501 2161
Size: 32 46 32 124 32 791 32 660
Cuts at: 2161 2193 3919 3951 4963 4993 4995 5025 412
Size: 32 1726 32 1012 30 2 30 2508
Fragments arranged by size:
2508 1726 1012 791 660 124 46 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3573 4160 3573
Size: 587 6534
HindIII A'AGCT_T
Cuts at: 2428 2428
Size: 7121
KasI G'GCGC_C
Cuts at: 115 201 547 997 2720 2936 3074 3172 3276
Size: 86 346 450 1723 216 138 98 104
Cuts at: 3276 3729 4551 4799 115
Size: 453 822 248 2437
Fragments arranged by size:
2437 1723 822 453 450 346 248 216
138 104 98 86
KpnI G_GTAC'C
Cuts at: 4141 4141
Size: 7121
MluI A'CGCG_T
Cuts at: 6554 6554
Size: 7121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3425 5792 5976 3425
Size: 2367 184 4570
Fragments arranged by size:
4570 2367 184
MscI TGG'CCA
Cuts at: 695 1090 1411 1714 1957 2671 4882 695
Size: 395 321 303 243 714 2211 2934
Fragments arranged by size:
2934 2211 714 395 321 303 243
MslI CAynn'nnrTG
Cuts at: 721 5110 5237 721
Size: 4389 127 2605
Fragments arranged by size:
4389 2605 127
MspA1I CmG'CkG
Cuts at: 131 597 761 886 942 1072 1175 1316 1350
Size: 466 164 125 56 130 103 141 34
Cuts at: 1350 1463 2501 2792 2877 2933 3060 3081 3217
Size: 113 1038 291 85 56 127 21 136
Cuts at: 3217 3626 3695 3782 3795 3816 3877 4026 4232
Size: 409 69 87 13 21 61 149 206
Cuts at: 4232 4546 4906 5919 6164 131
Size: 314 360 1013 245 1088
Fragments arranged by size:
1088 1038 1013 466 409 360 314 291
245 206 164 149 141 136 130 127
125 113 103 87 85 69 61 56
56 34 21 21 13
NaeI GCC'GGC
Cuts at: 2882 3092 3171 3275 5302 2882
Size: 210 79 104 2027 4701
Fragments arranged by size:
4701 2027 210 104 79
NarI GG'CG_CC
Cuts at: 116 202 548 998 2721 2937 3075 3173 3277
Size: 86 346 450 1723 216 138 98 104
Cuts at: 3277 3730 4552 4800 116
Size: 453 822 248 2437
Fragments arranged by size:
2437 1723 822 453 450 346 248 216
138 104 98 86
NcoI C'CATG_G
Cuts at: 2672 2672
Size: 7121
NgoMIV G'CCGG_C
Cuts at: 2880 3090 3169 3273 5300 2880
Size: 210 79 104 2027 4701
Fragments arranged by size:
4701 2027 210 104 79
NotI GC'GGCC_GC
Cuts at: 253 253
Size: 7121
NspI r_CATG'y
Cuts at: 2295 2661 2787 4174 4674 5581 2295
Size: 366 126 1387 500 907 3835
Fragments arranged by size:
3835 1387 907 500 366 126
PasI CC'CwG_GG
Cuts at: 781 3746 781
Size: 2965 4156
PciI A'CATG_T
Cuts at: 5577 5577
Size: 7121
PflMI CCAn_nnn'nTGG
Cuts at: 2754 2754
Size: 7121
PfoI T'CCnGG_A
Cuts at: 191 2153 191
Size: 1962 5159
PmeI GTTT'AAAC
Cuts at: 4120 4120
Size: 7121
PmlI CAC'GTG
Cuts at: 1211 1211
Size: 7121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 859 891 6260 6292 859
Size: 32 5369 32 1688
Fragments arranged by size:
5369 1688 32 32
PpuMI rG'GwC_Cy
Cuts at: 3888 3927 4062 3888
Size: 39 135 6947
Fragments arranged by size:
6947 135 39
PshAI GACnn'nnGTC
Cuts at: 737 737
Size: 7121
PspOMI G'GGCC_C
Cuts at: 141 226 283 315 934 965 2018 2740 2890
Size: 85 57 32 619 31 1053 722 150
Cuts at: 2890 3757 3776 3973 141
Size: 867 19 197 3289
Fragments arranged by size:
3289 1053 867 722 619 197 150 85
57 32 31 19
PspXI vC'TCGA_Gb
Cuts at: 1840 3922 4102 1840
Size: 2082 180 4859
Fragments arranged by size:
4859 2082 180
PstI C_TGCA'G
Cuts at: 413 596 1379 1452 2391 2534 2800 4168 4853
Size: 183 783 73 939 143 266 1368 685
Cuts at: 4853 413
Size: 2681
Fragments arranged by size:
2681 1368 939 783 685 266 183 143
73
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7121
PvuII CAG'CTG
Cuts at: 597 886 942 1072 1316 2792 3695 4546 4906
Size: 289 56 130 244 1476 903 851 360
Cuts at: 4906 597
Size: 2812
Fragments arranged by size:
2812 1476 903 851 360 289 244 130
56
RsrII CG'GwC_CG
Cuts at: 3628 5316 3628
Size: 1688 5433
SacI G_AGCT'C
Cuts at: 1139 4135 1139
Size: 2996 4125
SacII CC_GC'GG
Cuts at: 132 762 1176 2878 3061 3218 3796 3817 132
Size: 630 414 1702 183 157 578 21 3436
Fragments arranged by size:
3436 1702 630 578 414 183 157 21
SalI G'TCGA_C
Cuts at: 3571 4158 3571
Size: 587 6534
SapI GCTCTTCn'nnn_
Cuts at: 609 850 5144 5354 609
Size: 241 4294 210 2376
Fragments arranged by size:
4294 2376 241 210
SbfI CC_TGCA'GG
Cuts at: 4168 4168
Size: 7121
ScaI AGT'ACT
Cuts at: 6638 6638
Size: 7121
SexAI A'CCwGG_T
Cuts at: 1625 2708 1625
Size: 1083 6038
SfcI C'TryA_G
Cuts at: 33 409 592 1375 1448 1848 2387 2530 2796
Size: 376 183 783 73 400 539 143 266
Cuts at: 2796 4164 4849 5842 6033 33
Size: 1368 685 993 191 1121
Fragments arranged by size:
1368 1121 993 783 685 539 400 376
266 191 183 143 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 131 140 1087 131
Size: 9 947 6165
Fragments arranged by size:
6165 947 9
SfoI GGC'GCC
Cuts at: 117 203 549 999 2722 2938 3076 3174 3278
Size: 86 346 450 1723 216 138 98 104
Cuts at: 3278 3731 4553 4801 117
Size: 453 822 248 2437
Fragments arranged by size:
2437 1723 822 453 450 346 248 216
138 104 98 86
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7121
SgrAI Cr'CCGG_yG
Cuts at: 118 3169 3273 3866 118
Size: 3051 104 593 3373
Fragments arranged by size:
3373 3051 593 104
SmaI CCC'GGG
Cuts at: 347 2059 2889 4143 347
Size: 1712 830 1254 3325
Fragments arranged by size:
3325 1712 1254 830
SmlI C'TyrA_G
Cuts at: 1339 1840 2350 3430 3922 4102 4276 5191 5683
Size: 501 510 1080 492 180 174 915 492
Cuts at: 5683 5945 1339
Size: 262 2515
Fragments arranged by size:
2515 1080 915 510 501 492 492 262
180 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7121
SphI G_CATG'C
Cuts at: 4174 4674 4174
Size: 500 6621
SrfI GCCC'GGGC
Cuts at: 347 347
Size: 7121
StuI AGG'CCT
Cuts at: 3029 3029
Size: 7121
StyI C'CwwG_G
Cuts at: 569 2672 2945 3024 4255 4609 569
Size: 2103 273 79 1231 354 3081
Fragments arranged by size:
3081 2103 1231 354 273 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1183 1854 2761 3558 4704 4837 5296 5505 1183
Size: 671 907 797 1146 133 459 209 2799
Fragments arranged by size:
2799 1146 907 797 671 459 209 133
TatI w'GTAC_w
Cuts at: 2714 2786 6636 2714
Size: 72 3850 3199
Fragments arranged by size:
3850 3199 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2479 2511 2557 2589 3487 3519 6638 6670 2479
Size: 32 46 32 898 32 3119 32 2930
Fragments arranged by size:
3119 2930 898 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1483 4918 1483
Size: 3435 3686
XbaI T'CTAG_A
Cuts at: 4152 4152
Size: 7121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 2751 4616 2076
Size: 675 1865 4581
Fragments arranged by size:
4581 1865 675
XhoI C'TCGA_G
Cuts at: 1840 3430 3922 4102 1840
Size: 1590 492 180 4859
Fragments arranged by size:
4859 1590 492 180
XmaI C'CCGG_G
Cuts at: 345 2057 2887 4141 345
Size: 1712 830 1254 3325
Fragments arranged by size:
3325 1712 1254 830
ZraI GAC'GTC
Cuts at: 662 662
Size: 7121
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AleI AloI BaeI BfrBI BmtI BsmI BsmBI
BstZ17I Bsu36I CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PsiI PsrI SanDI
SnaBI SspI SwaI XmnI