Restriction map for
pF1KE2379
(Circular) (Six-base) MAPSORT of: pF1KE2379.seq Check: 1051 from: 1 to: 6590
pF1KE2379 6590 bp
With 182 enzymes: SgfI *
October 18, 2011 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 310 310
Size: 6590
AatII G_ACGT'C
Cuts at: 2489 2489
Size: 6590
AccI GT'mk_AC
Cuts at: 3628 3628
Size: 6590
Acc65I G'GTAC_C
Cuts at: 3606 3606
Size: 6590
AclI AA'CG_TT
Cuts at: 6430 6430
Size: 6590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1426 1699 1947 2278 2733 4415 4847 5594
Size: 804 273 248 331 455 1682 432 747
Cuts at: 5594 5862 622
Size: 268 1350
Fragments arranged by size:
1682 1350 804 747 455 432 331 273
268 248
AflIII A'CryG_T
Cuts at: 2081 2636 3942 5046 6023 2081
Size: 555 1306 1104 977 2648
Fragments arranged by size:
2648 1306 1104 977 555
AgeI A'CCGG_T
Cuts at: 4978 4978
Size: 6590
AleI CACnn'nnGTG
Cuts at: 1978 2413 1978
Size: 435 6155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1066 1066
Size: 6590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1477 1509 1477
Size: 32 6558
AlwNI CAG_nnn'CTG
Cuts at: 363 1239 1461 2103 2139 3107 3555 3895 5462
Size: 876 222 642 36 968 448 340 1567
Cuts at: 5462 363
Size: 1491
Fragments arranged by size:
1567 1491 968 876 642 448 340 222
36
ApaI G_GGCC'C
Cuts at: 556 788 2367 2580 2944 3480 3540 556
Size: 232 1579 213 364 536 60 3606
Fragments arranged by size:
3606 1579 536 364 232 213 60
ApaLI G'TGCA_C
Cuts at: 1770 5360 1770
Size: 3590 3000
ApoI r'AATT_y
Cuts at: 3594 5943 6199 3594
Size: 2349 256 3985
Fragments arranged by size:
3985 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
AvaI C'yCGr_G
Cuts at: 209 632 678 781 860 888 923 2109 2197
Size: 423 46 103 79 28 35 1186 88
Cuts at: 2197 2456 2712 3195 3209 3357 3471 3576 3610
Size: 259 256 483 14 148 114 105 34
Cuts at: 3610 209
Size: 3189
Fragments arranged by size:
3189 1186 483 423 259 256 148 114
105 103 88 79 46 35 34 28
14
BamHI G'GATC_C
Cuts at: 3615 3615
Size: 6590
BanI G'GyrC_C
Cuts at: 370 444 673 717 1249 1281 2973 3073 3606
Size: 74 229 44 532 32 1692 100 533
Cuts at: 3606 4020 4268 4303 370
Size: 414 248 35 2657
Fragments arranged by size:
2657 1692 533 532 414 248 229 100
74 44 35 32
BanII G_rGCy'C
Cuts at: 556 782 788 895 2367 2464 2580 2719 2899
Size: 226 6 107 1472 97 116 139 180
Cuts at: 2899 2944 3023 3143 3480 3540 3604 4634 556
Size: 45 79 120 337 60 64 1030 2512
Fragments arranged by size:
2512 1472 1030 337 226 180 139 120
116 107 97 79 64 60 45 6
BbeI G_GCGC'C
Cuts at: 374 677 1253 3077 4024 4272 374
Size: 303 576 1824 947 248 2692
Fragments arranged by size:
2692 1824 947 576 303 248
BbsI GAAGACnn'nnnn_
Cuts at: 1368 1687 1368
Size: 319 6271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 914 948 90
Size: 34 790 34 5732
Fragments arranged by size:
5732 790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4488 5249 6590 4488
Size: 761 1341 4488
Fragments arranged by size:
4488 1341 761
BclI T'GATC_A
Cuts at: 4110 4110
Size: 6590
BglI GCCn_nnn'nGGC
Cuts at: 682 958 1565 1916 2880 682
Size: 276 607 351 964 4392
Fragments arranged by size:
4392 964 607 351 276
BglII A'GATC_T
Cuts at: 2021 4105 6142 2021
Size: 2084 2037 2469
Fragments arranged by size:
2469 2084 2037
BlpI GC'TnA_GC
Cuts at: 3702 3702
Size: 6590
Bme1580I G_kGCm'C
Cuts at: 449 556 788 1774 2116 2367 2580 2944 2978
Size: 107 232 986 342 251 213 364 34
Cuts at: 2978 3480 3540 4215 4308 5364 449
Size: 502 60 675 93 1056 1675
Fragments arranged by size:
1675 1056 986 675 502 364 342 251
232 213 107 93 60 34
BmgBI CAC'GTC
Cuts at: 129 699 1257 3219 129
Size: 570 558 1962 3500
Fragments arranged by size:
3500 1962 570 558
BmrI ACTGGGnnnn_n'
Cuts at: 3438 3802 3883 4218 6362 3438
Size: 364 81 335 2144 3666
Fragments arranged by size:
3666 2144 364 335 81
BmtI G_CTAG'C
Cuts at: 1801 2069 1801
Size: 268 6322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4957 4957
Size: 6590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 2644 2695 2898 2940 2967 3174 3226 3283
Size: 1765 51 203 42 27 207 52 57
Cuts at: 3283 879
Size: 4186
Fragments arranged by size:
4186 1765 207 203 57 52 51 42
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 3766 4645 5137 5435 1120
Size: 2646 879 492 298 2275
Fragments arranged by size:
2646 2275 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3259 6294 3259
Size: 3035 3555
BsaAI yAC'GTr
Cuts at: 771 3943 771
Size: 3172 3418
BsaBI GATnn'nnATC
Cuts at: 6 4104 6141 6
Size: 4098 2037 455
Fragments arranged by size:
4098 2037 455
BsaHI Gr'CG_yC
Cuts at: 371 674 709 884 1250 2486 3074 3510 4021
Size: 303 35 175 366 1236 588 436 511
Cuts at: 4021 4269 4971 6467 371
Size: 248 702 1496 494
Fragments arranged by size:
1496 1236 702 588 511 494 436 366
303 248 175 35
BsaWI w'CCGG_w
Cuts at: 404 3779 4300 4978 5252 5399 5810 404
Size: 3375 521 678 274 147 411 1184
Fragments arranged by size:
3375 1184 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2437 2467 2884 2914 2437
Size: 30 417 30 6113
Fragments arranged by size:
6113 417 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 808 1561 1675 2215 3163 3475 391
Size: 417 753 114 540 948 312 3506
Fragments arranged by size:
3506 948 753 540 417 312 114
BseYI C'CCAG_C
Cuts at: 366 721 2422 3166 3531 3548 4015 4773 5350
Size: 355 1701 744 365 17 467 758 577
Cuts at: 5350 5846 366
Size: 496 1110
Fragments arranged by size:
1701 1110 758 744 577 496 467 365
355 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 298 1849 2281 3299 3541 282
Size: 16 1551 432 1018 242 3331
Fragments arranged by size:
3331 1551 1018 432 242 16
BsiEI CG_ry'CG
Cuts at: 104 652 962 1569 1751 2875 4132 4178 5386
Size: 548 310 607 182 1124 1257 46 1208
Cuts at: 5386 104
Size: 1308
Fragments arranged by size:
1308 1257 1208 1124 607 548 310 182
46
BsiHKAI G_wGCw'C
Cuts at: 1774 3143 3604 4382 4572 5364 1774
Size: 1369 461 778 190 792 3000
Fragments arranged by size:
3000 1369 792 778 461 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1405 2001 1405
Size: 596 5994
Bsp1286I G_dGCh'C
Cuts at: 449 556 782 788 895 1774 2116 2367 2464
Size: 107 226 6 107 879 342 251 97
Cuts at: 2464 2580 2719 2899 2944 2978 3023 3143 3480
Size: 116 139 180 45 34 45 120 337
Cuts at: 3480 3540 3604 4215 4308 4382 4572 4634 5364
Size: 60 64 611 93 74 190 62 730
Cuts at: 5364 449
Size: 1675
Fragments arranged by size:
1675 879 730 611 342 337 251 226
190 180 139 120 116 107 107 97
93 74 64 62 60 45 45 34
6
BspEI T'CCGG_A
Cuts at: 5810 5810
Size: 6590
BspHI T'CATG_A
Cuts at: 534 5766 534
Size: 5232 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 1643 2438 2627 2699 3640 3854 4156 4537
Size: 1333 795 189 72 941 214 302 381
Cuts at: 4537 310
Size: 2363
Fragments arranged by size:
2363 1333 941 795 381 302 214 189
72
BsrBI CCG'CTC
Cuts at: 50 798 2309 4882 4936 6420 6585 50
Size: 748 1511 2573 54 1484 165 55
Fragments arranged by size:
2573 1511 1484 748 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4502 4502
Size: 6590
BsrFI r'CCGG_y
Cuts at: 111 2870 4588 4769 4978 111
Size: 2759 1718 181 209 1723
Fragments arranged by size:
2759 1723 1718 209 181
BssHII G'CGCG_C
Cuts at: 836 981 836
Size: 145 6445
BssSI C'ACGA_G
Cuts at: 1078 4861 5219 1078
Size: 3783 358 2449
Fragments arranged by size:
3783 2449 358
BstAPI GCAn_nnn'nTGC
Cuts at: 363 1038 3967 363
Size: 675 2929 2986
Fragments arranged by size:
2986 2929 675
BstBI TT'CG_AA
Cuts at: 4951 4951
Size: 6590
BstEII G'GTnAC_C
Cuts at: 1227 1227
Size: 6590
BstXI CCAn_nnnn'nTGG
Cuts at: 570 570
Size: 6590
BstYI r'GATC_y
Cuts at: 1 2021 2171 2471 2603 3615 4082 4105 4440
Size: 2020 150 300 132 1012 467 23 335
Cuts at: 4440 4686 5698 5784 5796 6142 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2020 1012 1012 467 449 346 335 300
246 150 132 86 23 12
BtgI C'CryG_G
Cuts at: 686 713 758 788 2884 686
Size: 27 45 30 2096 4392
Fragments arranged by size:
4392 2096 45 30 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 183 839 1557 1770 4540 4721 4884 183
Size: 656 718 213 2770 181 163 1889
Fragments arranged by size:
2770 1889 718 656 213 181 163
BtsI GCAGTG_nn'
Cuts at: 361 1036 2311 2322 2934 3588 3833 361
Size: 675 1275 11 612 654 245 3118
Fragments arranged by size:
3118 1275 675 654 612 245 11
DraI TTT'AAA
Cuts at: 3589 3589
Size: 6590
DrdI GACnn_nn'nnGTC
Cuts at: 4296 5154 4296
Size: 858 5732
EaeI y'GGCC_r
Cuts at: 843 959 1566 1616 1748 2087 2369 2495 2872
Size: 116 607 50 132 339 282 126 377
Cuts at: 2872 3287 4175 4349 4740 4767 6112 843
Size: 415 888 174 391 27 1345 1321
Fragments arranged by size:
1345 1321 888 607 415 391 377 339
282 174 132 126 116 50 27
EagI C'GGCC_G
Cuts at: 959 1566 1748 2872 4175 959
Size: 607 182 1124 1303 3374
Fragments arranged by size:
3374 1303 1124 607 182
EarI CTCTTCn'nnn_
Cuts at: 3037 4613 4823 3037
Size: 1576 210 4804
Fragments arranged by size:
4804 1576 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 586 815 2956 4798 5108 5254 6400 586
Size: 229 2141 1842 310 146 1146 776
Fragments arranged by size:
2141 1842 1146 776 310 229 146
EcoICRI GAG'CTC
Cuts at: 3141 3602 3141
Size: 461 6129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 879 1426 1699 1947 2278 2644 2695 2733
Size: 257 547 273 248 331 366 51 38
Cuts at: 2733 2898 2940 2967 3174 3226 3283 4415 4847
Size: 165 42 27 207 52 57 1132 432
Cuts at: 4847 5594 5862 622
Size: 747 268 1350
Fragments arranged by size:
1350 1132 747 547 432 366 331 273
268 257 248 207 165 57 52 51
42 38 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1405 2623 2781 3028 1405
Size: 1218 158 247 4967
Fragments arranged by size:
4967 1218 247 158
EcoO109I rG'GnC_Cy
Cuts at: 524 552 553 814 1236 2364 2427 2577 2940
Size: 28 1 261 422 1128 63 150 363
Cuts at: 2940 2993 3361 3476 3477 3499 3536 3537 3729
Size: 53 368 115 1 22 37 1 192
Cuts at: 3729 524
Size: 3385
Fragments arranged by size:
3385 1128 422 368 363 261 192 150
115 63 53 37 28 22 1 1
1
EcoRI G'AATT_C
Cuts at: 3594 3594
Size: 6590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2793 4609 2793
Size: 1816 4774
FseI GG_CCGG'CC
Cuts at: 2874 2874
Size: 6590
FspI TGC'GCA
Cuts at: 1042 4371 1042
Size: 3329 3261
HaeII r_GCGC'y
Cuts at: 336 374 625 677 1253 1951 2196 3077 3249
Size: 38 251 52 576 698 245 881 172
Cuts at: 3249 4024 4272 5294 6273 336
Size: 775 248 1022 979 653
Fragments arranged by size:
1022 979 881 775 698 653 576 251
248 245 172 52 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1717 1749 2163 2195 2884 2916 4432 4462 4464
Size: 32 414 32 689 32 1516 30 2
Cuts at: 4464 4494 1717
Size: 30 3813
Fragments arranged by size:
3813 1516 689 414 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3629 3629
Size: 6590
KasI G'GCGC_C
Cuts at: 370 673 1249 3073 4020 4268 370
Size: 303 576 1824 947 248 2692
Fragments arranged by size:
2692 1824 947 576 303 248
KpnI G_GTAC'C
Cuts at: 3610 3610
Size: 6590
MluI A'CGCG_T
Cuts at: 6023 6023
Size: 6590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1097 2678 3116 5261 5445 1097
Size: 1581 438 2145 184 2242
Fragments arranged by size:
2242 2145 1581 438 184
MscI TGG'CCA
Cuts at: 2371 2497 3289 4351 2371
Size: 126 792 1062 4610
Fragments arranged by size:
4610 1062 792 126
MslI CAynn'nnrTG
Cuts at: 112 1609 1978 2134 2242 2413 4579 4706 112
Size: 1497 369 156 108 171 2166 127 1996
Fragments arranged by size:
2166 1996 1497 369 171 156 127 108
MspA1I CmG'CkG
Cuts at: 566 715 790 899 1046 1298 1461 1907 2100
Size: 149 75 109 147 252 163 446 193
Cuts at: 2100 3303 3387 3531 3701 4015 4375 5388 5633
Size: 1203 84 144 170 314 360 1013 245
Cuts at: 5633 566
Size: 1523
Fragments arranged by size:
1523 1203 1013 446 360 314 252 245
193 170 163 149 147 144 109 84
75
NaeI GCC'GGC
Cuts at: 2872 4771 2872
Size: 1899 4691
NarI GG'CG_CC
Cuts at: 371 674 1250 3074 4021 4269 371
Size: 303 576 1824 947 248 2692
Fragments arranged by size:
2692 1824 947 576 303 248
NcoI C'CATG_G
Cuts at: 758 758
Size: 6590
NgoMIV G'CCGG_C
Cuts at: 2870 4769 2870
Size: 1899 4691
NheI G'CTAG_C
Cuts at: 1797 2065 1797
Size: 268 6322
NspI r_CATG'y
Cuts at: 2395 2422 2640 3643 4143 5050 2395
Size: 27 218 1003 500 907 3935
Fragments arranged by size:
3935 1003 907 500 218 27
PasI CC'CwG_GG
Cuts at: 1245 1245
Size: 6590
PciI A'CATG_T
Cuts at: 2636 5046 2636
Size: 2410 4180
PflMI CCAn_nnn'nTGG
Cuts at: 566 1461 3450 566
Size: 895 1989 3706
Fragments arranged by size:
3706 1989 895
PfoI T'CCnGG_A
Cuts at: 149 149
Size: 6590
PmeI GTTT'AAAC
Cuts at: 3589 3589
Size: 6590
PmlI CAC'GTG
Cuts at: 771 771
Size: 6590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5729 5761 5729
Size: 32 6558
PpuMI rG'GwC_Cy
Cuts at: 524 814 1236 2427 524
Size: 290 422 1191 4687
Fragments arranged by size:
4687 1191 422 290
PshAI GACnn'nnGTC
Cuts at: 3325 3325
Size: 6590
PspOMI G'GGCC_C
Cuts at: 552 784 2363 2576 2940 3476 3536 552
Size: 232 1579 213 364 536 60 3606
Fragments arranged by size:
3606 1579 536 364 232 213 60
PspXI vC'TCGA_Gb
Cuts at: 2197 2197
Size: 6590
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2632 2664 2632
Size: 32 6558
PstI C_TGCA'G
Cuts at: 904 1280 2282 2321 3302 3637 4322 904
Size: 376 1002 39 981 335 685 3172
Fragments arranged by size:
3172 1002 981 685 376 335 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6590
PvuII CAG'CTG
Cuts at: 566 899 3303 3531 4015 4375 566
Size: 333 2404 228 484 360 2781
Fragments arranged by size:
2781 2404 484 360 333 228
RsrII CG'GwC_CG
Cuts at: 4785 4785
Size: 6590
SacI G_AGCT'C
Cuts at: 3143 3604 3143
Size: 461 6129
SacII CC_GC'GG
Cuts at: 716 791 716
Size: 75 6515
SalI G'TCGA_C
Cuts at: 3627 3627
Size: 6590
SanDI GG'GwC_CC
Cuts at: 524 814 524
Size: 290 6300
SapI GCTCTTCn'nnn_
Cuts at: 3037 4613 4823 3037
Size: 1576 210 4804
Fragments arranged by size:
4804 1576 210
SbfI CC_TGCA'GG
Cuts at: 2282 3637 2282
Size: 1355 5235
ScaI AGT'ACT
Cuts at: 2206 6107 2206
Size: 3901 2689
SfcI C'TryA_G
Cuts at: 33 900 1276 2278 2317 3298 3307 3339 3633
Size: 867 376 1002 39 981 9 32 294
Cuts at: 3633 4318 5311 5502 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1002 993 981 867 685 376 294
191 39 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 1916 2880 1916
Size: 964 5626
SfoI GGC'GCC
Cuts at: 372 675 1251 3075 4022 4270 372
Size: 303 576 1824 947 248 2692
Fragments arranged by size:
2692 1824 947 576 303 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
SmaI CCC'GGG
Cuts at: 634 680 862 2458 2714 3197 3211 3359 3473
Size: 46 182 1596 256 483 14 148 114
Cuts at: 3473 3578 3612 634
Size: 105 34 3612
Fragments arranged by size:
3612 1596 483 256 182 148 114 105
46 34 14
SmlI C'TyrA_G
Cuts at: 1135 2197 3745 4660 5152 5414 1135
Size: 1062 1548 915 492 262 2311
Fragments arranged by size:
2311 1548 1062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6590
SphI G_CATG'C
Cuts at: 2422 3643 4143 2422
Size: 1221 500 4869
Fragments arranged by size:
4869 1221 500
StuI AGG'CCT
Cuts at: 2550 2550
Size: 6590
StyI C'CwwG_G
Cuts at: 758 2348 2828 3419 3724 4078 758
Size: 1590 480 591 305 354 3270
Fragments arranged by size:
3270 1590 591 480 354 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 735 1049 1246 4173 4306 4765 4974 735
Size: 314 197 2927 133 459 209 2351
Fragments arranged by size:
2927 2351 459 314 209 197 133
TatI w'GTAC_w
Cuts at: 1268 2105 2204 2444 6105 1268
Size: 837 99 240 3661 1753
Fragments arranged by size:
3661 1753 837 240 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1274 1306 2206 2238 2650 2682 6107 6139 1274
Size: 32 900 32 412 32 3425 32 1725
Fragments arranged by size:
3425 1725 900 412 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2488 4387 2488
Size: 1899 4691
XbaI T'CTAG_A
Cuts at: 3621 3621
Size: 6590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 567 903 1548 1824 3417 4085 210
Size: 357 336 645 276 1593 668 2715
Fragments arranged by size:
2715 1593 668 645 357 336 276
XhoI C'TCGA_G
Cuts at: 2197 2197
Size: 6590
XmaI C'CCGG_G
Cuts at: 632 678 860 2456 2712 3195 3209 3357 3471
Size: 46 182 1596 256 483 14 148 114
Cuts at: 3471 3576 3610 632
Size: 105 34 3612
Fragments arranged by size:
3612 1596 483 256 182 148 114 105
46 34 14
XmnI GAAnn'nnTTC
Cuts at: 145 2023 2263 145
Size: 1878 240 4472
Fragments arranged by size:
4472 1878 240
ZraI GAC'GTC
Cuts at: 2487 2487
Size: 6590
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmI BsrGI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PsiI SexAI SgrAI SnaBI SrfI
SspI SwaI