Restriction map for
pF1KE2363
(Circular) (Six-base) MAPSORT of: pF1KE2363.seq Check: 7010 from: 1 to: 4664
pF1KE2363 4664 bp
With 182 enzymes: SgfI *
July 21, 2011 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 832 1546 832
Size: 714 3950
AccI GT'mk_AC
Cuts at: 458 1702 458
Size: 1244 3420
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 2489 2921 3668 3936 335
Size: 2154 432 747 268 1063
Fragments arranged by size:
2154 1063 747 432 268
AflII C'TTAA_G
Cuts at: 1603 1603
Size: 4664
AflIII A'CryG_T
Cuts at: 2016 3120 4097 2016
Size: 1104 977 2583
Fragments arranged by size:
2583 1104 977
AgeI A'CCGG_T
Cuts at: 889 3052 889
Size: 2163 2501
AleI CACnn'nnGTG
Cuts at: 901 901
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 1377 1969 3536 1377
Size: 592 1567 2505
Fragments arranged by size:
2505 1567 592
ApaLI G'TGCA_C
Cuts at: 3434 3434
Size: 4664
ApoI r'AATT_y
Cuts at: 1668 4017 4273 1668
Size: 2349 256 2059
Fragments arranged by size:
2349 2059 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 356 472 1114 1457 1684 356
Size: 116 642 343 227 3336
Fragments arranged by size:
3336 642 343 227 116
AvrII C'CTAG_G
Cuts at: 1218 1218
Size: 4664
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 123 386 1680 2094 2342 2377 123
Size: 263 1294 414 248 35 2410
Fragments arranged by size:
2410 1294 414 263 248 35
BanII G_rGCy'C
Cuts at: 632 979 1678 2708 632
Size: 347 699 1030 2588
Fragments arranged by size:
2588 1030 699 347
BbeI G_GCGC'C
Cuts at: 390 2098 2346 390
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
BbsI GAAGACnn'nnnn_
Cuts at: 268 268
Size: 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4630
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3323 4664 2562
Size: 761 1341 2562
Fragments arranged by size:
2562 1341 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BglI GCCn_nnn'nGGC
Cuts at: 146 146
Size: 4664
BglII A'GATC_T
Cuts at: 1355 2179 4216 1355
Size: 824 2037 1803
Fragments arranged by size:
2037 1803 824
BlpI GC'TnA_GC
Cuts at: 136 1776 136
Size: 1640 3024
Bme1580I G_kGCm'C
Cuts at: 308 2289 2382 3438 308
Size: 1981 93 1056 1534
Fragments arranged by size:
1981 1534 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1876 1957 2292 4436 1876
Size: 81 335 2144 2104
Fragments arranged by size:
2144 2104 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 979 1480 127
Size: 852 501 3311
Fragments arranged by size:
3311 852 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1840 2719 3211 3509 928
Size: 912 879 492 298 2083
Fragments arranged by size:
2083 912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 469 4368 469
Size: 3899 765
BsaAI yAC'GTr
Cuts at: 309 2017 309
Size: 1708 2956
BsaBI GATnn'nnATC
Cuts at: 6 335 2178 4215 6
Size: 329 1843 2037 455
Fragments arranged by size:
2037 1843 455 329
BsaHI Gr'CG_yC
Cuts at: 387 2095 2343 3045 4541 387
Size: 1708 248 702 1496 510
Fragments arranged by size:
1708 1496 702 510 248
BsaWI w'CCGG_w
Cuts at: 889 1853 2374 3052 3326 3473 3884 889
Size: 964 521 678 274 147 411 1669
Fragments arranged by size:
1669 964 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 156 1249 1366 1414 156
Size: 1093 117 48 3406
Fragments arranged by size:
3406 1093 117 48
BseYI C'CCAG_C
Cuts at: 560 857 963 979 1175 1587 2089 2847 3424
Size: 297 106 16 196 412 502 758 577
Cuts at: 3424 3920 560
Size: 496 1304
Fragments arranged by size:
1304 758 577 502 496 412 297 196
106 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 1390 1345
Size: 45 4619
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3460 104
Size: 2102 46 1208 1308
Fragments arranged by size:
2102 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1678 2456 2646 3438 1678
Size: 778 190 792 2904
Fragments arranged by size:
2904 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 308 632 979 1678 2289 2382 2456 2646 2708
Size: 324 347 699 611 93 74 190 62
Cuts at: 2708 3438 308
Size: 730 1534
Fragments arranged by size:
1534 730 699 611 347 324 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3884 3884
Size: 4664
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1546 1714 1928 2230 2611 832
Size: 714 168 214 302 381 2885
Fragments arranged by size:
2885 714 381 302 214 168
BsrBI CCG'CTC
Cuts at: 50 408 447 2956 3010 4494 4659 50
Size: 358 39 2509 54 1484 165 55
Fragments arranged by size:
2509 1484 358 165 55 54 39
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 413 889 2662 2843 3052 413
Size: 476 1773 181 209 2025
Fragments arranged by size:
2025 1773 476 209 181
BssSI C'ACGA_G
Cuts at: 2935 3293 2935
Size: 358 4306
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 319 1629 319
Size: 1310 3354
BstXI CCAn_nnnn'nTGG
Cuts at: 594 903 594
Size: 309 4355
BstYI r'GATC_y
Cuts at: 1 1355 1461 1689 2156 2179 2514 2760 3772
Size: 1354 106 228 467 23 335 246 1012
Cuts at: 3772 3858 3870 4216 1
Size: 86 12 346 449
Fragments arranged by size:
1354 1012 467 449 346 335 246 228
106 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1187 1187
Size: 4664
BtgI C'CryG_G
Cuts at: 382 593 658 1016 1124 382
Size: 211 65 358 108 3922
Fragments arranged by size:
3922 358 211 108 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 837 2614 2795 2958 837
Size: 1777 181 163 2543
Fragments arranged by size:
2543 1777 181 163
BtsI GCAGTG_nn'
Cuts at: 1030 1907 1030
Size: 877 3787
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 600 645 848 992 1236 2249 2423 2814 2841
Size: 45 203 144 244 1013 174 391 27
Cuts at: 2841 4186 600
Size: 1345 1078
Fragments arranged by size:
1345 1078 1013 391 244 203 174 144
45 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 1283 2872 3182 3328 4474 358
Size: 925 1589 310 146 1146 548
Fragments arranged by size:
1589 1146 925 548 310 146
EcoICRI GAG'CTC
Cuts at: 1676 1676
Size: 4664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 335 979 1480 2489 2921 3668 3936 127
Size: 208 644 501 1009 432 747 268 855
Fragments arranged by size:
1009 855 747 644 501 432 268 208
EcoNI CCTnn'n_nnAGG
Cuts at: 781 781
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 377 1183 1803 377
Size: 806 620 3238
Fragments arranged by size:
3238 806 620
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 282 2683 282
Size: 2401 2263
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 390 2098 2346 3368 4347 390
Size: 1708 248 1022 979 707
Fragments arranged by size:
1708 1022 979 707 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2506 2536 2538 2568 2506
Size: 30 2 30 4602
Fragments arranged by size:
4602 30 30 2
HincII GTy'rAC
Cuts at: 279 873 1703 279
Size: 594 830 3240
Fragments arranged by size:
3240 830 594
KasI G'GCGC_C
Cuts at: 386 2094 2342 386
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 667 962 3335 3519 667
Size: 295 2373 184 1812
Fragments arranged by size:
2373 1812 295 184
MscI TGG'CCA
Cuts at: 602 647 1238 2425 602
Size: 45 591 1187 2841
Fragments arranged by size:
2841 1187 591 45
MslI CAynn'nnrTG
Cuts at: 538 592 866 901 2653 2780 538
Size: 54 274 35 1752 127 2422
Fragments arranged by size:
2422 1752 274 127 54 35
MspA1I CmG'CkG
Cuts at: 222 928 1775 2089 2449 3462 3707 222
Size: 706 847 314 360 1013 245 1179
Fragments arranged by size:
1179 1013 847 706 360 314 245
NaeI GCC'GGC
Cuts at: 415 2845 415
Size: 2430 2234
NarI GG'CG_CC
Cuts at: 387 2095 2343 387
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
NcoI C'CATG_G
Cuts at: 382 593 382
Size: 211 4453
NgoMIV G'CCGG_C
Cuts at: 413 2843 413
Size: 2430 2234
NspI r_CATG'y
Cuts at: 1015 1717 2217 3124 1015
Size: 702 500 907 2555
Fragments arranged by size:
2555 907 702 500
PasI CC'CwG_GG
Cuts at: 802 802
Size: 4664
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PflMI CCAn_nnn'nTGG
Cuts at: 864 864
Size: 4664
PfoI T'CCnGG_A
Cuts at: 1075 1075
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PmlI CAC'GTG
Cuts at: 309 309
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 377 377
Size: 4664
PstI C_TGCA'G
Cuts at: 1711 2396 1711
Size: 685 3979
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 222 928 2089 2449 222
Size: 706 1161 360 2437
Fragments arranged by size:
2437 1161 706 360
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1678 1678
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SanDI GG'GwC_CC
Cuts at: 377 377
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SexAI A'CCwGG_T
Cuts at: 1426 1426
Size: 4664
SfcI C'TryA_G
Cuts at: 33 1707 2392 3385 3576 33
Size: 1674 685 993 191 1121
Fragments arranged by size:
1674 1121 993 685 191
SfoI GGC'GCC
Cuts at: 388 2096 2344 388
Size: 1708 248 2708
Fragments arranged by size:
2708 1708 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 358 1459 1686 358
Size: 1101 227 3336
Fragments arranged by size:
3336 1101 227
SmlI C'TyrA_G
Cuts at: 472 943 1114 1603 1819 2734 3226 3488 472
Size: 471 171 489 216 915 492 262 1648
Fragments arranged by size:
1648 915 492 489 471 262 216 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1015 1717 2217 1015
Size: 702 500 3462
Fragments arranged by size:
3462 702 500
StuI AGG'CCT
Cuts at: 1609 1609
Size: 4664
StyI C'CwwG_G
Cuts at: 382 479 515 593 671 1218 1798 2152 382
Size: 97 36 78 78 547 580 354 2894
Fragments arranged by size:
2894 580 547 354 97 78 78 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 955 1189 1501 1579 2247 2380 2839 3048 955
Size: 234 312 78 668 133 459 209 2571
Fragments arranged by size:
2571 668 459 312 234 209 133 78
TatI w'GTAC_w
Cuts at: 1478 4179 1478
Size: 2701 1963
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4181 4213 4181
Size: 32 4632
Tth111I GACn'n_nGTC
Cuts at: 2461 2461
Size: 4664
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 925 987 1434 1549 2159 925
Size: 62 447 115 610 3430
Fragments arranged by size:
3430 610 447 115 62
XhoI C'TCGA_G
Cuts at: 472 1114 472
Size: 642 4022
XmaI C'CCGG_G
Cuts at: 356 1457 1684 356
Size: 1101 227 3336
Fragments arranged by size:
3336 1101 227
XmnI GAAnn'nnTTC
Cuts at: 265 496 265
Size: 231 4433
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI ZraI