Restriction map for
pF1KE2369
(Circular) (Six-base) MAPSORT of: pF1KE2369.seq Check: 9951 from: 1 to: 4853
pF1KE2369 4853 bp
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AccI GT'mk_AC
Cuts at: 1891 1891
Size: 4853
Acc65I G'GTAC_C
Cuts at: 1112 1203 1869 1112
Size: 91 666 4096
Fragments arranged by size:
4096 666 91
AclI AA'CG_TT
Cuts at: 4693 4693
Size: 4853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 3110 3857 4125 2678
Size: 432 747 268 3406
Fragments arranged by size:
3406 747 432 268
AflII C'TTAA_G
Cuts at: 900 900
Size: 4853
AflIII A'CryG_T
Cuts at: 1003 1323 2205 3309 4286 1003
Size: 320 882 1104 977 1570
Fragments arranged by size:
1570 1104 977 882 320
AgeI A'CCGG_T
Cuts at: 3241 3241
Size: 4853
AhdI GACnn_n'nnGTC
Cuts at: 597 597
Size: 4853
AleI CACnn'nnGTG
Cuts at: 1546 1546
Size: 4853
AlwNI CAG_nnn'CTG
Cuts at: 207 265 1045 2158 3725 207
Size: 58 780 1113 1567 1335
Fragments arranged by size:
1567 1335 1113 780 58
ApaLI G'TGCA_C
Cuts at: 1327 3623 1327
Size: 2296 2557
ApoI r'AATT_y
Cuts at: 1471 1857 4206 4462 1471
Size: 386 2349 256 1862
Fragments arranged by size:
2349 1862 386 256
AscI GG'CGCG_CC
Cuts at: 483 483
Size: 4853
AseI AT'TA_AT
Cuts at: 20 20
Size: 4853
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
AvaI C'yCGr_G
Cuts at: 301 322 1873 301
Size: 21 1551 3281
Fragments arranged by size:
3281 1551 21
BamHI G'GATC_C
Cuts at: 1878 1878
Size: 4853
BanI G'GyrC_C
Cuts at: 427 583 1112 1203 1869 2283 2531 2566 427
Size: 156 529 91 666 414 248 35 2714
Fragments arranged by size:
2714 666 529 414 248 156 91 35
BanII G_rGCy'C
Cuts at: 1691 1867 2897 1691
Size: 176 1030 3647
Fragments arranged by size:
3647 1030 176
BbeI G_GCGC'C
Cuts at: 587 2287 2535 587
Size: 1700 248 2905
Fragments arranged by size:
2905 1700 248
BbsI GAAGACnn'nnnn_
Cuts at: 1024 1619 1024
Size: 595 4258
BbvCI CC'TCA_GC
Cuts at: 294 294
Size: 4853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 667 633
Size: 34 4819
BciVI GTATCCnnnnn_n'
Cuts at: 1338 2751 3512 4853 1338
Size: 1413 761 1341 1338
Fragments arranged by size:
1413 1341 1338 761
BclI T'GATC_A
Cuts at: 662 1119 2373 662
Size: 457 1254 3142
Fragments arranged by size:
3142 1254 457
BglII A'GATC_T
Cuts at: 2368 4405 2368
Size: 2037 2816
BlpI GC'TnA_GC
Cuts at: 148 511 1775 1965 148
Size: 363 1264 190 3036
Fragments arranged by size:
3036 1264 363 190
Bme1580I G_kGCm'C
Cuts at: 413 1331 2478 2571 3627 413
Size: 918 1147 93 1056 1639
Fragments arranged by size:
1639 1147 1056 918 93
BmgBI CAC'GTC
Cuts at: 1761 1761
Size: 4853
BmrI ACTGGGnnnn_n'
Cuts at: 2065 2146 2481 4625 2065
Size: 81 335 2144 2293
Fragments arranged by size:
2293 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 3220 207
Size: 3013 1840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 744 401
Size: 343 4510
Bpu10I CC'TnA_GC
Cuts at: 294 612 294
Size: 318 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 2029 2908 3400 3698 177
Size: 1852 879 492 298 1332
Fragments arranged by size:
1852 1332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 4557 559
Size: 3998 855
BsaAI yAC'GTr
Cuts at: 1326 2206 1326
Size: 880 3973
BsaBI GATnn'nnATC
Cuts at: 6 2367 4404 6
Size: 2361 2037 455
Fragments arranged by size:
2361 2037 455
BsaHI Gr'CG_yC
Cuts at: 134 584 598 2284 2532 3234 4730 134
Size: 450 14 1686 248 702 1496 257
Fragments arranged by size:
1686 1496 702 450 257 248 14
BsaWI w'CCGG_w
Cuts at: 2042 2563 3241 3515 3662 4073 2042
Size: 521 678 274 147 411 2822
Fragments arranged by size:
2822 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 319 349 319
Size: 30 4823
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 398 951 245
Size: 153 553 4147
Fragments arranged by size:
4147 553 153
BseYI C'CCAG_C
Cuts at: 471 2278 3036 3613 4109 471
Size: 1807 758 577 496 1215
Fragments arranged by size:
1807 1215 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 145 2395 2441 3649 104
Size: 41 2250 46 1208 1308
Fragments arranged by size:
2250 1308 1208 46 41
BsiHKAI G_wGCw'C
Cuts at: 329 981 1331 1867 2645 2835 3627 329
Size: 652 350 536 778 190 792 1555
Fragments arranged by size:
1555 792 778 652 536 350 190
Bsp1286I G_dGCh'C
Cuts at: 329 413 981 1331 1691 1867 2478 2571 2645
Size: 84 568 350 360 176 611 93 74
Cuts at: 2645 2835 2897 3627 329
Size: 190 62 730 1555
Fragments arranged by size:
1555 730 611 568 360 350 190 176
93 84 74 62
BspEI T'CCGG_A
Cuts at: 4073 4073
Size: 4853
BspHI T'CATG_A
Cuts at: 1337 4029 1337
Size: 2692 2161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 110 1903 2117 2419 2800 110
Size: 1793 214 302 381 2163
Fragments arranged by size:
2163 1793 381 302 214
BsrBI CCG'CTC
Cuts at: 50 147 407 1779 3145 3199 4683 4848 50
Size: 97 260 1372 1366 54 1484 165 55
Fragments arranged by size:
1484 1372 1366 260 165 97 55 54
BsrDI GCAATG_nn'
Cuts at: 2765 2765
Size: 4853
BsrFI r'CCGG_y
Cuts at: 361 479 2851 3032 3241 361
Size: 118 2372 181 209 1973
Fragments arranged by size:
2372 1973 209 181 118
BssHII G'CGCG_C
Cuts at: 483 483
Size: 4853
BssSI C'ACGA_G
Cuts at: 181 3124 3482 181
Size: 2943 358 1552
Fragments arranged by size:
2943 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 207 265 2230 207
Size: 58 1965 2830
Fragments arranged by size:
2830 1965 58
BstBI TT'CG_AA
Cuts at: 494 3214 494
Size: 2720 2133
BstXI CCAn_nnnn'nTGG
Cuts at: 345 345
Size: 4853
BstYI r'GATC_y
Cuts at: 1 1878 2345 2368 2703 2949 3961 4047 4059
Size: 1877 467 23 335 246 1012 86 12
Cuts at: 4059 4405 1
Size: 346 449
Fragments arranged by size:
1877 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1168 2803 2984 3147 1168
Size: 1635 181 163 2874
Fragments arranged by size:
2874 1635 181 163
BtsI GCAGTG_nn'
Cuts at: 429 2096 429
Size: 1667 3186
DraI TTT'AAA
Cuts at: 1852 1852
Size: 4853
DrdI GACnn_nn'nnGTC
Cuts at: 573 1759 2559 3417 573
Size: 1186 800 858 2009
Fragments arranged by size:
2009 1186 858 800
EaeI y'GGCC_r
Cuts at: 142 2438 2612 3003 3030 4375 142
Size: 2296 174 391 27 1345 620
Fragments arranged by size:
2296 1345 620 391 174 27
EagI C'GGCC_G
Cuts at: 142 2438 142
Size: 2296 2557
EarI CTCTTCn'nnn_
Cuts at: 1618 1818 2876 3086 1618
Size: 200 1058 210 3385
Fragments arranged by size:
3385 1058 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 383 3061 3371 3517 4663 383
Size: 2678 310 146 1146 573
Fragments arranged by size:
2678 1146 573 310 146
EcoICRI GAG'CTC
Cuts at: 1865 1865
Size: 4853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 744 2678 3110 3857 4125 401
Size: 343 1934 432 747 268 1129
Fragments arranged by size:
1934 1129 747 432 343 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1794 1794
Size: 4853
EcoO109I rG'GnC_Cy
Cuts at: 1992 1992
Size: 4853
EcoRI G'AATT_C
Cuts at: 1857 1857
Size: 4853
EcoRV GAT'ATC
Cuts at: 1166 1166
Size: 4853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1011 2872 1011
Size: 1861 2992
FspI TGC'GCA
Cuts at: 2634 2634
Size: 4853
HaeII r_GCGC'y
Cuts at: 352 587 2287 2535 3557 4536 352
Size: 235 1700 248 1022 979 669
Fragments arranged by size:
1700 1022 979 669 248 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 583 615 946 978 1332 1364 2695 2725 2727
Size: 32 331 32 354 32 1331 30 2
Cuts at: 2727 2757 583
Size: 30 2679
Fragments arranged by size:
2679 1331 354 331 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1892 1892
Size: 4853
KasI G'GCGC_C
Cuts at: 583 2283 2531 583
Size: 1700 248 2905
Fragments arranged by size:
2905 1700 248
KpnI G_GTAC'C
Cuts at: 1116 1207 1873 1116
Size: 91 666 4096
Fragments arranged by size:
4096 666 91
MluI A'CGCG_T
Cuts at: 4286 4286
Size: 4853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 362 3524 3708 207
Size: 155 3162 184 1352
Fragments arranged by size:
3162 1352 184 155
MscI TGG'CCA
Cuts at: 2614 2614
Size: 4853
MslI CAynn'nnrTG
Cuts at: 816 1546 2842 2969 816
Size: 730 1296 127 2700
Fragments arranged by size:
2700 1296 730 127
MspA1I CmG'CkG
Cuts at: 116 265 283 865 1353 1378 1964 2278 2638
Size: 149 18 582 488 25 586 314 360
Cuts at: 2638 3651 3896 116
Size: 1013 245 1073
Fragments arranged by size:
1073 1013 586 582 488 360 314 245
149 25 18
NaeI GCC'GGC
Cuts at: 3034 3034
Size: 4853
NarI GG'CG_CC
Cuts at: 584 2284 2532 584
Size: 1700 248 2905
Fragments arranged by size:
2905 1700 248
NgoMIV G'CCGG_C
Cuts at: 3032 3032
Size: 4853
NotI GC'GGCC_GC
Cuts at: 142 142
Size: 4853
NspI r_CATG'y
Cuts at: 540 1906 2406 3313 540
Size: 1366 500 907 2080
Fragments arranged by size:
2080 1366 907 500
PasI CC'CwG_GG
Cuts at: 1684 1684
Size: 4853
PciI A'CATG_T
Cuts at: 3309 3309
Size: 4853
PflMI CCAn_nnn'nTGG
Cuts at: 186 186
Size: 4853
PfoI T'CCnGG_A
Cuts at: 1795 1795
Size: 4853
PmeI GTTT'AAAC
Cuts at: 1852 1852
Size: 4853
PmlI CAC'GTG
Cuts at: 1326 1326
Size: 4853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4821
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 448 480 448
Size: 32 4821
PstI C_TGCA'G
Cuts at: 121 212 261 1900 2585 121
Size: 91 49 1639 685 2389
Fragments arranged by size:
2389 1639 685 91 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4853
PvuII CAG'CTG
Cuts at: 116 1353 1378 2278 2638 116
Size: 1237 25 900 360 2331
Fragments arranged by size:
2331 1237 900 360 25
RsrII CG'GwC_CG
Cuts at: 3048 3048
Size: 4853
SacI G_AGCT'C
Cuts at: 1867 1867
Size: 4853
SalI G'TCGA_C
Cuts at: 1890 1890
Size: 4853
SapI GCTCTTCn'nnn_
Cuts at: 2876 3086 2876
Size: 210 4643
SbfI CC_TGCA'GG
Cuts at: 1900 1900
Size: 4853
ScaI AGT'ACT
Cuts at: 4370 4370
Size: 4853
SfcI C'TryA_G
Cuts at: 33 117 208 257 1283 1896 2581 3574 3765
Size: 84 91 49 1026 613 685 993 191
Cuts at: 3765 33
Size: 1121
Fragments arranged by size:
1121 1026 993 685 613 191 91 84
49
SfoI GGC'GCC
Cuts at: 585 2285 2533 585
Size: 1700 248 2905
Fragments arranged by size:
2905 1700 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4853
SgrAI Cr'CCGG_yG
Cuts at: 479 479
Size: 4853
SmaI CCC'GGG
Cuts at: 1875 1875
Size: 4853
SmlI C'TyrA_G
Cuts at: 156 900 2008 2923 3415 3677 156
Size: 744 1108 915 492 262 1332
Fragments arranged by size:
1332 1108 915 744 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4853
SphI G_CATG'C
Cuts at: 1906 2406 1906
Size: 500 4353
StyI C'CwwG_G
Cuts at: 1146 1987 2341 1146
Size: 841 354 3658
Fragments arranged by size:
3658 841 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 583 646 1243 2436 2569 3028 3237 583
Size: 63 597 1193 133 459 209 2199
Fragments arranged by size:
2199 1193 597 459 209 133 63
TatI w'GTAC_w
Cuts at: 1047 1386 4368 1047
Size: 339 2982 1532
Fragments arranged by size:
2982 1532 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 319 351 4370 4402 319
Size: 32 4019 32 770
Fragments arranged by size:
4019 770 32 32
Tth111I GACn'n_nGTC
Cuts at: 2650 2650
Size: 4853
XbaI T'CTAG_A
Cuts at: 964 1884 964
Size: 920 3933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 1681 2348 867
Size: 814 667 3372
Fragments arranged by size:
3372 814 667
XmaI C'CCGG_G
Cuts at: 1873 1873
Size: 4853
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AvrII BaeI
BfrBI BglI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI