Restriction map for
pF1KE2347
(Circular) (Six-base) MAPSORT of: pF1KE2347.seq Check: 5562 from: 1 to: 5327
pF1KE2347 5327 bp
With 182 enzymes: SgfI *
June 23, 2011 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 398 398
Size: 5327
AccI GT'mk_AC
Cuts at: 806 2365 806
Size: 1559 3768
Acc65I G'GTAC_C
Cuts at: 2343 2343
Size: 5327
AclI AA'CG_TT
Cuts at: 5167 5167
Size: 5327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 1949 3152 3584 4331 4599 806
Size: 1143 1203 432 747 268 1534
Fragments arranged by size:
1534 1203 1143 747 432 268
AflIII A'CryG_T
Cuts at: 2679 3783 4760 2679
Size: 1104 977 3246
Fragments arranged by size:
3246 1104 977
AgeI A'CCGG_T
Cuts at: 3715 3715
Size: 5327
AhdI GACnn_n'nnGTC
Cuts at: 1826 1826
Size: 5327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 902 902
Size: 5327
AlwNI CAG_nnn'CTG
Cuts at: 481 943 967 1669 2632 4199 481
Size: 462 24 702 963 1567 1609
Fragments arranged by size:
1609 1567 963 702 462 24
ApaI G_GGCC'C
Cuts at: 275 1002 275
Size: 727 4600
ApaLI G'TGCA_C
Cuts at: 4097 4097
Size: 5327
ApoI r'AATT_y
Cuts at: 1959 2066 2331 4680 4936 1959
Size: 107 265 2349 256 2350
Fragments arranged by size:
2350 2349 265 256 107
AseI AT'TA_AT
Cuts at: 20 20
Size: 5327
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5327
AvaI C'yCGr_G
Cuts at: 142 217 462 529 1289 2347 142
Size: 75 245 67 760 1058 3122
Fragments arranged by size:
3122 1058 760 245 75 67
AvrII C'CTAG_G
Cuts at: 753 753
Size: 5327
BamHI G'GATC_C
Cuts at: 2352 2352
Size: 5327
BanI G'GyrC_C
Cuts at: 340 522 1071 1496 1626 2343 2757 3005 3040
Size: 182 549 425 130 717 414 248 35
Cuts at: 3040 340
Size: 2627
Fragments arranged by size:
2627 717 549 425 414 248 182 130
35
BanII G_rGCy'C
Cuts at: 157 275 390 761 989 1002 1319 1456 1511
Size: 118 115 371 228 13 317 137 55
Cuts at: 1511 1526 2341 3371 157
Size: 15 815 1030 2113
Fragments arranged by size:
2113 1030 815 371 317 228 137 118
115 55 15 13
BbeI G_GCGC'C
Cuts at: 344 526 1500 2761 3009 344
Size: 182 974 1261 248 2662
Fragments arranged by size:
2662 1261 974 248 182
BbvCI CC'TCA_GC
Cuts at: 1169 1729 1907 1169
Size: 560 178 4589
Fragments arranged by size:
4589 560 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1219 1253 90
Size: 34 1095 34 4164
Fragments arranged by size:
4164 1095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3225 3986 5327 3225
Size: 761 1341 3225
Fragments arranged by size:
3225 1341 761
BclI T'GATC_A
Cuts at: 2847 2847
Size: 5327
BglI GCCn_nnn'nGGC
Cuts at: 135 171 255 135
Size: 36 84 5207
Fragments arranged by size:
5207 84 36
BglII A'GATC_T
Cuts at: 2842 4879 2842
Size: 2037 3290
BlpI GC'TnA_GC
Cuts at: 2439 2439
Size: 5327
Bme1580I G_kGCm'C
Cuts at: 275 1002 1380 1631 2952 3045 4101 275
Size: 727 378 251 1321 93 1056 1501
Fragments arranged by size:
1501 1321 1056 727 378 251 93
BmrI ACTGGGnnnn_n'
Cuts at: 1322 1492 1789 2539 2620 2955 5099 1322
Size: 170 297 750 81 335 2144 1550
Fragments arranged by size:
2144 1550 750 335 297 170 81
BmtI G_CTAG'C
Cuts at: 1168 1168
Size: 5327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3694 3694
Size: 5327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 1061 1534 330
Size: 731 473 4123
Fragments arranged by size:
4123 731 473
Bpu10I CC'TnA_GC
