Restriction map for
pF1KE2389
(Circular) (Six-base) MAPSORT of: pF1KE2389.seq Check: 1037 from: 1 to: 5186
pF1KE2389 5186 bp
With 182 enzymes: SgfI *
January 6, 2012 11:52 ..
AccI GT'mk_AC
Cuts at: 1979 2224 1979
Size: 245 4941
Acc65I G'GTAC_C
Cuts at: 2202 2202
Size: 5186
AclI AA'CG_TT
Cuts at: 5026 5026
Size: 5186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 3011 3443 4190 4458 137
Size: 2874 432 747 268 865
Fragments arranged by size:
2874 865 747 432 268
AflIII A'CryG_T
Cuts at: 614 1770 1900 1933 2538 3642 4619 614
Size: 1156 130 33 605 1104 977 1181
Fragments arranged by size:
1181 1156 1104 977 605 130 33
AgeI A'CCGG_T
Cuts at: 3574 3574
Size: 5186
AlwNI CAG_nnn'CTG
Cuts at: 2491 4058 2491
Size: 1567 3619
ApaLI G'TGCA_C
Cuts at: 3956 3956
Size: 5186
ApoI r'AATT_y
Cuts at: 336 507 545 1303 1333 1705 1737 1859 2190
Size: 171 38 758 30 372 32 122 331
Cuts at: 2190 4539 4795 336
Size: 2349 256 727
Fragments arranged by size:
2349 758 727 372 331 256 171 122
38 32 30
AseI AT'TA_AT
Cuts at: 20 539 2035 20
Size: 519 1496 3171
Fragments arranged by size:
3171 1496 519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
AvaI C'yCGr_G
Cuts at: 2206 2206
Size: 5186
BamHI G'GATC_C
Cuts at: 370 2211 370
Size: 1841 3345
BanI G'GyrC_C
Cuts at: 1762 2202 2616 2864 2899 1762
Size: 440 414 248 35 4049
Fragments arranged by size:
4049 440 414 248 35
BanII G_rGCy'C
Cuts at: 1887 2200 3230 1887
Size: 313 1030 3843
Fragments arranged by size:
3843 1030 313
BbeI G_GCGC'C
Cuts at: 2620 2868 2620
Size: 248 4938
BbsI GAAGACnn'nnnn_
Cuts at: 1176 1985 1176
Size: 809 4377
BciVI GTATCCnnnnn_n'
Cuts at: 3084 3845 5186 3084
Size: 761 1341 3084
Fragments arranged by size:
3084 1341 761
BclI T'GATC_A
Cuts at: 2706 2706
Size: 5186
BfrBI ATG'CAT
Cuts at: 2080 2080
Size: 5186
BglII A'GATC_T
Cuts at: 828 2701 4738 828
Size: 1873 2037 1276
Fragments arranged by size:
2037 1873 1276
BlpI GC'TnA_GC
Cuts at: 2298 2298
Size: 5186
Bme1580I G_kGCm'C
Cuts at: 2811 2904 3960 2811
Size: 93 1056 4037
Fragments arranged by size:
4037 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1831 2398 2479 2814 4958 1831
Size: 567 81 335 2144 2059
Fragments arranged by size:
2144 2059 567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 3553 309
Size: 3244 1942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 1627
Size: 5186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2362 3241 3733 4031 2362
Size: 879 492 298 3517
Fragments arranged by size:
3517 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1645 4890 1645
Size: 3245 1941
BsaAI yAC'GTr
Cuts at: 810 1194 1936 2539 810
Size: 384 742 603 3457
Fragments arranged by size:
3457 742 603 384
BsaBI GATnn'nnATC
Cuts at: 6 2700 4737 6
Size: 2694 2037 455
Fragments arranged by size:
2694 2037 455
BsaHI Gr'CG_yC
Cuts at: 2617 2865 3567 5063 2617
Size: 248 702 1496 2740
Fragments arranged by size:
2740 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2375 2896 3574 3848 3995 4406 2375
Size: 521 678 274 147 411 3155
Fragments arranged by size:
3155 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2155 2611 3369 3946 4442 2155
Size: 456 758 577 496 2899
Fragments arranged by size:
2899 758 577 496 456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 1736
Size: 5186
BsiEI CG_ry'CG
Cuts at: 104 2728 2774 3982 104
Size: 2624 46 1208 1308
Fragments arranged by size:
2624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1851 2200 2978 3168 3960 1851
Size: 349 778 190 792 3077
Fragments arranged by size:
3077 792 778 349 190
BsmI GAATG_Cn'
Cuts at: 750 1681 750
Size: 931 4255
Bsp1286I G_dGCh'C
Cuts at: 1851 1887 2200 2811 2904 2978 3168 3230 3960
Size: 36 313 611 93 74 190 62 730
Cuts at: 3960 1851
Size: 3077
Fragments arranged by size:
3077 730 611 313 190 93 74 62
36
BspEI T'CCGG_A
Cuts at: 4406 4406
Size: 5186
BspHI T'CATG_A
Cuts at: 4362 4362
Size: 5186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1558 2236 2450 2752 3133 1558
Size: 678 214 302 381 3611
Fragments arranged by size:
3611 678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3478 3532 5016 5181 50
Size: 3428 54 1484 165 55
Fragments arranged by size:
3428 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 761 3098 186
Size: 575 2337 2274
Fragments arranged by size:
2337 2274 575
BsrFI r'CCGG_y
Cuts at: 3184 3365 3574 3184
Size: 181 209 4796
Fragments arranged by size:
4796 209 181
BsrGI T'GTAC_A
Cuts at: 1696 1696
Size: 5186
BssSI C'ACGA_G
Cuts at: 3457 3815 3457
Size: 358 4828
BstAPI GCAn_nnn'nTGC
Cuts at: 2563 2563
Size: 5186
BstBI TT'CG_AA
Cuts at: 3547 3547
Size: 5186
BstYI r'GATC_y
Cuts at: 1 370 787 828 2211 2678 2701 3036 3282
Size: 369 417 41 1383 467 23 335 246
Cuts at: 3282 4294 4380 4392 4738 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1383 1012 467 449 417 369 346 335
246 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3136 3317 3480 3136
Size: 181 163 4842
Fragments arranged by size:
4842 181 163
BtsI GCAGTG_nn'
Cuts at: 2429 2429
Size: 5186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1908 1943 1908
Size: 35 5151
DraI TTT'AAA
Cuts at: 596 1095 2185 596
Size: 499 1090 3597
Fragments arranged by size:
3597 1090 499
DraIII CAC_nnn'GTG
Cuts at: 238 2179 238
Size: 1941 3245
DrdI GACnn_nn'nnGTC
Cuts at: 2892 3750 2892
Size: 858 4328
EaeI y'GGCC_r
Cuts at: 2771 2945 3336 3363 4708 2771
Size: 174 391 27 1345 3249
Fragments arranged by size:
3249 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2771 2771
Size: 5186
EarI CTCTTCn'nnn_
Cuts at: 300 3209 3419 300
Size: 2909 210 2067
Fragments arranged by size:
2909 2067 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3394 3704 3850 4996 3394
Size: 310 146 1146 3584
Fragments arranged by size:
3584 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2198 2198
Size: 5186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 1627 3011 3443 4190 4458 137
Size: 1490 1384 432 747 268 865
Fragments arranged by size:
1490 1384 865 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2325 2325
Size: 5186
EcoRI G'AATT_C
Cuts at: 1859 2190 1859
Size: 331 4855
EcoRV GAT'ATC
Cuts at: 290 2073 290
Size: 1783 3403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 213 998 3205 213
Size: 785 2207 2194
Fragments arranged by size:
2207 2194 785
FspI TGC'GCA
Cuts at: 2967 2967
Size: 5186
HaeII r_GCGC'y
Cuts at: 2620 2868 3890 4869 2620
Size: 248 1022 979 2937
Fragments arranged by size:
2937 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1277 1309 3028 3058 3060 3090 1277
Size: 32 1719 30 2 30 3373
Fragments arranged by size:
3373 1719 32 30 30 2
HincII GTy'rAC
Cuts at: 2225 2225
Size: 5186
HindIII A'AGCT_T
Cuts at: 321 980 321
Size: 659 4527
KasI G'GCGC_C
Cuts at: 2616 2864 2616
Size: 248 4938
KpnI G_GTAC'C
Cuts at: 2206 2206
Size: 5186
MfeI C'AATT_G
Cuts at: 1497 1497
Size: 5186
MluI A'CGCG_T
Cuts at: 4619 4619
Size: 5186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 852 1302 3857 4041 852
Size: 450 2555 184 1997
Fragments arranged by size:
2555 1997 450 184
MscI TGG'CCA
Cuts at: 2947 2947
Size: 5186
MslI CAynn'nnrTG
Cuts at: 615 1463 1670 3175 3302 615
Size: 848 207 1505 127 2499
Fragments arranged by size:
2499 1505 848 207 127
MspA1I CmG'CkG
Cuts at: 2297 2611 2971 3984 4229 2297
Size: 314 360 1013 245 3254
Fragments arranged by size:
3254 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3367 3367
Size: 5186
NarI GG'CG_CC
Cuts at: 2617 2865 2617
Size: 248 4938
NcoI C'CATG_G
Cuts at: 107 107
Size: 5186
NgoMIV G'CCGG_C
Cuts at: 3365 3365
Size: 5186
NsiI A_TGCA'T
Cuts at: 2082 2082
Size: 5186
NspI r_CATG'y
Cuts at: 618 1000 1774 2239 2739 3646 618
Size: 382 774 465 500 907 2158
Fragments arranged by size:
2158 907 774 500 465 382
PacI TTA_AT'TAA
Cuts at: 539 539
Size: 5186
PasI CC'CwG_GG
Cuts at: 783 783
Size: 5186
PciI A'CATG_T
Cuts at: 614 1770 3642 614
Size: 1156 1872 2158
Fragments arranged by size:
2158 1872 1156
PflMI CCAn_nnn'nTGG
Cuts at: 772 1528 1836 772
Size: 756 308 4122
Fragments arranged by size:
4122 756 308
PmeI GTTT'AAAC
Cuts at: 2185 2185
Size: 5186
PmlI CAC'GTG
Cuts at: 1936 1936
Size: 5186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 4325
Size: 32 5154
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1639 1671 1639
Size: 32 5154
PstI C_TGCA'G
Cuts at: 1621 2233 2918 1621
Size: 612 685 3889
Fragments arranged by size:
3889 685 612
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5186
PvuII CAG'CTG
Cuts at: 2611 2971 2611
Size: 360 4826
RsrII CG'GwC_CG
Cuts at: 3381 3381
Size: 5186
SacI G_AGCT'C
Cuts at: 2200 2200
Size: 5186
SalI G'TCGA_C
Cuts at: 2223 2223
Size: 5186
SapI GCTCTTCn'nnn_
Cuts at: 3209 3419 3209
Size: 210 4976
SbfI CC_TGCA'GG
Cuts at: 2233 2233
Size: 5186
ScaI AGT'ACT
Cuts at: 4703 4703
Size: 5186
SfcI C'TryA_G
Cuts at: 33 1601 1617 2229 2914 3907 4098 33
Size: 1568 16 612 685 993 191 1121
Fragments arranged by size:
1568 1121 993 685 612 191 16
SfoI GGC'GCC
Cuts at: 2618 2866 2618
Size: 248 4938
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
SmaI CCC'GGG
Cuts at: 2208 2208
Size: 5186
SmlI C'TyrA_G
Cuts at: 2341 3256 3748 4010 2341
Size: 915 492 262 3517
Fragments arranged by size:
3517 915 492 262
SnaBI TAC'GTA
Cuts at: 1194 1194
Size: 5186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5186
SphI G_CATG'C
Cuts at: 2239 2739 2239
Size: 500 4686
SspI AAT'ATT
Cuts at: 1059 1059
Size: 5186
StyI C'CwwG_G
Cuts at: 107 2320 2674 107
Size: 2213 354 2619
Fragments arranged by size:
2619 2213 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1660 2769 2902 3361 3570 1660
Size: 1109 133 459 209 3276
Fragments arranged by size:
3276 1109 459 209 133
TatI w'GTAC_w
Cuts at: 1696 4701 1696
Size: 3005 2181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1811 1843 4703 4735 1811
Size: 32 2860 32 2262
Fragments arranged by size:
2860 2262 32 32
Tth111I GACn'n_nGTC
Cuts at: 2983 2983
Size: 5186
XbaI T'CTAG_A
Cuts at: 2217 2217
Size: 5186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 675 2681 675
Size: 2006 3180
XmaI C'CCGG_G
Cuts at: 2206 2206
Size: 5186
XmnI GAAnn'nnTTC
Cuts at: 193 981 193
Size: 788 4398
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoNI FseI FspAI HpaI
NdeI NheI NotI NruI PfoI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI