Restriction map for
pF1KE2380
(Circular) (Six-base) MAPSORT of: pF1KE2380.seq Check: 6579 from: 1 to: 5000
pF1KE2380 5000 bp
With 182 enzymes: SgfI *
October 25, 2011 13:28 ..
AccI GT'mk_AC
Cuts at: 257 1427 2038 257
Size: 1170 611 3219
Fragments arranged by size:
3219 1170 611
Acc65I G'GTAC_C
Cuts at: 1400 2016 1400
Size: 616 4384
AclI AA'CG_TT
Cuts at: 4840 4840
Size: 5000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2825 3257 4004 4272 1342
Size: 1483 432 747 268 2070
Fragments arranged by size:
2070 1483 747 432 268
AflIII A'CryG_T
Cuts at: 2352 3456 4433 2352
Size: 1104 977 2919
Fragments arranged by size:
2919 1104 977
AgeI A'CCGG_T
Cuts at: 3388 3388
Size: 5000
AhdI GACnn_n'nnGTC
Cuts at: 1341 1341
Size: 5000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 906 139
Size: 767 4233
AlwNI CAG_nnn'CTG
Cuts at: 147 298 1711 2305 3872 147
Size: 151 1413 594 1567 1275
Fragments arranged by size:
1567 1413 1275 594 151
ApaLI G'TGCA_C
Cuts at: 3770 3770
Size: 5000
ApoI r'AATT_y
Cuts at: 1225 2004 4353 4609 1225
Size: 779 2349 256 1616
Fragments arranged by size:
2349 1616 779 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5000
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
AvaI C'yCGr_G
Cuts at: 226 374 1184 1281 1756 2020 226
Size: 148 810 97 475 264 3206
Fragments arranged by size:
3206 810 475 264 148 97
BamHI G'GATC_C
Cuts at: 2025 2025
Size: 5000
BanI G'GyrC_C
Cuts at: 1108 1285 1400 1858 2016 2430 2678 2713 1108
Size: 177 115 458 158 414 248 35 3395
Fragments arranged by size:
3395 458 414 248 177 158 115 35
BanII G_rGCy'C
Cuts at: 394 431 1135 2014 3044 394
Size: 37 704 879 1030 2350
Fragments arranged by size:
2350 1030 879 704 37
BbeI G_GCGC'C
Cuts at: 2434 2682 2434
Size: 248 4752
BbsI GAAGACnn'nnnn_
Cuts at: 1150 1150
Size: 5000
BbvCI CC'TCA_GC
Cuts at: 1205 1205
Size: 5000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 352 386 352
Size: 34 4966
BciVI GTATCCnnnnn_n'
Cuts at: 2898 3659 5000 2898
Size: 761 1341 2898
Fragments arranged by size:
2898 1341 761
BclI T'GATC_A
Cuts at: 614 2520 614
Size: 1906 3094
BglII A'GATC_T
Cuts at: 2515 4552 2515
Size: 2037 2963
BlpI GC'TnA_GC
Cuts at: 393 2112 393
Size: 1719 3281
Bme1580I G_kGCm'C
Cuts at: 1861 2625 2718 3774 1861
Size: 764 93 1056 3087
Fragments arranged by size:
3087 1056 764 93
BmrI ACTGGGnnnn_n'
Cuts at: 253 2212 2293 2628 4772 253
Size: 1959 81 335 2144 481
Fragments arranged by size:
2144 1959 481 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1548 3367 1548
Size: 1819 3181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 522 1767 290
Size: 232 1245 3523
Fragments arranged by size:
3523 1245 232
Bpu10I CC'TnA_GC
Cuts at: 1205 1813 1205
Size: 608 4392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 3055 3547 3845 2176
Size: 879 492 298 3331
Fragments arranged by size:
3331 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 525 4704 525
Size: 4179 821
BsaAI yAC'GTr
Cuts at: 1095 2353 1095
Size: 1258 3742
BsaBI GATnn'nnATC
Cuts at: 6 2514 4551 6
Size: 2508 2037 455
Fragments arranged by size:
2508 2037 455
BsaHI Gr'CG_yC
Cuts at: 982 1082 1739 1939 2431 2679 3381 4877 982
Size: 100 657 200 492 248 702 1496 1105
Fragments arranged by size:
1496 1105 702 657 492 248 200 100
BsaWI w'CCGG_w
Cuts at: 1868 2189 2710 3388 3662 3809 4220 1868
Size: 321 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 321 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 784 1708 1948 193
Size: 591 924 240 3245
Fragments arranged by size:
3245 924 591 240
BseYI C'CCAG_C
Cuts at: 1100 1499 1503 2425 3183 3760 4256 1100
Size: 399 4 922 758 577 496 1844
Fragments arranged by size:
1844 922 758 577 496 399 4
BsiEI CG_ry'CG
Cuts at: 104 2542 2588 3796 104
Size: 2438 46 1208 1308
Fragments arranged by size:
2438 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 375 431 1805 2014 2792 2982 3774 375
Size: 56 1374 209 778 190 792 1601
Fragments arranged by size:
1601 1374 792 778 209 190 56
BsmI GAATG_Cn'
Cuts at: 294 294
Size: 5000
BsmBI CGTCTCn'nnnn_
Cuts at: 175 802 175
Size: 627 4373
Bsp1286I G_dGCh'C
Cuts at: 375 394 431 1135 1805 1861 2014 2625 2718
Size: 19 37 704 670 56 153 611 93
Cuts at: 2718 2792 2982 3044 3774 375
Size: 74 190 62 730 1601
Fragments arranged by size:
1601 730 704 670 611 190 153 93
74 62 56 37 19
BspEI T'CCGG_A
Cuts at: 4220 4220
Size: 5000
BspHI T'CATG_A
Cuts at: 1037 1838 4176 1037
Size: 801 2338 1861
Fragments arranged by size:
2338 1861 801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2050 2264 2566 2947 2050
Size: 214 302 381 4103
Fragments arranged by size:
4103 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3292 3346 4830 4995 50
Size: 3242 54 1484 165 55
Fragments arranged by size:
3242 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2912 2912
Size: 5000
BsrFI r'CCGG_y
Cuts at: 445 2998 3179 3388 445
Size: 2553 181 209 2057
Fragments arranged by size:
2553 2057 209 181
BssSI C'ACGA_G
Cuts at: 3271 3629 3271
Size: 358 4642
BstAPI GCAn_nnn'nTGC
Cuts at: 144 147 298 1711 2377 144
Size: 3 151 1413 666 2767
Fragments arranged by size:
2767 1413 666 151 3
BstBI TT'CG_AA
Cuts at: 3361 3361
Size: 5000
BstXI CCAn_nnnn'nTGG
Cuts at: 969 1563 969
Size: 594 4406
BstYI r'GATC_y
Cuts at: 1 1458 2025 2492 2515 2850 3096 4108 4194
Size: 1457 567 467 23 335 246 1012 86
Cuts at: 4194 4206 4552 1
Size: 12 346 449
Fragments arranged by size:
1457 1012 567 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1428 1428
Size: 5000
BtgI C'CryG_G
Cuts at: 107 668 968 1127 1199 1727 107
Size: 561 300 159 72 528 3380
Fragments arranged by size:
3380 561 528 300 159 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 906 987 2950 3131 3294 906
Size: 81 1963 181 163 2612
Fragments arranged by size:
2612 1963 181 163 81
BtsI GCAGTG_nn'
Cuts at: 617 1127 2243 617
Size: 510 1116 3374
Fragments arranged by size:
3374 1116 510
DraI TTT'AAA
Cuts at: 1224 1999 1224
Size: 775 4225
DraIII CAC_nnn'GTG
Cuts at: 247 247
Size: 5000
DrdI GACnn_nn'nnGTC
Cuts at: 189 2706 3564 189
Size: 2517 858 1625
Fragments arranged by size:
2517 1625 858
EaeI y'GGCC_r
Cuts at: 665 1124 1436 2585 2759 3150 3177 4522 665
Size: 459 312 1149 174 391 27 1345 1143
Fragments arranged by size:
1345 1149 1143 459 391 312 174 27
EagI C'GGCC_G
Cuts at: 2585 2585
Size: 5000
EarI CTCTTCn'nnn_
Cuts at: 932 3023 3233 932
Size: 2091 210 2699
Fragments arranged by size:
2699 2091 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 1140 3208 3518 3664 4810 168
Size: 972 2068 310 146 1146 358
Fragments arranged by size:
2068 1146 972 358 310 146
EcoICRI GAG'CTC
Cuts at: 429 2012 429
Size: 1583 3417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 522 1342 1767 2825 3257 4004 4272 290
Size: 232 820 425 1058 432 747 268 1018
Fragments arranged by size:
1058 1018 820 747 432 425 268 232
EcoO109I rG'GnC_Cy
Cuts at: 295 378 497 2139 295
Size: 83 119 1642 3156
Fragments arranged by size:
3156 1642 119 83
EcoRI G'AATT_C
Cuts at: 2004 2004
Size: 5000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1806 3019 1806
Size: 1213 3787
FspI TGC'GCA
Cuts at: 2781 2781
Size: 5000
HaeII r_GCGC'y
Cuts at: 509 1684 2434 2682 3704 4683 509
Size: 1175 750 248 1022 979 826
Fragments arranged by size:
1175 1022 979 826 750 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1327 1359 1924 1956 2842 2872 2874 2904 1327
Size: 32 565 32 886 30 2 30 3423
Fragments arranged by size:
3423 886 565 32 32 30 30 2
HincII GTy'rAC
Cuts at: 675 2039 675
Size: 1364 3636
KasI G'GCGC_C
Cuts at: 2430 2678 2430
Size: 248 4752
KpnI G_GTAC'C
Cuts at: 1404 2020 1404
Size: 616 4384
MluI A'CGCG_T
Cuts at: 4433 4433
Size: 5000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 402 1472 3671 3855 402
Size: 1070 2199 184 1547
Fragments arranged by size:
2199 1547 1070 184
MscI TGG'CCA
Cuts at: 1438 2761 1438
Size: 1323 3677
MslI CAynn'nnrTG
Cuts at: 721 967 1036 1822 2989 3116 721
Size: 246 69 786 1167 127 2605
Fragments arranged by size:
2605 1167 786 246 127 69
MspA1I CmG'CkG
Cuts at: 1503 1848 2111 2425 2785 3798 4043 1503
Size: 345 263 314 360 1013 245 2460
Fragments arranged by size:
2460 1013 360 345 314 263 245
NaeI GCC'GGC
Cuts at: 3181 3181
Size: 5000
NarI GG'CG_CC
Cuts at: 2431 2679 2431
Size: 248 4752
NcoI C'CATG_G
Cuts at: 107 668 1199 107
Size: 561 531 3908
Fragments arranged by size:
3908 561 531
NgoMIV G'CCGG_C
Cuts at: 3179 3179
Size: 5000
NspI r_CATG'y
Cuts at: 2053 2553 3460 2053
Size: 500 907 3593
Fragments arranged by size:
3593 907 500
PciI A'CATG_T
Cuts at: 3456 3456
Size: 5000
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5000
PmlI CAC'GTG
Cuts at: 1095 1095
Size: 5000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4968
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 5000
PstI C_TGCA'G
Cuts at: 2047 2732 2047
Size: 685 4315
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5000
PvuII CAG'CTG
Cuts at: 1503 1848 2425 2785 1503
Size: 345 577 360 3718
Fragments arranged by size:
3718 577 360 345
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5000
SacI G_AGCT'C
Cuts at: 431 2014 431
Size: 1583 3417
SalI G'TCGA_C
Cuts at: 2037 2037
Size: 5000
SanDI GG'GwC_CC
Cuts at: 378 378
Size: 5000
SapI GCTCTTCn'nnn_
Cuts at: 3023 3233 3023
Size: 210 4790
SbfI CC_TGCA'GG
Cuts at: 2047 2047
Size: 5000
ScaI AGT'ACT
Cuts at: 1443 4517 1443
Size: 3074 1926
SexAI A'CCwGG_T
Cuts at: 1421 1421
Size: 5000
SfcI C'TryA_G
Cuts at: 33 168 405 2043 2728 3721 3912 33
Size: 135 237 1638 685 993 191 1121
Fragments arranged by size:
1638 1121 993 685 237 191 135
SfoI GGC'GCC
Cuts at: 2432 2680 2432
Size: 248 4752
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
SmaI CCC'GGG
Cuts at: 2022 2022
Size: 5000
SmlI C'TyrA_G
Cuts at: 2155 3070 3562 3824 2155
Size: 915 492 262 3331
Fragments arranged by size:
3331 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5000
SphI G_CATG'C
Cuts at: 2053 2553 2053
Size: 500 4500
SspI AAT'ATT
Cuts at: 683 683
Size: 5000
StyI C'CwwG_G
Cuts at: 107 668 1199 2134 2488 107
Size: 561 531 935 354 2619
Fragments arranged by size:
2619 935 561 531 354
SwaI ATTT'AAAT
Cuts at: 1224 1224
Size: 5000
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 235 1569 2583 2716 3175 3384 235
Size: 1334 1014 133 459 209 1851
Fragments arranged by size:
1851 1334 1014 459 209 133
TatI w'GTAC_w
Cuts at: 1441 4515 1441
Size: 3074 1926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 819 851 4517 4549 819
Size: 32 3666 32 1270
Fragments arranged by size:
3666 1270 32 32
Tth111I GACn'n_nGTC
Cuts at: 2797 2797
Size: 5000
XbaI T'CTAG_A
Cuts at: 2031 2031
Size: 5000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1119 2495 1119
Size: 1376 3624
XmaI C'CCGG_G
Cuts at: 2020 2020
Size: 5000
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsrGI BssHII BstEII Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI StuI XhoI XmnI ZraI