Restriction map for
pF1KE2348
(Circular) (Six-base) MAPSORT of: pF1KE2348.seq Check: 7521 from: 1 to: 7424
pF1KE2348 7424 bp
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1829 3639 1829
Size: 1810 5614
AccI GT'mk_AC
Cuts at: 727 2651 4462 727
Size: 1924 1811 3689
Fragments arranged by size:
3689 1924 1811
Acc65I G'GTAC_C
Cuts at: 3710 4440 3710
Size: 730 6694
AclI AA'CG_TT
Cuts at: 3095 7264 3095
Size: 4169 3255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 672 2715 3244 4013 4091 5249 5681 6428
Size: 281 2043 529 769 78 1158 432 747
Cuts at: 6428 6696 391
Size: 268 1119
Fragments arranged by size:
2043 1158 1119 769 747 529 432 281
268 78
AflII C'TTAA_G
Cuts at: 895 895
Size: 7424
AflIII A'CryG_T
Cuts at: 503 1167 3382 4776 5880 6857 503
Size: 664 2215 1394 1104 977 1070
Fragments arranged by size:
2215 1394 1104 1070 977 664
AgeI A'CCGG_T
Cuts at: 844 5812 844
Size: 4968 2456
AhdI GACnn_n'nnGTC
Cuts at: 507 507
Size: 7424
AleI CACnn'nnGTG
Cuts at: 1414 2782 3583 1414
Size: 1368 801 5255
Fragments arranged by size:
5255 1368 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2864 2864
Size: 7424
AlwNI CAG_nnn'CTG
Cuts at: 598 1495 2090 2346 2738 3345 4729 6296 598
Size: 897 595 256 392 607 1384 1567 1726
Fragments arranged by size:
1726 1567 1384 897 607 595 392 256
ApaI G_GGCC'C
Cuts at: 2674 3167 2674
Size: 493 6931
ApaLI G'TGCA_C
Cuts at: 6194 6194
Size: 7424
ApoI r'AATT_y
Cuts at: 1204 2421 3966 4428 6777 7033 1204
Size: 1217 1545 462 2349 256 1595
Fragments arranged by size:
2349 1595 1545 1217 462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7424
AvaI C'yCGr_G
Cuts at: 269 2195 2789 4444 269
Size: 1926 594 1655 3249
Fragments arranged by size:
3249 1926 1655 594
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1605 1638 1605
Size: 33 7391
BamHI G'GATC_C
Cuts at: 2006 2969 4449 2006
Size: 963 1480 4981
Fragments arranged by size:
4981 1480 963
BanI G'GyrC_C
Cuts at: 2401 3710 4440 4854 5102 5137 2401
Size: 1309 730 414 248 35 4688
Fragments arranged by size:
4688 1309 730 414 248 35
BanII G_rGCy'C
Cuts at: 491 935 2674 3167 4137 4438 5468 491
Size: 444 1739 493 970 301 1030 2447
Fragments arranged by size:
2447 1739 1030 970 493 444 301
BbeI G_GCGC'C
Cuts at: 4858 5106 4858
Size: 248 7176
BbsI GAAGACnn'nnnn_
Cuts at: 506 521 555 1416 3129 4001 506
Size: 15 34 861 1713 872 3929
Fragments arranged by size:
3929 1713 872 861 34 15
BciVI GTATCCnnnnn_n'
Cuts at: 1633 3756 5322 6083 7424 1633
Size: 2123 1566 761 1341 1633
Fragments arranged by size:
2123 1633 1566 1341 761
BclI T'GATC_A
Cuts at: 668 4944 668
Size: 4276 3148
BfrBI ATG'CAT
Cuts at: 303 303
Size: 7424
BglI GCCn_nnn'nGGC
Cuts at: 469 1492 1597 1999 469
Size: 1023 105 402 5894
Fragments arranged by size:
5894 1023 402 105
BglII A'GATC_T
Cuts at: 4939 6976 4939
Size: 2037 5387
BlpI GC'TnA_GC
Cuts at: 4046 4536 4046
Size: 490 6934
Bme1580I G_kGCm'C
Cuts at: 2633 2674 2926 3167 5049 5142 6198 2633
Size: 41 252 241 1882 93 1056 3859
Fragments arranged by size:
3859 1882 1056 252 241 93 41
BmgBI CAC'GTC
Cuts at: 504 537 3057 3272 504
Size: 33 2520 215 4656
Fragments arranged by size:
4656 2520 215 33
BmrI ACTGGGnnnn_n'
Cuts at: 140 400 1772 4636 4717 5052 7196 140
Size: 260 1372 2864 81 335 2144 368
Fragments arranged by size:
2864 2144 1372 368 335 260 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 300 3739 4156 5791 300
Size: 3439 417 1635 1933
Fragments arranged by size:
3439 1933 1635 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1602 1876 3186 3869 4036 1022
Size: 580 274 1310 683 167 4410
Fragments arranged by size:
4410 1310 683 580 274 167
Bpu10I CC'TnA_GC
Cuts at: 2161 2161
Size: 7424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 2287 3914 4600 5479 5971 6269 381
Size: 1906 1627 686 879 492 298 1536
Fragments arranged by size:
1906 1627 1536 879 686 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 194 515 2794 3502 4210 7128 194
Size: 321 2279 708 708 2918 490
Fragments arranged by size:
2918 2279 708 708 490 321
BsaAI yAC'GTr
Cuts at: 3383 4777 3383
Size: 1394 6030
BsaBI GATnn'nnATC
Cuts at: 6 3085 4938 6975 6
Size: 3079 1853 2037 455
Fragments arranged by size:
3079 2037 1853 455
BsaHI Gr'CG_yC
Cuts at: 4855 5103 5805 7301 4855
Size: 248 702 1496 4978
Fragments arranged by size:
4978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 380 844 2291 4613 5134 5812 6086 6233 6644
Size: 464 1447 2322 521 678 274 147 411
Cuts at: 6644 380
Size: 1160
Fragments arranged by size:
2322 1447 1160 678 521 464 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 931 961 995 1025 2851 2881 3184 3214 931
Size: 30 34 30 1826 30 303 30 5141
Fragments arranged by size:
5141 1826 303 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 772 1279 1282 1599 1744 2165 2918 343
Size: 429 507 3 317 145 421 753 4849
Fragments arranged by size:
4849 753 507 429 421 317 145 3
BseYI C'CCAG_C
Cuts at: 418 774 1098 1994 2119 2463 2665 3483 3813
Size: 356 324 896 125 344 202 818 330
Cuts at: 3813 4849 5607 6184 6680 418
Size: 1036 758 577 496 1162
Fragments arranged by size:
1162 1036 896 818 758 577 496 356
344 330 324 202 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2620 3175 3313 4255 1825
Size: 795 555 138 942 4994
Fragments arranged by size:
4994 942 795 555 138
BsiEI CG_ry'CG
Cuts at: 104 235 4966 5012 6220 104
Size: 131 4731 46 1208 1308
Fragments arranged by size:
4731 1308 1208 131 46
BsiHKAI G_wGCw'C
Cuts at: 369 491 935 4438 5216 5406 6198 369
Size: 122 444 3503 778 190 792 1595
Fragments arranged by size:
3503 1595 792 778 444 190 122
BsmI GAATG_Cn'
Cuts at: 247 303 2680 247
Size: 56 2377 4991
Fragments arranged by size:
4991 2377 56
Bsp1286I G_dGCh'C
Cuts at: 369 491 935 2633 2674 2926 3167 4137 4438
Size: 122 444 1698 41 252 241 970 301
Cuts at: 4438 5049 5142 5216 5406 5468 6198 369
Size: 611 93 74 190 62 730 1595
Fragments arranged by size:
1698 1595 970 730 611 444 301 252
241 190 122 93 74 62 41
BspEI T'CCGG_A
Cuts at: 380 2291 6644 380
Size: 1911 4353 1160
Fragments arranged by size:
4353 1911 1160
BspHI T'CATG_A
Cuts at: 2372 6600 2372
Size: 4228 3196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 758 1829 3639 4474 4688 4990 5371 758
Size: 1071 1810 835 214 302 381 2811
Fragments arranged by size:
2811 1810 1071 835 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1773 2165 5716 5770 7254 7419 50
Size: 1723 392 3551 54 1484 165 55
Fragments arranged by size:
3551 1723 1484 392 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1109 1961 4256 5336 1109
Size: 852 2295 1080 3197
Fragments arranged by size:
3197 2295 1080 852
BsrFI r'CCGG_y
Cuts at: 844 3936 5422 5603 5812 844
Size: 3092 1486 181 209 2456
Fragments arranged by size:
3092 2456 1486 209 181
BssSI C'ACGA_G
Cuts at: 523 5695 6053 523
Size: 5172 358 1894
Fragments arranged by size:
5172 1894 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2598 4801 2598
Size: 2203 5221
BstBI TT'CG_AA
Cuts at: 2976 5785 2976
Size: 2809 4615
BstEII G'GTnAC_C
Cuts at: 1353 3684 1353
Size: 2331 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 1335 2665 3042 1335
Size: 1330 377 5717
Fragments arranged by size:
5717 1330 377
BstYI r'GATC_y
Cuts at: 1 623 890 1859 2006 2969 4449 4916 4939
Size: 622 267 969 147 963 1480 467 23
Cuts at: 4939 5274 5520 6532 6618 6630 6976 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1480 1012 969 963 622 467 449 346
335 267 246 147 86 23 12
BstZ17I GTA'TAC
Cuts at: 728 728
Size: 7424
Bsu36I CC'TnA_GG
Cuts at: 2095 2095
Size: 7424
BtgI C'CryG_G
Cuts at: 875 1157 2870 3142 3443 4184 875
Size: 282 1713 272 301 741 4115
Fragments arranged by size:
4115 1713 741 301 282 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3403 3411 4249 5374 5555 5718 3403
Size: 8 838 1125 181 163 5109
Fragments arranged by size:
5109 1125 838 181 163 8
BtsI GCAGTG_nn'
Cuts at: 1837 1899 3164 4667 1837
Size: 62 1265 1503 4594
Fragments arranged by size:
4594 1503 1265 62
ClaI AT'CG_AT
Cuts at: 1850 1850
Size: 7424
DraI TTT'AAA
Cuts at: 2426 4423 2426
Size: 1997 5427
DraIII CAC_nnn'GTG
Cuts at: 408 2741 408
Size: 2333 5091
DrdI GACnn_nn'nnGTC
Cuts at: 5130 5988 5130
Size: 858 6566
EaeI y'GGCC_r
Cuts at: 1151 2108 5009 5183 5574 5601 6946 1151
Size: 957 2901 174 391 27 1345 1629
Fragments arranged by size:
2901 1629 1345 957 391 174 27
EagI C'GGCC_G
Cuts at: 5009 5009
Size: 7424
EarI CTCTTCn'nnn_
Cuts at: 566 1523 1781 2603 3832 5447 5657 566
Size: 957 258 822 1229 1615 210 2333
Fragments arranged by size:
2333 1615 1229 957 822 258 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3804 4132 5632 5942 6088 7234 3804
Size: 328 1500 310 146 1146 3994
Fragments arranged by size:
3994 1500 1146 328 310 146
EcoICRI GAG'CTC
Cuts at: 489 933 4436 489
Size: 444 3503 3477
Fragments arranged by size:
3503 3477 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 672 1022 1602 1876 2715 3186 3244 3869
Size: 281 350 580 274 839 471 58 625
Cuts at: 3869 4013 4036 4091 5249 5681 6428 6696 391
Size: 144 23 55 1158 432 747 268 1119
Fragments arranged by size:
1158 1119 839 747 625 580 471 432
350 281 274 268 144 58 55 23
EcoNI CCTnn'n_nnAGG
Cuts at: 325 2546 325
Size: 2221 5203
EcoO109I rG'GnC_Cy
Cuts at: 279 1006 2539 2670 2671 3163 3164 3207 3285
Size: 727 1533 131 1 492 1 43 78
Cuts at: 3285 4563 279
Size: 1278 3140
Fragments arranged by size:
3140 1533 1278 727 492 131 78 43
1 1
EcoRI G'AATT_C
Cuts at: 4428 4428
Size: 7424
EcoRV GAT'ATC
Cuts at: 117 117
Size: 7424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 5443 2611
Size: 2832 4592
FspI TGC'GCA
Cuts at: 5205 5205
Size: 7424
HaeII r_GCGC'y
Cuts at: 2178 4858 5106 6128 7107 2178
Size: 2680 248 1022 979 2495
Fragments arranged by size:
2680 2495 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 2018 2050 2894 2926 3555 3582 3587
Size: 32 1493 32 844 32 629 27 5
Cuts at: 3587 3614 3842 3863 3874 3895 4081 4113 4324
Size: 27 228 21 11 21 186 32 211
Cuts at: 4324 4356 5266 5296 5298 5328 493
Size: 32 910 30 2 30 2589
Fragments arranged by size:
2589 1493 910 844 629 228 211 186
32 32 32 32 32 30 30 27
27 21 21 11 5 2
HincII GTy'rAC
Cuts at: 1500 2352 3054 3524 4463 1500
Size: 852 702 470 939 4461
Fragments arranged by size:
4461 939 852 702 470
HpaI GTT'AAC
Cuts at: 3524 3524
Size: 7424
KasI G'GCGC_C
Cuts at: 4854 5102 4854
Size: 248 7176
KpnI G_GTAC'C
Cuts at: 3714 4444 3714
Size: 730 6694
MluI A'CGCG_T
Cuts at: 6857 6857
Size: 7424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 974 1255 1860 4379 6095 6279 218
Size: 756 281 605 2519 1716 184 1363
Fragments arranged by size:
2519 1716 1363 756 605 281 184
MscI TGG'CCA
Cuts at: 2110 5185 2110
Size: 3075 4349
MslI CAynn'nnrTG
Cuts at: 1312 1333 1414 2782 3583 5413 5540 1312
Size: 21 81 1368 801 1830 127 3196
Fragments arranged by size:
3196 1830 1368 801 127 81 21
MspA1I CmG'CkG
Cuts at: 678 1078 1704 2087 2604 4180 4535 4849 5209
Size: 400 626 383 517 1576 355 314 360
Cuts at: 5209 6222 6467 678
Size: 1013 245 1635
Fragments arranged by size:
1635 1576 1013 626 517 400 383 360
355 314 245
NaeI GCC'GGC
Cuts at: 5605 5605
Size: 7424
NarI GG'CG_CC
Cuts at: 4855 5103 4855
Size: 248 7176
NcoI C'CATG_G
Cuts at: 875 2870 4184 875
Size: 1995 1314 4115
Fragments arranged by size:
4115 1995 1314
NdeI CA'TA_TG
Cuts at: 1370 2837 1370
Size: 1467 5957
NgoMIV G'CCGG_C
Cuts at: 5603 5603
Size: 7424
NsiI A_TGCA'T
Cuts at: 305 305
Size: 7424
NspI r_CATG'y
Cuts at: 4477 4977 5884 4477
Size: 500 907 6017
Fragments arranged by size:
6017 907 500
PasI CC'CwG_GG
Cuts at: 924 1377 4311 924
Size: 453 2934 4037
Fragments arranged by size:
4037 2934 453
PciI A'CATG_T
Cuts at: 5880 5880
Size: 7424
PfoI T'CCnGG_A
Cuts at: 320 1757 2228 320
Size: 1437 471 5516
Fragments arranged by size:
5516 1437 471
PmeI GTTT'AAAC
Cuts at: 4423 4423
Size: 7424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2820 4100 4132 6563 6595 2788
Size: 32 1280 32 2431 32 3617
Fragments arranged by size:
3617 2431 1280 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1006 2539 3207 1006
Size: 1533 668 5223
Fragments arranged by size:
5223 1533 668
PshAI GACnn'nnGTC
Cuts at: 2537 2537
Size: 7424
PsiI TTA'TAA
Cuts at: 1037 1037
Size: 7424
PspOMI G'GGCC_C
Cuts at: 2670 3163 2670
Size: 493 6931
PspXI vC'TCGA_Gb
Cuts at: 2789 2789
Size: 7424
PstI C_TGCA'G
Cuts at: 547 755 1709 4395 4471 5156 547
Size: 208 954 2686 76 685 2815
Fragments arranged by size:
2815 2686 954 685 208 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7424
PvuII CAG'CTG
Cuts at: 678 1078 1704 2087 2604 4180 4849 5209 678
Size: 400 626 383 517 1576 669 360 2893
Fragments arranged by size:
2893 1576 669 626 517 400 383 360
RsrII CG'GwC_CG
Cuts at: 5619 5619
Size: 7424
SacI G_AGCT'C
Cuts at: 491 935 4438 491
Size: 444 3503 3477
Fragments arranged by size:
3503 3477 444
SalI G'TCGA_C
Cuts at: 4461 4461
Size: 7424
SapI GCTCTTCn'nnn_
Cuts at: 566 5447 5657 566
Size: 4881 210 2333
Fragments arranged by size:
4881 2333 210
SbfI CC_TGCA'GG
Cuts at: 4471 4471
Size: 7424
ScaI AGT'ACT
Cuts at: 2845 6941 2845
Size: 4096 3328
SexAI A'CCwGG_T
Cuts at: 2345 2737 2345
Size: 392 7032
SfcI C'TryA_G
Cuts at: 33 543 751 1705 4391 4467 5152 6145 6336
Size: 510 208 954 2686 76 685 993 191
Cuts at: 6336 33
Size: 1121
Fragments arranged by size:
2686 1121 993 954 685 510 208 191
76
SfiI GGCCn_nnn'nGGCC
Cuts at: 469 1999 469
Size: 1530 5894
SfoI GGC'GCC
Cuts at: 4856 5104 4856
Size: 248 7176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7424
SmaI CCC'GGG
Cuts at: 2197 4446 2197
Size: 2249 5175
SmlI C'TyrA_G
Cuts at: 360 895 2302 2789 3929 4579 5494 5986 6248
Size: 535 1407 487 1140 650 915 492 262
Cuts at: 6248 360
Size: 1536
Fragments arranged by size:
1536 1407 1140 915 650 535 492 487
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7424
SphI G_CATG'C
Cuts at: 4477 4977 4477
Size: 500 6924
SrfI GCCC'GGGC
Cuts at: 2197 2197
Size: 7424
StuI AGG'CCT
Cuts at: 2002 2100 2002
Size: 98 7326
StyI C'CwwG_G
Cuts at: 254 338 875 1595 2675 2870 3620 4184 4558
Size: 84 537 720 1080 195 750 564 374
Cuts at: 4558 4912 254
Size: 354 2766
Fragments arranged by size:
2766 1080 750 720 564 537 374 354
195 84
SwaI ATTT'AAAT
Cuts at: 2426 2426
Size: 7424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 419 916 1306 2419 4332 5007 5140 5599 5808
Size: 497 390 1113 1913 675 133 459 209
Cuts at: 5808 419
Size: 2035
Fragments arranged by size:
2035 1913 1113 675 497 459 390 209
133
TatI w'GTAC_w
Cuts at: 755 2843 3986 6939 755
Size: 2088 1143 2953 1240
Fragments arranged by size:
2953 2088 1240 1143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 3184 3216 3256 3288 4307 4339 6941
Size: 32 2662 32 40 32 1019 32 2602
Cuts at: 6941 6973 490
Size: 32 941
Fragments arranged by size:
2662 2602 1019 941 40 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3615 5221 3615
Size: 1606 5818
XbaI T'CTAG_A
Cuts at: 1950 4455 1950
Size: 2505 4919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 873 4182 4919 873
Size: 3309 737 3378
Fragments arranged by size:
3378 3309 737
XhoI C'TCGA_G
Cuts at: 2789 2789
Size: 7424
XmaI C'CCGG_G
Cuts at: 2195 4444 2195
Size: 2249 5175
XmnI GAAnn'nnTTC
Cuts at: 1532 1532
Size: 7424
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI AscI AvrII BbvCI BcgI BmtI
BsiWI BsmBI BsrGI BssHII CspCI FseI FspAI HindIII
MfeI NheI NotI NruI PacI PflMI PmlI PsrI
SacII SanDI SgrAI SnaBI SspI ZraI