Restriction map for
pF1KE2391
(Circular) (Six-base) MAPSORT of: pF1KE2391.seq Check: 1808 from: 1 to: 4664
pF1KE2391 4664 bp
With 182 enzymes: SgfI *
January 26, 2012 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 546 546
Size: 4664
AccI GT'mk_AC
Cuts at: 506 628 1702 506
Size: 122 1074 3468
Fragments arranged by size:
3468 1074 122
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 404 734 1432 2489 2921 3668 3936 391
Size: 13 330 698 1057 432 747 268 1119
Fragments arranged by size:
1119 1057 747 698 432 330 268 13
AfeI AGC'GCT
Cuts at: 1651 1651
Size: 4664
AflIII A'CryG_T
Cuts at: 1187 2016 3120 4097 1187
Size: 829 1104 977 1754
Fragments arranged by size:
1754 1104 977 829
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AleI CACnn'nnGTG
Cuts at: 1129 1129
Size: 4664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1060 1147 1060
Size: 87 4577
AlwNI CAG_nnn'CTG
Cuts at: 1969 3536 1969
Size: 1567 3097
ApaI G_GGCC'C
Cuts at: 1227 1227
Size: 4664
ApaLI G'TGCA_C
Cuts at: 3434 3434
Size: 4664
ApoI r'AATT_y
Cuts at: 388 1668 4017 4273 388
Size: 1280 2349 256 779
Fragments arranged by size:
2349 1280 779 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 1684 1684
Size: 4664
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 197 870 1343 1680 2094 2342 2377 197
Size: 673 473 337 414 248 35 2484
Fragments arranged by size:
2484 673 473 414 337 248 35
BanII G_rGCy'C
Cuts at: 233 786 1181 1227 1678 2708 233
Size: 553 395 46 451 1030 2189
Fragments arranged by size:
2189 1030 553 451 395 46
BbeI G_GCGC'C
Cuts at: 1347 2098 2346 1347
Size: 751 248 3665
Fragments arranged by size:
3665 751 248
BbsI GAAGACnn'nnnn_
Cuts at: 900 943 969 900
Size: 43 26 4595
Fragments arranged by size:
4595 43 26
BciVI GTATCCnnnnn_n'
Cuts at: 1246 2562 3323 4664 1246
Size: 1316 761 1341 1246
Fragments arranged by size:
1341 1316 1246 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BglII A'GATC_T
Cuts at: 479 2179 4216 479
Size: 1700 2037 927
Fragments arranged by size:
2037 1700 927
BlpI GC'TnA_GC
Cuts at: 1776 1776
Size: 4664
Bme1580I G_kGCm'C
Cuts at: 875 1227 1561 2289 2382 3438 875
Size: 352 334 728 93 1056 2101
Fragments arranged by size:
2101 1056 728 352 334 93
BmrI ACTGGGnnnn_n'
Cuts at: 139 1876 1957 2292 4436 139
Size: 1737 81 335 2144 367
Fragments arranged by size:
2144 1737 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1183 472
Size: 711 3953
Bpu10I CC'TnA_GC
Cuts at: 649 649
Size: 4664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 607 1270 1840 2719 3211 3509 592
Size: 15 663 570 879 492 298 1747
Fragments arranged by size:
1747 879 663 570 492 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 313 1159 4368 313
Size: 846 3209 609
Fragments arranged by size:
3209 846 609
BsaAI yAC'GTr
Cuts at: 2017 2017
Size: 4664
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 1344 1540 2095 2343 3045 4541 1344
Size: 196 555 248 702 1496 1467
Fragments arranged by size:
1496 1467 702 555 248 196
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3326 3473 3884 1853
Size: 521 678 274 147 411 2633
Fragments arranged by size:
2633 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 1612 1642 283
Size: 30 1299 30 3305
Fragments arranged by size:
3305 1299 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 196 268 331 543 1251 1285 1492 193
Size: 3 72 63 212 708 34 207 3365
Fragments arranged by size:
3365 708 212 207 72 63 34 3
BseYI C'CCAG_C
Cuts at: 164 799 1120 1197 1213 1368 1388 1604 2089
Size: 635 321 77 16 155 20 216 485
Cuts at: 2089 2847 3424 3920 164
Size: 758 577 496 908
Fragments arranged by size:
908 758 635 577 496 485 321 216
155 77 20 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 1340 1660 1168
Size: 172 320 4172
Fragments arranged by size:
4172 320 172
BsiEI CG_ry'CG
Cuts at: 104 757 2206 2252 3460 104
Size: 653 1449 46 1208 1308
Fragments arranged by size:
