Restriction map for
pF1KE2383
(Circular) (Six-base) MAPSORT of: pF1KE2383.seq Check: 6668 from: 1 to: 4583
pF1KE2383 4583 bp
With 182 enzymes: SgfI *
October 27, 2011 13:18 ..
AccI GT'mk_AC
Cuts at: 233 1621 233
Size: 1388 3195
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 4423 4423
Size: 4583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 2408 2840 3587 3855 1567
Size: 841 432 747 268 2295
Fragments arranged by size:
2295 841 747 432 268
AfeI AGC'GCT
Cuts at: 1287 1287
Size: 4583
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1304 1304
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 786 882 1269 1480 1888 3455 786
Size: 96 387 211 408 1567 1914
Fragments arranged by size:
1914 1567 408 387 211 96
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 1220 1351 1587 3936 4192 1220
Size: 131 236 2349 256 1611
Fragments arranged by size:
2349 1611 256 236 131
AseI AT'TA_AT
Cuts at: 20 1097 20
Size: 1077 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 1048 1603 1048
Size: 555 4028
BamHI G'GATC_C
Cuts at: 521 1608 521
Size: 1087 3496
BanI G'GyrC_C
Cuts at: 692 1156 1186 1599 2013 2261 2296 692
Size: 464 30 413 414 248 35 2979
Fragments arranged by size:
2979 464 414 413 248 35 30
BanII G_rGCy'C
Cuts at: 978 1597 2627 978
Size: 619 1030 2934
Fragments arranged by size:
2934 1030 619
BbeI G_GCGC'C
Cuts at: 696 1160 2017 2265 696
Size: 464 857 248 3014
Fragments arranged by size:
3014 857 464 248
BbsI GAAGACnn'nnnn_
Cuts at: 431 1407 431
Size: 976 3607
BbvCI CC'TCA_GC
Cuts at: 921 921
Size: 4583
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BfrBI ATG'CAT
Cuts at: 372 612 372
Size: 240 4343
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 713 1695 713
Size: 982 3601
Bme1580I G_kGCm'C
Cuts at: 2208 2301 3357 2208
Size: 93 1056 3434
Fragments arranged by size:
3434 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1795 1876 2211 4355 1795
Size: 81 335 2144 2023
Fragments arranged by size:
2144 2023 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 654 2950 654
Size: 2296 2287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 798 1091 682
Size: 116 293 4174
Fragments arranged by size:
4174 293 116
Bpu10I CC'TnA_GC
Cuts at: 921 921
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1759 2638 3130 3428 346
Size: 1413 879 492 298 1501
Fragments arranged by size:
1501 1413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1352 4287 1352
Size: 2935 1648
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 693 1157 1196 2014 2262 2964 4460 693
Size: 464 39 818 248 702 1496 816
Fragments arranged by size:
1496 818 816 702 464 248 39
BsaWI w'CCGG_w
Cuts at: 257 1772 2293 2971 3245 3392 3803 257
Size: 1515 521 678 274 147 411 1037
Fragments arranged by size:
1515 1037 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1064 1094 1064
Size: 30 4553
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 801 801
Size: 4583
BseYI C'CCAG_C
Cuts at: 762 2008 2766 3343 3839 762
Size: 1246 758 577 496 1506
Fragments arranged by size:
1506 1246 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 328 1043 1466 1561 305
Size: 23 715 423 95 3327
Fragments arranged by size:
3327 715 423 95 23
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3379 104
Size: 2021 46 1208 1308
Fragments arranged by size:
2021 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1597 2375 2565 3357 1597
Size: 778 190 792 2823
Fragments arranged by size:
2823 792 778 190
BsmI GAATG_Cn'
Cuts at: 342 520 600 839 867 342
Size: 178 80 239 28 4058
Fragments arranged by size:
4058 239 178 80 28
Bsp1286I G_dGCh'C
Cuts at: 978 1597 2208 2301 2375 2565 2627 3357 978
Size: 619 611 93 74 190 62 730 2204
Fragments arranged by size:
2204 730 619 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 3759 3759
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 1633 1847 2149 2530 402
Size: 1231 214 302 381 2455
Fragments arranged by size:
2455 1231 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2875 2929 4413 4578 50
Size: 2825 54 1484 165 55
Fragments arranged by size:
2825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 590 2495 590
Size: 1905 2678
BsrFI r'CCGG_y
Cuts at: 2581 2762 2971 2581
Size: 181 209 4193
Fragments arranged by size:
4193 209 181
BsrGI T'GTAC_A
Cuts at: 1256 1256
Size: 4583
BssSI C'ACGA_G
Cuts at: 957 981 2854 3212 957
Size: 24 1873 358 2328
Fragments arranged by size:
2328 1873 358 24
BstAPI GCAn_nnn'nTGC
Cuts at: 595 1489 1960 595
Size: 894 471 3218
Fragments arranged by size:
3218 894 471
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 420 822 420
Size: 402 4181
BstYI r'GATC_y
Cuts at: 1 173 521 1608 2075 2098 2433 2679 3691
Size: 172 348 1087 467 23 335 246 1012
Cuts at: 3691 3777 3789 4135 1
Size: 86 12 346 449
Fragments arranged by size:
1087 1012 467 449 348 346 335 246
172 86 23 12
BtgI C'CryG_G
Cuts at: 626 989 1190 626
Size: 363 201 4019
Fragments arranged by size:
4019 363 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2533 2714 2877 2533
Size: 181 163 4239
Fragments arranged by size:
4239 181 163
BtsI GCAGTG_nn'
Cuts at: 340 734 1013 1826 340
Size: 394 279 813 3097
Fragments arranged by size:
3097 813 394 279
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 1474 2289 3147 1474
Size: 815 858 2910
Fragments arranged by size:
2910 858 815
EaeI y'GGCC_r
Cuts at: 501 2168 2342 2733 2760 4105 501
Size: 1667 174 391 27 1345 979
Fragments arranged by size:
1667 1345 979 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 645 2606 2816 645
Size: 1961 210 2412
Fragments arranged by size:
2412 1961 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 782 2791 3101 3247 4393 782
Size: 2009 310 146 1146 972
Fragments arranged by size:
2009 1146 972 310 146
EcoICRI GAG'CTC
Cuts at: 1595 1595
Size: 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 798 1091 1567 2408 2840 3587 3855 682
Size: 116 293 476 841 432 747 268 1410
Fragments arranged by size:
1410 841 747 476 432 293 268 116
EcoNI CCTnn'n_nnAGG
Cuts at: 799 1204 1345 799
Size: 405 141 4037
Fragments arranged by size:
4037 405 141
EcoO109I rG'GnC_Cy
Cuts at: 532 1722 532
Size: 1190 3393
EcoRI G'AATT_C
Cuts at: 1220 1587 1220
Size: 367 4216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 340 2602 340
Size: 2262 2321
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 696 793 968 1160 1289 2017 2265 3287 4266
Size: 97 175 192 129 728 248 1022 979
Cuts at: 4266 696
Size: 1013
Fragments arranged by size:
1022 1013 979 728 248 192 175 129
97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 360 392 477 509 2425 2455 2457 2487 360
Size: 32 85 32 1916 30 2 30 2456
Fragments arranged by size:
2456 1916 85 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
KasI G'GCGC_C
Cuts at: 692 1156 2013 2261 692
Size: 464 857 248 3014
Fragments arranged by size:
3014 857 464 248
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 147 170 437 1062 3254 3438 147
Size: 23 267 625 2192 184 1292
Fragments arranged by size:
2192 1292 625 267 184 23
MscI TGG'CCA
Cuts at: 503 2344 503
Size: 1841 2742
MslI CAynn'nnrTG
Cuts at: 157 385 445 481 2572 2699 157
Size: 228 60 36 2091 127 2041
Fragments arranged by size:
2091 2041 228 127 60 36
MspA1I CmG'CkG
Cuts at: 836 907 1545 1694 2008 2368 3381 3626 836
Size: 71 638 149 314 360 1013 245 1793
Fragments arranged by size:
1793 1013 638 360 314 245 149 71
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 4583
NarI GG'CG_CC
Cuts at: 693 1157 2014 2262 693
Size: 464 857 248 3014
Fragments arranged by size:
3014 857 464 248
NcoI C'CATG_G
Cuts at: 626 1190 626
Size: 564 4019
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 4583
NsiI A_TGCA'T
Cuts at: 374 614 374
Size: 240 4343
NspI r_CATG'y
Cuts at: 1636 2136 3043 1636
Size: 500 907 3176
Fragments arranged by size:
3176 907 500
PasI CC'CwG_GG
Cuts at: 969 969
Size: 4583
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PfoI T'CCnGG_A
Cuts at: 251 251
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4551
PpuMI rG'GwC_Cy
Cuts at: 532 532
Size: 4583
PshAI GACnn'nnGTC
Cuts at: 260 260
Size: 4583
PstI C_TGCA'G
Cuts at: 399 800 906 1277 1293 1376 1630 2315 399
Size: 401 106 371 16 83 254 685 2667
Fragments arranged by size:
2667 685 401 371 254 106 83 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 907 1545 2008 2368 907
Size: 638 463 360 3122
Fragments arranged by size:
3122 638 463 360
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1597 1597
Size: 4583
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 645 2606 2816 645
Size: 1961 210 2412
Fragments arranged by size:
2412 1961 210
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 395 796 902 1273 1289 1372 1626 2311
Size: 362 401 106 371 16 83 254 685
Cuts at: 2311 3304 3495 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 401 371 362 254 191
106 83 16
SfoI GGC'GCC
Cuts at: 694 1158 2015 2263 694
Size: 464 857 248 3014
Fragments arranged by size:
3014 857 464 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 325 1738 2653 3145 3407 325
Size: 1413 915 492 262 1501
Fragments arranged by size:
1501 1413 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
SspI AAT'ATT
Cuts at: 351 1095 351
Size: 744 3839
StuI AGG'CCT
Cuts at: 1210 1210
Size: 4583
StyI C'CwwG_G
Cuts at: 626 1190 1717 2071 626
Size: 564 527 354 3138
Fragments arranged by size:
3138 564 527 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 814 1219 2166 2299 2758 2967 814
Size: 405 947 133 459 209 2430
Fragments arranged by size:
2430 947 459 405 209 133
TatI w'GTAC_w
Cuts at: 1256 4098 1256
Size: 2842 1741
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4551
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1504 2078 1504
Size: 574 4009
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI