Restriction map for
pF1KE2359
(Circular) (Six-base) MAPSORT of: pF1KE2359.seq Check: 8525 from: 1 to: 4274
pF1KE2359 4274 bp
With 182 enzymes: SgfI *
June 23, 2011 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 700 972 700
Size: 272 4002
AatII G_ACGT'C
Cuts at: 410 410
Size: 4274
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4274
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 2531 3278 3546 2099
Size: 432 747 268 2827
Fragments arranged by size:
2827 747 432 268
AfeI AGC'GCT
Cuts at: 1105 1105
Size: 4274
AflIII A'CryG_T
Cuts at: 1173 1626 2730 3707 1173
Size: 453 1104 977 1740
Fragments arranged by size:
1740 1104 977 453
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 1579 3146 1579
Size: 1567 2707
ApaLI G'TGCA_C
Cuts at: 3044 3044
Size: 4274
ApoI r'AATT_y
Cuts at: 1278 3627 3883 1278
Size: 2349 256 1669
Fragments arranged by size:
2349 1669 256
AseI AT'TA_AT
Cuts at: 20 526 1160 20
Size: 506 634 3134
Fragments arranged by size:
3134 634 506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 1219 1294 1219
Size: 75 4199
BamHI G'GATC_C
Cuts at: 951 1165 1299 951
Size: 214 134 3926
Fragments arranged by size:
3926 214 134
BanI G'GyrC_C
Cuts at: 337 924 1290 1704 1952 1987 337
Size: 587 366 414 248 35 2624
Fragments arranged by size:
2624 587 414 366 248 35
BanII G_rGCy'C
Cuts at: 1288 2318 1288
Size: 1030 3244
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4026
BbsI GAAGACnn'nnnn_
Cuts at: 654 1119 654
Size: 465 3809
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2933 4274 2172
Size: 761 1341 2172
Fragments arranged by size:
2172 1341 761
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 1386 1386
Size: 4274
Bme1580I G_kGCm'C
Cuts at: 927 1899 1992 3048 927
Size: 972 93 1056 2153
Fragments arranged by size:
2153 1056 972 93
BmgBI CAC'GTC
Cuts at: 1069 1069
Size: 4274
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1567 1902 4046 1486
Size: 81 335 2144 1714
Fragments arranged by size:
2144 1714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 842 2641 842
Size: 1799 2475
Bpu10I CC'TnA_GC
Cuts at: 256 764 256
Size: 508 3766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2329 2821 3119 1450
Size: 879 492 298 2605
Fragments arranged by size:
2605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 862 3978 862
Size: 3116 1158
BsaAI yAC'GTr
Cuts at: 653 1627 653
Size: 974 3300
BsaBI GATnn'nnATC
Cuts at: 6 205 1788 3825 6
Size: 199 1583 2037 455
Fragments arranged by size:
2037 1583 455 199
BsaHI Gr'CG_yC
Cuts at: 407 1705 1953 2655 4151 407
Size: 1298 248 702 1496 530
Fragments arranged by size:
1496 1298 702 530 248
BsaWI w'CCGG_w
Cuts at: 1463 1984 2662 2936 3083 3494 1463
Size: 521 678 274 147 411 2243
Fragments arranged by size:
2243 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 715 801 154
Size: 561 86 3627
Fragments arranged by size:
3627 561 86
BseYI C'CCAG_C
Cuts at: 242 531 542 920 1699 2457 3034 3530 242
Size: 289 11 378 779 758 577 496 986
Fragments arranged by size:
986 779 758 577 496 378 289 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1180
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3070 104
Size: 1712 46 1208 1308
Fragments arranged by size:
1712 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1288 2066 2256 3048 1288
Size: 778 190 792 2514
Fragments arranged by size:
2514 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 927 1288 1899 1992 2066 2256 2318 3048 927
Size: 361 611 93 74 190 62 730 2153
Fragments arranged by size:
2153 730 611 361 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3494 3494
Size: 4274
BspHI T'CATG_A
Cuts at: 3450 3450
Size: 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 227 700 972 1324 1538 1840 2221 227
Size: 473 272 352 214 302 381 2280
Fragments arranged by size:
2280 473 381 352 302 272 214
BsrBI CCG'CTC
Cuts at: 50 2566 2620 4104 4269 50
Size: 2516 54 1484 165 55
Fragments arranged by size:
2516 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2186 2186
Size: 4274
BsrFI r'CCGG_y
Cuts at: 2272 2453 2662 2272
Size: 181 209 3884
Fragments arranged by size:
3884 209 181
BssSI C'ACGA_G
Cuts at: 2545 2903 2545
Size: 358 3916
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 1651
Size: 4274
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstXI CCAn_nnnn'nTGG
Cuts at: 348 948 348
Size: 600 3674
BstYI r'GATC_y
Cuts at: 1 303 782 951 1165 1299 1766 1789 2124
Size: 302 479 169 214 134 467 23 335
Cuts at: 2124 2370 3382 3468 3480 3826 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 479 467 449 346 335 302 246
214 169 134 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 504 838 2224 2405 2568 504
Size: 334 1386 181 163 2210
Fragments arranged by size:
2210 1386 334 181 163
BtsI GCAGTG_nn'
Cuts at: 1517 1517
Size: 4274
ClaI AT'CG_AT
Cuts at: 427 427
Size: 4274
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 970 1980 2838 970
Size: 1010 858 2406
Fragments arranged by size:
2406 1010 858
EaeI y'GGCC_r
Cuts at: 203 1859 2033 2424 2451 3796 203
Size: 1656 174 391 27 1345 681
Fragments arranged by size:
1656 1345 681 391 174 27
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4274
EarI CTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2482 2792 2938 4084 2482
Size: 310 146 1146 2672
Fragments arranged by size:
2672 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1286 1286
Size: 4274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 2531 3278 3546 2099
Size: 432 747 268 2827
Fragments arranged by size:
2827 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1413 1413
Size: 4274
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 4274
FspI TGC'GCA
Cuts at: 517 2055 517
Size: 1538 2736
HaeII r_GCGC'y
Cuts at: 1107 1708 1956 2978 3957 1107
Size: 601 248 1022 979 1424
Fragments arranged by size:
1424 1022 979 601 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 495 527 1234 1266 2116 2146 2148 2178 495
Size: 32 707 32 850 30 2 30 2591
Fragments arranged by size:
2591 850 707 32 32 30 30 2
HincII GTy'rAC
Cuts at: 672 1313 672
Size: 641 3633
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4026
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3129 2945
Size: 184 4090
MscI TGG'CCA
Cuts at: 205 2035 205
Size: 1830 2444
MslI CAynn'nnrTG
Cuts at: 898 2263 2390 898
Size: 1365 127 2782
Fragments arranged by size:
2782 1365 127
MspA1I CmG'CkG
Cuts at: 1385 1699 2059 3072 3317 1385
Size: 314 360 1013 245 2342
Fragments arranged by size:
2342 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4274
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4026
NcoI C'CATG_G
Cuts at: 107 107
Size: 4274
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4274
NspI r_CATG'y
Cuts at: 775 1177 1327 1827 2734 775
Size: 402 150 500 907 2315
Fragments arranged by size:
2315 907 500 402 150
PasI CC'CwG_GG
Cuts at: 976 976
Size: 4274
PciI A'CATG_T
Cuts at: 1173 2730 1173
Size: 1557 2717
PflMI CCAn_nnn'nTGG
Cuts at: 486 486
Size: 4274
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 3413 3445 631
Size: 32 2750 32 1460
Fragments arranged by size:
2750 1460 32 32
PstI C_TGCA'G
Cuts at: 1321 2006 1321
Size: 685 3589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 1699 2059 1699
Size: 360 3914
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 1288 1288
Size: 4274
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 492 1317 2002 2995 3186 33
Size: 459 825 685 993 191 1121
Fragments arranged by size:
1121 993 825 685 459 191
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4026
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4274
SmlI C'TyrA_G
Cuts at: 1219 1429 2344 2836 3098 1219
Size: 210 915 492 262 2395
Fragments arranged by size:
2395 915 492 262 210
SnaBI TAC'GTA
Cuts at: 653 653
Size: 4274
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 775 1327 1827 775
Size: 552 500 3222
Fragments arranged by size:
3222 552 500
SspI AAT'ATT
Cuts at: 945 945
Size: 4274
StuI AGG'CCT
Cuts at: 825 825
Size: 4274
StyI C'CwwG_G
Cuts at: 107 1408 1762 107
Size: 1301 354 2619
Fragments arranged by size:
2619 1301 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1857 1990 2449 2658 1857
Size: 133 459 209 3473
Fragments arranged by size:
3473 459 209 133
TatI w'GTAC_w
Cuts at: 1176 3789 1176
Size: 2613 1661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4242
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1769 1769
Size: 4274
XhoI C'TCGA_G
Cuts at: 1219 1219
Size: 4274
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4274
XmnI GAAnn'nnTTC
Cuts at: 1116 1116
Size: 4274
ZraI GAC'GTC
Cuts at: 408 408
Size: 4274
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BpmI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SwaI