Restriction map for
pF1KE2354
(Circular) (Six-base) MAPSORT of: pF1KE2354.seq Check: 8479 from: 1 to: 12785
pF1KE2354 12785 bp
With 182 enzymes: SgfI *
February 15, 2013 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4668 5049 5226 5400 4668
Size: 381 177 174 12053
Fragments arranged by size:
12053 381 177 174
AatII G_ACGT'C
Cuts at: 602 602
Size: 12785
AccI GT'mk_AC
Cuts at: 2015 4635 8196 9823 2015
Size: 2620 3561 1627 4977
Fragments arranged by size:
4977 3561 2620 1627
Acc65I G'GTAC_C
Cuts at: 8466 9801 8466
Size: 1335 11450
AclI AA'CG_TT
Cuts at: 12625 12625
Size: 12785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1393 1837 2231 2711 3305 4667 4856 5033
Size: 504 444 394 480 594 1362 189 177
Cuts at: 5033 5210 5546 5954 6412 6524 6638 6897 6959
Size: 177 336 408 458 112 114 259 62
Cuts at: 6959 7568 7856 7997 8477 8507 9378 9655 10610
Size: 609 288 141 480 30 871 277 955
Cuts at: 10610 11042 11789 12057 889
Size: 432 747 268 1617
Fragments arranged by size:
1617 1362 955 871 747 609 594 504
480 480 458 444 432 408 394 336
288 277 268 259 189 177 177 141
114 112 62 30
AflII C'TTAA_G
Cuts at: 739 1188 739
Size: 449 12336
AflIII A'CryG_T
Cuts at: 1175 4721 9258 10137 11241 12218 1175
Size: 3546 4537 879 1104 977 1742
Fragments arranged by size:
4537 3546 1742 1104 977 879
AgeI A'CCGG_T
Cuts at: 11173 11173
Size: 12785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2867 2867
Size: 12785
AlwNI CAG_nnn'CTG
Cuts at: 1741 2662 4849 6028 7666 7729 10090 11657 1741
Size: 921 2187 1179 1638 63 2361 1567 2869
Fragments arranged by size:
2869 2361 2187 1638 1567 1179 921 63
ApaI G_GGCC'C
Cuts at: 2809 6767 2809
Size: 3958 8827
ApaLI G'TGCA_C
Cuts at: 11555 11555
Size: 12785
ApoI r'AATT_y
Cuts at: 717 1440 2529 2963 3106 3202 3213 3431 3471
Size: 723 1089 434 143 96 11 218 40
Cuts at: 3471 3685 3794 4134 4141 4480 5686 5709 5749
Size: 214 109 340 7 339 1206 23 40
Cuts at: 5749 5803 6290 6481 6508 6520 7409 8712 8900
Size: 54 487 191 27 12 889 1303 188
Cuts at: 8900 9234 9435 9532 9546 9789 12138 12394 717
Size: 334 201 97 14 243 2349 256 1108
Fragments arranged by size:
2349 1303 1206 1108 1089 889 723 487
434 340 339 334 256 243 218 214
201 191 188 143 109 97 96 54
40 40 27 23 14 12 11 7
AseI AT'TA_AT
Cuts at: 20 266 1105 20
Size: 246 839 11700
Fragments arranged by size:
11700 839 246
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12785
AvaI C'yCGr_G
Cuts at: 9805 9805
Size: 12785
BamHI G'GATC_C
Cuts at: 282 9017 9810 282
Size: 8735 793 3257
Fragments arranged by size:
8735 3257 793
BanI G'GyrC_C
Cuts at: 6721 8466 9801 10215 10463 10498 6721
Size: 1745 1335 414 248 35 9008
Fragments arranged by size:
9008 1745 1335 414 248 35
BanII G_rGCy'C
Cuts at: 160 1041 1800 2700 2809 4262 4594 5581 6767
Size: 881 759 900 109 1453 332 987 1186
Cuts at: 6767 8651 9799 10829 160
Size: 1884 1148 1030 2116
Fragments arranged by size:
2116 1884 1453 1186 1148 1030 987 900
881 759 332 109
BbeI G_GCGC'C
Cuts at: 10219 10467 10219
Size: 248 12537
BbsI GAAGACnn'nnnn_
Cuts at: 301 1399 2057 2189 2375 4724 7862 8348 8366
Size: 1098 658 132 186 2349 3138 486 18
Cuts at: 8366 301
Size: 4720
Fragments arranged by size:
4720 3138 2349 1098 658 486 186 132
18
BbvCI CC'TCA_GC
Cuts at: 6248 6248
Size: 12785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1363 1397 1363
Size: 34 12751
BciVI GTATCCnnnnn_n'
Cuts at: 1290 7083 9690 10683 11444 12785 1290
Size: 5793 2607 993 761 1341 1290
Fragments arranged by size:
5793 2607 1341 1290 993 761
BclI T'GATC_A
Cuts at: 1887 6666 10305 1887
Size: 4779 3639 4367
Fragments arranged by size:
4779 4367 3639
BfrBI ATG'CAT
Cuts at: 981 981
Size: 12785
BglII A'GATC_T
Cuts at: 390 1554 3082 3892 4494 4525 5952 10300 12337
Size: 1164 1528 810 602 31 1427 4348 2037
Cuts at: 12337 390
Size: 838
Fragments arranged by size:
4348 2037 1528 1427 1164 838 810 602
31
BlpI GC'TnA_GC
Cuts at: 8033 9897 8033
Size: 1864 10921
Bme1580I G_kGCm'C
Cuts at: 1175 2321 2809 3591 6767 10410 10503 11559 1175
Size: 1146 488 782 3176 3643 93 1056 2401
Fragments arranged by size:
3643 3176 2401 1146 1056 782 488 93
BmgBI CAC'GTC
Cuts at: 129 129
Size: 12785
BmrI ACTGGGnnnn_n'
Cuts at: 2757 2803 4371 5584 6770 7739 8763 9997 10078
Size: 46 1568 1213 1186 969 1024 1234 81
Cuts at: 10078 10413 12557 2757
Size: 335 2144 2985
Fragments arranged by size:
2985 2144 1568 1234 1213 1186 1024 969
335 81 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 4608 11152 2407
Size: 2201 6544 4040
Fragments arranged by size:
6544 4040 2201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 2344 6050 7253 8702 8753 8903 9232 9431
Size: 1572 3706 1203 1449 51 150 329 199
Cuts at: 9431 772
Size: 4126
Fragments arranged by size:
4126 3706 1572 1449 1203 329 199 150
51
Bpu10I CC'TnA_GC
Cuts at: 5879 6248 5879
Size: 369 12416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1497 6992 9961 10840 11332 11630 1497
Size: 5495 2969 879 492 298 2652
Fragments arranged by size:
5495 2969 2652 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3316 9026 9283 12489 3316
Size: 5710 257 3206 3612
Fragments arranged by size:
5710 3612 3206 257
BsaAI yAC'GTr
Cuts at: 10138 10138
Size: 12785
BsaBI GATnn'nnATC
Cuts at: 6 2288 2669 10299 12336 6
Size: 2282 381 7630 2037 455
Fragments arranged by size:
7630 2282 2037 455 381
BsaHI Gr'CG_yC
Cuts at: 599 4051 10216 10464 11166 12662 599
Size: 3452 6165 248 702 1496 722
Fragments arranged by size:
6165 3452 1496 722 702 248
BsaWI w'CCGG_w
Cuts at: 4812 6085 9014 9974 10495 11173 11447 11594 12005
Size: 1273 2929 960 521 678 274 147 411
Cuts at: 12005 4812
Size: 5592
Fragments arranged by size:
5592 2929 1273 960 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1615 1645 2433 2463 3042 3072 4918 4948 5095
Size: 30 788 30 579 30 1846 30 147
Cuts at: 5095 5125 8726 8756 1615
Size: 30 3601 30 5644
Fragments arranged by size:
5644 3601 1846 788 579 147 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 7628 8134 9494 7628
Size: 506 1360 10919
Fragments arranged by size:
10919 1360 506
BseYI C'CCAG_C
Cuts at: 10210 10968 11545 12041 10210
Size: 758 577 496 10954
Fragments arranged by size:
10954 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 883 172
Size: 711 12074
BsiEI CG_ry'CG
Cuts at: 104 6719 10327 10373 11581 104
Size: 6615 3608 46 1208 1308
Fragments arranged by size:
6615 3608 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 903 1041 1395 4262 4594 9402 9799 10577 10767
Size: 138 354 2867 332 4808 397 778 190
Cuts at: 10767 11559 903
Size: 792 2129
Fragments arranged by size:
4808 2867 2129 792 778 397 354 332
190 138
BsmI GAATG_Cn'
Cuts at: 1852 2230 4206 6329 6535 7708 1852
Size: 378 1976 2123 206 1173 6929
Fragments arranged by size:
6929 2123 1976 1173 378 206
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 3804 2453
Size: 1351 11434
Bsp1286I G_dGCh'C
Cuts at: 160 903 1041 1175 1395 1800 2321 2700 2809
Size: 743 138 134 220 405 521 379 109
Cuts at: 2809 3591 4262 4594 5581 6767 8651 9402 9799
Size: 782 671 332 987 1186 1884 751 397
Cuts at: 9799 10410 10503 10577 10767 10829 11559 160
Size: 611 93 74 190 62 730 1386
Fragments arranged by size:
1884 1386 1186 987 782 751 743 730
671 611 521 405 397 379 332 220
190 138 134 109 93 74 62
BspEI T'CCGG_A
Cuts at: 4812 12005 4812
Size: 7193 5592
BspHI T'CATG_A
Cuts at: 3567 4092 11961 3567
Size: 525 7869 4391
Fragments arranged by size:
7869 4391 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1830 4647 4668 5049 5226 5400 9835 10049 10351
Size: 2817 21 381 177 174 4435 214 302
Cuts at: 10351 10732 1830
Size: 381 3883
Fragments arranged by size:
4435 3883 2817 381 381 302 214 177
174 21
BsrBI CCG'CTC
Cuts at: 50 135 4898 5075 5252 11077 11131 12615 12780
Size: 85 4763 177 177 5825 54 1484 165
Cuts at: 12780 50
Size: 55
Fragments arranged by size:
5825 4763 1484 177 177 165 85 55
54
BsrDI GCAATG_nn'
Cuts at: 1444 2935 3765 4412 10697 1444
Size: 1491 830 647 6285 3532
Fragments arranged by size:
6285 3532 1491 830 647
BsrFI r'CCGG_y
Cuts at: 6718 8776 10783 10964 11173 6718
Size: 2058 2007 181 209 8330
Fragments arranged by size:
8330 2058 2007 209 181
BsrGI T'GTAC_A
Cuts at: 729 1678 1833 5481 729
Size: 949 155 3648 8033
Fragments arranged by size:
8033 3648 949 155
BssSI C'ACGA_G
Cuts at: 11056 11414 11056
Size: 358 12427
BstAPI GCAn_nnn'nTGC
Cuts at: 1179 2868 3073 4760 4949 5126 5303 5956 10162
Size: 1689 205 1687 189 177 177 653 4206
Cuts at: 10162 1179
Size: 3802
Fragments arranged by size:
4206 3802 1689 1687 653 205 189 177
177
BstBI TT'CG_AA
Cuts at: 8138 11146 8138
Size: 3008 9777
BstXI CCAn_nnnn'nTGG
Cuts at: 2513 5902 9300 2513
Size: 3389 3398 5998
Fragments arranged by size:
5998 3398 3389
BstYI r'GATC_y
Cuts at: 1 282 390 1554 3082 3892 4450 4494 4525
Size: 281 108 1164 1528 810 558 44 31
Cuts at: 4525 5952 7807 9017 9810 10277 10300 10635 10881
Size: 1427 1855 1210 793 467 23 335 246
Cuts at: 10881 11893 11979 11991 12337 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1855 1528 1427 1210 1164 1012 810 793
558 467 449 346 335 281 246 108
86 44 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 1292 3971 1292
Size: 2679 10106
BtgI C'CryG_G
Cuts at: 3510 3591 4046 5907 9584 3510
Size: 81 455 1861 3677 6711
Fragments arranged by size:
6711 3677 1861 455 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 126 1331 6146 10735 10916 11079 126
Size: 1205 4815 4589 181 163 1832
Fragments arranged by size:
4815 4589 1832 1205 181 163
BtsI GCAGTG_nn'
Cuts at: 666 4569 4950 5127 5304 7634 9176 10028 666
Size: 3903 381 177 177 2330 1542 852 3423
Fragments arranged by size:
3903 3423 2330 1542 852 381 177 177
ClaI AT'CG_AT
Cuts at: 4326 4326
Size: 12785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 7610 7645 7610
Size: 35 12750
DraI TTT'AAA
Cuts at: 1214 3201 3990 4022 4479 5631 5802 6486 6921
Size: 1987 789 32 457 1152 171 684 435
Cuts at: 6921 7377 7941 8910 9045 9180 9784 1214
Size: 456 564 969 135 135 604 4215
Fragments arranged by size:
4215 1987 1152 969 789 684 604 564
457 456 435 171 135 135 32
DraIII CAC_nnn'GTG
Cuts at: 7498 7498
Size: 12785
DrdI GACnn_nn'nnGTC
Cuts at: 10491 11349 10491
Size: 858 11927
EaeI y'GGCC_r
Cuts at: 4485 5892 5904 5922 6065 6716 9587 10370 10544
Size: 1407 12 18 143 651 2871 783 174
Cuts at: 10544 10935 10962 12307 4485
Size: 391 27 1345 4963
Fragments arranged by size:
4963 2871 1407 1345 783 651 391 174
143 27 18 12
EagI C'GGCC_G
Cuts at: 6716 10370 6716
Size: 3654 9131
EarI CTCTTCn'nnn_
Cuts at: 438 908 3644 5052 5229 5403 5607 6330 6622
Size: 470 2736 1408 177 174 204 723 292
Cuts at: 6622 7010 8111 8264 9024 10808 11018 438
Size: 388 1101 153 760 1784 210 2205
Fragments arranged by size:
2736 2205 1784 1408 1101 760 723 470
388 292 210 204 177 174 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9357 9524 10993 11303 11449 12595 9357
Size: 167 1469 310 146 1146 9547
Fragments arranged by size:
9547 1469 1146 310 167 146
EcoICRI GAG'CTC
Cuts at: 1039 4260 4592 9797 1039
Size: 3221 332 5205 4027
Fragments arranged by size:
5205 4027 3221 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 889 1393 1837 2231 2344 2711 3305 4667
Size: 117 504 444 394 113 367 594 1362
Cuts at: 4667 4856 5033 5210 5546 5954 6050 6412 6524
Size: 189 177 177 336 408 96 362 112
Cuts at: 6524 6638 6897 6959 7253 7568 7856 7997 8477
Size: 114 259 62 294 315 288 141 480
Cuts at: 8477 8507 8702 8753 8903 9232 9378 9431 9655
Size: 30 195 51 150 329 146 53 224
Cuts at: 9655 10610 11042 11789 12057 772
Size: 955 432 747 268 1500
Fragments arranged by size:
1500 1362 955 747 594 504 480 444
432 408 394 367 362 336 329 315
294 288 268 259 224 195 189 177
177 150 146 141 117 114 113 112
96 62 53 51 30
EcoO109I rG'GnC_Cy
Cuts at: 2386 2805 9924 2386
Size: 419 7119 5247
Fragments arranged by size:
7119 5247 419
EcoRI G'AATT_C
Cuts at: 717 1440 2529 2963 3471 3685 6508 9435 9789
Size: 723 1089 434 508 214 2823 2927 354
Cuts at: 9789 717
Size: 3713
Fragments arranged by size:
3713 2927 2823 1089 723 508 434 354
214
EcoRV GAT'ATC
Cuts at: 2671 7860 8835 9486 2671
Size: 5189 975 651 5970
Fragments arranged by size:
5970 5189 975 651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 3176 4000 4469 5954 6196 10804 2359
Size: 817 824 469 1485 242 4608 4340
Fragments arranged by size:
4608 4340 1485 824 817 469 242
FspI TGC'GCA
Cuts at: 10566 10566
Size: 12785
HaeII r_GCGC'y
Cuts at: 10219 10467 11489 12468 10219
Size: 248 1022 979 10536
Fragments arranged by size:
10536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 2910 2942 3054 3086 3712 3744 4510
Size: 32 1953 32 112 32 626 32 766
Cuts at: 4510 4542 5762 5794 6651 6683 6858 6890 6979
Size: 32 1220 32 857 32 175 32 89
Cuts at: 6979 7011 7443 7475 7972 8004 8353 8385 9470
Size: 32 432 32 497 32 349 32 1085
Cuts at: 9470 9502 10627 10657 10659 10689 925
Size: 32 1125 30 2 30 3021
Fragments arranged by size:
3021 1953 1220 1125 1085 857 766 626
497 432 349 175 112 89 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 5759 6896 8567 9824 5759
Size: 1137 1671 1257 8720
Fragments arranged by size:
8720 1671 1257 1137
HindIII A'AGCT_T
Cuts at: 4469 5651 4469
Size: 1182 11603
KasI G'GCGC_C
Cuts at: 10215 10463 10215
Size: 248 12537
KpnI G_GTAC'C
Cuts at: 8470 9805 8470
Size: 1335 11450
MluI A'CGCG_T
Cuts at: 12218 12218
Size: 12785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4678 7933 8050 8095 8742 8952 11456 11640 4678
Size: 3255 117 45 647 210 2504 184 5823
Fragments arranged by size:
5823 3255 2504 647 210 184 117 45
MscI TGG'CCA
Cuts at: 4487 5894 5924 6067 9589 10546 4487
Size: 1407 30 143 3522 957 6726
Fragments arranged by size:
6726 3522 1407 957 143 30
MslI CAynn'nnrTG
Cuts at: 268 671 2511 2849 3596 5782 6423 6774 10774
Size: 403 1840 338 747 2186 641 351 4000
Cuts at: 10774 10901 268
Size: 127 2152
Fragments arranged by size:
4000 2186 2152 1840 747 641 403 351
338 127
MspA1I CmG'CkG
Cuts at: 1868 4187 6222 6537 8032 8146 8740 9896 10210
Size: 2319 2035 315 1495 114 594 1156 314
Cuts at: 10210 10570 11583 11828 1868
Size: 360 1013 245 2825
Fragments arranged by size:
2825 2319 2035 1495 1156 1013 594 360
315 314 245 114
NaeI GCC'GGC
Cuts at: 10966 10966
Size: 12785
NarI GG'CG_CC
Cuts at: 10216 10464 10216
Size: 248 12537
NcoI C'CATG_G
Cuts at: 3510 3591 4046 9584 3510
Size: 81 455 5538 6711
Fragments arranged by size:
6711 5538 455 81
NdeI CA'TA_TG
Cuts at: 253 253
Size: 12785
NgoMIV G'CCGG_C
Cuts at: 10964 10964
Size: 12785
NsiI A_TGCA'T
Cuts at: 983 983
Size: 12785
NspI r_CATG'y
Cuts at: 981 985 1179 3910 4571 4725 5698 6841 7328
Size: 4 194 2731 661 154 973 1143 487
Cuts at: 7328 9262 9667 9838 10338 11245 981
Size: 1934 405 171 500 907 2521
Fragments arranged by size:
2731 2521 1934 1143 973 907 661 500
487 405 194 171 154 4
PasI CC'CwG_GG
Cuts at: 2975 2975
Size: 12785
PciI A'CATG_T
Cuts at: 1175 4721 9258 11241 1175
Size: 3546 4537 1983 2719
Fragments arranged by size:
4537 3546 2719 1983
PflMI CCAn_nnn'nTGG
Cuts at: 2815 3370 3737 3880 5425 2815
Size: 555 367 143 1545 10175
Fragments arranged by size:
10175 1545 555 367 143
PmeI GTTT'AAAC
Cuts at: 9784 9784
Size: 12785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4897 4929 5074 5106 5251 5283 11924 11956 4897
Size: 32 145 32 145 32 6641 32 5726
Fragments arranged by size:
6641 5726 145 145 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 2386
Size: 12785
PshAI GACnn'nnGTC
Cuts at: 180 180
Size: 12785
PsiI TTA'TAA
Cuts at: 503 9628 503
Size: 9125 3660
PspOMI G'GGCC_C
Cuts at: 2805 6763 2805
Size: 3958 8827
PstI C_TGCA'G
Cuts at: 3090 3282 5031 5208 9832 10517 3090
Size: 192 1749 177 4624 685 5358
Fragments arranged by size:
5358 4624 1749 685 192 177
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12785
PvuII CAG'CTG
Cuts at: 4187 8146 8740 10210 10570 4187
Size: 3959 594 1470 360 6402
Fragments arranged by size:
6402 3959 1470 594 360
RsrII CG'GwC_CG
Cuts at: 10980 10980
Size: 12785
SacI G_AGCT'C
Cuts at: 1041 4262 4594 9799 1041
Size: 3221 332 5205 4027
Fragments arranged by size:
5205 4027 3221 332
SalI G'TCGA_C
Cuts at: 9822 9822
Size: 12785
SapI GCTCTTCn'nnn_
Cuts at: 438 908 10808 11018 438
Size: 470 9900 210 2205
Fragments arranged by size:
9900 2205 470 210
SbfI CC_TGCA'GG
Cuts at: 9832 9832
Size: 12785
ScaI AGT'ACT
Cuts at: 6347 7033 12302 6347
Size: 686 5269 6830
Fragments arranged by size:
6830 5269 686
SexAI A'CCwGG_T
Cuts at: 9035 9035
Size: 12785
SfcI C'TryA_G
Cuts at: 33 2373 3086 3101 3278 3348 3629 5027 5204
Size: 2340 713 15 177 70 281 1398 177
Cuts at: 5204 7125 7381 7511 8325 8555 9828 10513 11506
Size: 1921 256 130 814 230 1273 685 993
Cuts at: 11506 11697 33
Size: 191 1121
Fragments arranged by size:
2340 1921 1398 1273 1121 993 814 713
685 281 256 230 191 177 177 130
70 15
SfoI GGC'GCC
Cuts at: 10217 10465 10217
Size: 248 12537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12785
SmaI CCC'GGG
Cuts at: 9807 9807
Size: 12785
SmlI C'TyrA_G
Cuts at: 739 1188 1512 7007 9940 10855 11347 11609 739
Size: 449 324 5495 2933 915 492 262 1915
Fragments arranged by size:
5495 2933 1915 915 492 449 324 262
SpeI A'CTAG_T
Cuts at: 67 7786 67
Size: 7719 5066
SphI G_CATG'C
Cuts at: 985 3910 9838 10338 985
Size: 2925 5928 500 3432
Fragments arranged by size:
5928 3432 2925 500
StyI C'CwwG_G
Cuts at: 1572 3510 3591 4046 8201 8601 9474 9584 9919
Size: 1938 81 455 4155 400 873 110 335
Cuts at: 9919 10273 1572
Size: 354 4084
Fragments arranged by size:
4155 4084 1938 873 455 400 354 335
110 81
SwaI ATTT'AAAT
Cuts at: 4479 5802 4479
Size: 1323 11462
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 913 1169 3555 7460 10368 10501 10960 11169 913
Size: 256 2386 3905 2908 133 459 209 2529
Fragments arranged by size:
3905 2908 2529 2386 459 256 209 133
TatI w'GTAC_w
Cuts at: 256 729 1678 1714 1833 5481 6345 6834 7031
Size: 473 949 36 119 3648 864 489 197
Cuts at: 7031 12300 256
Size: 5269 741
Fragments arranged by size:
5269 3648 949 864 741 489 473 197
119 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 708 740 1615 1647 3042 3074 3732 3764 4537
Size: 32 875 32 1395 32 658 32 773
Cuts at: 4537 4569 4729 4761 4918 4950 5095 5127 5272
Size: 32 160 32 157 32 145 32 145
Cuts at: 5272 5304 5600 5632 7311 7343 8726 8758 12302
Size: 32 296 32 1679 32 1383 32 3544
Cuts at: 12302 12334 708
Size: 32 1159
Fragments arranged by size:
3544 1679 1395 1383 1159 875 773 658
296 160 157 145 145 32 32 32
32 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 10582 10582
Size: 12785
XbaI T'CTAG_A
Cuts at: 522 9816 522
Size: 9294 3491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1807 10280 1807
Size: 8473 4312
XmaI C'CCGG_G
Cuts at: 9805 9805
Size: 12785
XmnI GAAnn'nnTTC
Cuts at: 193 518 1565 1850 5981 6206 6643 8363 8819
Size: 325 1047 285 4131 225 437 1720 456
Cuts at: 8819 193
Size: 4159
Fragments arranged by size:
4159 4131 1720 1047 456 437 325 285
225
ZraI GAC'GTC
Cuts at: 600 600
Size: 12785
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AhdI AleI AloI AscI AvrII BaeI BglI
BmtI BsiWI BssHII BstEII BstZ17I EcoNI FseI FspAI
HpaI MfeI NheI NotI NruI PacI PfoI PmlI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI XhoI