Cuts at: 605 988 1169 1729 1907 605
Size: 383 181 560 178 4025
Fragments arranged by size:
4025 560 383 181 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2503 3382 3874 4172 2503
Size: 879 492 298 3658
Fragments arranged by size:
3658 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5031 5031
Size: 5327
BsaAI yAC'GTr
Cuts at: 2680 2680
Size: 5327
BsaBI GATnn'nnATC
Cuts at: 6 2841 4878 6
Size: 2835 2037 455
Fragments arranged by size:
2835 2037 455
BsaHI Gr'CG_yC
Cuts at: 341 353 523 1497 2758 3006 3708 5204 341
Size: 12 170 974 1261 248 702 1496 464
Fragments arranged by size:
1496 1261 974 702 464 248 170 12
BsaWI w'CCGG_w
Cuts at: 1321 1955 2516 3037 3715 3989 4136 4547 1321
Size: 634 561 521 678 274 147 411 2101
Fragments arranged by size:
2101 678 634 561 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 327 428 855 1996 327
Size: 101 427 1141 3658
Fragments arranged by size:
3658 1141 427 101
BseYI C'CCAG_C
Cuts at: 2752 3510 4087 4583 2752
Size: 758 577 496 3496
Fragments arranged by size:
3496 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 1508 929
Size: 579 4748
BsiEI CG_ry'CG
Cuts at: 104 563 2869 2915 4123 104
Size: 459 2306 46 1208 1308
Fragments arranged by size:
2306 1308 1208 459 46
BsiHKAI G_wGCw'C
Cuts at: 1456 1511 2049 2341 3119 3309 4101 1456
Size: 55 538 292 778 190 792 2682
Fragments arranged by size:
2682 792 778 538 292 190 55
BsmI GAATG_Cn'
Cuts at: 1282 2202 1282
Size: 920 4407
Bsp1286I G_dGCh'C
Cuts at: 157 275 390 761 989 1002 1319 1380 1456
Size: 118 115 371 228 13 317 61 76
Cuts at: 1456 1511 1526 1631 2049 2341 2952 3045 3119
Size: 55 15 105 418 292 611 93 74
Cuts at: 3119 3309 3371 4101 157
Size: 190 62 730 1383
Fragments arranged by size:
1383 730 611 418 371 317 292 228
190 118 115 105 93 76 74 62
61 55 15 13
BspEI T'CCGG_A
Cuts at: 1321 1955 4547 1321
Size: 634 2592 2101
Fragments arranged by size:
2592 2101 634
BspHI T'CATG_A
Cuts at: 4503 4503
Size: 5327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 398 2377 2591 2893 3274 398
Size: 1979 214 302 381 2451
Fragments arranged by size:
2451 1979 381 302 214
BsrBI CCG'CTC
Cuts at: 50 317 3619 3673 5157 5322 50
Size: 267 3302 54 1484 165 55
Fragments arranged by size:
3302 1484 267 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1088 1868 3239 1088
Size: 780 1371 3176
Fragments arranged by size:
3176 1371 780
BsrFI r'CCGG_y
Cuts at: 180 249 516 3325 3506 3715 180
Size: 69 267 2809 181 209 1792
Fragments arranged by size:
2809 1792 267 209 181 69
BsrGI T'GTAC_A
Cuts at: 1349 1349
Size: 5327
BssHII G'CGCG_C
Cuts at: 301 450 301
Size: 149 5178
BssSI C'ACGA_G
Cuts at: 3598 3956 3598
Size: 358 4969
BstAPI GCAn_nnn'nTGC
Cuts at: 481 2092 2704 481
Size: 1611 612 3104
Fragments arranged by size:
3104 1611 612
BstBI TT'CG_AA
Cuts at: 3688 3688
Size: 5327
BstXI CCAn_nnnn'nTGG
Cuts at: 1248 1944 2097 1248
Size: 696 153 4478
Fragments arranged by size:
4478 696 153
BstYI r'GATC_y
Cuts at: 1 1666 1807 2352 2819 2842 3177 3423 4435
Size: 1665 141 545 467 23 335 246 1012
Cuts at: 4435 4521 4533 4879 1
Size: 86 12 346 449
Fragments arranged by size:
1665 1012 545 467 449 346 335 246
141 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 244 244
Size: 5327
BtgI C'CryG_G
Cuts at: 200 1388 200
Size: 1188 4139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3277 3458 3621 3277
Size: 181 163 4983
Fragments arranged by size:
4983 181 163
BtsI GCAGTG_nn'
Cuts at: 941 1380 1949 2570 941
Size: 439 569 621 3698
Fragments arranged by size:
3698 621 569 439
DraI TTT'AAA
Cuts at: 2326 2326
Size: 5327
DrdI GACnn_nn'nnGTC
Cuts at: 3033 3891 3033
Size: 858 4469
EaeI y'GGCC_r
Cuts at: 127 203 560 1049 1770 1934 2912 3086 3477
Size: 76 357 489 721 164 978 174 391
Cuts at: 3477 3504 4849 127
Size: 27 1345 605
Fragments arranged by size:
1345 978 721 605 489 391 357 174
164 76 27
EagI C'GGCC_G
Cuts at: 560 2912 560
Size: 2352 2975
EarI CTCTTCn'nnn_
Cuts at: 1407 2249 3350 3560 1407
Size: 842 1101 210 3174
Fragments arranged by size:
3174 1101 842 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3535 3845 3991 5137 3535
Size: 310 146 1146 3725
Fragments arranged by size:
3725 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1454 1509 2339 1454
Size: 55 830 4442
Fragments arranged by size:
4442 830 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 806 1061 1534 1949 3152 3584 4331 4599
Size: 476 255 473 415 1203 432 747 268
Cuts at: 4599 330
Size: 1058
Fragments arranged by size:
1203 1058 747 476 473 432 415 268
255
EcoO109I rG'GnC_Cy
Cuts at: 115 221 271 998 2466 115
Size: 106 50 727 1468 2976
Fragments arranged by size:
2976 1468 727 106 50
EcoRI G'AATT_C
Cuts at: 2331 2331
Size: 5327
EcoRV GAT'ATC
Cuts at: 1111 1111
Size: 5327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 3346 2278
Size: 1068 4259
FspI TGC'GCA
Cuts at: 3108 3108
Size: 5327
HaeII r_GCGC'y
Cuts at: 344 437 526 612 960 1500 2761 3009 4031
Size: 93 89 86 348 540 1261 248 1022
Cuts at: 4031 5010 344
Size: 979 661
Fragments arranged by size:
1261 1022 979 661 540 348 248 93
89 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1812 1844 1879 1911 3169 3199 3201 3231 1812
Size: 32 35 32 1258 30 2 30 3908
Fragments arranged by size:
3908 1258 35 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2366 2366
Size: 5327
HindIII A'AGCT_T
Cuts at: 1566 1566
Size: 5327
KasI G'GCGC_C
Cuts at: 340 522 1496 2757 3005 340
Size: 182 974 1261 248 2662
Fragments arranged by size:
2662 1261 974 248 182
KpnI G_GTAC'C
Cuts at: 2347 2347
Size: 5327
MluI A'CGCG_T
Cuts at: 4760 4760
Size: 5327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 273 612 1410 1968 3998 4182 273
Size: 339 798 558 2030 184 1418
Fragments arranged by size:
2030 1418 798 558 339 184
MscI TGG'CCA
Cuts at: 1051 1772 1936 3088 1051
Size: 721 164 1152 3290
Fragments arranged by size:
3290 1152 721 164
MslI CAynn'nnrTG
Cuts at: 1021 1387 3316 3443 1021
Size: 366 1929 127 2905
Fragments arranged by size:
2905 1929 366 127
MspA1I CmG'CkG
Cuts at: 132 202 308 404 481 559 625 1906 2028
Size: 70 106 96 77 78 66 1281 122
Cuts at: 2028 2438 2752 3112 4125 4370 132
Size: 410 314 360 1013 245 1089
Fragments arranged by size:
1281 1089 1013 410 360 314 245 122
106 96 78 77 70 66
NaeI GCC'GGC
Cuts at: 251 518 3508 251
Size: 267 2990 2070
Fragments arranged by size:
2990 2070 267
NarI GG'CG_CC
Cuts at: 341 523 1497 2758 3006 341
Size: 182 974 1261 248 2662
Fragments arranged by size:
2662 1261 974 248 182
NgoMIV G'CCGG_C
Cuts at: 249 516 3506 249
Size: 267 2990 2070
Fragments arranged by size:
2990 2070 267
NheI G'CTAG_C
Cuts at: 1164 1164
Size: 5327
NspI r_CATG'y
Cuts at: 2380 2880 3787 2380
Size: 500 907 3920
Fragments arranged by size:
3920 907 500
PciI A'CATG_T
Cuts at: 3783 3783
Size: 5327
PflMI CCAn_nnn'nTGG
Cuts at: 1923 1923
Size: 5327
PfoI T'CCnGG_A
Cuts at: 569 1511 569
Size: 942 4385
PmeI GTTT'AAAC
Cuts at: 2326 2326
Size: 5327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 5295
PpuMI rG'GwC_Cy
Cuts at: 221 221
Size: 5327
PshAI GACnn'nnGTC
Cuts at: 1129 1781 1129
Size: 652 4675
PspOMI G'GGCC_C
Cuts at: 271 998 271
Size: 727 4600
PspXI vC'TCGA_Gb
Cuts at: 142 142
Size: 5327
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1181 1213 1181
Size: 32 5295
PstI C_TGCA'G
Cuts at: 409 2374 3059 409
Size: 1965 685 2677
Fragments arranged by size:
2677 1965 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5327
PvuII CAG'CTG
Cuts at: 404 625 2752 3112 404
Size: 221 2127 360 2619
Fragments arranged by size:
2619 2127 360 221
RsrII CG'GwC_CG
Cuts at: 3522 3522
Size: 5327
SacI G_AGCT'C
Cuts at: 1456 1511 2341 1456
Size: 55 830 4442
Fragments arranged by size:
4442 830 55
SacII CC_GC'GG
Cuts at: 203 203
Size: 5327
SalI G'TCGA_C
Cuts at: 2364 2364
Size: 5327
SanDI GG'GwC_CC
Cuts at: 221 221
Size: 5327
SapI GCTCTTCn'nnn_
Cuts at: 2249 3350 3560 2249
Size: 1101 210 4016
Fragments arranged by size:
4016 1101 210
SbfI CC_TGCA'GG
Cuts at: 2374 2374
Size: 5327
ScaI AGT'ACT
Cuts at: 4844 4844
Size: 5327
SfcI C'TryA_G
Cuts at: 33 405 726 2370 3055 4048 4239 33
Size: 372 321 1644 685 993 191 1121
Fragments arranged by size:
1644 1121 993 685 372 321 191
SfoI GGC'GCC
Cuts at: 342 524 1498 2759 3007 342
Size: 182 974 1261 248 2662
Fragments arranged by size:
2662 1261 974 248 182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5327
SmaI CCC'GGG
Cuts at: 219 464 531 1291 2349 219
Size: 245 67 760 1058 3197
Fragments arranged by size:
3197 1058 760 245 67
SmlI C'TyrA_G
Cuts at: 142 2482 3397 3889 4151 142
Size: 2340 915 492 262 1318
Fragments arranged by size:
2340 1318 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5327
SphI G_CATG'C
Cuts at: 2380 2880 2380
Size: 500 4827
SrfI GCCC'GGGC
Cuts at: 1291 1291
Size: 5327
StuI AGG'CCT
Cuts at: 502 502
Size: 5327
StyI C'CwwG_G
Cuts at: 753 1968 2223 2461 2815 753
Size: 1215 255 238 354 3265
Fragments arranged by size:
3265 1215 354 255 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1381 1474 2910 3043 3502 3711 1381
Size: 93 1436 133 459 209 2997
Fragments arranged by size:
2997 1436 459 209 133 93
TatI w'GTAC_w
Cuts at: 1349 4842 1349
Size: 3493 1834
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 4844 4876 2144
Size: 32 2668 32 2595
Fragments arranged by size:
2668 2595 32 32
Tth111I GACn'n_nGTC
Cuts at: 3124 3124
Size: 5327
XbaI T'CTAG_A
Cuts at: 2133 2358 2133
Size: 225 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 717 2822 717
Size: 2105 3222
XhoI C'TCGA_G
Cuts at: 142 142
Size: 5327
XmaI C'CCGG_G
Cuts at: 217 462 529 1289 2347 217
Size: 245 67 760 1058 3197
Fragments arranged by size:
3197 1058 760 245 67
XmnI GAAnn'nnTTC
Cuts at: 1567 1567
Size: 5327
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI BaeI BbsI
BfrBI BmgBI BsaXI BsiWI BsmBI BstEII BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PmlI PsiI
SexAI SfiI SgrAI SnaBI SspI SwaI ZraI