Restriction map for
pF1KE2368
(Circular) (Six-base) MAPSORT of: pF1KE2368.seq Check: 5871 from: 1 to: 9158
pF1KE2368 9158 bp
With 182 enzymes: SgfI *
September 22, 2011 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4335 5858 4335
Size: 1523 7635
AatII G_ACGT'C
Cuts at: 3947 4700 3947
Size: 753 8405
AccI GT'mk_AC
Cuts at: 3941 6196 3941
Size: 2255 6903
Acc65I G'GTAC_C
Cuts at: 4328 6174 4328
Size: 1846 7312
AclI AA'CG_TT
Cuts at: 8998 8998
Size: 9158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1541 1745 2916 3632 4131 5412 5737 6983
Size: 706 204 1171 716 499 1281 325 1246
Cuts at: 6983 7415 8162 8430 835
Size: 432 747 268 1563
Fragments arranged by size:
1563 1281 1246 1171 747 716 706 499
432 325 268 204
AfeI AGC'GCT
Cuts at: 965 1405 2185 3767 965
Size: 440 780 1582 6356
Fragments arranged by size:
6356 1582 780 440
AflIII A'CryG_T
Cuts at: 659 1108 4439 5174 5399 6510 7614 8591 659
Size: 449 3331 735 225 1111 1104 977 1226
Fragments arranged by size:
3331 1226 1111 1104 977 735 449 225
AgeI A'CCGG_T
Cuts at: 1124 7546 1124
Size: 6422 2736
AhdI GACnn_n'nnGTC
Cuts at: 6141 6141
Size: 9158
AleI CACnn'nnGTG
Cuts at: 4234 4234
Size: 9158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5866 5866
Size: 9158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1836 1868 5722 5754 1836
Size: 32 3854 32 5240
Fragments arranged by size:
5240 3854 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1564 2034 2956 3386 3406 6463 8030 1564
Size: 470 922 430 20 3057 1567 2692
Fragments arranged by size:
3057 2692 1567 922 470 430 20
ApaI G_GGCC'C
Cuts at: 786 834 2027 2094 3997 786
Size: 48 1193 67 1903 5947
Fragments arranged by size:
5947 1903 1193 67 48
ApaLI G'TGCA_C
Cuts at: 522 4022 7928 522
Size: 3500 3906 1752
Fragments arranged by size:
3906 3500 1752
ApoI r'AATT_y
Cuts at: 730 2338 5503 6162 8511 8767 730
Size: 1608 3165 659 2349 256 1121
Fragments arranged by size:
3165 2349 1608 1121 659 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9158
AvaI C'yCGr_G
Cuts at: 128 247 641 3223 5318 6178 128
Size: 119 394 2582 2095 860 3108
Fragments arranged by size:
3108 2582 2095 860 394 119
BamHI G'GATC_C
Cuts at: 3228 5030 6183 3228
Size: 1802 1153 6203
Fragments arranged by size:
6203 1802 1153
BanI G'GyrC_C
Cuts at: 133 973 1063 1127 1794 3162 3217 4090 4328
Size: 840 90 64 667 1368 55 873 238
Cuts at: 4328 5846 6174 6588 6836 6871 133
Size: 1518 328 414 248 35 2420
Fragments arranged by size:
2420 1518 1368 873 840 667 414 328
248 238 90 64 55 35
BanII G_rGCy'C
Cuts at: 332 344 786 834 2027 2094 3239 3554 3997
Size: 12 442 48 1193 67 1145 315 443
Cuts at: 3997 4044 4612 5338 5459 5777 6172 7202 332
Size: 47 568 726 121 318 395 1030 2288
Fragments arranged by size:
2288 1193 1145 1030 726 568 443 442
395 318 315 121 67 48 47 12
BbeI G_GCGC'C
Cuts at: 4094 6592 6840 4094
Size: 2498 248 6412
Fragments arranged by size:
6412 2498 248
BbsI GAAGACnn'nnnn_
Cuts at: 823 873 1547 2364 2544 2853 3808 3843 823
Size: 50 674 817 180 309 955 35 6138
Fragments arranged by size:
6138 955 817 674 309 180 50 35
BbvCI CC'TCA_GC
Cuts at: 889 1651 2095 2710 5254 889
Size: 762 444 615 2544 4793
Fragments arranged by size:
4793 2544 762 615 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 632 666 1162 1196 3412 3446 5905 5939 632
Size: 34 496 34 2216 34 2459 34 3851
Fragments arranged by size:
3851 2459 2216 496 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1461 7056 7817 9158 1461
Size: 5595 761 1341 1461
Fragments arranged by size:
5595 1461 1341 761
BclI T'GATC_A
Cuts at: 6678 6678
Size: 9158
BglI GCCn_nnn'nGGC
Cuts at: 2022 2742 2022
Size: 720 8438
BglII A'GATC_T
Cuts at: 303 1268 5492 5762 6102 6673 8710 303
Size: 965 4224 270 340 571 2037 751
Fragments arranged by size:
4224 2037 965 751 571 340 270
BlpI GC'TnA_GC
Cuts at: 2923 3548 6270 2923
Size: 625 2722 5811
Fragments arranged by size:
5811 2722 625
Bme1580I G_kGCm'C
Cuts at: 136 526 786 834 1799 1824 2027 2094 2622
Size: 390 260 48 965 25 203 67 528
Cuts at: 2622 3220 3251 3313 3997 4026 4921 6783 6876
Size: 598 31 62 684 29 895 1862 93
Cuts at: 6876 7932 136
Size: 1056 1362
Fragments arranged by size:
1862 1362 1056 965 895 684 598 528
390 260 203 93 67 62 48 31
29 25
BmgBI CAC'GTC
Cuts at: 5231 5231
Size: 9158
BmrI ACTGGGnnnn_n'
Cuts at: 1504 1829 1927 2632 5282 6370 6451 6786 8930
Size: 325 98 705 2650 1088 81 335 2144
Cuts at: 8930 1504
Size: 1732
Fragments arranged by size:
2650 2144 1732 1088 705 335 325 98
81
BmtI G_CTAG'C
Cuts at: 2647 2647
Size: 9158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5959 5988 7525 5959
Size: 29 1537 7592
Fragments arranged by size:
7592 1537 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 2335 2968 3408 3577 3880 4380 5440 5979
Size: 1968 633 440 169 303 500 1060 539
Cuts at: 5979 6052 367
Size: 73 3473
Fragments arranged by size:
3473 1968 1060 633 539 500 440 303
169 73
Bpu10I CC'TnA_GC
Cuts at: 889 1651 2095 2479 2710 5254 5930 889
Size: 762 444 384 231 2544 676 4117
Fragments arranged by size:
4117 2544 762 676 444 384 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1414 3841 3856 6334 7213 7705 8003 1039
Size: 375 2427 15 2478 879 492 298 2194
Fragments arranged by size:
2478 2427 2194 879 492 375 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 1378 1706 1914 2803 3061 3487 8862 1378
Size: 328 208 889 258 426 5375 1674
Fragments arranged by size:
5375 1674 889 426 328 258 208
BsaAI yAC'GTr
Cuts at: 5955 6511 5955
Size: 556 8602
BsaBI GATnn'nnATC
Cuts at: 6 2221 6672 8709 6
Size: 2215 4451 2037 455
Fragments arranged by size:
4451 2215 2037 455
BsaHI Gr'CG_yC
Cuts at: 3944 4091 4697 5711 6589 6837 7539 9035 3944
Size: 147 606 1014 878 248 702 1496 4067
Fragments arranged by size:
4067 1496 1014 878 702 606 248 147
BsaWI w'CCGG_w
Cuts at: 416 467 1113 1124 3352 3372 4273 6126 6347
Size: 51 646 11 2228 20 901 1853 221
Cuts at: 6347 6868 7546 7820 7967 8378 416
Size: 521 678 274 147 411 1196
Fragments arranged by size:
2228 1853 1196 901 678 646 521 411
274 221 147 51 20 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1836 1866 3307 3337 3496 3526 4939 4969 5722
Size: 30 1441 30 159 30 1413 30 753
Cuts at: 5722 5752 1836
Size: 30 5242
Fragments arranged by size:
5242 1441 1413 753 159 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 268 1372 1405 1554 1582 1951 2026 2083
Size: 3 1104 33 149 28 369 75 57
Cuts at: 2083 2152 2359 3310 3331 3334 3402 3808 4108
Size: 69 207 951 21 3 68 406 300
Cuts at: 4108 4585 5242 5458 5746 5991 6000 265
Size: 477 657 216 288 245 9 3423
Fragments arranged by size:
3423 1104 951 657 477 406 369 300
288 245 216 207 149 75 69 68
57 33 28 21 9 3 3
BseYI C'CCAG_C
Cuts at: 269 554 1102 1226 2180 2493 3112 3341 4605
Size: 285 548 124 954 313 619 229 1264
Cuts at: 4605 5240 5337 6583 7341 7918 8414 269
Size: 635 97 1246 758 577 496 1013
Fragments arranged by size:
1264 1246 1013 954 758 635 619 577
548 496 313 285 229 124 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 2957 640
Size: 2317 6841
BsiEI CG_ry'CG
Cuts at: 104 5237 6700 6746 7954 104
Size: 5133 1463 46 1208 1308
Fragments arranged by size:
5133 1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 332 526 1985 2082 2321 3554 3716 4026 4044
Size: 194 1459 97 239 1233 162 310 18
Cuts at: 4044 4928 5452 6172 6950 7140 7932 332
Size: 884 524 720 778 190 792 1558
Fragments arranged by size:
1558 1459 1233 884 792 778 720 524
310 239 194 190 162 97 18
BsmBI CGTCTCn'nnnn_
Cuts at: 2179 3145 3951 2179
Size: 966 806 7386
Fragments arranged by size:
7386 966 806
Bsp1286I G_dGCh'C
Cuts at: 136 332 344 526 786 834 1799 1824 1985
Size: 196 12 182 260 48 965 25 161
Cuts at: 1985 2027 2082 2094 2321 2622 3220 3239 3251
Size: 42 55 12 227 301 598 19 12
Cuts at: 3251 3313 3554 3716 3997 4026 4044 4612 4921
Size: 62 241 162 281 29 18 568 309
Cuts at: 4921 4928 5338 5452 5459 5777 6172 6783 6876
Size: 7 410 114 7 318 395 611 93
Cuts at: 6876 6950 7140 7202 7932 136
Size: 74 190 62 730 1362
Fragments arranged by size:
1362 965 730 611 598 568 410 395
318 309 301 281 260 241 227 196
190 182 162 161 114 93 74 62
62 55 48 42 29 25 19 18
12 12 12 7 7
BspEI T'CCGG_A
Cuts at: 467 1113 3372 6126 8378 467
Size: 646 2259 2754 2252 1247
Fragments arranged by size:
2754 2259 2252 1247 646
BspHI T'CATG_A
Cuts at: 3743 4106 4562 5054 8334 3743
Size: 363 456 492 3280 4567
Fragments arranged by size:
4567 3280 492 456 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 140 287 1058 2346 3703 4335 4805 5630 5858
Size: 147 771 1288 1357 632 470 825 228
Cuts at: 5858 6208 6422 6724 7105 140
Size: 350 214 302 381 2193
Fragments arranged by size:
2193 1357 1288 825 771 632 470 381
350 302 228 214 147
BsrBI CCG'CTC
Cuts at: 50 475 3798 7450 7504 8988 9153 50
Size: 425 3323 3652 54 1484 165 55
Fragments arranged by size:
3652 3323 1484 425 165 55 54
BsrDI GCAATG_nn'
Cuts at: 753 1352 1628 1905 2411 3119 5316 5405 5649
Size: 599 276 277 506 708 2197 89 244
Cuts at: 5649 7070 753
Size: 1421 2841
Fragments arranged by size:
2841 2197 1421 708 599 506 277 276
244 89
BsrFI r'CCGG_y
Cuts at: 1124 5236 7156 7337 7546 1124
Size: 4112 1920 181 209 2736
Fragments arranged by size:
4112 2736 1920 209 181
BsrGI T'GTAC_A
Cuts at: 4442 5402 4442
Size: 960 8198
BssSI C'ACGA_G
Cuts at: 1441 7429 7787 1441
Size: 5988 358 2812
Fragments arranged by size:
5988 2812 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 3091 3695 5210 5651 5853 6535 1735
Size: 1356 604 1515 441 202 682 4358
Fragments arranged by size:
4358 1515 1356 682 604 441 202
BstBI TT'CG_AA
Cuts at: 7519 7519
Size: 9158
BstEII G'GTnAC_C
Cuts at: 4731 4731
Size: 9158
BstXI CCAn_nnnn'nTGG
Cuts at: 689 2124 2165 3258 3975 4743 5418 689
Size: 1435 41 1093 717 768 675 4429
Fragments arranged by size:
4429 1435 1093 768 717 675 41
BstYI r'GATC_y
Cuts at: 1 303 1268 3228 3563 5030 5492 5720 5762
Size: 302 965 1960 335 1467 462 228 42
Cuts at: 5762 6102 6183 6650 6673 7008 7254 8266 8352
Size: 340 81 467 23 335 246 1012 86
Cuts at: 8352 8364 8710 1
Size: 12 346 449
Fragments arranged by size:
1960 1467 1012 965 467 462 449 346
340 335 335 302 246 228 86 81
42 23 12
Bsu36I CC'TnA_GG
Cuts at: 934 1252 934
Size: 318 8840
BtgI C'CryG_G
Cuts at: 107 207 682 986 1155 1337 1418 1644 1683
Size: 100 475 304 169 182 81 226 39
Cuts at: 1683 1869 4636 5342 5438 5627 5819 107
Size: 186 2767 706 96 189 192 3446
Fragments arranged by size:
3446 2767 706 475 304 226 192 189
186 182 169 100 96 81 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1362 3237 5931 7108 7289 7452 1362
Size: 1875 2694 1177 181 163 3068
Fragments arranged by size:
3068 2694 1875 1177 181 163
BtsI GCAGTG_nn'
Cuts at: 1786 3463 3765 4006 6401 1786
Size: 1677 302 241 2395 4543
Fragments arranged by size:
4543 2395 1677 302 241
DraI TTT'AAA
Cuts at: 4359 6157 4359
Size: 1798 7360
DraIII CAC_nnn'GTG
Cuts at: 2282 3708 2282
Size: 1426 7732
DrdI GACnn_nn'nnGTC
Cuts at: 1003 1254 1835 6864 7722 1003
Size: 251 581 5029 858 2439
Fragments arranged by size:
5029 2439 858 581 251
EaeI y'GGCC_r
Cuts at: 1334 1857 2561 3260 3576 3965 4784 4808 5132
Size: 523 704 699 316 389 819 24 324
Cuts at: 5132 5234 5435 5816 6743 6917 7308 7335 8680
Size: 102 201 381 927 174 391 27 1345
Cuts at: 8680 1334
Size: 1812
Fragments arranged by size:
1812 1345 927 819 704 699 523 391
389 381 324 316 201 174 102 27
24
EagI C'GGCC_G
Cuts at: 5234 6743 5234
Size: 1509 7649
EarI CTCTTCn'nnn_
Cuts at: 508 925 1207 2123 2308 2911 3281 4403 4972
Size: 417 282 916 185 603 370 1122 569
Cuts at: 4972 5080 5101 5221 5359 5801 5890 5912 7181
Size: 108 21 120 138 442 89 22 1269
Cuts at: 7181 7391 508
Size: 210 2275
Fragments arranged by size:
2275 1269 1122 916 603 569 442 417
370 282 210 185 138 120 108 89
22 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1195 1954 2527 4969 7366 7676 7822 8968 1195
Size: 759 573 2442 2397 310 146 1146 1385
Fragments arranged by size:
2442 2397 1385 1146 759 573 310 146
EcoICRI GAG'CTC
Cuts at: 330 3552 4042 6170 330
Size: 3222 490 2128 3318
Fragments arranged by size:
3318 3222 2128 490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 835 1541 1745 2335 2916 2968 3408 3577
Size: 468 706 204 590 581 52 440 169
Cuts at: 3577 3632 3880 4131 4380 5412 5440 5737 5979
Size: 55 248 251 249 1032 28 297 242
Cuts at: 5979 6052 6983 7415 8162 8430 367
Size: 73 931 432 747 268 1095
Fragments arranged by size:
1095 1032 931 747 706 590 581 468
440 432 297 268 251 249 248 242
204 169 73 55 52 28
EcoNI CCTnn'n_nnAGG
Cuts at: 1704 3577 4342 5464 5841 1704
Size: 1873 765 1122 377 5021
Fragments arranged by size:
5021 1873 1122 765 377
EcoO109I rG'GnC_Cy
Cuts at: 351 678 783 830 831 1307 1446 2024 2091
Size: 327 105 47 1 476 139 578 67
Cuts at: 2091 3012 3366 3383 3477 3591 3660 3994 5685
Size: 921 354 17 94 114 69 334 1691
Cuts at: 5685 5940 6297 351
Size: 255 357 3212
Fragments arranged by size:
3212 1691 921 578 476 357 354 334
327 255 139 114 105 94 69 67
47 17 1
EcoRI G'AATT_C
Cuts at: 2338 6162 2338
Size: 3824 5334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 429 3918 4707 4968 7177 429
Size: 3489 789 261 2209 2410
Fragments arranged by size:
3489 2410 2209 789 261
FspI TGC'GCA
Cuts at: 952 3688 6939 952
Size: 2736 3251 3171
Fragments arranged by size:
3251 3171 2736
HaeII r_GCGC'y
Cuts at: 967 1236 1407 2187 2314 3630 3769 4094 6592
Size: 269 171 780 127 1316 139 325 2498
Cuts at: 6592 6840 7862 8841 967
Size: 248 1022 979 1284
Fragments arranged by size:
2498 1316 1284 1022 979 780 325 269
248 171 139 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 624 656 1459 1491 1927 1959 2707 2739 2789
Size: 32 803 32 436 32 748 32 50
Cuts at: 2789 2821 3307 3339 3496 3528 5551 5583 6127
Size: 32 486 32 157 32 2023 32 544
Cuts at: 6127 6159 7000 7030 7032 7062 624
Size: 32 841 30 2 30 2720
Fragments arranged by size:
2720 2023 841 803 748 544 486 436
157 50 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4305 5397 6197 4305
Size: 1092 800 7266
Fragments arranged by size:
7266 1092 800
KasI G'GCGC_C
Cuts at: 4090 6588 6836 4090
Size: 2498 248 6412
Fragments arranged by size:
6412 2498 248
KpnI G_GTAC'C
Cuts at: 4332 6178 4332
Size: 1846 7312
MluI A'CGCG_T
Cuts at: 659 8591 659
Size: 7932 1226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2340 2463 7829 8013 2340
Size: 123 5366 184 3485
Fragments arranged by size:
5366 3485 184 123
MscI TGG'CCA
Cuts at: 1859 2563 3967 4786 4810 5134 5437 6919 1859
Size: 704 1404 819 24 324 303 1482 4098
Fragments arranged by size:
4098 1482 1404 819 704 324 303 24
MslI CAynn'nnrTG
Cuts at: 1423 2278 2653 2830 3256 3742 4066 4159 4234
Size: 855 375 177 426 486 324 93 75
Cuts at: 4234 4561 4705 4741 5614 7147 7274 1423
Size: 327 144 36 873 1533 127 3307
Fragments arranged by size:
3307 1533 873 855 486 426 375 327
324 177 144 127 93 75 36
MspA1I CmG'CkG
Cuts at: 209 273 1685 1759 2088 2211 2988 3069 3108
Size: 64 1412 74 329 123 777 81 39
Cuts at: 3108 3116 3547 3719 4387 4874 5906 6269 6583
Size: 8 431 172 668 487 1032 363 314
Cuts at: 6583 6943 7956 8201 209
Size: 360 1013 245 1166
Fragments arranged by size:
1412 1166 1032 1013 777 668 487 431
363 360 329 314 245 172 123 81
74 64 39 8
NaeI GCC'GGC
Cuts at: 5238 7339 5238
Size: 2101 7057
NarI GG'CG_CC
Cuts at: 4091 6589 6837 4091
Size: 2498 248 6412
Fragments arranged by size:
6412 2498 248
NcoI C'CATG_G
Cuts at: 107 682 1418 1869 5627 5819 107
Size: 575 736 451 3758 192 3446
Fragments arranged by size:
3758 3446 736 575 451 192
NdeI CA'TA_TG
Cuts at: 3846 3846
Size: 9158
NgoMIV G'CCGG_C
Cuts at: 5236 7337 5236
Size: 2101 7057
NheI G'CTAG_C
Cuts at: 2643 2643
Size: 9158
NspI r_CATG'y
Cuts at: 513 1112 2682 4443 5178 5208 5403 5875 6211
Size: 599 1570 1761 735 30 195 472 336
Cuts at: 6211 6711 7618 513
Size: 500 907 2053
Fragments arranged by size:
2053 1761 1570 907 735 599 500 472
336 195 30
PasI CC'CwG_GG
Cuts at: 1824 3099 3481 3664 3975 1824
Size: 1275 382 183 311 7007
Fragments arranged by size:
7007 1275 382 311 183
PciI A'CATG_T
Cuts at: 1108 4439 5174 5399 7614 1108
Size: 3331 735 225 2215 2652
Fragments arranged by size:
3331 2652 2215 735 225
PflMI CCAn_nnn'nTGG
Cuts at: 2570 3386 3708 3914 4874 5655 2570
Size: 816 322 206 960 781 6073
Fragments arranged by size:
6073 960 816 781 322 206
PfoI T'CCnGG_A
Cuts at: 1409 1839 3379 3554 5417 1409
Size: 430 1540 175 1863 5150
Fragments arranged by size:
5150 1863 1540 430 175
PmeI GTTT'AAAC
Cuts at: 6157 6157
Size: 9158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1051 1083 1836 1868 5722 5754 8297 8329 1051
Size: 32 753 32 3854 32 2543 32 1880
Fragments arranged by size:
3854 2543 1880 753 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 351 678 1307 3383 3591 5685 351
Size: 327 629 2076 208 2094 3824
Fragments arranged by size:
3824 2094 2076 629 327 208
PshAI GACnn'nnGTC
Cuts at: 4760 4760
Size: 9158
PspOMI G'GGCC_C
Cuts at: 782 830 2023 2090 3993 782
Size: 48 1193 67 1903 5947
Fragments arranged by size:
5947 1903 1193 67 48
PspXI vC'TCGA_Gb
Cuts at: 641 641
Size: 9158
PstI C_TGCA'G
Cuts at: 284 917 2357 4346 6205 6890 284
Size: 633 1440 1989 1859 685 2552
Fragments arranged by size:
2552 1989 1859 1440 685 633
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9158
PvuII CAG'CTG
Cuts at: 273 2988 3108 3116 3719 4387 4874 6583 6943
Size: 2715 120 8 603 668 487 1709 360
Cuts at: 6943 273
Size: 2488
Fragments arranged by size:
2715 2488 1709 668 603 487 360 120
8
RsrII CG'GwC_CG
Cuts at: 7353 7353
Size: 9158
SacI G_AGCT'C
Cuts at: 332 3554 4044 6172 332
Size: 3222 490 2128 3318
Fragments arranged by size:
3318 3222 2128 490
SacII CC_GC'GG
Cuts at: 210 1686 210
Size: 1476 7682
SalI G'TCGA_C
Cuts at: 6195 6195
Size: 9158
SanDI GG'GwC_CC
Cuts at: 678 678
Size: 9158
SapI GCTCTTCn'nnn_
Cuts at: 508 925 4403 4972 5101 7181 7391 508
Size: 417 3478 569 129 2080 210 2275
Fragments arranged by size:
3478 2275 2080 569 417 210 129
SbfI CC_TGCA'GG
Cuts at: 284 6205 284
Size: 5921 3237
ScaI AGT'ACT
Cuts at: 3901 4585 4948 8675 3901
Size: 684 363 3727 4384
Fragments arranged by size:
4384 3727 684 363
SexAI A'CCwGG_T
Cuts at: 1316 2366 1316
Size: 1050 8108
SfcI C'TryA_G
Cuts at: 33 280 366 913 2353 4342 6087 6201 6886
Size: 247 86 547 1440 1989 1745 114 685
Cuts at: 6886 7879 8070 33
Size: 993 191 1121
Fragments arranged by size:
1989 1745 1440 1121 993 685 547 247
191 114 86
SfoI GGC'GCC
Cuts at: 4092 6590 6838 4092
Size: 2498 248 6412
Fragments arranged by size:
6412 2498 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9158
SmaI CCC'GGG
Cuts at: 3225 6180 3225
Size: 2955 6203
SmlI C'TyrA_G
Cuts at: 641 1054 1429 3820 3871 6313 7228 7720 7982
Size: 413 375 2391 51 2442 915 492 262
Cuts at: 7982 641
Size: 1817
Fragments arranged by size:
2442 2391 1817 915 492 413 375 262
51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9158
SphI G_CATG'C
Cuts at: 2682 5208 5875 6211 6711 2682
Size: 2526 667 336 500 5129
Fragments arranged by size:
5129 2526 667 500 336
StuI AGG'CCT
Cuts at: 286 2729 4690 286
Size: 2443 1961 4754
Fragments arranged by size:
4754 2443 1961
StyI C'CwwG_G
Cuts at: 107 682 1418 1487 1869 4292 5024 5627 5819
Size: 575 736 69 382 2423 732 603 192
Cuts at: 5819 5982 6292 6646 107
Size: 163 310 354 2619
Fragments arranged by size:
2619 2423 736 732 603 575 382 354
310 192 163 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 388 1402 3867 5529 6741 6874 7333 7542 388
Size: 1014 2465 1662 1212 133 459 209 2004
Fragments arranged by size:
2465 2004 1662 1212 1014 459 209 133
TatI w'GTAC_w
Cuts at: 590 3899 4283 4442 4583 4946 5402 8673 590
Size: 3309 384 159 141 363 456 3271 1075
Fragments arranged by size:
3309 3271 1075 456 384 363 159 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4005 4037 8675 8707 4005
Size: 32 4638 32 4456
Fragments arranged by size:
4638 4456 32 32
Tth111I GACn'n_nGTC
Cuts at: 6040 6955 6040
Size: 915 8243
XbaI T'CTAG_A
Cuts at: 6189 6189
Size: 9158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 984 1461 1867 3671 4602 5127 5685 6653 984
Size: 477 406 1804 931 525 558 968 3489
Fragments arranged by size:
3489 1804 968 931 558 525 477 406
XhoI C'TCGA_G
Cuts at: 641 641
Size: 9158
XmaI C'CCGG_G
Cuts at: 3223 6178 3223
Size: 2955 6203
XmnI GAAnn'nnTTC
Cuts at: 2455 2905 3901 4882 4948 2455
Size: 450 996 981 66 6665
Fragments arranged by size:
6665 996 981 450 66
ZraI GAC'GTC
Cuts at: 3945 4698 3945
Size: 753 8405
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BsiWI BsmI BssHII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NotI NruI NsiI PacI PmlI PsiI PsrI
SfiI SgrAI SnaBI SrfI SspI SwaI