1449 1308 1208 653 46
BsiHKAI G_wGCw'C
Cuts at: 233 350 884 962 1100 1181 1678 2456 2646
Size: 117 534 78 138 81 497 778 190
Cuts at: 2646 3438 233
Size: 792 1459
Fragments arranged by size:
1459 792 778 534 497 190 138 117
81 78
BsmI GAATG_Cn'
Cuts at: 384 384
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 233 350 786 875 884 962 1100 1181 1227
Size: 117 436 89 9 78 138 81 46
Cuts at: 1227 1561 1678 2289 2382 2456 2646 2708 3438
Size: 334 117 611 93 74 190 62 730
Cuts at: 3438 233
Size: 1459
Fragments arranged by size:
1459 730 611 436 334 190 138 117
117 93 89 81 78 74 62 46
9
BspEI T'CCGG_A
Cuts at: 3884 3884
Size: 4664
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 546 1714 1928 2230 2611 546
Size: 1168 214 302 381 2599
Fragments arranged by size:
2599 1168 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 551 1326 2956 3010 4494 4659 50
Size: 136 365 775 1630 54 1484 165 55
Fragments arranged by size:
1630 1484 775 365 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 365 752 756 918 1361 2662 2843 3052 365
Size: 387 4 162 443 1301 181 209 1977
Fragments arranged by size:
1977 1301 443 387 209 181 162 4
BssSI C'ACGA_G
Cuts at: 2935 3293 2935
Size: 358 4306
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 1065 1065
Size: 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 1017 1077 1017
Size: 60 4604
BstYI r'GATC_y
Cuts at: 1 479 1568 1689 2156 2179 2514 2760 3772
Size: 478 1089 121 467 23 335 246 1012
Cuts at: 3772 3858 3870 4216 1
Size: 86 12 346 449
Fragments arranged by size:
1089 1012 478 467 449 346 335 246
121 86 23 12
BstZ17I GTA'TAC
Cuts at: 629 629
Size: 4664
BtgI C'CryG_G
Cuts at: 107 239 689 1016 1070 107
Size: 132 450 327 54 3701
Fragments arranged by size:
3701 450 327 132 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2614 2795 2958 2614
Size: 181 163 4320
Fragments arranged by size:
4320 181 163
BtsI GCAGTG_nn'
Cuts at: 1302 1352 1907 1302
Size: 50 555 4059
Fragments arranged by size:
4059 555 50
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 632 754 758 947 1019 1337 2249 2423 2814
Size: 122 4 189 72 318 912 174 391
Cuts at: 2814 2841 4186 632
Size: 27 1345 1110
Fragments arranged by size:
1345 1110 912 391 318 189 174 122
72 27 4
EagI C'GGCC_G
Cuts at: 754 2249 754
Size: 1495 3169
EarI CTCTTCn'nnn_
Cuts at: 853 1054 1162 1405 2687 2897 853
Size: 201 108 243 1282 210 2620
Fragments arranged by size:
2620 1282 243 210 201 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 823 2872 3182 3328 4474 823
Size: 2049 310 146 1146 1013
Fragments arranged by size:
2049 1146 1013 310 146
EcoICRI GAG'CTC
Cuts at: 231 1179 1676 231
Size: 948 497 3219
Fragments arranged by size:
3219 948 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 404 472 734 1183 1432 2489 2921 3668
Size: 13 68 262 449 249 1057 432 747
Cuts at: 3668 3936 391
Size: 268 1119
Fragments arranged by size:
1119 1057 747 449 432 268 262 249
68 13
EcoNI CCTnn'n_nnAGG
Cuts at: 658 658
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 1223 1224 1620 1803 1223
Size: 1 396 183 4084
Fragments arranged by size:
4084 396 183 1
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 864 1173 2683 864
Size: 309 1510 2845
Fragments arranged by size:
2845 1510 309
FseI GG_CCGG'CC
Cuts at: 760 760
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 1347 1653 2098 2346 3368 4347 1347
Size: 306 445 248 1022 979 1664
Fragments arranged by size:
1664 1022 979 445 306 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 1303 1335 1612 1644 2506 2536 2538
Size: 32 346 32 277 32 862 30 2
Cuts at: 2538 2568 925
Size: 30 3021
Fragments arranged by size:
3021 862 346 277 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 768 900 1446 1703 768
Size: 132 546 257 3729
Fragments arranged by size:
3729 546 257 132
HpaI GTT'AAC
Cuts at: 1446 1446
Size: 4664
KasI G'GCGC_C
Cuts at: 1343 2094 2342 1343
Size: 751 248 3665
Fragments arranged by size:
3665 751 248
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 3335 3519 314
Size: 3021 184 1459
Fragments arranged by size:
3021 1459 184
MscI TGG'CCA
Cuts at: 949 1021 1339 2425 949
Size: 72 318 1086 3188
Fragments arranged by size:
3188 1086 318 72
MslI CAynn'nnrTG
Cuts at: 868 955 1015 1075 1129 1552 2653 2780 868
Size: 87 60 60 54 423 1101 127 2752
Fragments arranged by size:
2752 1101 423 127 87 60 60 54
MspA1I CmG'CkG
Cuts at: 164 1420 1490 1775 2089 2449 3462 3707 164
Size: 1256 70 285 314 360 1013 245 1121
Fragments arranged by size:
1256 1121 1013 360 314 285 245 70
NaeI GCC'GGC
Cuts at: 758 1363 2845 758
Size: 605 1482 2577
Fragments arranged by size:
2577 1482 605
NarI GG'CG_CC
Cuts at: 1344 2095 2343 1344
Size: 751 248 3665
Fragments arranged by size:
3665 751 248
NcoI C'CATG_G
Cuts at: 107 1016 1070 107
Size: 909 54 3701
Fragments arranged by size:
3701 909 54
NgoMIV G'CCGG_C
Cuts at: 756 1361 2843 756
Size: 605 1482 2577
Fragments arranged by size:
2577 1482 605
NspI r_CATG'y
Cuts at: 1191 1717 2217 3124 1191
Size: 526 500 907 2731
Fragments arranged by size:
2731 907 526 500
PasI CC'CwG_GG
Cuts at: 1219 1248 1219
Size: 29 4635
PciI A'CATG_T
Cuts at: 1187 3120 1187
Size: 1933 2731
PflMI CCAn_nnn'nTGG
Cuts at: 338 338
Size: 4664
PfoI T'CCnGG_A
Cuts at: 1160 1160
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 1620 1620
Size: 4664
PshAI GACnn'nnGTC
Cuts at: 1543 1543
Size: 4664
PspOMI G'GGCC_C
Cuts at: 1223 1223
Size: 4664
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 667 699 667
Size: 32 4632
PstI C_TGCA'G
Cuts at: 1580 1711 2396 1580
Size: 131 685 3848
Fragments arranged by size:
3848 685 131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 164 1420 1490 2089 2449 164
Size: 1256 70 599 360 2379
Fragments arranged by size:
2379 1256 599 360 70
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 233 1181 1678 233
Size: 948 497 3219
Fragments arranged by size:
3219 948 497
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SanDI GG'GwC_CC
Cuts at: 1620 1620
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 1054 1162 2687 2897 1054
Size: 108 1525 210 2821
Fragments arranged by size:
2821 1525 210 108
SbfI CC_TGCA'GG
Cuts at: 1580 1711 1580
Size: 131 4533
ScaI AGT'ACT
Cuts at: 1276 4181 1276
Size: 2905 1759
SexAI A'CCwGG_T
Cuts at: 902 902
Size: 4664
SfcI C'TryA_G
Cuts at: 33 1576 1707 2392 3385 3576 33
Size: 1543 131 685 993 191 1121
Fragments arranged by size:
1543 1121 993 685 191 131
SfoI GGC'GCC
Cuts at: 1345 2096 2344 1345
Size: 751 248 3665
Fragments arranged by size:
3665 751 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 1686 1686
Size: 4664
SmlI C'TyrA_G
Cuts at: 607 622 1285 1819 2734 3226 3488 607
Size: 15 663 534 915 492 262 1783
Fragments arranged by size:
1783 915 663 534 492 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
StyI C'CwwG_G
Cuts at: 107 668 1016 1070 1262 1798 2152 107
Size: 561 348 54 192 536 354 2619
Fragments arranged by size:
2619 561 536 354 348 192 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1134 1141 2247 2380 2839 3048 1134
Size: 7 1106 133 459 209 2750
Fragments arranged by size:
2750 1106 459 209 133 7
TatI w'GTAC_w
Cuts at: 524 1274 4179 524
Size: 750 2905 1009
Fragments arranged by size:
2905 1009 750
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 4181 4213 415
Size: 32 3734 32 866
Fragments arranged by size:
3734 866 32 32
Tth111I GACn'n_nGTC
Cuts at: 2461 2461
Size: 4664
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 768 786 1612 2159 768
Size: 18 826 547 3273
Fragments arranged by size:
3273 826 547 18
XmaI C'CCGG_G
Cuts at: 1684 1684
Size: 4664
XmnI GAAnn'nnTTC
Cuts at: 1156 1156
Size: 4664
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII Bsu36I ClaI CspCI DraIII EcoRV FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI